data_19995 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; LysRS Anticodon Binding Domian 72-207 ; _BMRB_accession_number 19995 _BMRB_flat_file_name bmr19995.str _Entry_type original _Submission_date 2014-05-30 _Accession_date 2014-05-30 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'LysRS Anticodon Binding Domian 72-207 assigned chemical shifts' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Mushtaq Ameeq Ul . 2 Cho Hye Young . 3 Jeon Young Ho . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 102 "13C chemical shifts" 317 "15N chemical shifts" 102 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2017-07-05 original BMRB . stop_ loop_ _Related_BMRB_accession_number _Relationship 19993 'Lysyl t-RNA synthetase 1-72' stop_ _Original_release_date 2014-05-30 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Characterization of the interaction between lysyl-tRNA synthetase and laminin receptor by NMR ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 24983501 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Cho 'Hye Young' Y. . 2 'Ul Mushtaq' Ameeq . . 3 Lee 'Jin Young' Y. . 4 Kim 'Dae Gyu' G. . 5 Seok 'Min Sook' S. . 6 Jang Minseok . . 7 Han Byung-Woo W. . 8 Kim Sunghoon . . 9 Jeon 'Young Ho' H. . stop_ _Journal_abbreviation 'FEBS Lett.' _Journal_name_full 'FEBS letters' _Journal_volume 588 _Journal_issue 17 _Journal_ISSN 1873-3468 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 2851 _Page_last 2858 _Year 2014 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'LysRS Anticodon Binding Domain 72-207' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'LysRS ABD 72-207' $LysRS_ABD_72-207 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_LysRS_ABD_72-207 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common LysRS_ABD_72-207 _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 136 _Mol_residue_sequence ; DPNQYYKIRSQAIHQLKVNG EDPYPHKFHVDISLTDFIQK YSHLQPGDHLTDITLKVAGR IHAKRASGGKLIFYDLRGEG VKLQVMANSRNYKSEEEFIH INNKLRRGDIIGVQGNPGKT KKGELSIIPYEITLLS ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 72 ASP 2 73 PRO 3 74 ASN 4 75 GLN 5 76 TYR 6 77 TYR 7 78 LYS 8 79 ILE 9 80 ARG 10 81 SER 11 82 GLN 12 83 ALA 13 84 ILE 14 85 HIS 15 86 GLN 16 87 LEU 17 88 LYS 18 89 VAL 19 90 ASN 20 91 GLY 21 92 GLU 22 93 ASP 23 94 PRO 24 95 TYR 25 96 PRO 26 97 HIS 27 98 LYS 28 99 PHE 29 100 HIS 30 101 VAL 31 102 ASP 32 103 ILE 33 104 SER 34 105 LEU 35 106 THR 36 107 ASP 37 108 PHE 38 109 ILE 39 110 GLN 40 111 LYS 41 112 TYR 42 113 SER 43 114 HIS 44 115 LEU 45 116 GLN 46 117 PRO 47 118 GLY 48 119 ASP 49 120 HIS 50 121 LEU 51 122 THR 52 123 ASP 53 124 ILE 54 125 THR 55 126 LEU 56 127 LYS 57 128 VAL 58 129 ALA 59 130 GLY 60 131 ARG 61 132 ILE 62 133 HIS 63 134 ALA 64 135 LYS 65 136 ARG 66 137 ALA 67 138 SER 68 139 GLY 69 140 GLY 70 141 LYS 71 142 LEU 72 143 ILE 73 144 PHE 74 145 TYR 75 146 ASP 76 147 LEU 77 148 ARG 78 149 GLY 79 150 GLU 80 151 GLY 81 152 VAL 82 153 LYS 83 154 LEU 84 155 GLN 85 156 VAL 86 157 MET 87 158 ALA 88 159 ASN 89 160 SER 90 161 ARG 91 162 ASN 92 163 TYR 93 164 LYS 94 165 SER 95 166 GLU 96 167 GLU 97 168 GLU 98 169 PHE 99 170 ILE 100 171 HIS 101 172 ILE 102 173 ASN 103 174 ASN 104 175 LYS 105 176 LEU 106 177 ARG 107 178 ARG 108 179 GLY 109 180 ASP 110 181 ILE 111 182 ILE 112 183 GLY 113 184 VAL 114 185 GLN 115 186 GLY 116 187 ASN 117 188 PRO 118 189 GLY 119 190 LYS 120 191 THR 121 192 LYS 122 193 LYS 123 194 GLY 124 195 GLU 125 196 LEU 126 197 SER 127 198 ILE 128 199 ILE 129 200 PRO 130 201 TYR 131 202 GLU 132 203 ILE 133 204 THR 134 205 LEU 135 206 LEU 136 207 SER stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $LysRS_ABD_72-207 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $LysRS_ABD_72-207 'recombinant technology' . Escherichia coli . pET28a stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $LysRS_ABD_72-207 0.2 mM '[U-100% 13C; U-100% 15N]' 'sodium chloride' 100 mM 'natural abundance' HEPES 20 mM 'natural abundance' PMSF 1 mM 'natural abundance' DTT 1 mM 'natural abundance' D2O 10 % 'natural abundance' H2O 90 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CCPN _Saveframe_category software _Name CCPN _Version 2.1.5 loop_ _Vendor _Address _Electronic_address CCPN . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HNCO_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HCACO_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HCACO' _Sample_label $sample_1 save_ save_3D_HNCA_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details '13C/15N LysRS72-207 in buffer containing 100mM NaCl, 20mM HEPES, 1mM PMSF and ImM DTT.' loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 100 . mM pH 7.0 . pH pressure 1 . atm temperature 303 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0 external indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0 external direct . . . 1 DSS N 15 'methyl protons' ppm 0 external indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D CBCA(CO)NH' '3D HNCACB' '3D HNCO' '3D HCACO' '3D HNCA' '3D HN(CO)CA' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'LysRS ABD 72-207' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 73 2 PRO C C 177.300 . . 2 73 2 PRO CA C 60.400 . . 3 73 2 PRO CB C 32.100 . . 4 74 3 ASN H H 8.411 . . 5 74 3 ASN C C 173.530 . . 6 74 3 ASN CA C 53.910 . . 7 74 3 ASN CB C 38.640 . . 8 74 3 ASN N N 117.590 . . 9 75 4 GLN H H 7.825 . . 10 75 4 GLN C C 176.474 . . 11 75 4 GLN CA C 56.150 . . 12 75 4 GLN N N 119.750 . . 13 83 12 ALA H H 8.109 . . 14 83 12 ALA C C 177.882 . . 15 83 12 ALA CA C 52.700 . . 16 83 12 ALA CB C 19.058 . . 17 83 12 ALA N N 125.250 . . 18 84 13 ILE H H 7.867 . . 19 84 13 ILE C C 176.260 . . 20 84 13 ILE CA C 61.610 . . 21 84 13 ILE CB C 38.560 . . 22 84 13 ILE N N 119.476 . . 23 87 16 LEU C C 177.189 . . 24 87 16 LEU CA C 55.410 . . 25 87 16 LEU CB C 42.404 . . 26 88 17 LYS H H 8.167 . . 27 88 17 LYS C C 176.642 . . 28 88 17 LYS CA C 56.200 . . 29 88 17 LYS CB C 32.980 . . 30 88 17 LYS N N 122.810 . . 31 89 18 VAL H H 8.106 . . 32 89 18 VAL C C 176.050 . . 33 89 18 VAL CA C 62.410 . . 34 89 18 VAL CB C 32.980 . . 35 89 18 VAL N N 121.890 . . 36 90 19 ASN H H 8.459 . . 37 90 19 ASN CA C 53.426 . . 38 90 19 ASN CB C 38.869 . . 39 90 19 ASN N N 122.850 . . 40 91 20 GLY H H 8.235 . . 41 91 20 GLY C C 173.900 . . 42 91 20 GLY CA C 45.447 . . 43 91 20 GLY N N 109.910 . . 44 92 21 GLU H H 8.077 . . 45 92 21 GLU C C 175.970 . . 46 92 21 GLU CA C 56.086 . . 47 92 21 GLU CB C 30.668 . . 48 92 21 GLU N N 120.710 . . 49 93 22 ASP H H 8.229 . . 50 93 22 ASP C C 174.658 . . 51 93 22 ASP CA C 52.320 . . 52 93 22 ASP CB C 41.256 . . 53 93 22 ASP N N 123.480 . . 54 96 25 PRO C C 176.360 . . 55 96 25 PRO CA C 63.320 . . 56 96 25 PRO CB C 31.800 . . 57 101 30 VAL H H 7.934 . . 58 101 30 VAL C C 176.280 . . 59 101 30 VAL CA C 61.417 . . 60 101 30 VAL CB C 32.670 . . 61 101 30 VAL N N 119.054 . . 62 102 31 ASP H H 9.055 . . 63 102 31 ASP C C 175.900 . . 64 102 31 ASP CA C 55.670 . . 65 102 31 ASP CB C 44.140 . . 66 102 31 ASP N N 121.460 . . 67 103 32 ILE H H 7.683 . . 68 103 32 ILE C C 171.922 . . 69 103 32 ILE CA C 59.675 . . 70 103 32 ILE CB C 41.380 . . 71 103 32 ILE N N 118.360 . . 72 104 33 SER H H 8.736 . . 73 104 33 SER C C 175.344 . . 74 104 33 SER CA C 57.280 . . 75 104 33 SER CB C 64.451 . . 76 104 33 SER N N 122.280 . . 77 105 34 LEU H H 9.887 . . 78 105 34 LEU C C 179.604 . . 79 105 34 LEU CA C 58.686 . . 80 105 34 LEU CB C 41.820 . . 81 105 34 LEU N N 124.023 . . 82 106 35 THR H H 8.682 . . 83 106 35 THR C C 177.207 . . 84 106 35 THR CA C 67.322 . . 85 106 35 THR CB C 68.957 . . 86 106 35 THR N N 113.760 . . 87 107 36 ASP H H 8.098 . . 88 107 36 ASP C C 178.261 . . 89 107 36 ASP CA C 57.123 . . 90 107 36 ASP CB C 39.510 . . 91 107 36 ASP N N 122.576 . . 92 108 37 PHE H H 8.713 . . 93 108 37 PHE C C 177.188 . . 94 108 37 PHE CA C 62.249 . . 95 108 37 PHE CB C 39.238 . . 96 108 37 PHE N N 125.052 . . 97 109 38 ILE H H 8.254 . . 98 109 38 ILE C C 178.970 . . 99 109 38 ILE CA C 64.880 . . 100 109 38 ILE CB C 38.494 . . 101 109 38 ILE N N 118.702 . . 102 110 39 GLN H H 7.619 . . 103 110 39 GLN C C 178.723 . . 104 110 39 GLN CA C 58.892 . . 105 110 39 GLN CB C 28.712 . . 106 110 39 GLN N N 119.417 . . 107 111 40 LYS H H 8.362 . . 108 111 40 LYS C C 178.220 . . 109 111 40 LYS CA C 58.946 . . 110 111 40 LYS CB C 33.145 . . 111 111 40 LYS N N 119.470 . . 112 112 41 TYR H H 7.583 . . 113 112 41 TYR C C 175.551 . . 114 112 41 TYR CA C 59.069 . . 115 112 41 TYR CB C 37.942 . . 116 112 41 TYR N N 112.148 . . 117 113 42 SER H H 7.565 . . 118 113 42 SER CA C 62.727 . . 119 113 42 SER CB C 63.380 . . 120 113 42 SER N N 116.320 . . 121 114 43 HIS C C 175.705 . . 122 114 43 HIS CA C 57.115 . . 123 114 43 HIS CB C 29.558 . . 124 115 44 LEU H H 6.875 . . 125 115 44 LEU C C 176.390 . . 126 115 44 LEU CA C 55.562 . . 127 115 44 LEU CB C 42.111 . . 128 115 44 LEU N N 121.309 . . 129 116 45 GLN H H 9.086 . . 130 116 45 GLN CA C 53.332 . . 131 116 45 GLN N N 122.410 . . 132 117 46 PRO C C 177.210 . . 133 117 46 PRO CA C 64.830 . . 134 117 46 PRO CB C 31.656 . . 135 118 47 GLY H H 7.306 . . 136 118 47 GLY C C 174.194 . . 137 118 47 GLY CA C 45.120 . . 138 118 47 GLY N N 115.100 . . 139 119 48 ASP H H 8.042 . . 140 119 48 ASP C C 176.344 . . 141 119 48 ASP CA C 55.455 . . 142 119 48 ASP CB C 42.359 . . 143 119 48 ASP N N 122.574 . . 144 120 49 HIS H H 8.620 . . 145 120 49 HIS C C 174.399 . . 146 120 49 HIS CA C 54.524 . . 147 120 49 HIS CB C 33.229 . . 148 120 49 HIS N N 120.686 . . 149 121 50 LEU H H 8.633 . . 150 121 50 LEU C C 176.131 . . 151 121 50 LEU CA C 53.654 . . 152 121 50 LEU CB C 39.237 . . 153 121 50 LEU N N 128.389 . . 154 122 51 THR H H 7.417 . . 155 122 51 THR C C 174.917 . . 156 122 51 THR CA C 63.669 . . 157 122 51 THR CB C 68.409 . . 158 122 51 THR N N 114.422 . . 159 123 52 ASP H H 8.716 . . 160 123 52 ASP C C 175.162 . . 161 123 52 ASP CA C 53.910 . . 162 123 52 ASP CB C 40.264 . . 163 123 52 ASP N N 118.370 . . 164 124 53 ILE H H 7.227 . . 165 124 53 ILE C C 174.590 . . 166 124 53 ILE CA C 59.240 . . 167 124 53 ILE CB C 38.961 . . 168 124 53 ILE N N 121.866 . . 169 125 54 THR H H 8.412 . . 170 125 54 THR C C 173.435 . . 171 125 54 THR CA C 61.464 . . 172 125 54 THR CB C 69.898 . . 173 125 54 THR N N 124.913 . . 174 126 55 LEU H H 9.218 . . 175 126 55 LEU C C 175.549 . . 176 126 55 LEU CA C 53.461 . . 177 126 55 LEU CB C 47.552 . . 178 126 55 LEU N N 128.690 . . 179 127 56 LYS H H 9.426 . . 180 127 56 LYS C C 176.902 . . 181 127 56 LYS CA C 55.399 . . 182 127 56 LYS CB C 34.387 . . 183 127 56 LYS N N 121.036 . . 184 128 57 VAL H H 8.637 . . 185 128 57 VAL C C 174.057 . . 186 128 57 VAL CA C 58.707 . . 187 128 57 VAL CB C 35.290 . . 188 128 57 VAL N N 114.668 . . 189 129 58 ALA H H 8.565 . . 190 129 58 ALA C C 175.861 . . 191 129 58 ALA CA C 51.302 . . 192 129 58 ALA CB C 23.059 . . 193 129 58 ALA N N 121.500 . . 194 130 59 GLY H H 8.111 . . 195 130 59 GLY C C 182.662 . . 196 130 59 GLY CA C 45.427 . . 197 130 59 GLY N N 103.129 . . 198 131 60 ARG H H 8.702 . . 199 131 60 ARG C C 177.035 . . 200 131 60 ARG CA C 54.224 . . 201 131 60 ARG CB C 33.346 . . 202 131 60 ARG N N 120.439 . . 203 134 63 ALA H H 7.553 . . 204 134 63 ALA C C 174.146 . . 205 134 63 ALA CA C 51.804 . . 206 134 63 ALA CB C 22.908 . . 207 134 63 ALA N N 117.899 . . 208 135 64 LYS H H 8.594 . . 209 135 64 LYS C C 174.401 . . 210 135 64 LYS CA C 54.993 . . 211 135 64 LYS CB C 36.931 . . 212 135 64 LYS N N 123.982 . . 213 136 65 ARG H H 8.912 . . 214 136 65 ARG C C 173.980 . . 215 136 65 ARG CA C 55.120 . . 216 136 65 ARG CB C 33.190 . . 217 136 65 ARG N N 127.408 . . 218 137 66 ALA CA C 52.120 . . 219 137 66 ALA CB C 20.263 . . 220 138 67 SER H H 8.605 . . 221 138 67 SER CA C 57.182 . . 222 138 67 SER CB C 63.829 . . 223 138 67 SER N N 119.682 . . 224 141 70 LYS H H 8.316 . . 225 141 70 LYS C C 174.311 . . 226 141 70 LYS CA C 56.139 . . 227 141 70 LYS CB C 33.030 . . 228 141 70 LYS N N 120.975 . . 229 142 71 LEU H H 6.902 . . 230 142 71 LEU C C 174.027 . . 231 142 71 LEU CA C 54.452 . . 232 142 71 LEU CB C 44.144 . . 233 142 71 LEU N N 121.400 . . 234 143 72 ILE H H 8.798 . . 235 143 72 ILE C C 172.818 . . 236 143 72 ILE CA C 60.297 . . 237 143 72 ILE CB C 41.780 . . 238 143 72 ILE N N 127.508 . . 239 144 73 PHE H H 8.522 . . 240 144 73 PHE C C 176.077 . . 241 144 73 PHE CA C 55.790 . . 242 144 73 PHE CB C 41.486 . . 243 144 73 PHE N N 123.226 . . 244 145 74 TYR H H 9.014 . . 245 145 74 TYR C C 174.538 . . 246 145 74 TYR CA C 56.450 . . 247 145 74 TYR CB C 40.595 . . 248 145 74 TYR N N 119.366 . . 249 146 75 ASP H H 8.330 . . 250 146 75 ASP C C 173.928 . . 251 146 75 ASP CA C 53.473 . . 252 146 75 ASP CB C 42.063 . . 253 146 75 ASP N N 125.126 . . 254 147 76 LEU H H 8.637 . . 255 147 76 LEU C C 175.708 . . 256 147 76 LEU CA C 53.846 . . 257 147 76 LEU CB C 45.661 . . 258 147 76 LEU N N 130.535 . . 259 148 77 ARG H H 9.100 . . 260 148 77 ARG C C 176.350 . . 261 148 77 ARG CA C 54.361 . . 262 148 77 ARG CB C 34.165 . . 263 148 77 ARG N N 121.972 . . 264 149 78 GLY H H 7.688 . . 265 149 78 GLY C C 172.481 . . 266 149 78 GLY CA C 45.744 . . 267 149 78 GLY N N 111.431 . . 268 150 79 GLU H H 8.897 . . 269 150 79 GLU C C 175.816 . . 270 150 79 GLU CA C 57.056 . . 271 150 79 GLU CB C 27.607 . . 272 150 79 GLU N N 116.416 . . 273 151 80 GLY H H 8.463 . . 274 151 80 GLY C C 174.858 . . 275 151 80 GLY CA C 45.616 . . 276 151 80 GLY N N 107.267 . . 277 152 81 VAL H H 8.219 . . 278 152 81 VAL C C 174.044 . . 279 152 81 VAL CA C 59.695 . . 280 152 81 VAL CB C 34.865 . . 281 152 81 VAL N N 116.534 . . 282 153 82 LYS H H 7.705 . . 283 153 82 LYS C C 173.433 . . 284 153 82 LYS CA C 54.293 . . 285 153 82 LYS CB C 36.369 . . 286 153 82 LYS N N 118.430 . . 287 154 83 LEU H H 9.047 . . 288 154 83 LEU C C 174.244 . . 289 154 83 LEU CA C 53.928 . . 290 154 83 LEU CB C 47.183 . . 291 154 83 LEU N N 122.768 . . 292 155 84 GLN H H 7.510 . . 293 155 84 GLN C C 175.209 . . 294 155 84 GLN CA C 55.864 . . 295 155 84 GLN CB C 30.879 . . 296 155 84 GLN N N 124.785 . . 297 156 85 VAL H H 9.604 . . 298 156 85 VAL C C 175.282 . . 299 156 85 VAL CA C 60.447 . . 300 156 85 VAL CB C 32.853 . . 301 156 85 VAL N N 127.225 . . 302 157 86 MET H H 8.388 . . 303 157 86 MET CA C 54.060 . . 304 157 86 MET CB C 34.297 . . 305 157 86 MET N N 130.684 . . 306 158 87 ALA C C 173.562 . . 307 158 87 ALA CA C 51.237 . . 308 158 87 ALA CB C 18.339 . . 309 159 88 ASN H H 8.081 . . 310 159 88 ASN C C 177.210 . . 311 159 88 ASN CA C 51.204 . . 312 159 88 ASN CB C 41.327 . . 313 159 88 ASN N N 124.050 . . 314 160 89 SER H H 8.303 . . 315 160 89 SER C C 175.520 . . 316 160 89 SER CA C 62.183 . . 317 160 89 SER N N 125.835 . . 318 161 90 ARG H H 7.800 . . 319 161 90 ARG C C 176.850 . . 320 161 90 ARG CA C 58.020 . . 321 161 90 ARG CB C 29.870 . . 322 161 90 ARG N N 119.080 . . 323 162 91 ASN H H 7.424 . . 324 162 91 ASN C C 173.746 . . 325 162 91 ASN CA C 53.870 . . 326 162 91 ASN CB C 41.570 . . 327 162 91 ASN N N 114.554 . . 328 163 92 TYR H H 7.131 . . 329 163 92 TYR C C 175.420 . . 330 163 92 TYR CA C 58.900 . . 331 163 92 TYR CB C 40.509 . . 332 163 92 TYR N N 120.758 . . 333 164 93 LYS H H 7.346 . . 334 164 93 LYS C C 176.479 . . 335 164 93 LYS CA C 60.394 . . 336 164 93 LYS CB C 31.691 . . 337 164 93 LYS N N 124.979 . . 338 165 94 SER H H 6.864 . . 339 165 94 SER C C 174.950 . . 340 165 94 SER CA C 58.214 . . 341 165 94 SER CB C 65.804 . . 342 165 94 SER N N 108.770 . . 343 166 95 GLU H H 9.111 . . 344 166 95 GLU C C 178.636 . . 345 166 95 GLU CA C 60.372 . . 346 166 95 GLU CB C 29.618 . . 347 166 95 GLU N N 125.750 . . 348 167 96 GLU H H 8.958 . . 349 167 96 GLU C C 179.721 . . 350 167 96 GLU CA C 60.144 . . 351 167 96 GLU CB C 29.216 . . 352 167 96 GLU N N 120.013 . . 353 168 97 GLU H H 7.856 . . 354 168 97 GLU C C 178.013 . . 355 168 97 GLU CA C 59.274 . . 356 168 97 GLU CB C 29.552 . . 357 168 97 GLU N N 118.161 . . 358 169 98 PHE H H 7.919 . . 359 169 98 PHE C C 177.455 . . 360 169 98 PHE CA C 61.477 . . 361 169 98 PHE CB C 38.158 . . 362 169 98 PHE N N 122.124 . . 363 170 99 ILE H H 7.907 . . 364 170 99 ILE C C 178.049 . . 365 170 99 ILE CA C 64.361 . . 366 170 99 ILE CB C 38.273 . . 367 170 99 ILE N N 121.769 . . 368 171 100 HIS H H 7.364 . . 369 171 100 HIS C C 178.714 . . 370 171 100 HIS CA C 59.451 . . 371 171 100 HIS CB C 30.650 . . 372 171 100 HIS N N 117.512 . . 373 172 101 ILE H H 8.211 . . 374 172 101 ILE C C 177.160 . . 375 172 101 ILE CA C 60.993 . . 376 172 101 ILE CB C 37.361 . . 377 172 101 ILE N N 118.242 . . 378 173 102 ASN H H 7.147 . . 379 173 102 ASN C C 176.483 . . 380 173 102 ASN CA C 56.833 . . 381 173 102 ASN CB C 37.854 . . 382 173 102 ASN N N 119.760 . . 383 174 103 ASN H H 7.463 . . 384 174 103 ASN C C 176.401 . . 385 174 103 ASN CA C 54.749 . . 386 174 103 ASN CB C 38.438 . . 387 174 103 ASN N N 116.784 . . 388 175 104 LYS H H 7.207 . . 389 175 104 LYS C C 177.032 . . 390 175 104 LYS CA C 57.138 . . 391 175 104 LYS CB C 34.064 . . 392 175 104 LYS N N 118.630 . . 393 176 105 LEU H H 6.942 . . 394 176 105 LEU C C 177.219 . . 395 176 105 LEU CA C 55.600 . . 396 176 105 LEU CB C 42.122 . . 397 176 105 LEU N N 118.630 . . 398 177 106 ARG H H 9.099 . . 399 177 106 ARG C C 175.148 . . 400 177 106 ARG CA C 53.528 . . 401 177 106 ARG CB C 34.281 . . 402 177 106 ARG N N 122.516 . . 403 178 107 ARG H H 8.393 . . 404 178 107 ARG C C 176.606 . . 405 178 107 ARG CA C 58.527 . . 406 178 107 ARG CB C 29.773 . . 407 178 107 ARG N N 120.146 . . 408 179 108 GLY H H 9.178 . . 409 179 108 GLY C C 174.239 . . 410 179 108 GLY CA C 44.680 . . 411 179 108 GLY N N 115.910 . . 412 180 109 ASP H H 7.827 . . 413 180 109 ASP C C 174.948 . . 414 180 109 ASP CA C 55.716 . . 415 180 109 ASP CB C 41.549 . . 416 180 109 ASP N N 122.210 . . 417 181 110 ILE H H 8.421 . . 418 181 110 ILE CA C 55.750 . . 419 181 110 ILE N N 122.580 . . 420 182 111 ILE C C 173.400 . . 421 182 111 ILE CA C 58.240 . . 422 182 111 ILE CB C 41.800 . . 423 183 112 GLY H H 8.816 . . 424 183 112 GLY C C 172.933 . . 425 183 112 GLY CA C 43.625 . . 426 183 112 GLY N N 108.060 . . 427 184 113 VAL H H 9.353 . . 428 184 113 VAL C C 173.341 . . 429 184 113 VAL CA C 61.240 . . 430 184 113 VAL CB C 34.430 . . 431 184 113 VAL N N 124.752 . . 432 185 114 GLN H H 8.633 . . 433 185 114 GLN C C 176.862 . . 434 185 114 GLN CA C 53.245 . . 435 185 114 GLN CB C 31.334 . . 436 185 114 GLN N N 127.630 . . 437 186 115 GLY H H 9.621 . . 438 186 115 GLY C C 183.518 . . 439 186 115 GLY CA C 47.017 . . 440 186 115 GLY N N 116.070 . . 441 187 116 ASN H H 8.302 . . 442 187 116 ASN C C 173.320 . . 443 187 116 ASN CA C 50.411 . . 444 187 116 ASN CB C 43.101 . . 445 187 116 ASN N N 116.683 . . 446 188 117 PRO C C 175.270 . . 447 188 117 PRO CA C 62.388 . . 448 188 117 PRO CB C 32.546 . . 449 189 118 GLY H H 8.366 . . 450 189 118 GLY C C 183.618 . . 451 189 118 GLY CA C 45.932 . . 452 189 118 GLY N N 109.290 . . 453 190 119 LYS H H 8.562 . . 454 190 119 LYS C C 177.597 . . 455 190 119 LYS CA C 55.354 . . 456 190 119 LYS CB C 37.245 . . 457 190 119 LYS N N 119.030 . . 458 191 120 THR H H 8.292 . . 459 191 120 THR C C 177.884 . . 460 191 120 THR CA C 61.379 . . 461 191 120 THR CB C 71.543 . . 462 191 120 THR N N 115.265 . . 463 193 122 LYS C C 176.680 . . 464 193 122 LYS CA C 56.394 . . 465 193 122 LYS CB C 32.488 . . 466 194 123 GLY H H 8.005 . . 467 194 123 GLY C C 173.509 . . 468 194 123 GLY CA C 45.329 . . 469 194 123 GLY N N 107.329 . . 470 195 124 GLU H H 7.347 . . 471 195 124 GLU C C 177.160 . . 472 195 124 GLU CA C 55.411 . . 473 195 124 GLU CB C 30.052 . . 474 195 124 GLU N N 119.870 . . 475 196 125 LEU H H 9.300 . . 476 196 125 LEU C C 175.517 . . 477 196 125 LEU CA C 57.041 . . 478 196 125 LEU CB C 41.501 . . 479 196 125 LEU N N 132.827 . . 480 197 126 SER H H 9.278 . . 481 197 126 SER C C 171.369 . . 482 197 126 SER CA C 56.951 . . 483 197 126 SER CB C 68.707 . . 484 197 126 SER N N 121.461 . . 485 198 127 ILE H H 8.457 . . 486 198 127 ILE C C 173.417 . . 487 198 127 ILE CA C 58.232 . . 488 198 127 ILE CB C 42.345 . . 489 198 127 ILE N N 118.350 . . 490 199 128 ILE H H 9.111 . . 491 199 128 ILE C C 176.220 . . 492 199 128 ILE CB C 39.26 . . 493 199 128 ILE N N 132.324 . . 494 200 129 PRO C C 175.512 . . 495 200 129 PRO CA C 63.338 . . 496 200 129 PRO CB C 33.224 . . 497 201 130 TYR H H 9.757 . . 498 201 130 TYR C C 173.718 . . 499 201 130 TYR CA C 59.409 . . 500 201 130 TYR CB C 40.975 . . 501 201 130 TYR N N 124.647 . . 502 202 131 GLU H H 7.539 . . 503 202 131 GLU C C 174.463 . . 504 202 131 GLU CA C 56.929 . . 505 202 131 GLU CB C 34.161 . . 506 202 131 GLU N N 113.453 . . 507 203 132 ILE H H 8.853 . . 508 203 132 ILE C C 173.486 . . 509 203 132 ILE CA C 60.430 . . 510 203 132 ILE CB C 40.776 . . 511 203 132 ILE N N 130.176 . . 512 204 133 THR H H 9.086 . . 513 204 133 THR C C 173.187 . . 514 204 133 THR CA C 61.085 . . 515 204 133 THR CB C 71.359 . . 516 204 133 THR N N 123.667 . . 517 205 134 LEU H H 8.819 . . 518 205 134 LEU C C 173.931 . . 519 205 134 LEU CA C 55.912 . . 520 205 134 LEU CB C 42.467 . . 521 205 134 LEU N N 129.948 . . stop_ save_