data_26667
#######################
# Entry information #
#######################
save_entry_information
_Saveframe_category entry_information
_Entry_title
;
Backbone and side chain order parameters for calcium-bound calmodulin (E84K)
;
_BMRB_accession_number 26667
_BMRB_flat_file_name bmr26667.str
_Entry_type original
_Submission_date 2015-09-21
_Accession_date 2015-09-21
_Entry_origination author
_NMR_STAR_version 2.1.1
_Experimental_method NMR
_Details .
loop_
_Author_ordinal
_Author_family_name
_Author_given_name
_Author_middle_initials
_Author_family_title
1 Harpole Kyle W. .
2 Kasinath Vignesh . .
3 Lim Jackwee . .
4 Valentine Kathleen G. .
5 Sharp Kim A. .
6 Wand Joshua A. .
stop_
loop_
_Saveframe_category_type
_Saveframe_category_type_count
S2_parameters 2
stop_
loop_
_Data_type
_Data_type_count
"order parameters" 152
stop_
loop_
_Revision_date
_Revision_keyword
_Revision_author
_Revision_detail
2017-06-27 original BMRB .
stop_
loop_
_Related_BMRB_accession_number
_Relationship
26665 'Calmodulin (D58N)'
26666 'Calmodulin (D95N)'
26668 'Calmodulin (D58N):smMLCK(p)'
26669 'Calmodulin (D95N):smMLCK(p)'
26670 'Calmodulin (E84K):nNOS(p)'
stop_
_Original_release_date 2015-09-22
save_
#############################
# Citation for this entry #
#############################
save_entry_citation
_Saveframe_category entry_citation
_Citation_full .
_Citation_title
;
Entropy in molecular recognition by proteins
;
_Citation_status published
_Citation_type journal
_CAS_abstract_code .
_MEDLINE_UI_code .
_PubMed_ID 28584100
loop_
_Author_ordinal
_Author_family_name
_Author_given_name
_Author_middle_initials
_Author_family_title
1 Caro Jose A. .
2 Harpole Kyle W. .
3 Kasinath Vignesh . .
4 Lim Jackwee . .
5 Granja Jeffrey . .
6 Valentine Kathleen G. .
7 Sharp Kim A. .
8 Wand 'A Joshua' J. .
stop_
_Journal_abbreviation 'Proc. Natl. Acad. Sci. U.S.A.'
_Journal_name_full 'Proceedings of the National Academy of Sciences of the United States of America'
_Journal_volume 114
_Journal_issue 25
_Journal_ISSN 1091-6490
_Journal_CSD .
_Book_chapter_title .
_Book_volume .
_Book_series .
_Book_ISBN .
_Conference_state_province .
_Conference_abstract_number .
_Page_first 6563
_Page_last 6568
_Year 2017
_Details .
save_
##################################
# Molecular system description #
##################################
save_assembly
_Saveframe_category molecular_system
_Mol_system_name 'Calmodulin (E84K)'
_Enzyme_commission_number .
loop_
_Mol_system_component_name
_Mol_label
'Calmodulin (E84K)' $Calmodulin(E84K)
stop_
_System_molecular_weight .
_System_physical_state native
_System_oligomer_state ?
_System_paramagnetic no
_System_thiol_state .
_Database_query_date .
_Details .
save_
########################
# Monomeric polymers #
########################
save_Calmodulin(E84K)
_Saveframe_category monomeric_polymer
_Mol_type polymer
_Mol_polymer_class protein
_Name_common Calmodulin(E84K)
_Molecular_mass .
_Mol_thiol_state 'not present'
_Details .
##############################
# Polymer residue sequence #
##############################
_Residue_count 148
_Mol_residue_sequence
;
ADQLTEEQIAEFKEAFSLFD
KDGDGTITTKELGTVMRSLG
QNPTEAELQDMINEVDADGN
GTIDFPEFLTMMARKMKDTD
SEEKIREAFRVFDKDGNGYI
SAAELRHVMTNLGEKLTDEE
VDEMIREADIDGDGQVNYEE
FVQMMTAK
;
loop_
_Residue_seq_code
_Residue_label
1 ALA 2 ASP 3 GLN 4 LEU 5 THR
6 GLU 7 GLU 8 GLN 9 ILE 10 ALA
11 GLU 12 PHE 13 LYS 14 GLU 15 ALA
16 PHE 17 SER 18 LEU 19 PHE 20 ASP
21 LYS 22 ASP 23 GLY 24 ASP 25 GLY
26 THR 27 ILE 28 THR 29 THR 30 LYS
31 GLU 32 LEU 33 GLY 34 THR 35 VAL
36 MET 37 ARG 38 SER 39 LEU 40 GLY
41 GLN 42 ASN 43 PRO 44 THR 45 GLU
46 ALA 47 GLU 48 LEU 49 GLN 50 ASP
51 MET 52 ILE 53 ASN 54 GLU 55 VAL
56 ASP 57 ALA 58 ASP 59 GLY 60 ASN
61 GLY 62 THR 63 ILE 64 ASP 65 PHE
66 PRO 67 GLU 68 PHE 69 LEU 70 THR
71 MET 72 MET 73 ALA 74 ARG 75 LYS
76 MET 77 LYS 78 ASP 79 THR 80 ASP
81 SER 82 GLU 83 GLU 84 LYS 85 ILE
86 ARG 87 GLU 88 ALA 89 PHE 90 ARG
91 VAL 92 PHE 93 ASP 94 LYS 95 ASP
96 GLY 97 ASN 98 GLY 99 TYR 100 ILE
101 SER 102 ALA 103 ALA 104 GLU 105 LEU
106 ARG 107 HIS 108 VAL 109 MET 110 THR
111 ASN 112 LEU 113 GLY 114 GLU 115 LYS
116 LEU 117 THR 118 ASP 119 GLU 120 GLU
121 VAL 122 ASP 123 GLU 124 MET 125 ILE
126 ARG 127 GLU 128 ALA 129 ASP 130 ILE
131 ASP 132 GLY 133 ASP 134 GLY 135 GLN
136 VAL 137 ASN 138 TYR 139 GLU 140 GLU
141 PHE 142 VAL 143 GLN 144 MET 145 MET
146 THR 147 ALA 148 LYS
stop_
_Sequence_homology_query_date .
_Sequence_homology_query_revised_last_date .
save_
####################
# Natural source #
####################
save_natural_source
_Saveframe_category natural_source
loop_
_Mol_label
_Organism_name_common
_NCBI_taxonomy_ID
_Superkingdom
_Kingdom
_Genus
_Species
$Calmodulin(E84K) chicken 9031 Eukaryota Metazoa Gallus gallus
stop_
save_
#########################
# Experimental source #
#########################
save_experimental_source
_Saveframe_category experimental_source
loop_
_Mol_label
_Production_method
_Host_organism_name_common
_Genus
_Species
_Strain
_Vector_name
$Calmodulin(E84K) 'recombinant technology' . Escherichia coli . pET15b
stop_
save_
#####################################
# Sample contents and methodology #
#####################################
########################
# Sample description #
########################
save_Rlx_sample
_Saveframe_category sample
_Sample_type solution
_Details .
loop_
_Mol_label
_Concentration_value
_Concentration_value_units
_Isotopic_labeling
$Calmodulin(E84K) 0.4 mM '[U-99% 15N]'
$Calmodulin(E84K) 1 mM '[U-13C; U-2H]'
stop_
save_
############################
# Computer software used #
############################
save_Felix
_Saveframe_category software
_Name Felix
_Version .
loop_
_Vendor
_Address
_Electronic_address
'Accelrys Software Inc.' . .
stop_
loop_
_Task
'data analysis'
stop_
_Details .
save_
#########################
# Experimental detail #
#########################
##################################
# NMR Spectrometer definitions #
##################################
save_spectrometer_1
_Saveframe_category NMR_spectrometer
_Manufacturer Bruker
_Model Avance
_Field_strength 500
_Details .
save_
save_spectrometer_2
_Saveframe_category NMR_spectrometer
_Manufacturer Bruker
_Model Avance
_Field_strength 600
_Details .
save_
save_spectrometer_3
_Saveframe_category NMR_spectrometer
_Manufacturer Bruker
_Model Avance
_Field_strength 750
_Details .
save_
#############################
# NMR applied experiments #
#############################
save_15N_T1,T2,NOE_1
_Saveframe_category NMR_applied_experiment
_Experiment_name '15N T1,T2,NOE'
_Sample_label $Rlx_sample
save_
save_2H_IzCz-compensated_Dz_and_Dy_2
_Saveframe_category NMR_applied_experiment
_Experiment_name '2H IzCz-compensated Dz and Dy'
_Sample_label $Rlx_sample
save_
#######################
# Sample conditions #
#######################
save_sample_conditions_1
_Saveframe_category sample_conditions
_Details .
loop_
_Variable_type
_Variable_value
_Variable_value_error
_Variable_value_units
pH 6.5 . pH
pressure 1 . atm
temperature 308 . K
stop_
save_
save_Methyl_Order_parameters
_Saveframe_category S2_parameters
_Details .
loop_
_Sample_label
$Rlx_sample
stop_
_Sample_conditions_label $sample_conditions_1
_Mol_system_component_name 'Calmodulin (E84K)'
_Tau_e_value_units .
_Tau_s_value_units .
_Text_data_format .
_Text_data .
loop_
_Residue_seq_code
_Residue_label
_Atom_name
_Model_fit
_S2_value
_S2_value_fit_error
_Tau_e_value
_Tau_e_value_fit_error
_S2f_value
_S2f_value_fit_error
_S2s_value
_S2s_value_fit_error
_Tau_s_value
_Tau_s_value_fit_error
_S2H_value
_S2H_value_fit_error
_S2N_value
_S2N_value_fit_error
4 LEU CD1 . 0.446 0.013 5.08E-11 1.31E-12 . . . . . . . . . .
4 LEU CD2 . 0.403 0.009 3.41E-11 6.82E-13 . . . . . . . . . .
5 THR CG2 . 0.768 0.005 3.54E-11 5.43E-13 . . . . . . . . . .
9 ILE CG2 . 0.831 0.006 2.82E-11 7.24E-13 . . . . . . . . . .
9 ILE CD1 . 0.397 0.005 1.86E-11 4.95E-13 . . . . . . . . . .
10 ALA CB . 0.909 0.008 3.40E-11 6.49E-13 . . . . . . . . . .
15 ALA CB . 1.072 0.011 6.36E-11 1.69E-12 . . . . . . . . . .
18 LEU CD1 . 0.227 0.004 3.93E-11 7.31E-13 . . . . . . . . . .
18 LEU CD2 . 0.287 0.003 3.97E-11 5.18E-13 . . . . . . . . . .
26 THR CG2 . 0.562 0.017 8.94E-11 1.12E-12 . . . . . . . . . .
27 ILE CG2 . 0.272 0.028 2.97E-11 5.29E-13 . . . . . . . . . .
29 THR CG2 . 0.419 0.007 6.71E-11 7.55E-13 . . . . . . . . . .
32 LEU CD1 . 0.526 0.014 3.04E-11 6.61E-13 . . . . . . . . . .
35 VAL CG2 . 0.775 0.011 2.47E-11 7.37E-13 . . . . . . . . . .
36 MET CE . 0.269 0.004 9.90E-12 3.06E-13 . . . . . . . . . .
48 LEU CD2 . 0.390 0.006 2.99E-11 4.32E-13 . . . . . . . . . .
51 MET CE . 0.224 0.003 9.00E-12 0.01E-13 . . . . . . . . . .
52 ILE CG2 . 0.623 0.007 4.11E-11 7.66E-13 . . . . . . . . . .
52 ILE CD1 . 0.361 0.005 1.86E-11 5.35E-13 . . . . . . . . . .
55 VAL CG1 . 0.611 0.007 5.07E-11 8.74E-13 . . . . . . . . . .
55 VAL CG2 . 0.574 0.010 4.39E-11 1.08E-12 . . . . . . . . . .
62 THR CG2 . 0.865 0.022 1.03E-10 2.92E-12 . . . . . . . . . .
63 ILE CD1 . 0.443 0.004 3.05E-11 7.66E-13 . . . . . . . . . .
63 ILE CG2 . 0.792 0.012 2.51E-11 1.15E-12 . . . . . . . . . .
69 LEU CD1 . 0.444 0.010 3.85E-11 1.60E-12 . . . . . . . . . .
69 LEU CD2 . 0.456 0.032 4.24E-11 3.35E-12 . . . . . . . . . .
70 THR CG2 . 0.661 0.006 4.60E-11 5.11E-13 . . . . . . . . . .
71 MET CE . 0.171 0.004 9.47E-12 5.00E-13 . . . . . . . . . .
72 MET CE . 0.280 0.001 1.00E-11 1.09E-13 . . . . . . . . . .
73 ALA CB . 0.868 0.005 4.32E-11 4.66E-13 . . . . . . . . . .
76 MET CE . 0.111 0.011 8.70E-12 4.59E-13 . . . . . . . . . .
79 THR CG2 . 0.354 0.007 5.17E-11 4.77E-13 . . . . . . . . . .
85 ILE CG2 . 0.624 0.006 2.97E-11 8.54E-13 . . . . . . . . . .
85 ILE CD1 . 0.386 0.003 2.38E-11 5.02E-13 . . . . . . . . . .
88 ALA CB . 0.942 0.021 6.92E-11 2.86E-12 . . . . . . . . . .
91 VAL CG1 . 0.818 0.007 4.72E-11 9.77E-13 . . . . . . . . . .
91 VAL CG2 . 0.747 0.006 2.75E-11 1.07E-12 . . . . . . . . . .
100 ILE CG2 . 0.841 0.011 3.48E-11 5.69E-13 . . . . . . . . . .
102 ALA CB . 1.000 0.008 3.79E-11 6.31E-13 . . . . . . . . . .
105 LEU CD1 . 0.395 0.006 3.16E-11 7.08E-13 . . . . . . . . . .
105 LEU CD2 . 0.273 0.015 3.50E-11 1.19E-12 . . . . . . . . . .
108 VAL CG2 . 0.560 0.004 3.10E-11 9.75E-13 . . . . . . . . . .
109 MET CE . 0.216 0.004 8.00E-12 0.01E-13 . . . . . . . . . .
112 LEU CD1 . 0.481 0.024 5.13E-11 1.61E-12 . . . . . . . . . .
116 LEU CD1 . 0.391 0.022 5.71E-11 2.70E-12 . . . . . . . . . .
116 LEU CD2 . 0.329 0.006 3.54E-11 5.10E-13 . . . . . . . . . .
121 VAL CG1 . 0.599 0.006 4.13E-11 4.73E-13 . . . . . . . . . .
121 VAL CG2 . 0.593 0.006 3.66E-11 6.10E-13 . . . . . . . . . .
124 MET CE . 0.230 0.002 8.00E-12 0.01E-13 . . . . . . . . . .
125 ILE CG2 . 0.684 0.006 3.65E-11 5.96E-13 . . . . . . . . . .
125 ILE CD1 . 0.241 0.003 2.78E-11 4.58E-13 . . . . . . . . . .
128 ALA CB . 0.878 0.013 6.02E-11 1.07E-12 . . . . . . . . . .
130 ILE CG2 . 0.572 0.003 3.79E-11 3.63E-13 . . . . . . . . . .
130 ILE CD1 . 0.369 0.004 2.51E-11 2.59E-13 . . . . . . . . . .
136 VAL CG2 . 0.795 0.007 4.83E-11 8.36E-13 . . . . . . . . . .
142 VAL CG1 . 0.789 0.008 5.19E-11 1.21E-12 . . . . . . . . . .
142 VAL CG2 . 0.702 0.007 2.46E-11 5.77E-13 . . . . . . . . . .
145 MET CE . 0.224 0.009 7.53E-12 5.00E-13 . . . . . . . . . .
146 THR CG2 . 0.467 0.007 5.10E-11 5.98E-13 . . . . . . . . . .
147 ALA CB . 0.370 0.002 3.71E-11 2.84E-23 . . . . . . . . . .
stop_
save_
save_Backbone_Order_Parameters
_Saveframe_category S2_parameters
_Details .
loop_
_Sample_label
$Rlx_sample
stop_
_Sample_conditions_label $sample_conditions_1
_Mol_system_component_name 'Calmodulin (E84K)'
_Tau_e_value_units .
_Tau_s_value_units .
_Text_data_format .
_Text_data .
loop_
_Residue_seq_code
_Residue_label
_Atom_name
_Model_fit
_S2_value
_S2_value_fit_error
_Tau_e_value
_Tau_e_value_fit_error
_S2f_value
_S2f_value_fit_error
_S2s_value
_S2s_value_fit_error
_Tau_s_value
_Tau_s_value_fit_error
_S2H_value
_S2H_value_fit_error
_S2N_value
_S2N_value_fit_error
6 GLU N . 0.901 0.003 5.06E-09 4.96E-10 . . . . . . . . . .
7 GLU N . 0.914 0.005 7.65E-09 5.95E-10 . . . . . . . . . .
9 ILE N . 0.938 0.002 2.87E-09 3.39E-10 . . . . . . . . . .
10 ALA N . 0.949 0.001 4.27E-09 1.33E-10 . . . . . . . . . .
12 PHE N . 0.954 0.002 3.79E-09 5.56E-11 . . . . . . . . . .
13 LYS N . 0.954 0.002 4.34E-09 2.95E-10 . . . . . . . . . .
14 GLU N . 0.933 0.001 4.63E-09 1.26E-10 . . . . . . . . . .
15 ALA N . 0.961 0.001 4.13E-09 1.28E-10 . . . . . . . . . .
16 PHE N . 0.977 0.002 3.97E-09 1.11E-10 . . . . . . . . . .
17 SER N . 0.964 0.002 3.78E-09 1.44E-10 . . . . . . . . . .
18 LEU N . 0.962 0.001 4.29E-09 8.61E-11 . . . . . . . . . .
20 ASP N . 0.936 0.001 4.06E-09 8.36E-11 . . . . . . . . . .
21 LYS N . 0.906 0.005 1.30E-09 1.02E-10 . . . . . . . . . .
22 ASP N . 0.904 0.005 8.16E-09 5.08E-10 . . . . . . . . . .
24 ASP N . 0.948 0.003 8.80E-09 5.08E-10 . . . . . . . . . .
26 THR N . 0.943 0.001 4.67E-09 2.02E-10 . . . . . . . . . .
27 ILE N . 0.955 0.003 2.60E-09 4.11E-10 . . . . . . . . . .
28 THR N . 0.951 0.003 4.60E-09 4.59E-10 . . . . . . . . . .
29 THR N . 0.918 0.003 9.20E-09 0.00E+00 . . . . . . . . . .
30 LYS N . 0.925 0.004 8.53E-09 6.21E-10 . . . . . . . . . .
31 GLU N . 0.925 0.004 4.48E-09 3.30E-09 . . . . . . . . . .
32 LEU N . 0.948 0.003 5.51E-09 4.84E-10 . . . . . . . . . .
33 GLY N . 0.912 0.003 9.20E-09 2.93E-11 . . . . . . . . . .
35 VAL N . 0.923 0.002 9.20E-09 0.00E+00 . . . . . . . . . .
37 ARG N . 0.924 0.004 7.56E-09 5.25E-10 . . . . . . . . . .
39 LEU N . 0.919 0.003 4.91E-09 3.79E-10 . . . . . . . . . .
41 GLN N . 0.925 0.002 2.78E-09 4.16E-10 . . . . . . . . . .
42 ASN N . 0.782 0.006 1.01E-09 4.94E-11 . . . . . . . . . .
45 GLU N . 0.923 0.002 9.20E-09 0.00E+00 . . . . . . . . . .
46 ALA N . 0.908 0.003 8.22E-09 3.28E-10 . . . . . . . . . .
48 LEU N . 0.910 0.003 9.07E-09 2.44E-10 . . . . . . . . . .
52 ILE N . 0.929 0.003 7.47E-09 5.16E-10 . . . . . . . . . .
54 GLU N . 0.930 0.004 6.83E-09 1.07E-09 . . . . . . . . . .
56 ASP N . 0.926 0.004 4.35E-09 3.44E-09 . . . . . . . . . .
57 ALA N . 0.746 0.003 4.84E-10 1.23E-11 . . . . . . . . . .
58 ASP N . 0.894 0.003 9.05E-09 2.52E-10 . . . . . . . . . .
61 GLY N . 0.926 0.003 8.35E-09 4.69E-10 . . . . . . . . . .
63 ILE N . 0.961 0.002 3.16E-09 5.34E-10 . . . . . . . . . .
64 ASP N . 0.944 0.004 4.51E-09 1.27E-09 . . . . . . . . . .
65 PHE N . 0.952 0.002 3.85E-09 1.61E-10 . . . . . . . . . .
68 PHE N . 0.941 0.005 8.39E-09 9.10E-10 . . . . . . . . . .
69 LEU N . 0.948 0.004 8.86E-09 5.81E-10 . . . . . . . . . .
70 THR N . 0.898 0.006 1.70E-09 2.03E-10 . . . . . . . . . .
71 MET N . 0.943 0.002 4.32E-09 1.10E-10 . . . . . . . . . .
72 MET N . 0.946 0.002 4.30E-09 1.38E-10 . . . . . . . . . .
73 ALA N . 0.931 0.002 4.32E-09 1.70E-10 . . . . . . . . . .
74 ARG N . 0.909 0.002 5.41E-09 2.69E-10 . . . . . . . . . .
77 LYS N . 0.761 0.007 6.59E-09 2.95E-10 . . . . . . . . . .
78 ASP N . 0.633 0.022 7.62E-09 3.18E-09 . . . . . . . . . .
79 THR N . 0.635 0.001 9.20E-09 0.00E+00 . . . . . . . . . .
80 ASP N . 0.691 0.002 9.15E-09 6.36E-10 . . . . . . . . . .
81 SER N . 0.767 0.006 8.92E-10 3.56E-11 . . . . . . . . . .
82 GLU N . 0.802 0.002 9.20E-09 0.00E+00 . . . . . . . . . .
83 GLU N . 0.865 0.002 3.86E-09 3.87E-10 . . . . . . . . . .
86 ARG N . 0.953 0.002 3.39E-09 4.44E-11 . . . . . . . . . .
89 PHE N . 0.848 0.005 7.32E-10 3.66E-11 . . . . . . . . . .
90 ARG N . 0.834 0.004 5.07E-10 2.39E-11 . . . . . . . . . .
91 VAL N . 0.969 0.002 3.09E-09 1.18E-10 . . . . . . . . . .
93 ASP N . 0.951 0.001 3.00E-09 8.37E-11 . . . . . . . . . .
95 ASP N . 0.903 0.002 9.10E-09 1.19E-11 . . . . . . . . . .
96 GLY N . 0.918 0.005 8.29E-09 6.01E-10 . . . . . . . . . .
99 TYR N . 0.939 0.004 7.01E-09 1.56E-09 . . . . . . . . . .
100 ILE N . 0.886 0.004 1.03E-09 5.88E-11 . . . . . . . . . .
101 SER N . 0.951 0.004 5.20E-09 1.11E-09 . . . . . . . . . .
102 ALA N . 0.930 0.004 1.11E-09 8.53E-11 . . . . . . . . . .
105 LEU N . 0.945 0.003 4.60E-09 6.34E-10 . . . . . . . . . .
109 MET N . 0.926 0.001 3.79E-09 2.58E-11 . . . . . . . . . .
110 THR N . 0.902 0.002 4.17E-09 2.32E-10 . . . . . . . . . .
114 GLU N . 0.856 0.009 5.79E-09 2.26E-09 . . . . . . . . . .
115 LYS N . 0.704 0.011 2.69E-09 9.18E-10 . . . . . . . . . .
116 LEU N . 0.614 0.004 8.60E-10 1.80E-11 . . . . . . . . . .
117 THR N . 0.814 0.004 1.01E-09 3.16E-11 . . . . . . . . . .
118 ASP N . 0.926 0.003 2.07E-09 1.81E-10 . . . . . . . . . .
119 GLU N . 0.921 0.002 4.48E-09 3.74E-10 . . . . . . . . . .
121 VAL N . 0.928 0.001 3.69E-09 3.88E-11 . . . . . . . . . .
125 ILE N . 0.942 0.001 3.59E-09 4.54E-11 . . . . . . . . . .
127 GLU N . 0.965 0.001 3.89E-09 2.01E-11 . . . . . . . . . .
128 ALA N . 0.936 0.002 3.67E-09 3.96E-11 . . . . . . . . . .
129 ASP N . 0.950 0.001 3.61E-09 3.45E-11 . . . . . . . . . .
130 ILE N . 0.656 0.003 3.53E-10 7.57E-12 . . . . . . . . . .
131 ASP N . 0.905 0.004 7.87E-09 4.70E-10 . . . . . . . . . .
133 ASP N . 0.898 0.002 6.62E-10 2.46E-11 . . . . . . . . . .
134 GLY N . 0.932 0.004 4.89E-09 1.94E-09 . . . . . . . . . .
135 GLN N . 0.942 0.002 5.12E-09 4.57E-10 . . . . . . . . . .
136 VAL N . 0.928 0.004 1.66E-09 1.96E-10 . . . . . . . . . .
137 ASN N . 0.929 0.006 6.01E-09 1.78E-09 . . . . . . . . . .
140 GLU N . 0.927 0.003 7.11E-09 6.00E-10 . . . . . . . . . .
141 PHE N . 0.911 0.003 9.10E-09 4.35E-11 . . . . . . . . . .
142 VAL N . 0.937 0.001 4.23E-09 8.47E-11 . . . . . . . . . .
143 GLN N . 0.924 0.002 5.18E-09 1.91E-10 . . . . . . . . . .
145 MET N . 0.922 0.002 3.94E-09 3.59E-11 . . . . . . . . . .
147 ALA N . 0.743 0.003 5.66E-09 1.60E-10 . . . . . . . . . .
stop_
save_