data_27438 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Competitive Regulation of the Cold Sensing Ion Channel TRPM8 by PIP2 and PIRT ; _BMRB_accession_number 27438 _BMRB_flat_file_name bmr27438.str _Entry_type original _Submission_date 2018-03-29 _Accession_date 2018-03-29 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Sisco Nicholas J. . 2 Helsel Cole VM. . 3 Rath Parthasarathi . . 4 Machorro Dorothy . . 5 'Van Horn' Wade D. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 92 "13C chemical shifts" 141 "15N chemical shifts" 91 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2019-04-12 original BMRB . stop_ _Original_release_date 2018-04-02 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Phosphoinositide-interacting regulator of TRP (PIRT) has opposing effects on human and mouse TRPM8 ion channels ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 29724821 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Hilton Jacob K. . 2 Salehpour Taraneh . . 3 Sisco Nicholas J. . 4 Rath Parthasarathi . . 5 'Van Horn' Wade D. . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_name_full 'The Journal of biological chemistry' _Journal_volume 293 _Journal_issue 24 _Journal_ISSN 1083-351X _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 9423 _Page_last 9434 _Year 2018 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Human PIRT' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Human PIRT' $Human_PIRT stop_ _System_molecular_weight 16660 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Human_PIRT _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Human_PIRT _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 146 _Mol_residue_sequence ; MGHHHHHHGMTMETLPKVLE VDEKSPEAKDLLPSQTASSL CISSRSESVWTTTPRSNWEI YRKPIVIMSVGGAILLFGVV ITCLAYTLKLSDKSLSILKM VGPGFLSLGLMMLVCGLVWV PIIKKKQKHRQKSNFLRSLK SFFLTR ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 -8 MET 2 -7 GLY 3 -6 HIS 4 -5 HIS 5 -4 HIS 6 -3 HIS 7 -2 HIS 8 -1 HIS 9 0 GLY 10 1 MET 11 2 THR 12 3 MET 13 4 GLU 14 5 THR 15 6 LEU 16 7 PRO 17 8 LYS 18 9 VAL 19 10 LEU 20 11 GLU 21 12 VAL 22 13 ASP 23 14 GLU 24 15 LYS 25 16 SER 26 17 PRO 27 18 GLU 28 19 ALA 29 20 LYS 30 21 ASP 31 22 LEU 32 23 LEU 33 24 PRO 34 25 SER 35 26 GLN 36 27 THR 37 28 ALA 38 29 SER 39 30 SER 40 31 LEU 41 32 CYS 42 33 ILE 43 34 SER 44 35 SER 45 36 ARG 46 37 SER 47 38 GLU 48 39 SER 49 40 VAL 50 41 TRP 51 42 THR 52 43 THR 53 44 THR 54 45 PRO 55 46 ARG 56 47 SER 57 48 ASN 58 49 TRP 59 50 GLU 60 51 ILE 61 52 TYR 62 53 ARG 63 54 LYS 64 55 PRO 65 56 ILE 66 57 VAL 67 58 ILE 68 59 MET 69 60 SER 70 61 VAL 71 62 GLY 72 63 GLY 73 64 ALA 74 65 ILE 75 66 LEU 76 67 LEU 77 68 PHE 78 69 GLY 79 70 VAL 80 71 VAL 81 72 ILE 82 73 THR 83 74 CYS 84 75 LEU 85 76 ALA 86 77 TYR 87 78 THR 88 79 LEU 89 80 LYS 90 81 LEU 91 82 SER 92 83 ASP 93 84 LYS 94 85 SER 95 86 LEU 96 87 SER 97 88 ILE 98 89 LEU 99 90 LYS 100 91 MET 101 92 VAL 102 93 GLY 103 94 PRO 104 95 GLY 105 96 PHE 106 97 LEU 107 98 SER 108 99 LEU 109 100 GLY 110 101 LEU 111 102 MET 112 103 MET 113 104 LEU 114 105 VAL 115 106 CYS 116 107 GLY 117 108 LEU 118 109 VAL 119 110 TRP 120 111 VAL 121 112 PRO 122 113 ILE 123 114 ILE 124 115 LYS 125 116 LYS 126 117 LYS 127 118 GLN 128 119 LYS 129 120 HIS 130 121 ARG 131 122 GLN 132 123 LYS 133 124 SER 134 125 ASN 135 126 PHE 136 127 LEU 137 128 ARG 138 129 SER 139 130 LEU 140 131 LYS 141 132 SER 142 133 PHE 143 134 PHE 144 135 LEU 145 136 THR 146 137 ARG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Human_PIRT Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Human_PIRT 'recombinant technology' . Escherichia coli BL21(START)DE3 pET16b stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Human_PIRT 500-750 uM '[U-100% 13C; U-100% 15N]' stop_ save_ ############################ # Computer software used # ############################ save_CCPN _Saveframe_category software _Name CCPN _Version . loop_ _Vendor _Address _Electronic_address CCPN . . stop_ loop_ _Task 'chemical shift assignment' 'data analysis' 'peak picking' stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 850 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ save_3D_HN(COCA)CB_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(COCA)CB' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.5 . pH pressure 1 . atm temperature 273 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCA' '3D HNCACB' '3D HN(CO)CA' '3D HN(COCA)CB' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'Human PIRT' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 10 MET H H 7.748 . . 2 1 10 MET CA C 57.089 . . 3 1 10 MET CB C 33.932 . . 4 1 10 MET N N 125.587 . . 5 2 11 THR H H 7.714 . . 6 2 11 THR CA C 61.748 . . 7 2 11 THR N N 113.846 . . 8 3 12 MET H H 8.088 . . 9 3 12 MET CA C 52.891 . . 10 3 12 MET CB C 41.714 . . 11 3 12 MET N N 127.440 . . 12 4 13 GLU H H 8.097 . . 13 4 13 GLU CA C 56.617 . . 14 4 13 GLU N N 120.225 . . 15 5 14 THR H H 8.006 . . 16 5 14 THR CA C 61.753 . . 17 5 14 THR CB C 69.934 . . 18 5 14 THR N N 115.967 . . 19 6 15 LEU H H 7.960 . . 20 6 15 LEU CA C 53.661 . . 21 6 15 LEU CB C 41.241 . . 22 6 15 LEU N N 125.863 . . 23 18 27 GLU H H 7.943 . . 24 18 27 GLU CA C 62.094 . . 25 18 27 GLU CB C 33.093 . . 26 18 27 GLU N N 120.431 . . 27 19 28 ALA H H 8.102 . . 28 19 28 ALA CA C 52.963 . . 29 19 28 ALA CB C 19.018 . . 30 19 28 ALA N N 125.442 . . 31 20 29 LYS H H 8.034 . . 32 20 29 LYS CA C 56.708 . . 33 20 29 LYS CB C 32.991 . . 34 20 29 LYS N N 120.029 . . 35 21 30 ASP H H 8.034 . . 36 21 30 ASP CA C 54.607 . . 37 21 30 ASP CB C 41.164 . . 38 21 30 ASP N N 120.485 . . 39 22 31 LEU H H 7.846 . . 40 22 31 LEU CA C 55.057 . . 41 22 31 LEU CB C 42.579 . . 42 22 31 LEU N N 121.776 . . 43 23 32 LEU H H 7.969 . . 44 23 32 LEU CA C 53.180 . . 45 23 32 LEU CB C 41.904 . . 46 23 32 LEU N N 124.031 . . 47 24 33 PRO CA C 62.634 . . 48 25 34 SER H H 8.469 . . 49 25 34 SER CA C 58.589 . . 50 25 34 SER CB C 63.975 . . 51 25 34 SER N N 116.744 . . 52 26 35 GLN H H 7.930 . . 53 26 35 GLN CA C 54.927 . . 54 26 35 GLN CB C 40.369 . . 55 26 35 GLN N N 126.209 . . 56 27 36 THR H H 7.964 . . 57 27 36 THR CA C 62.302 . . 58 27 36 THR CB C 69.824 . . 59 27 36 THR N N 115.057 . . 60 28 37 ALA H H 8.152 . . 61 28 37 ALA CA C 54.988 . . 62 28 37 ALA N N 126.451 . . 63 29 38 SER H H 7.960 . . 64 29 38 SER CA C 58.038 . . 65 29 38 SER CB C 63.736 . . 66 29 38 SER N N 123.231 . . 67 30 39 SER H H 7.857 . . 68 30 39 SER CA C 59.595 . . 69 30 39 SER N N 119.035 . . 70 32 41 CYS H H 8.304 . . 71 32 41 CYS CA C 56.673 . . 72 32 41 CYS CB C 30.471 . . 73 32 41 CYS N N 122.464 . . 74 33 42 ILE H H 8.182 . . 75 33 42 ILE CA C 56.064 . . 76 33 42 ILE CB C 32.978 . . 77 33 42 ILE N N 122.243 . . 78 34 43 SER H H 8.159 . . 79 34 43 SER CA C 56.532 . . 80 34 43 SER CB C 63.391 . . 81 34 43 SER N N 118.767 . . 82 35 44 SER H H 8.390 . . 83 35 44 SER CA C 56.632 . . 84 35 44 SER CB C 63.319 . . 85 35 44 SER N N 120.305 . . 86 42 51 THR H H 8.046 . . 87 42 51 THR CA C 61.798 . . 88 42 51 THR CB C 69.940 . . 89 42 51 THR N N 115.625 . . 90 43 52 THR H H 7.905 . . 91 43 52 THR CA C 61.796 . . 92 43 52 THR CB C 69.838 . . 93 43 52 THR N N 115.499 . . 94 57 66 VAL H H 7.975 . . 95 57 66 VAL CA C 67.291 . . 96 57 66 VAL N N 121.166 . . 97 58 67 ILE H H 7.999 . . 98 58 67 ILE CA C 61.983 . . 99 58 67 ILE N N 119.336 . . 100 59 68 MET H H 7.828 . . 101 59 68 MET CA C 59.086 . . 102 59 68 MET N N 117.016 . . 103 60 69 SER H H 7.133 . . 104 60 69 SER CA C 56.729 . . 105 60 69 SER CB C 64.555 . . 106 60 69 SER N N 113.733 . . 107 61 70 VAL H H 8.316 . . 108 61 70 VAL CA C 65.597 . . 109 61 70 VAL CB C 39.577 . . 110 61 70 VAL N N 116.781 . . 111 62 71 GLY H H 8.478 . . 112 62 71 GLY CA C 49.262 . . 113 62 71 GLY N N 106.388 . . 114 63 72 GLY H H 8.480 . . 115 63 72 GLY CA C 49.261 . . 116 63 72 GLY N N 107.645 . . 117 64 73 ALA H H 7.947 . . 118 64 73 ALA CA C 54.659 . . 119 64 73 ALA CB C 18.570 . . 120 64 73 ALA N N 123.746 . . 121 65 74 ILE H H 8.625 . . 122 65 74 ILE CA C 66.052 . . 123 65 74 ILE N N 122.119 . . 124 66 75 LEU H H 7.928 . . 125 66 75 LEU CA C 62.848 . . 126 66 75 LEU N N 113.626 . . 127 67 76 LEU H H 7.873 . . 128 67 76 LEU CA C 61.064 . . 129 67 76 LEU N N 119.383 . . 130 68 77 PHE H H 7.838 . . 131 68 77 PHE CA C 60.835 . . 132 68 77 PHE N N 123.356 . . 133 69 78 GLY H H 8.978 . . 134 69 78 GLY CA C 48.468 . . 135 69 78 GLY N N 106.979 . . 136 70 79 VAL H H 8.853 . . 137 70 79 VAL CA C 67.195 . . 138 70 79 VAL N N 126.524 . . 139 71 80 VAL H H 8.215 . . 140 71 80 VAL CA C 67.198 . . 141 71 80 VAL N N 119.941 . . 142 72 81 ILE H H 8.570 . . 143 72 81 ILE CA C 64.773 . . 144 72 81 ILE N N 117.448 . . 145 73 82 THR H H 8.077 . . 146 73 82 THR CA C 68.265 . . 147 73 82 THR N N 116.714 . . 148 74 83 CYS H H 8.210 . . 149 74 83 CYS CA C 64.141 . . 150 74 83 CYS N N 117.804 . . 151 75 84 LEU H H 7.928 . . 152 75 84 LEU CA C 57.518 . . 153 75 84 LEU CB C 42.094 . . 154 75 84 LEU N N 122.772 . . 155 76 85 ALA H H 8.232 . . 156 76 85 ALA CA C 53.870 . . 157 76 85 ALA CB C 17.255 . . 158 76 85 ALA N N 120.491 . . 159 77 86 TYR H H 7.230 . . 160 77 86 TYR CA C 59.275 . . 161 77 86 TYR CB C 39.288 . . 162 77 86 TYR N N 111.155 . . 163 78 87 THR H H 7.543 . . 164 78 87 THR CA C 63.139 . . 165 78 87 THR CB C 70.560 . . 166 78 87 THR N N 110.350 . . 167 79 88 LEU H H 7.931 . . 168 79 88 LEU CA C 54.320 . . 169 79 88 LEU CB C 42.045 . . 170 79 88 LEU N N 121.358 . . 171 80 89 LYS H H 8.585 . . 172 80 89 LYS CA C 55.244 . . 173 80 89 LYS CB C 29.599 . . 174 80 89 LYS N N 123.223 . . 175 81 90 LEU H H 6.900 . . 176 81 90 LEU CA C 52.702 . . 177 81 90 LEU CB C 43.268 . . 178 81 90 LEU N N 122.417 . . 179 82 91 SER H H 8.655 . . 180 82 91 SER CA C 58.116 . . 181 82 91 SER CB C 63.775 . . 182 82 91 SER N N 115.833 . . 183 86 95 LEU H H 7.695 . . 184 86 95 LEU CA C 52.627 . . 185 86 95 LEU CB C 43.029 . . 186 86 95 LEU N N 121.916 . . 187 87 96 SER H H 7.779 . . 188 87 96 SER CA C 59.976 . . 189 87 96 SER CB C 65.057 . . 190 87 96 SER N N 121.565 . . 191 88 97 ILE H H 7.991 . . 192 88 97 ILE CA C 62.051 . . 193 88 97 ILE N N 116.848 . . 194 89 98 LEU H H 7.910 . . 195 89 98 LEU CA C 52.489 . . 196 89 98 LEU CB C 43.454 . . 197 89 98 LEU N N 124.540 . . 198 93 102 GLY H H 8.441 . . 199 93 102 GLY CA C 48.578 . . 200 93 102 GLY N N 107.361 . . 201 94 103 PRO CA C 64.443 . . 202 95 104 GLY H H 7.892 . . 203 95 104 GLY CA C 47.247 . . 204 95 104 GLY N N 107.240 . . 205 96 105 PHE H H 8.634 . . 206 96 105 PHE CA C 60.536 . . 207 96 105 PHE N N 122.156 . . 208 97 106 LEU H H 8.489 . . 209 97 106 LEU CA C 59.067 . . 210 97 106 LEU N N 119.754 . . 211 98 107 SER H H 7.993 . . 212 98 107 SER CA C 62.695 . . 213 98 107 SER N N 113.632 . . 214 99 108 LEU H H 8.092 . . 215 99 108 LEU CA C 57.605 . . 216 99 108 LEU N N 122.772 . . 217 100 109 GLY H H 8.698 . . 218 100 109 GLY CA C 47.954 . . 219 100 109 GLY N N 106.992 . . 220 101 110 LEU H H 8.103 . . 221 101 110 LEU CA C 57.856 . . 222 101 110 LEU N N 121.048 . . 223 102 111 MET H H 8.393 . . 224 102 111 MET CA C 58.402 . . 225 102 111 MET N N 121.426 . . 226 103 112 MET H H 7.967 . . 227 103 112 MET CA C 58.355 . . 228 103 112 MET N N 120.942 . . 229 104 113 LEU H H 7.581 . . 230 104 113 LEU CA C 63.117 . . 231 104 113 LEU N N 117.099 . . 232 105 114 VAL H H 8.240 . . 233 105 114 VAL CA C 66.404 . . 234 105 114 VAL N N 121.269 . . 235 106 115 CYS H H 8.268 . . 236 106 115 CYS CA C 63.702 . . 237 106 115 CYS N N 117.136 . . 238 107 116 GLY H H 8.193 . . 239 107 116 GLY CA C 47.311 . . 240 107 116 GLY N N 104.672 . . 241 108 117 LEU H H 7.815 . . 242 108 117 LEU CA C 58.931 . . 243 108 117 LEU N N 119.217 . . 244 109 118 VAL H H 8.048 . . 245 109 118 VAL CA C 61.638 . . 246 109 118 VAL N N 122.597 . . 247 110 119 TRP H H 8.220 . . 248 110 119 TRP CA C 58.654 . . 249 110 119 TRP N N 119.010 . . 250 117 126 LYS H H 7.938 . . 251 117 126 LYS CA C 55.315 . . 252 117 126 LYS N N 120.023 . . 253 118 127 GLN H H 8.045 . . 254 118 127 GLN CA C 58.763 . . 255 118 127 GLN N N 119.533 . . 256 119 128 LYS H H 8.039 . . 257 119 128 LYS CA C 63.056 . . 258 119 128 LYS N N 121.261 . . 259 120 129 HIS H H 8.316 . . 260 120 129 HIS CA C 57.596 . . 261 120 129 HIS N N 120.580 . . 262 121 130 ARG H H 8.236 . . 263 121 130 ARG CA C 58.255 . . 264 121 130 ARG N N 120.326 . . 265 122 131 GLN H H 8.343 . . 266 122 131 GLN CA C 59.202 . . 267 122 131 GLN N N 120.105 . . 268 123 132 LYS H H 8.303 . . 269 123 132 LYS CA C 60.685 . . 270 123 132 LYS N N 118.832 . . 271 124 133 SER H H 8.350 . . 272 124 133 SER CA C 57.532 . . 273 124 133 SER N N 119.529 . . 274 125 134 ASN H H 8.125 . . 275 125 134 ASN CA C 61.850 . . 276 125 134 ASN CB C 40.791 . . 277 125 134 ASN N N 114.527 . . 278 126 135 PHE H H 7.357 . . 279 126 135 PHE CA C 63.992 . . 280 126 135 PHE CB C 36.902 . . 281 126 135 PHE N N 119.629 . . 282 127 136 LEU H H 8.120 . . 283 127 136 LEU CA C 58.524 . . 284 127 136 LEU CB C 40.561 . . 285 127 136 LEU N N 121.345 . . 286 128 137 ARG H H 8.200 . . 287 128 137 ARG CA C 58.960 . . 288 128 137 ARG CB C 31.889 . . 289 128 137 ARG N N 116.589 . . 290 129 138 SER H H 7.653 . . 291 129 138 SER CA C 61.701 . . 292 129 138 SER CB C 62.642 . . 293 129 138 SER N N 116.565 . . 294 130 139 LEU H H 8.395 . . 295 130 139 LEU CA C 57.577 . . 296 130 139 LEU CB C 40.643 . . 297 130 139 LEU N N 121.452 . . 298 131 140 LYS H H 8.060 . . 299 131 140 LYS CA C 59.888 . . 300 131 140 LYS CB C 32.568 . . 301 131 140 LYS N N 118.024 . . 302 132 141 SER H H 7.881 . . 303 132 141 SER CA C 60.778 . . 304 132 141 SER CB C 63.143 . . 305 132 141 SER N N 113.095 . . 306 133 142 PHE H H 7.745 . . 307 133 142 PHE CA C 60.090 . . 308 133 142 PHE CB C 39.340 . . 309 133 142 PHE N N 120.936 . . 310 134 143 PHE H H 7.680 . . 311 134 143 PHE CA C 59.537 . . 312 134 143 PHE CB C 40.527 . . 313 134 143 PHE N N 113.822 . . 314 135 144 LEU H H 7.790 . . 315 135 144 LEU CA C 54.856 . . 316 135 144 LEU CB C 42.592 . . 317 135 144 LEU N N 118.102 . . 318 136 145 THR H H 7.734 . . 319 136 145 THR CA C 61.881 . . 320 136 145 THR CB C 69.158 . . 321 136 145 THR N N 114.311 . . 322 137 146 ARG H H 7.677 . . 323 137 146 ARG CA C 57.056 . . 324 137 146 ARG CB C 30.510 . . stop_ save_