# 1 "/opt/topspin2.1.3/exp/stan/nmr/lists/pp/hsqcetgpsi" ;hsqcetgpsi ;avance-version (07/04/04) ;HSQC ;2D H-1/X correlation via double inept transfer ; using sensitivity improvement ;phase sensitive using Echo/Antiecho-TPPI gradient selection ;with decoupling during acquisition ;using trim pulses in inept transfer ; ;A.G. Palmer III, J. Cavanagh, P.E. Wright & M. Rance, J. Magn. ; Reson. 93, 151-170 (1991) ;L.E. Kay, P. Keifer & T. Saarinen, J. Am. Chem. Soc. 114, ; 10663-5 (1992) ;J. Schleucher, M. Schwendinger, M. Sattler, P. Schmidt, O. Schedletzky, ; S.J. Glaser, O.W. Sorensen & C. Griesinger, J. Biomol. NMR 4, ; 301-306 (1994) ; ;$CLASS=HighRes ;$DIM=2D ;$TYPE= ;$SUBTYPE= ;$COMMENT= # 1 "/opt/topspin2.1.3/exp/stan/nmr/lists/pp/Avance.incl" 1 ;Avance3.incl ; for AV III ; ;avance-version (07/12/14) ; ;$CLASS=HighRes Incl ;$COMMENT= ;$Id: pulseprogram,v 1.1 2021/02/10 22:35:36 bmrbsvc Exp $ # 26 "/opt/topspin2.1.3/exp/stan/nmr/lists/pp/hsqcetgpsi" 2 # 1 "/opt/topspin2.1.3/exp/stan/nmr/lists/pp/Grad.incl" 1 ;Grad2.incl - include file for Gradient Spectroscopy ; for 1 ; ;avance-version (07/01/17) ; ;$CLASS=HighRes Incl ;$COMMENT= define list EA= ;$Id: pulseprogram,v 1.1 2021/02/10 22:35:36 bmrbsvc Exp $ # 27 "/opt/topspin2.1.3/exp/stan/nmr/lists/pp/hsqcetgpsi" 2 # 1 "/opt/topspin2.1.3/exp/stan/nmr/lists/pp/Delay.incl" 1 ;Delay.incl - include file for commonly used delays ; ;version 00/02/07 ; ;$CLASS=HighRes Incl ;$COMMENT= ;general delays define delay DELTA define delay DELTA1 define delay DELTA2 define delay DELTA3 define delay DELTA4 define delay DELTA5 define delay DELTA6 define delay DELTA7 define delay DELTA8 define delay TAU define delay TAU1 define delay TAU2 define delay TAU3 define delay TAU4 define delay TAU5 ;delays for centering pulses define delay CEN_HN1 define delay CEN_HN2 define delay CEN_HN3 define delay CEN_HC1 define delay CEN_HC2 define delay CEN_HC3 define delay CEN_HC4 define delay CEN_HP1 define delay CEN_HP2 define delay CEN_CN1 define delay CEN_CN2 define delay CEN_CN3 define delay CEN_CN4 define delay CEN_CP1 define delay CEN_CP2 ;loop counters define loopcounter COUNTER define loopcounter SCALEF define loopcounter FACTOR1 define loopcounter FACTOR2 define loopcounter FACTOR3 ;$Id: pulseprogram,v 1.1 2021/02/10 22:35:36 bmrbsvc Exp $ # 28 "/opt/topspin2.1.3/exp/stan/nmr/lists/pp/hsqcetgpsi" 2 "p2=p1*2" "p4=p3*2" "d4=1s/(cnst2*4)" "d11=30m" "d13=4u" "d0=3u" "in0=inf1/2" "DELTA1=d13+p16+d16+4u" "DELTA=p16+d16+50u+p2+d0*2" # 1 "mc_line 57 file /opt/topspin2.1.3/exp/stan/nmr/lists/pp/hsqcetgpsi expanding definition part of mc command before ze" define delay MCWRK define delay MCREST define loopcounter ST1CNT "ST1CNT = td1 / (2)" "MCWRK = 0.142857*d1" "MCREST = d1 - d1" dccorr # 57 "/opt/topspin2.1.3/exp/stan/nmr/lists/pp/hsqcetgpsi" 1 ze # 1 "mc_line 57 file /opt/topspin2.1.3/exp/stan/nmr/lists/pp/hsqcetgpsi expanding definition of mc command after ze" # 58 "/opt/topspin2.1.3/exp/stan/nmr/lists/pp/hsqcetgpsi" d11 pl12:f2 # 1 "mc_line 59 file /opt/topspin2.1.3/exp/stan/nmr/lists/pp/hsqcetgpsi expanding start label for mc command" 2 MCWRK * 2 do:f2 LBLSTS1, MCWRK * 4 LBLF1, MCWRK MCREST # 60 "/opt/topspin2.1.3/exp/stan/nmr/lists/pp/hsqcetgpsi" 3 (p1 ph1) d4 pl2:f2 (center (p2 ph1) (p4 ph6):f2 ) d4 p28 ph1 d13 (p1 ph2) (p3 ph3):f2 d0 (p2 ph7) d0 50u setnmr3|0 setnmr0|34|32|33 ctrlgrad 0 p16:gp1*EA d16 (p4 ph4):f2 DELTA (center (p1 ph1) (p3 ph4):f2 ) d24 (center (p2 ph1) (p4 ph1):f2 ) d24 (center (p1 ph2) (p3 ph5):f2 ) d4 (center (p2 ph1) (p4 ph1):f2 ) d4 (p1 ph1) DELTA1 (p2 ph1) d13 p16:gp2 d16 pl12:f2 4u setnmr3^0 setnmr0^34^32^33 ctrlgrad 7 go=2 ph31 cpd2:f2 # 1 "mc_line 97 file /opt/topspin2.1.3/exp/stan/nmr/lists/pp/hsqcetgpsi expanding mc command in line" MCWRK do:f2 wr #0 if #0 zd igrad EA MCWRK ip5*2 lo to LBLSTS1 times 2 MCWRK id0 MCWRK ip3*2 MCWRK ip6*2 MCWRK ip31*2 lo to LBLF1 times ST1CNT MCWRK # 99 "/opt/topspin2.1.3/exp/stan/nmr/lists/pp/hsqcetgpsi" exit ph1=0 ph2=1 ph3=0 2 ph4=0 0 2 2 ph5=1 1 3 3 ph6=0 ph7=0 0 2 2 ph31=0 2 2 0 ;pl1 : f1 channel - power level for pulse (default) ;pl2 : f2 channel - power level for pulse (default) ;pl3 : f3 channel - power level for pulse (default) ;pl12: f2 channel - power level for CPD/BB decoupling ;p1 : f1 channel - 90 degree high power pulse ;p2 : f1 channel - 180 degree high power pulse ;p3 : f2 channel - 90 degree high power pulse ;p4 : f2 channel - 180 degree high power pulse ;p16: homospoil/gradient pulse ;p22: f3 channel - 180 degree high power pulse ;p28: f1 channel - trim pulse ;d0 : incremented delay (2D) [3 usec] ;d1 : relaxation delay; 1-5 * T1 ;d4 : 1/(4J)XH ;d11: delay for disk I/O [30 msec] ;d13: short delay [4 usec] ;d16: delay for homospoil/gradient recovery ;d24: 1/(8J)XH for all multiplicities ; 1/(4J)XH for XH ;cnst2: = J(XH) ;inf1: 1/SW(X) = 2 * DW(X) ;in0: 1/(2 * SW(X)) = DW(X) ;nd0: 2 ;NS: 1 * n ;DS: >= 16 ;td1: number of experiments ;FnMODE: echo-antiecho ;cpd2: decoupling according to sequence defined by cpdprg2 ;pcpd2: f2 channel - 90 degree pulse for decoupling sequence ;use gradient ratio: gp 1 : gp 2 ; 80 : 20.1 for C-13 ; 80 : 8.1 for N-15 ;for z-only gradients: ;gpz1: 80% ;gpz2: 20.1% for C-13, 8.1% for N-15 ;use gradient files: ;gpnam1: SINE.100 ;gpnam2: SINE.100 ;preprocessor-flags-start ;LABEL_CN: for C-13 and N-15 labeled samples start experiment with ; option -DLABEL_CN (eda: ZGOPTNS) ;preprocessor-flags-end ;$Id: pulseprogram,v 1.1 2021/02/10 22:35:36 bmrbsvc Exp $