data_1639

#######################
#  Entry information  #
#######################

save_entry_information
   _Saveframe_category      entry_information

   _Entry_title            
;
Sequential 1H NMR Assignments and Secondary Structure of an IgG-Binding Domain 
from Protein G
;
   _BMRB_accession_number   1639
   _BMRB_flat_file_name     bmr1639.str
   _Entry_type              update
   _Submission_date         1995-07-31
   _Accession_date          1996-04-13
   _Entry_origination       BMRB
   _NMR_STAR_version        2.1.1
   _Experimental_method     NMR
   _Details                 .

   loop_
      _Author_ordinal
      _Author_family_name
      _Author_given_name
      _Author_middle_initials
      _Author_family_title

      1 Lian      Lu-Yun  .    . 
      2 Yang      J.      C.   . 
      3 Derrick   J.      P.   . 
      4 Sutcliffe Michael J.   . 
      5 Roberts   G.      C.K. . 
      6 Murphy    J.      P.   . 
      7 Goward    C.      R.   . 
      8 Atkinson  T.      .    . 

   stop_

   loop_
      _Saveframe_category_type
      _Saveframe_category_type_count

      assigned_chemical_shifts 1 

   stop_

   loop_
      _Data_type
      _Data_type_count

      "1H chemical shifts" 350 

   stop_

   loop_
      _Revision_date
      _Revision_keyword
      _Revision_author
      _Revision_detail

      2010-06-15 revision BMRB 'Complete natural source information'                    
      1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format'                
      1996-04-13 revision BMRB 'Link to the Protein Data Bank added'                    
      1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 
      1995-07-31 original BMRB 'Last release in original BMRB flat-file format'         

   stop_

save_


#############################
#  Citation for this entry  #
#############################

save_entry_citation
   _Saveframe_category           entry_citation

   _Citation_full               
;
Lian, Lu-Yun, Yang, J. C., Derrick, J. P., Sutcliffe, Michael J., 
 Roberts, G.C.K., Murphy, J. P., Goward, C. R., Atkinson, T., 
 "Sequential 1H NMR Assignments and Secondary Structure of an IgG-Binding 
 Domain from Protein G,"
 Biochemistry 30, 5335-5340 (1991).
;
   _Citation_title              
;
Sequential 1H NMR Assignments and Secondary Structure of an IgG-Binding Domain 
from Protein G
;
   _Citation_status              published
   _Citation_type                journal
   _CAS_abstract_code            .
   _MEDLINE_UI_code              .
   _PubMed_ID                    ?

   loop_
      _Author_ordinal
      _Author_family_name
      _Author_given_name
      _Author_middle_initials
      _Author_family_title

      1 Lian      Lu-Yun  .    . 
      2 Yang      J.      C.   . 
      3 Derrick   J.      P.   . 
      4 Sutcliffe Michael J.   . 
      5 Roberts   G.      C.K. . 
      6 Murphy    J.      P.   . 
      7 Goward    C.      R.   . 
      8 Atkinson  T.      .    . 

   stop_

   _Journal_abbreviation         Biochemistry
   _Journal_volume               30
   _Journal_issue                .
   _Journal_CSD                  .
   _Book_chapter_title           .
   _Book_volume                  .
   _Book_series                  .
   _Book_ISBN                    .
   _Conference_state_province    .
   _Conference_abstract_number   .
   _Page_first                   5335
   _Page_last                    5340
   _Year                         1991
   _Details                      .

save_


##################################
#  Molecular system description  #
##################################

save_system_IgG_Fc_region-binding_protein
   _Saveframe_category         molecular_system

   _Mol_system_name           'IgG Fc region-binding protein'
   _Enzyme_commission_number   .

   loop_
      _Mol_system_component_name
      _Mol_label

      'IgG Fc region-binding protein' $IgG_Fc_region-binding_protein 

   stop_

   _System_molecular_weight    .
   _System_oligomer_state      ?
   _System_paramagnetic        ?
   _System_thiol_state         .
   _Database_query_date        .
   _Details                    .

save_


    ########################
    #  Monomeric polymers  #
    ########################

save_IgG_Fc_region-binding_protein
   _Saveframe_category                          monomeric_polymer

   _Mol_type                                    polymer
   _Mol_polymer_class                           protein
   _Name_common                                'IgG Fc region-binding protein'
   _Name_variant                               'protein G, IgG-binding domain'
   _Molecular_mass                              .
   _Mol_thiol_state                             .
   _Details                                     .

   	##############################
   	#  Polymer residue sequence  #
   	##############################
   
      _Residue_count                               64
   _Mol_residue_sequence                       
;
LTPAVTTYKLVINGKTLKGE
TTTEAVDAATAEKVFKQYAN
DNGVDGEWTYDDATKTFTVT
EKPE
;

   loop_
      _Residue_seq_code
      _Residue_label

       1 LEU   2 THR   3 PRO   4 ALA   5 VAL 
       6 THR   7 THR   8 TYR   9 LYS  10 LEU 
      11 VAL  12 ILE  13 ASN  14 GLY  15 LYS 
      16 THR  17 LEU  18 LYS  19 GLY  20 GLU 
      21 THR  22 THR  23 THR  24 GLU  25 ALA 
      26 VAL  27 ASP  28 ALA  29 ALA  30 THR 
      31 ALA  32 GLU  33 LYS  34 VAL  35 PHE 
      36 LYS  37 GLN  38 TYR  39 ALA  40 ASN 
      41 ASP  42 ASN  43 GLY  44 VAL  45 ASP 
      46 GLY  47 GLU  48 TRP  49 THR  50 TYR 
      51 ASP  52 ASP  53 ALA  54 THR  55 LYS 
      56 THR  57 PHE  58 THR  59 VAL  60 THR 
      61 GLU  62 LYS  63 PRO  64 GLU 

   stop_

   _Sequence_homology_query_date                .
   _Sequence_homology_query_revised_last_date   2014-06-03

   loop_
      _Database_name
      _Database_accession_code
      _Database_entry_mol_name
      _Sequence_query_to_submitted_percentage
      _Sequence_subject_length
      _Sequence_identity
      _Sequence_positive
      _Sequence_homology_expectation_value

      PDB  1FCC     "Crystal Structure Of The C2 Fragment Of Streptococcal Protein G In Complex With The Fc Domain Of Human Igg"  87.50  56 100.00 100.00 1.81e-29 
      PDB  1UWX     "P1.2 Serosubtype Antigen Derived From N. Meningitidis Pora In Complex With Fab Fragment"                     98.44  63  98.41 100.00 3.37e-34 
      PDB  2IGG     "Determination Of The Solution Structures Of Domains Ii And Iii Of Protein G From Streptococcus By 1h Nmr"   100.00  64 100.00 100.00 1.08e-35 
      EMBL CAA37410 "Protein G' [Streptococcus sp. 'group G']"                                                                    75.00 185  97.92 100.00 1.95e-22 

   stop_

save_


    ####################
    #  Natural source  #
    ####################

save_natural_source
   _Saveframe_category   natural_source


   loop_
      _Mol_label
      _Organism_name_common
      _NCBI_taxonomy_ID
      _Superkingdom
      _Kingdom
      _Genus
      _Species
      _Strain

      $IgG_Fc_region-binding_protein . 1306 Bacteria . Streptococcus sp. G148 

   stop_

save_


    #########################
    #  Experimental source  #
    #########################

save_experimental_source
   _Saveframe_category   experimental_source


   loop_
      _Mol_label
      _Production_method
      _Host_organism_name_common
      _Genus
      _Species
      _Strain
      _Vector_name

      $IgG_Fc_region-binding_protein 'not available' . Escherichia coli TG2 . 

   stop_

save_


#####################################
#  Sample contents and methodology  #
#####################################
	 
    ########################
    #  Sample description  #
    ########################

save_sample_one
   _Saveframe_category   sample

   _Sample_type          solution
   _Details              .

save_


#########################
#  Experimental detail  #
#########################

    ##################################
    #  NMR Spectrometer definitions  #
    ##################################

save_spectrometer_list
   _Saveframe_category   NMR_spectrometer

   _Manufacturer         unknown
   _Model                unknown
   _Field_strength       0
   _Details             'spectrometer information not available'

save_


    #############################
    #  NMR applied experiments  #
    #############################

save__1
   _Saveframe_category   NMR_applied_experiment

   _Sample_label        $sample_one

save_


#######################
#  Sample conditions  #
#######################

save_sample_condition_set_one
   _Saveframe_category   sample_conditions

   _Details              .

   loop_
      _Variable_type
      _Variable_value
      _Variable_value_error
      _Variable_value_units

      pH            4.2 . na 
      temperature 298   . K  

   stop_

save_


####################
#  NMR parameters  #
####################

    ##############################
    #  Assigned chemical shifts  #
    ##############################

	################################
	#  Chemical shift referencing  #
	################################

save_chem_shift_reference_par_set_one
   _Saveframe_category   chemical_shift_reference

   _Details             'The chemical shift reference is not available at this time.'

save_


	###################################
	#  Assigned chemical shift lists  #
	###################################

###################################################################
#       Chemical Shift Ambiguity Index Value Definitions          #
#                                                                 #
# The values other than 1 are used for those atoms with different #
# chemical shifts that cannot be assigned to stereospecific atoms #
# or to specific residues or chains.                              #
#                                                                 #
#   Index Value            Definition                             #
#                                                                 #
#      1             Unique (including isolated methyl protons,   #
#                         geminal atoms, and geminal methyl       #
#                         groups with identical chemical shifts)  #
#                         (e.g. ILE HD11, HD12, HD13 protons)     #
#      2             Ambiguity of geminal atoms or geminal methyl #
#                         proton groups (e.g. ASP HB2 and HB3     #
#                         protons, LEU CD1 and CD2 carbons, or    #
#                         LEU HD11, HD12, HD13 and HD21, HD22,    #
#                         HD23 methyl protons)                    #
#      3             Aromatic atoms on opposite sides of          #
#                         symmetrical rings (e.g. TYR HE1 and HE2 #
#                         protons)                                #
#      4             Intraresidue ambiguities (e.g. LYS HG and    #
#                         HD protons or TRP HZ2 and HZ3 protons)  #
#      5             Interresidue ambiguities (LYS 12 vs. LYS 27) #
#      6             Intermolecular ambiguities (e.g. ASP 31 CA   #
#                         in monomer 1 and ASP 31 CA in monomer 2 #
#                         of an asymmetrical homodimer, duplex    #
#                         DNA assignments, or other assignments   #
#                         that may apply to atoms in one or more  #
#                         molecule in the molecular assembly)     #
#      9             Ambiguous, specific ambiguity not defined    #
#                                                                 #
###################################################################
save_chemical_shift_assignment_data_set_one
   _Saveframe_category               assigned_chemical_shifts

   _Details                          .

   loop_
      _Sample_label

      $sample_one 

   stop_

   _Sample_conditions_label         $sample_condition_set_one
   _Chem_shift_reference_set_label  $chem_shift_reference_par_set_one
   _Mol_system_component_name       'IgG Fc region-binding protein'
   _Text_data_format                 .
   _Text_data                        .

   loop_
      _Atom_shift_assign_ID
      _Residue_author_seq_code
      _Residue_seq_code
      _Residue_label
      _Atom_name
      _Atom_type
      _Chem_shift_value
      _Chem_shift_value_error
      _Chem_shift_ambiguity_code

        1 .  2 THR H    H  8.75 . 1 
        2 .  2 THR HA   H  4.72 . 1 
        3 .  2 THR HB   H  4.18 . 1 
        4 .  2 THR HG2  H  1.33 . 1 
        5 .  3 PRO HA   H  4.45 . 1 
        6 .  3 PRO HB2  H  1.97 . 2 
        7 .  3 PRO HB3  H  2.37 . 2 
        8 .  3 PRO HG2  H  2.05 . 1 
        9 .  3 PRO HG3  H  2.05 . 1 
       10 .  3 PRO HD2  H  3.79 . 2 
       11 .  3 PRO HD3  H  3.95 . 2 
       12 .  4 ALA H    H  8.45 . 1 
       13 .  4 ALA HA   H  4.41 . 1 
       14 .  4 ALA HB   H  1.11 . 1 
       15 .  5 VAL H    H  7.97 . 1 
       16 .  5 VAL HA   H  4.33 . 1 
       17 .  5 VAL HB   H  1.91 . 1 
       18 .  5 VAL HG1  H   .87 . 1 
       19 .  5 VAL HG2  H   .87 . 1 
       20 .  6 THR H    H  8.47 . 1 
       21 .  6 THR HA   H  4.54 . 1 
       22 .  6 THR HB   H  4.08 . 1 
       23 .  6 THR HG2  H   .83 . 1 
       24 .  7 THR H    H  8.24 . 1 
       25 .  7 THR HA   H  4.85 . 1 
       26 .  7 THR HB   H  3.99 . 1 
       27 .  7 THR HG2  H  1.28 . 1 
       28 .  8 TYR H    H  9.37 . 1 
       29 .  8 TYR HA   H  5.38 . 1 
       30 .  8 TYR HB2  H  3.42 . 2 
       31 .  8 TYR HB3  H  2.95 . 2 
       32 .  8 TYR HD1  H  7.28 . 1 
       33 .  8 TYR HD2  H  7.28 . 1 
       34 .  8 TYR HE1  H  7.13 . 1 
       35 .  8 TYR HE2  H  7.13 . 1 
       36 .  9 LYS H    H  9.15 . 1 
       37 .  9 LYS HA   H  5.29 . 1 
       38 .  9 LYS HB2  H  1.99 . 2 
       39 .  9 LYS HB3  H  2.02 . 2 
       40 .  9 LYS HG2  H  1.5  . 2 
       41 .  9 LYS HG3  H  1.41 . 2 
       42 .  9 LYS HD2  H  1.64 . 1 
       43 .  9 LYS HD3  H  1.64 . 1 
       44 .  9 LYS HE2  H  2.85 . 2 
       45 .  9 LYS HE3  H  2.88 . 2 
       46 . 10 LEU H    H  8.7  . 1 
       47 . 10 LEU HA   H  5.05 . 1 
       48 . 10 LEU HB2  H  1.19 . 2 
       49 . 10 LEU HB3  H   .95 . 2 
       50 . 10 LEU HG   H   .86 . 1 
       51 . 10 LEU HD1  H   .44 . 2 
       52 . 10 LEU HD2  H   .54 . 2 
       53 . 11 VAL H    H  9.21 . 1 
       54 . 11 VAL HA   H  4.31 . 1 
       55 . 11 VAL HB   H  2    . 1 
       56 . 11 VAL HG1  H   .88 . 1 
       57 . 11 VAL HG2  H   .88 . 1 
       58 . 12 ILE H    H  8.74 . 1 
       59 . 12 ILE HA   H  4.3  . 1 
       60 . 12 ILE HB   H  1.65 . 1 
       61 . 12 ILE HG12 H  1.41 . 1 
       62 . 12 ILE HG13 H  1.41 . 1 
       63 . 12 ILE HG2  H   .76 . 1 
       64 . 12 ILE HD1  H   .69 . 1 
       65 . 13 ASN H    H  8.96 . 1 
       66 . 13 ASN HA   H  5.23 . 1 
       67 . 13 ASN HB2  H  2.6  . 2 
       68 . 13 ASN HB3  H  2.95 . 2 
       69 . 13 ASN HD21 H  7.21 . 2 
       70 . 13 ASN HD22 H  6.85 . 2 
       71 . 14 GLY H    H  7.88 . 1 
       72 . 14 GLY HA2  H  4.11 . 2 
       73 . 14 GLY HA3  H  4.35 . 2 
       74 . 15 LYS H    H  8.85 . 1 
       75 . 15 LYS HA   H  4.17 . 1 
       76 . 15 LYS HB2  H  1.88 . 1 
       77 . 15 LYS HB3  H  1.88 . 1 
       78 . 15 LYS HG2  H  1.51 . 1 
       79 . 15 LYS HG3  H  1.51 . 1 
       80 . 15 LYS HD2  H  1.76 . 1 
       81 . 15 LYS HD3  H  1.76 . 1 
       82 . 15 LYS HE2  H  3.03 . 1 
       83 . 15 LYS HE3  H  3.03 . 1 
       84 . 16 THR H    H  8.65 . 1 
       85 . 16 THR HA   H  4.41 . 1 
       86 . 16 THR HB   H  4.22 . 1 
       87 . 16 THR HG2  H  1.2  . 1 
       88 . 17 LEU H    H  7.93 . 1 
       89 . 17 LEU HA   H  4.32 . 1 
       90 . 17 LEU HB2  H  1.71 . 1 
       91 . 17 LEU HB3  H  1.71 . 1 
       92 . 17 LEU HG   H  1.43 . 1 
       93 . 17 LEU HD1  H   .86 . 2 
       94 . 17 LEU HD2  H   .93 . 2 
       95 . 18 LYS H    H  8.06 . 1 
       96 . 18 LYS HA   H  4.85 . 1 
       97 . 18 LYS HB2  H  1.79 . 2 
       98 . 18 LYS HB3  H  1.86 . 2 
       99 . 18 LYS HG2  H  1.53 . 1 
      100 . 18 LYS HG3  H  1.53 . 1 
      101 . 18 LYS HD2  H  1.79 . 1 
      102 . 18 LYS HD3  H  1.79 . 1 
      103 . 18 LYS HE2  H  3.04 . 1 
      104 . 18 LYS HE3  H  3.04 . 1 
      105 . 19 GLY H    H  8.3  . 1 
      106 . 19 GLY HA2  H  4.21 . 2 
      107 . 19 GLY HA3  H  4.26 . 2 
      108 . 20 GLU H    H  8.4  . 1 
      109 . 20 GLU HA   H  5.68 . 1 
      110 . 20 GLU HB2  H  2.02 . 2 
      111 . 20 GLU HB3  H  2.07 . 2 
      112 . 20 GLU HG2  H  2.31 . 2 
      113 . 20 GLU HG3  H  2.35 . 2 
      114 . 21 THR H    H  8.81 . 1 
      115 . 21 THR HA   H  4.78 . 1 
      116 . 21 THR HB   H  4.02 . 1 
      117 . 21 THR HG2  H   .63 . 1 
      118 . 22 THR H    H  8.18 . 1 
      119 . 22 THR HA   H  5.91 . 1 
      120 . 22 THR HB   H  4.4  . 1 
      121 . 22 THR HG2  H  1.25 . 1 
      122 . 23 THR H    H  9.1  . 1 
      123 . 23 THR HA   H  4.71 . 1 
      124 . 23 THR HB   H  3.91 . 1 
      125 . 23 THR HG2  H   .52 . 1 
      126 . 24 GLU H    H  8.08 . 1 
      127 . 24 GLU HA   H  5.5  . 1 
      128 . 24 GLU HB2  H  1.79 . 2 
      129 . 24 GLU HB3  H  2.05 . 2 
      130 . 24 GLU HG2  H  2.35 . 2 
      131 . 24 GLU HG3  H  2.41 . 2 
      132 . 25 ALA H    H  9.39 . 1 
      133 . 25 ALA HA   H  5.03 . 1 
      134 . 25 ALA HB   H  1.43 . 1 
      135 . 26 VAL H    H  8.7  . 1 
      136 . 26 VAL HA   H  4.43 . 1 
      137 . 26 VAL HB   H  2.28 . 1 
      138 . 26 VAL HG1  H  1.05 . 1 
      139 . 26 VAL HG2  H  1.05 . 1 
      140 . 27 ASP H    H  7.41 . 1 
      141 . 27 ASP HA   H  4.82 . 1 
      142 . 27 ASP HB2  H  3.08 . 1 
      143 . 27 ASP HB3  H  3.08 . 1 
      144 . 28 ALA H    H  8.45 . 1 
      145 . 28 ALA HA   H  3.37 . 1 
      146 . 28 ALA HB   H  1.23 . 1 
      147 . 29 ALA H    H  8.14 . 1 
      148 . 29 ALA HA   H  4    . 1 
      149 . 29 ALA HB   H  1.35 . 1 
      150 . 30 THR H    H  8.35 . 1 
      151 . 30 THR HA   H  3.76 . 1 
      152 . 30 THR HB   H  4.06 . 1 
      153 . 30 THR HG2  H  1.28 . 1 
      154 . 31 ALA H    H  7.19 . 1 
      155 . 31 ALA HA   H  3.19 . 1 
      156 . 31 ALA HB   H   .61 . 1 
      157 . 32 GLU H    H  8.44 . 1 
      158 . 32 GLU HA   H  2.64 . 1 
      159 . 32 GLU HB2  H  1.73 . 2 
      160 . 32 GLU HB3  H  1.82 . 2 
      161 . 32 GLU HG2  H  1.57 . 2 
      162 . 32 GLU HG3  H  1.92 . 2 
      163 . 33 LYS H    H  7.11 . 1 
      164 . 33 LYS HA   H  3.82 . 1 
      165 . 33 LYS HB2  H  1.88 . 1 
      166 . 33 LYS HB3  H  1.88 . 1 
      167 . 33 LYS HG2  H  1.36 . 1 
      168 . 33 LYS HG3  H  1.36 . 1 
      169 . 33 LYS HD2  H  1.57 . 2 
      170 . 33 LYS HD3  H  1.65 . 2 
      171 . 33 LYS HE2  H  2.94 . 1 
      172 . 33 LYS HE3  H  2.94 . 1 
      173 . 34 VAL H    H  7.36 . 1 
      174 . 34 VAL HA   H  3.66 . 1 
      175 . 34 VAL HB   H  1.83 . 1 
      176 . 34 VAL HG1  H   .96 . 2 
      177 . 34 VAL HG2  H   .85 . 2 
      178 . 35 PHE H    H  8.52 . 1 
      179 . 35 PHE HA   H  4.85 . 1 
      180 . 35 PHE HB2  H  2.92 . 2 
      181 . 35 PHE HB3  H  3.46 . 2 
      182 . 35 PHE HD1  H  6.58 . 1 
      183 . 35 PHE HD2  H  6.58 . 1 
      184 . 35 PHE HE1  H  7.06 . 1 
      185 . 35 PHE HE2  H  7.06 . 1 
      186 . 35 PHE HZ   H  7.21 . 1 
      187 . 36 LYS H    H  9.19 . 1 
      188 . 36 LYS HA   H  4.18 . 1 
      189 . 36 LYS HB2  H  1.72 . 1 
      190 . 36 LYS HB3  H  1.72 . 1 
      191 . 36 LYS HG2  H  1.6  . 1 
      192 . 36 LYS HG3  H  1.6  . 1 
      193 . 36 LYS HD2  H  1.75 . 1 
      194 . 36 LYS HD3  H  1.75 . 1 
      195 . 36 LYS HE2  H  3.05 . 1 
      196 . 36 LYS HE3  H  3.05 . 1 
      197 . 37 GLN H    H  7.57 . 1 
      198 . 37 GLN HA   H  4.11 . 1 
      199 . 37 GLN HB2  H  2.26 . 2 
      200 . 37 GLN HB3  H  2.3  . 2 
      201 . 37 GLN HG2  H  2.47 . 2 
      202 . 37 GLN HG3  H  2.5  . 2 
      203 . 37 GLN HE21 H  6.95 . 2 
      204 . 37 GLN HE22 H  7.89 . 2 
      205 . 38 TYR H    H  8.25 . 1 
      206 . 38 TYR HA   H  4.33 . 1 
      207 . 38 TYR HB2  H  3.37 . 1 
      208 . 38 TYR HB3  H  3.37 . 1 
      209 . 38 TYR HD1  H  7.01 . 1 
      210 . 38 TYR HD2  H  7.01 . 1 
      211 . 38 TYR HE1  H  6.73 . 1 
      212 . 38 TYR HE2  H  6.73 . 1 
      213 . 39 ALA H    H  9.24 . 1 
      214 . 39 ALA HA   H  3.84 . 1 
      215 . 39 ALA HB   H  1.87 . 1 
      216 . 40 ASN H    H  8.48 . 1 
      217 . 40 ASN HA   H  4.53 . 1 
      218 . 40 ASN HB2  H  2.98 . 2 
      219 . 40 ASN HB3  H  3    . 2 
      220 . 40 ASN HD21 H  7.14 . 2 
      221 . 40 ASN HD22 H  7.79 . 2 
      222 . 41 ASP H    H  8.83 . 1 
      223 . 41 ASP HA   H  4.21 . 1 
      224 . 41 ASP HB2  H  2.83 . 2 
      225 . 41 ASP HB3  H  2.71 . 2 
      226 . 42 ASN H    H  7.43 . 1 
      227 . 42 ASN HA   H  4.72 . 1 
      228 . 42 ASN HB2  H  2.11 . 2 
      229 . 42 ASN HB3  H  2.77 . 2 
      230 . 42 ASN HD21 H  6.56 . 2 
      231 . 42 ASN HD22 H  6.75 . 2 
      232 . 43 GLY H    H  7.78 . 1 
      233 . 43 GLY HA2  H  3.98 . 1 
      234 . 43 GLY HA3  H  3.98 . 1 
      235 . 44 VAL H    H  8.24 . 1 
      236 . 44 VAL HA   H  4.12 . 1 
      237 . 44 VAL HB   H  1.88 . 1 
      238 . 44 VAL HG1  H   .83 . 2 
      239 . 44 VAL HG2  H   .73 . 2 
      240 . 45 ASP H    H  8.57 . 1 
      241 . 45 ASP HA   H  5    . 1 
      242 . 45 ASP HB2  H  2.82 . 2 
      243 . 45 ASP HB3  H  2.71 . 2 
      244 . 46 GLY H    H  8.21 . 1 
      245 . 46 GLY HA2  H  3.87 . 2 
      246 . 46 GLY HA3  H  4.21 . 2 
      247 . 47 GLU H    H  8.15 . 1 
      248 . 47 GLU HA   H  4.84 . 1 
      249 . 47 GLU HB2  H  2.05 . 2 
      250 . 47 GLU HB3  H  2.16 . 2 
      251 . 47 GLU HG2  H  2.41 . 2 
      252 . 47 GLU HG3  H  2.48 . 2 
      253 . 48 TRP H    H  9.39 . 1 
      254 . 48 TRP HA   H  5.37 . 1 
      255 . 48 TRP HB2  H  3.22 . 2 
      256 . 48 TRP HB3  H  3.34 . 2 
      257 . 48 TRP HD1  H  7.6  . 1 
      258 . 48 TRP HE1  H 10.55 . 1 
      259 . 48 TRP HE3  H  7.6  . 1 
      260 . 48 TRP HZ2  H  7.33 . 1 
      261 . 48 TRP HZ3  H  6.65 . 1 
      262 . 48 TRP HH2  H  6.77 . 1 
      263 . 49 THR H    H  9.33 . 1 
      264 . 49 THR HA   H  4.88 . 1 
      265 . 49 THR HB   H  4.31 . 1 
      266 . 49 THR HG2  H  1.26 . 1 
      267 . 50 TYR H    H  8.66 . 1 
      268 . 50 TYR HA   H  5.05 . 1 
      269 . 50 TYR HB2  H  2.6  . 2 
      270 . 50 TYR HB3  H  2.93 . 2 
      271 . 50 TYR HD1  H  6.05 . 1 
      272 . 50 TYR HD2  H  6.05 . 1 
      273 . 50 TYR HE1  H  6.47 . 1 
      274 . 50 TYR HE2  H  6.47 . 1 
      275 . 51 ASP H    H  7.61 . 1 
      276 . 51 ASP HA   H  4.64 . 1 
      277 . 51 ASP HB2  H  2.69 . 2 
      278 . 51 ASP HB3  H  2.34 . 2 
      279 . 52 ASP H    H  8.62 . 1 
      280 . 52 ASP HA   H  4.17 . 1 
      281 . 52 ASP HB2  H  2.6  . 2 
      282 . 52 ASP HB3  H  2.88 . 2 
      283 . 53 ALA H    H  8.34 . 1 
      284 . 53 ALA HA   H  4.17 . 1 
      285 . 53 ALA HB   H  1.55 . 1 
      286 . 54 THR H    H  7.02 . 1 
      287 . 54 THR HA   H  4.43 . 1 
      288 . 54 THR HB   H  4.43 . 1 
      289 . 54 THR HG2  H  1.04 . 1 
      290 . 55 LYS H    H  7.88 . 1 
      291 . 55 LYS HA   H  4.24 . 1 
      292 . 55 LYS HB2  H  2.08 . 1 
      293 . 55 LYS HB3  H  2.08 . 1 
      294 . 55 LYS HG2  H  1.91 . 1 
      295 . 55 LYS HG3  H  1.91 . 1 
      296 . 56 THR H    H  7.42 . 1 
      297 . 56 THR HA   H  5.5  . 1 
      298 . 56 THR HB   H  3.79 . 1 
      299 . 56 THR HG2  H  1.01 . 1 
      300 . 57 PHE H    H 10.45 . 1 
      301 . 57 PHE HA   H  5.69 . 1 
      302 . 57 PHE HB2  H  3.23 . 2 
      303 . 57 PHE HB3  H  3.34 . 2 
      304 . 57 PHE HD1  H  7.74 . 1 
      305 . 57 PHE HD2  H  7.74 . 1 
      306 . 57 PHE HE1  H  7.11 . 1 
      307 . 57 PHE HE2  H  7.11 . 1 
      308 . 57 PHE HZ   H  6.96 . 1 
      309 . 58 THR H    H  9.19 . 1 
      310 . 58 THR HA   H  5.31 . 1 
      311 . 58 THR HB   H  3.85 . 1 
      312 . 58 THR HG2  H   .98 . 1 
      313 . 59 VAL H    H  8.54 . 1 
      314 . 59 VAL HA   H  4.55 . 1 
      315 . 59 VAL HB   H   .05 . 1 
      316 . 59 VAL HG1  H   .36 . 2 
      317 . 59 VAL HG2  H   .42 . 2 
      318 . 60 THR H    H  8.48 . 1 
      319 . 60 THR HA   H  4.77 . 1 
      320 . 60 THR HB   H  3.93 . 1 
      321 . 60 THR HG2  H  1.24 . 1 
      322 . 61 GLU H    H  8.29 . 1 
      323 . 61 GLU HA   H  4.54 . 1 
      324 . 61 GLU HB2  H  2.09 . 2 
      325 . 61 GLU HB3  H  2.21 . 2 
      326 . 61 GLU HG2  H  2.4  . 2 
      327 . 61 GLU HG3  H  2.48 . 2 
      328 . 62 LYS H    H  8.73 . 1 
      329 . 62 LYS HA   H  4.63 . 1 
      330 . 62 LYS HB2  H  1.89 . 2 
      331 . 62 LYS HB3  H  1.9  . 2 
      332 . 62 LYS HG2  H  1.57 . 1 
      333 . 62 LYS HG3  H  1.57 . 1 
      334 . 62 LYS HD2  H  1.76 . 1 
      335 . 62 LYS HD3  H  1.76 . 1 
      336 . 62 LYS HE2  H  3.04 . 1 
      337 . 62 LYS HE3  H  3.04 . 1 
      338 . 63 PRO HA   H  4.46 . 1 
      339 . 63 PRO HB2  H  2    . 2 
      340 . 63 PRO HB3  H  2.32 . 2 
      341 . 63 PRO HG2  H  2.11 . 1 
      342 . 63 PRO HG3  H  2.11 . 1 
      343 . 63 PRO HD2  H  3.75 . 2 
      344 . 63 PRO HD3  H  3.95 . 2 
      345 . 64 GLU H    H  7.99 . 1 
      346 . 64 GLU HA   H  4.22 . 1 
      347 . 64 GLU HB2  H  1.96 . 2 
      348 . 64 GLU HB3  H  2.14 . 2 
      349 . 64 GLU HG2  H  2.38 . 2 
      350 . 64 GLU HG3  H  2.41 . 2 

   stop_

save_