data_16417 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; An Atomic Resolution Model for Assembly, Architecture, and Function of the Dr Adhesins ; _BMRB_accession_number 16417 _BMRB_flat_file_name bmr16417.str _Entry_type original _Submission_date 2009-07-23 _Accession_date 2009-07-23 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Anderson Kirstine L. . 2 Billington Jason . . 3 Pettigrew David . . 4 Cota Ernesto . . 5 Simpson Peter . . 6 Roversi Pietro . . 7 Chen 'Ho An' . . 8 Urvil Petri . . 9 'du Merle' Laurence . . 10 Barlow Peter N. . 11 Medof M. E. . 12 Smith Richard A.G. . 13 Nowicki Bogdan . . 14 'Le Bouguenec' Chantal . . 15 Lea Susan M. . 16 Matthews Stephen . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count binding_constants 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-03-08 original author . stop_ _Original_release_date 2010-03-08 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'An Atomic Resolution Model for Assembly, Architecture, and Function of the Dr Adhsins' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 15327779 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Anderson Kirstine L. . 2 Billington Jason . . 3 Pettigrew David . . 4 Cota Ernesto . . 5 Urvil Petri . . 6 Simpson Peter . . 7 Roversi Pietro . . 8 Chen 'Ho An' . . 9 'du Merle' Laurence . . 10 Barlow Paul N. . 11 Medof M. E. . 12 Smith Richard A.G. . 13 Nowicki Bogdan . . 14 'Le Bouguenec' Chantal . . 15 Lea Susan M. . 16 Matthews Stephen . . stop_ _Journal_abbreviation 'Mol. Cell' _Journal_name_full 'Molecular Cell' _Journal_volume 15 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 647 _Page_last 657 _Year 2004 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'AfaE-dsc_DAF1234 complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label AfaE-dsc $AfaE-dsc DAF1234 $DAF1234 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_AfaE-dsc _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common AfaE-dsc _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 143 _Mol_residue_sequence ; EECQVRVGDLTVAKTRGQLT DAAPIGPVTVQALGCNARQV ALKADTDNFEQGKFFLISDN NRDKLYVNIRPMDNSAWTTD NGVFYKNDVGSWGGTIGIYV DGQQTNTPPGNYTLTLTGGY WAKDNKQGFTPSGTTGTTKL TVT ; loop_ _Residue_seq_code _Residue_label 1 GLU 2 GLU 3 CYS 4 GLN 5 VAL 6 ARG 7 VAL 8 GLY 9 ASP 10 LEU 11 THR 12 VAL 13 ALA 14 LYS 15 THR 16 ARG 17 GLY 18 GLN 19 LEU 20 THR 21 ASP 22 ALA 23 ALA 24 PRO 25 ILE 26 GLY 27 PRO 28 VAL 29 THR 30 VAL 31 GLN 32 ALA 33 LEU 34 GLY 35 CYS 36 ASN 37 ALA 38 ARG 39 GLN 40 VAL 41 ALA 42 LEU 43 LYS 44 ALA 45 ASP 46 THR 47 ASP 48 ASN 49 PHE 50 GLU 51 GLN 52 GLY 53 LYS 54 PHE 55 PHE 56 LEU 57 ILE 58 SER 59 ASP 60 ASN 61 ASN 62 ARG 63 ASP 64 LYS 65 LEU 66 TYR 67 VAL 68 ASN 69 ILE 70 ARG 71 PRO 72 MET 73 ASP 74 ASN 75 SER 76 ALA 77 TRP 78 THR 79 THR 80 ASP 81 ASN 82 GLY 83 VAL 84 PHE 85 TYR 86 LYS 87 ASN 88 ASP 89 VAL 90 GLY 91 SER 92 TRP 93 GLY 94 GLY 95 THR 96 ILE 97 GLY 98 ILE 99 TYR 100 VAL 101 ASP 102 GLY 103 GLN 104 GLN 105 THR 106 ASN 107 THR 108 PRO 109 PRO 110 GLY 111 ASN 112 TYR 113 THR 114 LEU 115 THR 116 LEU 117 THR 118 GLY 119 GLY 120 TYR 121 TRP 122 ALA 123 LYS 124 ASP 125 ASN 126 LYS 127 GLN 128 GLY 129 PHE 130 THR 131 PRO 132 SER 133 GLY 134 THR 135 THR 136 GLY 137 THR 138 THR 139 LYS 140 LEU 141 THR 142 VAL 143 THR stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-10-07 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1RXL "Solution Structure Of The Engineered Protein Afae-Dsc" 100.00 156 100.00 100.00 1.27e-98 PDB 1USZ "Semet Afae-3 Adhesin From Escherichia Coli" 86.01 149 99.19 99.19 1.54e-83 PDB 1UT2 "Afae-3 Adhesin From Escherichia Coli" 86.01 149 100.00 100.00 1.17e-84 PDB 2IXQ "The Solution Structure Of The Invasive Tip Complex From Afa-Dr Fibrils" 100.00 143 100.00 100.00 4.86e-98 PDB 2VER "Structural Model For The Complex Between The Dr Adhesins And Carcinoembryonic Antigen (Cea)" 100.00 143 100.00 100.00 4.86e-98 EMBL CAA48847 "AFA-III adhesin [Escherichia coli]" 86.01 160 100.00 100.00 4.21e-85 EMBL CAA54121 "afaE-3 [Escherichia coli]" 86.01 160 100.00 100.00 4.21e-85 GB AAA23709 "Dr hemagglutinin structural subunit [Escherichia coli]" 86.01 160 97.56 98.37 1.94e-82 GB AAK16480 "DraE [Escherichia coli]" 86.01 160 97.56 98.37 1.94e-82 GB ABD93399 "Dr adhesin [Escherichia coli]" 86.01 160 97.56 98.37 1.44e-82 GB ABD93405 "Dr adhesin [Escherichia coli]" 86.01 160 97.56 98.37 4.63e-82 GB ABD93406 "Dr adhesin [Escherichia coli]" 86.01 160 97.56 98.37 1.94e-82 REF WP_021517429 "Dr hemagglutinin structural subunit [Escherichia coli]" 86.01 160 98.37 99.19 1.05e-83 REF WP_021523428 "afimbrial adhesin AFA-I [Escherichia coli]" 86.01 160 97.56 99.19 2.26e-83 REF WP_021566292 "Dr hemagglutinin structural subunit [Escherichia coli]" 86.01 160 98.37 99.19 3.65e-83 SP P24093 "RecName: Full=Dr hemagglutinin structural subunit; Flags: Precursor" 86.01 160 97.56 98.37 1.94e-82 SP Q57254 "RecName: Full=Afimbrial adhesin AFA-III; Flags: Precursor" 86.01 160 100.00 100.00 4.21e-85 stop_ save_ save_DAF1234 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common DAF1234 _Molecular_mass 70000 _Mol_thiol_state 'not reported' _Details . _Residue_count 254 _Mol_residue_sequence ; MQDCGLPPDVPNAQPALEGR TSFPEDTVITYKCEESFVKI PGEKDSVICLKGSQWSDIEE FCNRSCEVPTRLNSASLKQP YITQNYFPVGTVVEYECRPG YRREPSLSPKLTCLQNLKWS TAVEFCKKKSCPNPGEIRNG QIDVPGGILFGATISFSCNT GYKLFGSTSSFCLISGSSVQ WSDPLPECREIYCPAPPQID NGIIQGERDHYGYRQSVTYA CNKGFTMIGEHSIYCTVNND EGEWSGPPPECRGC ; loop_ _Residue_seq_code _Residue_label 1 MET 2 GLN 3 ASP 4 CYS 5 GLY 6 LEU 7 PRO 8 PRO 9 ASP 10 VAL 11 PRO 12 ASN 13 ALA 14 GLN 15 PRO 16 ALA 17 LEU 18 GLU 19 GLY 20 ARG 21 THR 22 SER 23 PHE 24 PRO 25 GLU 26 ASP 27 THR 28 VAL 29 ILE 30 THR 31 TYR 32 LYS 33 CYS 34 GLU 35 GLU 36 SER 37 PHE 38 VAL 39 LYS 40 ILE 41 PRO 42 GLY 43 GLU 44 LYS 45 ASP 46 SER 47 VAL 48 ILE 49 CYS 50 LEU 51 LYS 52 GLY 53 SER 54 GLN 55 TRP 56 SER 57 ASP 58 ILE 59 GLU 60 GLU 61 PHE 62 CYS 63 ASN 64 ARG 65 SER 66 CYS 67 GLU 68 VAL 69 PRO 70 THR 71 ARG 72 LEU 73 ASN 74 SER 75 ALA 76 SER 77 LEU 78 LYS 79 GLN 80 PRO 81 TYR 82 ILE 83 THR 84 GLN 85 ASN 86 TYR 87 PHE 88 PRO 89 VAL 90 GLY 91 THR 92 VAL 93 VAL 94 GLU 95 TYR 96 GLU 97 CYS 98 ARG 99 PRO 100 GLY 101 TYR 102 ARG 103 ARG 104 GLU 105 PRO 106 SER 107 LEU 108 SER 109 PRO 110 LYS 111 LEU 112 THR 113 CYS 114 LEU 115 GLN 116 ASN 117 LEU 118 LYS 119 TRP 120 SER 121 THR 122 ALA 123 VAL 124 GLU 125 PHE 126 CYS 127 LYS 128 LYS 129 LYS 130 SER 131 CYS 132 PRO 133 ASN 134 PRO 135 GLY 136 GLU 137 ILE 138 ARG 139 ASN 140 GLY 141 GLN 142 ILE 143 ASP 144 VAL 145 PRO 146 GLY 147 GLY 148 ILE 149 LEU 150 PHE 151 GLY 152 ALA 153 THR 154 ILE 155 SER 156 PHE 157 SER 158 CYS 159 ASN 160 THR 161 GLY 162 TYR 163 LYS 164 LEU 165 PHE 166 GLY 167 SER 168 THR 169 SER 170 SER 171 PHE 172 CYS 173 LEU 174 ILE 175 SER 176 GLY 177 SER 178 SER 179 VAL 180 GLN 181 TRP 182 SER 183 ASP 184 PRO 185 LEU 186 PRO 187 GLU 188 CYS 189 ARG 190 GLU 191 ILE 192 TYR 193 CYS 194 PRO 195 ALA 196 PRO 197 PRO 198 GLN 199 ILE 200 ASP 201 ASN 202 GLY 203 ILE 204 ILE 205 GLN 206 GLY 207 GLU 208 ARG 209 ASP 210 HIS 211 TYR 212 GLY 213 TYR 214 ARG 215 GLN 216 SER 217 VAL 218 THR 219 TYR 220 ALA 221 CYS 222 ASN 223 LYS 224 GLY 225 PHE 226 THR 227 MET 228 ILE 229 GLY 230 GLU 231 HIS 232 SER 233 ILE 234 TYR 235 CYS 236 THR 237 VAL 238 ASN 239 ASN 240 ASP 241 GLU 242 GLY 243 GLU 244 TRP 245 SER 246 GLY 247 PRO 248 PRO 249 PRO 250 GLU 251 CYS 252 ARG 253 GLY 254 CYS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-30 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1OJV "Decay Accelerating Factor (Cd55): The Structure Of An Intact Human Complement Regulator." 100.00 254 100.00 100.00 0.00e+00 PDB 1OJW "Decay Accelerating Factor (Cd55): The Structure Of An Intact Human Complement Regulator." 100.00 254 100.00 100.00 0.00e+00 PDB 1OJY "Decay Accelerating Factor (Cd55): The Structure Of An Intact Human Complement Regulator." 100.00 254 100.00 100.00 0.00e+00 PDB 1OK1 "Decay Accelerating Factor (cd55): The Structure Of An Intact Human Complement Regulator." 100.00 254 100.00 100.00 0.00e+00 PDB 1OK2 "Decay Accelerating Factor (Cd55): The Structure Of An Intact Human Complement Regulator." 100.00 254 100.00 100.00 0.00e+00 PDB 1OK3 "Decay Accelerating Factor (cd55): The Structure Of An Intact Human Complement Regulator" 99.61 254 99.60 99.60 0.00e+00 PDB 1OK9 "Decay Accelerating Factor (Cd55): The Structure Of An Intact Human Complement Regulator." 100.00 254 100.00 100.00 0.00e+00 PDB 2C8I "Complex Of Echovirus Type 12 With Domains 1, 2, 3 And 4 Of Its Receptor Decay Accelerating Factor (Cd55) By Cryo Electron Micro" 99.21 316 100.00 100.00 0.00e+00 PDB 3IYP "The Interaction Of Decay-Accelerating Factor With Echovirus 7" 99.21 381 100.00 100.00 0.00e+00 PDB 3J24 "Cryoem Reconstruction Of Complement Decay-Accelerating Factor" 100.00 254 100.00 100.00 0.00e+00 DBJ BAE97422 "decay-accelerating factor splicing variant 1 [Homo sapiens]" 98.82 439 100.00 100.00 0.00e+00 DBJ BAE97423 "decay-accelerating factor splicing variant 2 [Homo sapiens]" 98.82 364 100.00 100.00 0.00e+00 DBJ BAE97424 "decay-accelerating factor splicing variant 3 [Homo sapiens]" 98.82 384 100.00 100.00 0.00e+00 DBJ BAE97425 "decay-accelerating factor splicing variant 4 [Homo sapiens]" 98.82 525 100.00 100.00 0.00e+00 DBJ BAE97426 "decay-accelerating factor splicing variant 5 [Homo sapiens]" 98.82 551 100.00 100.00 0.00e+00 GB AAA52167 "decay-accelerating factor precursor, partial [Homo sapiens]" 98.82 376 99.20 99.20 0.00e+00 GB AAA52168 "decay-accelerating factor A [Homo sapiens]" 98.82 381 100.00 100.00 0.00e+00 GB AAA52169 "decay-accelerating factor [Homo sapiens]" 98.82 381 100.00 100.00 0.00e+00 GB AAB48622 "decay-acceleration factor [Homo sapiens]" 98.82 347 99.60 100.00 0.00e+00 GB AAF73175 "decay-accelerating factor [Gorilla gorilla]" 98.82 347 97.61 98.41 0.00e+00 PRF 1303335A "decay accelerating factor long" 98.82 440 100.00 100.00 0.00e+00 PRF 1303335B "decay accelerating factor short" 98.82 381 100.00 100.00 0.00e+00 REF NP_000565 "complement decay-accelerating factor isoform 1 preproprotein [Homo sapiens]" 98.82 381 100.00 100.00 0.00e+00 REF NP_001108224 "complement decay-accelerating factor isoform 2 precursor [Homo sapiens]" 98.82 440 100.00 100.00 0.00e+00 REF NP_001287831 "complement decay-accelerating factor isoform 5 precursor [Homo sapiens]" 98.82 444 100.00 100.00 0.00e+00 REF NP_001287832 "complement decay-accelerating factor isoform 6 precursor [Homo sapiens]" 98.82 439 100.00 100.00 0.00e+00 REF NP_001287833 "complement decay-accelerating factor isoform 7 precursor [Homo sapiens]" 98.82 384 100.00 100.00 0.00e+00 SP P08174 "RecName: Full=Complement decay-accelerating factor; AltName: CD_antigen=CD55; Flags: Precursor [Homo sapiens]" 98.82 381 100.00 100.00 0.00e+00 stop_ save_ save_DAF _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common DAF _Molecular_mass 57794.34 _Mol_thiol_state 'not reported' _Details . _Residue_count 254 _Mol_residue_sequence ; MQDCGLPPDVPNAQPALEGR TSFPEDTVITYKCEESFVKI PGEKDSVICLKGSQWSDIEE FCNRSCEVPTRLNSASLKQP YITQNYFPVGTVVEYECRPG YRREPSLSPKLTCLQNLKWS TAVEFCKKKSCPNPGEIRNG QIDVPGGILFGATISFSCNT GYKLFGSTSSFCLISGSSVQ WSDPLPECREIYCPAPPQID NGIIQGERDHYGYRQSVTYA CNKGFTMIGEHSIYCTVNND EGEWSGPPPECRGC ; loop_ _Residue_seq_code _Residue_label 1 MET 2 GLN 3 ASP 4 CYS 5 GLY 6 LEU 7 PRO 8 PRO 9 ASP 10 VAL 11 PRO 12 ASN 13 ALA 14 GLN 15 PRO 16 ALA 17 LEU 18 GLU 19 GLY 20 ARG 21 THR 22 SER 23 PHE 24 PRO 25 GLU 26 ASP 27 THR 28 VAL 29 ILE 30 THR 31 TYR 32 LYS 33 CYS 34 GLU 35 GLU 36 SER 37 PHE 38 VAL 39 LYS 40 ILE 41 PRO 42 GLY 43 GLU 44 LYS 45 ASP 46 SER 47 VAL 48 ILE 49 CYS 50 LEU 51 LYS 52 GLY 53 SER 54 GLN 55 TRP 56 SER 57 ASP 58 ILE 59 GLU 60 GLU 61 PHE 62 CYS 63 ASN 64 ARG 65 SER 66 CYS 67 GLU 68 VAL 69 PRO 70 THR 71 ARG 72 LEU 73 ASN 74 SER 75 ALA 76 SER 77 LEU 78 LYS 79 GLN 80 PRO 81 TYR 82 ILE 83 THR 84 GLN 85 ASN 86 TYR 87 PHE 88 PRO 89 VAL 90 GLY 91 THR 92 VAL 93 VAL 94 GLU 95 TYR 96 GLU 97 CYS 98 ARG 99 PRO 100 GLY 101 TYR 102 ARG 103 ARG 104 GLU 105 PRO 106 SER 107 LEU 108 SER 109 PRO 110 LYS 111 LEU 112 THR 113 CYS 114 LEU 115 GLN 116 ASN 117 LEU 118 LYS 119 TRP 120 SER 121 THR 122 ALA 123 VAL 124 GLU 125 PHE 126 CYS 127 LYS 128 LYS 129 LYS 130 SER 131 CYS 132 PRO 133 ASN 134 PRO 135 GLY 136 GLU 137 ILE 138 ARG 139 ASN 140 GLY 141 GLN 142 ILE 143 ASP 144 VAL 145 PRO 146 GLY 147 GLY 148 ILE 149 LEU 150 PHE 151 GLY 152 ALA 153 THR 154 ILE 155 SER 156 PHE 157 SER 158 CYS 159 ASN 160 THR 161 GLY 162 TYR 163 LYS 164 LEU 165 PHE 166 GLY 167 SER 168 THR 169 SER 170 SER 171 PHE 172 CYS 173 LEU 174 ILE 175 SER 176 GLY 177 SER 178 SER 179 VAL 180 GLN 181 TRP 182 SER 183 ASP 184 PRO 185 LEU 186 PRO 187 GLU 188 CYS 189 ARG 190 GLU 191 ILE 192 TYR 193 CYS 194 PRO 195 ALA 196 PRO 197 PRO 198 GLN 199 ILE 200 ASP 201 ASN 202 GLY 203 ILE 204 ILE 205 GLN 206 GLY 207 GLU 208 ARG 209 ASP 210 HIS 211 TYR 212 GLY 213 TYR 214 ARG 215 GLN 216 SER 217 VAL 218 THR 219 TYR 220 ALA 221 CYS 222 ASN 223 LYS 224 GLY 225 PHE 226 THR 227 MET 228 ILE 229 GLY 230 GLU 231 HIS 232 SER 233 ILE 234 TYR 235 CYS 236 THR 237 VAL 238 ASN 239 ASN 240 ASP 241 GLU 242 GLY 243 GLU 244 TRP 245 SER 246 GLY 247 PRO 248 PRO 249 PRO 250 GLU 251 CYS 252 ARG 253 GLY 254 CYS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-30 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1OJV "Decay Accelerating Factor (Cd55): The Structure Of An Intact Human Complement Regulator." 100.00 254 100.00 100.00 0.00e+00 PDB 1OJW "Decay Accelerating Factor (Cd55): The Structure Of An Intact Human Complement Regulator." 100.00 254 100.00 100.00 0.00e+00 PDB 1OJY "Decay Accelerating Factor (Cd55): The Structure Of An Intact Human Complement Regulator." 100.00 254 100.00 100.00 0.00e+00 PDB 1OK1 "Decay Accelerating Factor (cd55): The Structure Of An Intact Human Complement Regulator." 100.00 254 100.00 100.00 0.00e+00 PDB 1OK2 "Decay Accelerating Factor (Cd55): The Structure Of An Intact Human Complement Regulator." 100.00 254 100.00 100.00 0.00e+00 PDB 1OK3 "Decay Accelerating Factor (cd55): The Structure Of An Intact Human Complement Regulator" 99.61 254 99.60 99.60 0.00e+00 PDB 1OK9 "Decay Accelerating Factor (Cd55): The Structure Of An Intact Human Complement Regulator." 100.00 254 100.00 100.00 0.00e+00 PDB 2C8I "Complex Of Echovirus Type 12 With Domains 1, 2, 3 And 4 Of Its Receptor Decay Accelerating Factor (Cd55) By Cryo Electron Micro" 99.21 316 100.00 100.00 0.00e+00 PDB 3IYP "The Interaction Of Decay-Accelerating Factor With Echovirus 7" 99.21 381 100.00 100.00 0.00e+00 PDB 3J24 "Cryoem Reconstruction Of Complement Decay-Accelerating Factor" 100.00 254 100.00 100.00 0.00e+00 DBJ BAE97422 "decay-accelerating factor splicing variant 1 [Homo sapiens]" 98.82 439 100.00 100.00 0.00e+00 DBJ BAE97423 "decay-accelerating factor splicing variant 2 [Homo sapiens]" 98.82 364 100.00 100.00 0.00e+00 DBJ BAE97424 "decay-accelerating factor splicing variant 3 [Homo sapiens]" 98.82 384 100.00 100.00 0.00e+00 DBJ BAE97425 "decay-accelerating factor splicing variant 4 [Homo sapiens]" 98.82 525 100.00 100.00 0.00e+00 DBJ BAE97426 "decay-accelerating factor splicing variant 5 [Homo sapiens]" 98.82 551 100.00 100.00 0.00e+00 GB AAA52167 "decay-accelerating factor precursor, partial [Homo sapiens]" 98.82 376 99.20 99.20 0.00e+00 GB AAA52168 "decay-accelerating factor A [Homo sapiens]" 98.82 381 100.00 100.00 0.00e+00 GB AAA52169 "decay-accelerating factor [Homo sapiens]" 98.82 381 100.00 100.00 0.00e+00 GB AAB48622 "decay-acceleration factor [Homo sapiens]" 98.82 347 99.60 100.00 0.00e+00 GB AAF73175 "decay-accelerating factor [Gorilla gorilla]" 98.82 347 97.61 98.41 0.00e+00 PRF 1303335A "decay accelerating factor long" 98.82 440 100.00 100.00 0.00e+00 PRF 1303335B "decay accelerating factor short" 98.82 381 100.00 100.00 0.00e+00 REF NP_000565 "complement decay-accelerating factor isoform 1 preproprotein [Homo sapiens]" 98.82 381 100.00 100.00 0.00e+00 REF NP_001108224 "complement decay-accelerating factor isoform 2 precursor [Homo sapiens]" 98.82 440 100.00 100.00 0.00e+00 REF NP_001287831 "complement decay-accelerating factor isoform 5 precursor [Homo sapiens]" 98.82 444 100.00 100.00 0.00e+00 REF NP_001287832 "complement decay-accelerating factor isoform 6 precursor [Homo sapiens]" 98.82 439 100.00 100.00 0.00e+00 REF NP_001287833 "complement decay-accelerating factor isoform 7 precursor [Homo sapiens]" 98.82 384 100.00 100.00 0.00e+00 SP P08174 "RecName: Full=Complement decay-accelerating factor; AltName: CD_antigen=CD55; Flags: Precursor [Homo sapiens]" 98.82 381 100.00 100.00 0.00e+00 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain _Variant $AfaE-dsc 'E. coli' 562 Eubacteria . Escherichia coli M15 Quiagen stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Variant _Vector_name $AfaE-dsc 'recombinant technology' . Escherichia coli BL21 DE3 na stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $AfaE-dsc 30 uM . . '[U-13C; U-15N]' 'sodium acetate' 50 mM . . 'natural abundance' $DAF . uM 0 30 [U-15N] stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $AfaE-dsc 30 uM . . 'natural abundance' 'sodium acetate' 50 mM . . 'natural abundance' $DAF . uM 0 30 [U-15N] stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $AfaE-dsc 30 uM . . '[U-13C; U-15N]' 'sodium acetate' 50 mM . . 'natural abundance' $DAF . uM 0 30 [U-15N] stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $AfaE-dsc 30 uM . . 'natural abundance' 'sodium acetate' 50 mM . . 'natural abundance' $DAF1234 . uM 0 30 [U-15N] stop_ save_ ############################ # Computer software used # ############################ save_ARIA _Saveframe_category software _Name ARIA _Version . loop_ _Vendor _Address _Electronic_address 'Linge, O'Donoghue and Nilges' . . stop_ loop_ _Task 'data analysis' 'structure solution' stop_ _Details . save_ save_TALOS _Saveframe_category software _Name TALOS _Version . loop_ _Vendor _Address _Electronic_address 'Cornilescu, Delaglio and Bax' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 800 _Details 'NMR service at NIMR' save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HNCO_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HNCO' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_2 save_ save_2D_1H-15N_HNCO_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HNCO' _Sample_label $sample_3 save_ save_2D_1H-15N_HSQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_4 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details 'AfaE-III and AfaE-drsc' loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.3 . pH pressure 1 . atm temperature 298 . K stop_ save_