data_27968 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution NMR Backbone Resonance Assignments for p150Glued(1-191) of Dynactin ; _BMRB_accession_number 27968 _BMRB_flat_file_name bmr27968.str _Entry_type original _Submission_date 2019-07-05 _Accession_date 2019-07-05 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Guo Changmiao . . 2 Polenova Tatyana . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 176 "13C chemical shifts" 191 "15N chemical shifts" 176 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-02-05 update BMRB 'update entry citation' 2019-07-12 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 17937 'Solid-state chemical shift assignments for CAP-Gly domain' 17938 'Solution chemical shift assignments for CAP-Gly domain (p150Glued 19-107)' 19025 'Solid-state chemical shift assignments for CAP-Gly Domain at 19.9 T' 19031 'Solid-state NMR assignments of CAP-Gly with EB1' 25005 'MAS Structure of CAP-Gly Domain in complex with microtubules' 27973 'Solid-state NMR Chemical Shift Assignments for p150Glued(1-191) of Dynactin' stop_ _Original_release_date 2019-07-05 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Conformational Flexibility of p150Glued(1-191) Subunit of Dynactin Assembled with Microtubules ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 31445682 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Guo Changmiao . . 2 Williams John C. . 3 Polenova Tatyana . . stop_ _Journal_abbreviation 'Biophys. J.' _Journal_volume 117 _Journal_issue 5 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 938 _Page_last 949 _Year 2019 _Details . loop_ _Keyword dynactin microtubule p150Glued 'solution NMR' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'p150Glued(1-191)/microtubule complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label p150Glued(1-191) $Extended_CAP-Gly stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Extended_CAP-Gly _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Extended_CAP-Gly _Molecular_mass . _Mol_thiol_state 'all free' loop_ _Biological_function 'Assist dynein motility along microtubules' 'Microtubule-binding domains of dynactin' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 191 _Mol_residue_sequence ; MAQSKRHMYNRTPSGSRMST EASARPLRVGSRVEVIGKGH RGTVAYVGATLFATGKWVGV ILDEAKGKNDGTVQGRKYFT CDEGHGIFVRQSQIQVFEDG ADTTSPETPDSSASKILKRE GADAAAKTSKLRGLKPKKAP TARKTTTRRPKPTRPASTGV AGPSSSLGPSGSASAGELSS SEPSTPAQTPL ; loop_ _Residue_seq_code _Residue_label 1 MET 2 ALA 3 GLN 4 SER 5 LYS 6 ARG 7 HIS 8 MET 9 TYR 10 ASN 11 ARG 12 THR 13 PRO 14 SER 15 GLY 16 SER 17 ARG 18 MET 19 SER 20 THR 21 GLU 22 ALA 23 SER 24 ALA 25 ARG 26 PRO 27 LEU 28 ARG 29 VAL 30 GLY 31 SER 32 ARG 33 VAL 34 GLU 35 VAL 36 ILE 37 GLY 38 LYS 39 GLY 40 HIS 41 ARG 42 GLY 43 THR 44 VAL 45 ALA 46 TYR 47 VAL 48 GLY 49 ALA 50 THR 51 LEU 52 PHE 53 ALA 54 THR 55 GLY 56 LYS 57 TRP 58 VAL 59 GLY 60 VAL 61 ILE 62 LEU 63 ASP 64 GLU 65 ALA 66 LYS 67 GLY 68 LYS 69 ASN 70 ASP 71 GLY 72 THR 73 VAL 74 GLN 75 GLY 76 ARG 77 LYS 78 TYR 79 PHE 80 THR 81 CYS 82 ASP 83 GLU 84 GLY 85 HIS 86 GLY 87 ILE 88 PHE 89 VAL 90 ARG 91 GLN 92 SER 93 GLN 94 ILE 95 GLN 96 VAL 97 PHE 98 GLU 99 ASP 100 GLY 101 ALA 102 ASP 103 THR 104 THR 105 SER 106 PRO 107 GLU 108 THR 109 PRO 110 ASP 111 SER 112 SER 113 ALA 114 SER 115 LYS 116 ILE 117 LEU 118 LYS 119 ARG 120 GLU 121 GLY 122 ALA 123 ASP 124 ALA 125 ALA 126 ALA 127 LYS 128 THR 129 SER 130 LYS 131 LEU 132 ARG 133 GLY 134 LEU 135 LYS 136 PRO 137 LYS 138 LYS 139 ALA 140 PRO 141 THR 142 ALA 143 ARG 144 LYS 145 THR 146 THR 147 THR 148 ARG 149 ARG 150 PRO 151 LYS 152 PRO 153 THR 154 ARG 155 PRO 156 ALA 157 SER 158 THR 159 GLY 160 VAL 161 ALA 162 GLY 163 PRO 164 SER 165 SER 166 SER 167 LEU 168 GLY 169 PRO 170 SER 171 GLY 172 SER 173 ALA 174 SER 175 ALA 176 GLY 177 GLU 178 LEU 179 SER 180 SER 181 SER 182 GLU 183 PRO 184 SER 185 THR 186 PRO 187 ALA 188 GLN 189 THR 190 PRO 191 LEU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Extended_CAP-Gly Rat 10116 Eukaryota Metazoa Rattus norvegicus stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Extended_CAP-Gly 'recombinant technology' . Escherichia coli BL21(DE3) pET28b stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Extended_CAP-Gly 300 uM '[U-99% 13C; U-99% 15N]' phosphate 20 mM 'natural abundance' NaCl 50 mM 'natural abundance' DTT 1 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CcpNmr_Analysis _Saveframe_category software _Name CcpNmr_Analysis _Version . loop_ _Vendor _Address _Electronic_address 'Skinner, Goult, Fogh, Boucher, Stevens, Laue and Vuister' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.17 . M pH 6.0 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCA' '3D HN(CO)CA' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name p150Glued(1-191) _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 MET H H 8.292 . 1 2 1 1 MET CA C 55.704 . 1 3 1 1 MET N N 120.196 . 1 4 2 2 ALA H H 8.467 . 1 5 2 2 ALA CA C 52.851 . 1 6 2 2 ALA N N 125.789 . 1 7 3 3 GLN H H 8.374 . 1 8 3 3 GLN CA C 56.092 . 1 9 3 3 GLN N N 119.663 . 1 10 4 4 SER H H 8.331 . 1 11 4 4 SER CA C 58.612 . 1 12 4 4 SER N N 117.018 . 1 13 5 5 LYS H H 8.345 . 1 14 5 5 LYS CA C 56.344 . 1 15 5 5 LYS N N 123.042 . 1 16 6 6 ARG H H 8.419 . 1 17 6 6 ARG CA C 55.449 . 1 18 6 6 ARG N N 122.927 . 1 19 7 7 HIS H H 8.186 . 1 20 7 7 HIS CA C 56.398 . 1 21 7 7 HIS N N 121.181 . 1 22 8 8 MET H H 8.406 . 1 23 8 8 MET CA C 55.540 . 1 24 8 8 MET N N 121.367 . 1 25 9 9 TYR H H 8.172 . 1 26 9 9 TYR CA C 57.779 . 1 27 9 9 TYR N N 120.792 . 1 28 10 10 ASN H H 8.368 . 1 29 10 10 ASN CA C 53.142 . 1 30 10 10 ASN N N 120.511 . 1 31 11 11 ARG H H 8.143 . 1 32 11 11 ARG CA C 57.124 . 1 33 11 11 ARG N N 119.529 . 1 34 12 12 THR H H 7.996 . 1 35 12 12 THR CA C 62.461 . 1 36 12 12 THR N N 113.743 . 1 37 13 13 PRO CA C 63.616 . 1 38 14 14 SER H H 8.420 . 1 39 14 14 SER CA C 58.701 . 1 40 14 14 SER N N 115.928 . 1 41 15 15 GLY H H 8.429 . 1 42 15 15 GLY CA C 45.434 . 1 43 15 15 GLY N N 110.998 . 1 44 16 16 SER H H 8.233 . 1 45 16 16 SER CA C 58.389 . 1 46 16 16 SER N N 115.560 . 1 47 17 17 ARG H H 8.062 . 1 48 17 17 ARG CA C 53.823 . 1 49 17 17 ARG N N 121.733 . 1 50 18 18 MET H H 8.473 . 1 51 18 18 MET CA C 55.543 . 1 52 18 18 MET N N 119.992 . 1 53 19 19 SER H H 8.394 . 1 54 19 19 SER CA C 58.443 . 1 55 19 19 SER N N 117.187 . 1 56 20 20 THR H H 8.236 . 1 57 20 20 THR CA C 62.191 . 1 58 20 20 THR N N 115.584 . 1 59 21 21 GLU H H 8.388 . 1 60 21 21 GLU CA C 56.983 . 1 61 21 21 GLU N N 122.928 . 1 62 22 22 ALA H H 8.322 . 1 63 22 22 ALA CA C 53.143 . 1 64 22 22 ALA N N 124.740 . 1 65 23 23 SER H H 8.140 . 1 66 23 23 SER CA C 58.574 . 1 67 23 23 SER N N 114.121 . 1 68 24 24 ALA H H 8.104 . 1 69 24 24 ALA CA C 52.339 . 1 70 24 24 ALA N N 125.473 . 1 71 25 25 ARG H H 8.140 . 1 72 25 25 ARG CA C 54.000 . 1 73 25 25 ARG N N 121.420 . 1 74 26 26 PRO CA C 62.907 . 1 75 27 27 LEU H H 8.189 . 1 76 27 27 LEU CA C 54.945 . 1 77 27 27 LEU N N 122.709 . 1 78 28 28 ARG H H 8.392 . 1 79 28 28 ARG CA C 53.947 . 1 80 28 28 ARG N N 122.789 . 1 81 29 29 VAL H H 8.582 . 1 82 29 29 VAL CA C 66.101 . 1 83 29 29 VAL N N 122.491 . 1 84 30 30 GLY H H 8.922 . 1 85 30 30 GLY CA C 44.649 . 1 86 30 30 GLY N N 116.697 . 1 87 31 31 SER H H 8.251 . 1 88 31 31 SER CA C 61.232 . 1 89 31 31 SER N N 117.835 . 1 90 32 32 ARG H H 8.925 . 1 91 32 32 ARG CA C 53.412 . 1 92 32 32 ARG N N 125.175 . 1 93 33 33 VAL H H 8.730 . 1 94 33 33 VAL CA C 57.993 . 1 95 33 33 VAL N N 112.404 . 1 96 34 34 GLU H H 8.794 . 1 97 34 34 GLU CA C 53.843 . 1 98 34 34 GLU N N 118.888 . 1 99 35 35 VAL H H 8.658 . 1 100 35 35 VAL CA C 62.956 . 1 101 35 35 VAL N N 124.382 . 1 102 36 36 ILE H H 8.297 . 1 103 36 36 ILE CA C 64.030 . 1 104 36 36 ILE N N 131.996 . 1 105 37 37 GLY H H 9.102 . 1 106 37 37 GLY CA C 45.615 . 1 107 37 37 GLY N N 117.385 . 1 108 38 38 LYS H H 7.552 . 1 109 38 38 LYS CA C 55.664 . 1 110 38 38 LYS N N 117.353 . 1 111 39 39 GLY H H 8.178 . 1 112 39 39 GLY CA C 45.566 . 1 113 39 39 GLY N N 105.629 . 1 114 40 40 HIS H H 6.199 . 1 115 40 40 HIS CA C 54.926 . 1 116 40 40 HIS N N 116.100 . 1 117 41 41 ARG H H 8.897 . 1 118 41 41 ARG CA C 55.101 . 1 119 41 41 ARG N N 122.640 . 1 120 42 42 GLY H H 8.914 . 1 121 42 42 GLY CA C 47.054 . 1 122 42 42 GLY N N 107.357 . 1 123 43 43 THR H H 8.463 . 1 124 43 43 THR CA C 60.620 . 1 125 43 43 THR N N 116.765 . 1 126 44 44 VAL H H 9.316 . 1 127 44 44 VAL CA C 65.164 . 1 128 44 44 VAL N N 127.522 . 1 129 45 45 ALA H H 9.119 . 1 130 45 45 ALA CA C 51.584 . 1 131 45 45 ALA N N 131.826 . 1 132 46 46 TYR H H 7.622 . 1 133 46 46 TYR CA C 58.575 . 1 134 46 46 TYR N N 119.545 . 1 135 47 47 VAL H H 8.146 . 1 136 47 47 VAL CA C 61.596 . 1 137 47 47 VAL N N 127.962 . 1 138 48 48 GLY H H 8.935 . 1 139 48 48 GLY CA C 45.123 . 1 140 48 48 GLY N N 112.718 . 1 141 49 49 ALA H H 8.724 . 1 142 49 49 ALA CA C 51.691 . 1 143 49 49 ALA N N 123.589 . 1 144 50 50 THR H H 10.126 . 1 145 50 50 THR CA C 59.469 . 1 146 50 50 THR N N 112.059 . 1 147 51 51 LEU H H 7.731 . 1 148 51 51 LEU CA C 54.745 . 1 149 51 51 LEU N N 113.360 . 1 150 52 52 PHE H H 6.439 . 1 151 52 52 PHE CA C 56.230 . 1 152 52 52 PHE N N 111.387 . 1 153 53 53 ALA H H 6.778 . 1 154 53 53 ALA CA C 51.960 . 1 155 53 53 ALA N N 123.142 . 1 156 54 54 THR H H 8.415 . 1 157 54 54 THR CA C 61.946 . 1 158 54 54 THR N N 110.402 . 1 159 55 55 GLY H H 8.805 . 1 160 55 55 GLY CA C 44.974 . 1 161 55 55 GLY N N 109.034 . 1 162 56 56 LYS H H 8.509 . 1 163 56 56 LYS CA C 56.669 . 1 164 56 56 LYS N N 120.808 . 1 165 57 57 TRP H H 9.149 . 1 166 57 57 TRP CA C 56.655 . 1 167 57 57 TRP N N 128.649 . 1 168 58 58 VAL H H 9.487 . 1 169 58 58 VAL CA C 60.868 . 1 170 58 58 VAL N N 119.599 . 1 171 59 59 GLY H H 10.013 . 1 172 59 59 GLY CA C 45.687 . 1 173 59 59 GLY N N 115.451 . 1 174 60 60 VAL H H 9.071 . 1 175 60 60 VAL CA C 60.653 . 1 176 60 60 VAL N N 128.290 . 1 177 61 61 ILE H H 8.612 . 1 178 61 61 ILE CA C 60.508 . 1 179 61 61 ILE N N 124.549 . 1 180 62 62 LEU H H 10.138 . 1 181 62 62 LEU CA C 55.440 . 1 182 62 62 LEU N N 132.377 . 1 183 63 63 ASP H H 8.994 . 1 184 63 63 ASP CA C 57.230 . 1 185 63 63 ASP N N 124.856 . 1 186 64 64 GLU H H 8.671 . 1 187 64 64 GLU CA C 54.387 . 1 188 64 64 GLU N N 117.380 . 1 189 65 65 ALA H H 8.474 . 1 190 65 65 ALA CA C 50.826 . 1 191 65 65 ALA N N 124.786 . 1 192 66 66 LYS H H 7.818 . 1 193 66 66 LYS CA C 53.806 . 1 194 66 66 LYS N N 122.258 . 1 195 67 67 GLY H H 8.552 . 1 196 67 67 GLY CA C 44.121 . 1 197 67 67 GLY N N 108.893 . 1 198 68 68 LYS H H 8.297 . 1 199 68 68 LYS CA C 55.878 . 1 200 68 68 LYS N N 116.086 . 1 201 69 69 ASN H H 8.463 . 1 202 69 69 ASN CA C 53.280 . 1 203 69 69 ASN N N 116.677 . 1 204 70 70 ASP H H 8.145 . 1 205 70 70 ASP CA C 52.870 . 1 206 70 70 ASP N N 119.196 . 1 207 71 71 GLY H H 8.741 . 1 208 71 71 GLY CA C 43.450 . 1 209 71 71 GLY N N 109.805 . 1 210 72 72 THR H H 7.807 . 1 211 72 72 THR CA C 61.305 . 1 212 72 72 THR N N 117.056 . 1 213 73 73 VAL H H 8.449 . 1 214 73 73 VAL CA C 61.720 . 1 215 73 73 VAL N N 122.615 . 1 216 74 74 GLN H H 9.823 . 1 217 74 74 GLN CA C 56.705 . 1 218 74 74 GLN N N 129.493 . 1 219 75 75 GLY H H 8.576 . 1 220 75 75 GLY CA C 45.446 . 1 221 75 75 GLY N N 103.612 . 1 222 76 76 ARG H H 7.565 . 1 223 76 76 ARG CA C 53.680 . 1 224 76 76 ARG N N 121.818 . 1 225 77 77 LYS H H 8.456 . 1 226 77 77 LYS CA C 56.256 . 1 227 77 77 LYS N N 127.179 . 1 228 78 78 TYR H H 8.785 . 1 229 78 78 TYR CA C 60.453 . 1 230 78 78 TYR N N 127.394 . 1 231 79 79 PHE H H 7.957 . 1 232 79 79 PHE CA C 55.748 . 1 233 79 79 PHE N N 113.687 . 1 234 80 80 THR H H 8.647 . 1 235 80 80 THR CA C 62.374 . 1 236 80 80 THR N N 114.475 . 1 237 81 81 CYS H H 7.930 . 1 238 81 81 CYS CA C 56.044 . 1 239 81 81 CYS N N 120.559 . 1 240 82 82 ASP H H 8.236 . 1 241 82 82 ASP CA C 54.486 . 1 242 82 82 ASP N N 118.031 . 1 243 83 83 GLU H H 8.768 . 1 244 83 83 GLU CA C 58.285 . 1 245 83 83 GLU N N 121.244 . 1 246 84 84 GLY H H 8.150 . 1 247 84 84 GLY CA C 45.262 . 1 248 84 84 GLY N N 111.716 . 1 249 85 85 HIS H H 8.165 . 1 250 85 85 HIS CA C 56.364 . 1 251 85 85 HIS N N 113.696 . 1 252 86 86 GLY H H 9.082 . 1 253 86 86 GLY CA C 45.409 . 1 254 86 86 GLY N N 111.993 . 1 255 87 87 ILE H H 8.912 . 1 256 87 87 ILE CA C 60.484 . 1 257 87 87 ILE N N 119.199 . 1 258 88 88 PHE H H 8.877 . 1 259 88 88 PHE CA C 57.725 . 1 260 88 88 PHE N N 122.768 . 1 261 89 89 VAL H H 9.582 . 1 262 89 89 VAL CA C 58.938 . 1 263 89 89 VAL N N 115.258 . 1 264 90 90 ARG H H 8.668 . 1 265 90 90 ARG CA C 53.554 . 1 266 90 90 ARG N N 117.753 . 1 267 91 91 GLN H H 9.393 . 1 268 91 91 GLN CA C 59.650 . 1 269 91 91 GLN N N 121.845 . 1 270 92 92 SER H H 7.624 . 1 271 92 92 SER CA C 59.398 . 1 272 92 92 SER N N 108.823 . 1 273 93 93 GLN H H 7.870 . 1 274 93 93 GLN CA C 56.384 . 1 275 93 93 GLN N N 118.129 . 1 276 94 94 ILE H H 7.510 . 1 277 94 94 ILE CA C 58.241 . 1 278 94 94 ILE N N 112.903 . 1 279 95 95 GLN H H 8.602 . 1 280 95 95 GLN CA C 53.615 . 1 281 95 95 GLN N N 118.653 . 1 282 96 96 VAL H H 8.763 . 1 283 96 96 VAL CA C 62.593 . 1 284 96 96 VAL N N 125.362 . 1 285 97 97 PHE H H 8.408 . 1 286 97 97 PHE CA C 56.843 . 1 287 97 97 PHE N N 125.587 . 1 288 98 98 GLU H H 8.395 . 1 289 98 98 GLU CA C 55.901 . 1 290 98 98 GLU N N 122.006 . 1 291 99 99 ASP H H 8.510 . 1 292 99 99 ASP CA C 54.708 . 1 293 99 99 ASP N N 123.262 . 1 294 100 100 GLY H H 8.522 . 1 295 100 100 GLY CA C 45.450 . 1 296 100 100 GLY N N 109.914 . 1 297 101 101 ALA H H 8.155 . 1 298 101 101 ALA CA C 52.644 . 1 299 101 101 ALA N N 123.276 . 1 300 102 102 ASP H H 8.440 . 1 301 102 102 ASP CA C 54.234 . 1 302 102 102 ASP N N 119.181 . 1 303 103 103 THR H H 8.086 . 1 304 103 103 THR CA C 61.749 . 1 305 103 103 THR N N 114.221 . 1 306 104 104 THR H H 8.189 . 1 307 104 104 THR CA C 62.144 . 1 308 104 104 THR N N 116.125 . 1 309 105 105 SER H H 8.379 . 1 310 105 105 SER CA C 56.457 . 1 311 105 105 SER N N 119.716 . 1 312 106 106 PRO CA C 62.069 . 1 313 107 107 GLU H H 8.063 . 1 314 107 107 GLU CA C 55.693 . 1 315 107 107 GLU N N 118.048 . 1 316 108 108 THR H H 7.943 . 1 317 108 108 THR CA C 58.987 . 1 318 108 108 THR N N 115.311 . 1 319 109 109 PRO CA C 63.573 . 1 320 110 110 ASP H H 8.410 . 1 321 110 110 ASP CA C 54.477 . 1 322 110 110 ASP N N 120.005 . 1 323 111 111 SER H H 8.342 . 1 324 111 111 SER CA C 58.985 . 1 325 111 111 SER N N 117.304 . 1 326 112 112 SER H H 8.430 . 1 327 112 112 SER CA C 59.547 . 1 328 112 112 SER N N 118.052 . 1 329 113 113 ALA H H 8.163 . 1 330 113 113 ALA CA C 53.301 . 1 331 113 113 ALA N N 125.128 . 1 332 114 114 SER H H 8.095 . 1 333 114 114 SER CA C 59.077 . 1 334 114 114 SER N N 113.930 . 1 335 115 115 LYS H H 8.111 . 1 336 115 115 LYS CA C 56.852 . 1 337 115 115 LYS N N 122.753 . 1 338 116 116 ILE H H 7.926 . 1 339 116 116 ILE CA C 61.562 . 1 340 116 116 ILE N N 120.993 . 1 341 117 117 LEU H H 8.168 . 1 342 117 117 LEU CA C 55.330 . 1 343 117 117 LEU N N 125.851 . 1 344 118 118 LYS H H 7.894 . 1 345 118 118 LYS CA C 56.891 . 1 346 118 118 LYS N N 123.490 . 1 347 119 119 ARG H H 8.054 . 1 348 119 119 ARG CA C 55.380 . 1 349 119 119 ARG N N 121.754 . 1 350 120 120 GLU H H 8.219 . 1 351 120 120 GLU CA C 56.450 . 1 352 120 120 GLU N N 122.483 . 1 353 121 121 GLY H H 8.303 . 1 354 121 121 GLY CA C 45.367 . 1 355 121 121 GLY N N 109.307 . 1 356 122 122 ALA H H 8.221 . 1 357 122 122 ALA CA C 53.188 . 1 358 122 122 ALA N N 123.803 . 1 359 123 123 ASP H H 8.363 . 1 360 123 123 ASP CA C 54.653 . 1 361 123 123 ASP N N 118.968 . 1 362 124 124 ALA H H 8.123 . 1 363 124 124 ALA CA C 53.518 . 1 364 124 124 ALA N N 124.278 . 1 365 125 125 ALA H H 8.142 . 1 366 125 125 ALA CA C 53.125 . 1 367 125 125 ALA N N 121.726 . 1 368 126 126 ALA H H 7.996 . 1 369 126 126 ALA CA C 53.143 . 1 370 126 126 ALA N N 122.050 . 1 371 127 127 LYS H H 8.237 . 1 372 127 127 LYS CA C 56.014 . 1 373 127 127 LYS N N 121.423 . 1 374 128 128 THR H H 8.269 . 1 375 128 128 THR CA C 59.975 . 1 376 128 128 THR N N 117.653 . 1 377 129 129 SER CA C 58.418 . 1 378 130 130 LYS H H 8.381 . 1 379 130 130 LYS CA C 58.448 . 1 380 130 130 LYS N N 118.008 . 1 381 131 131 LEU H H 8.415 . 1 382 131 131 LEU CA C 56.292 . 1 383 131 131 LEU N N 122.833 . 1 384 132 132 ARG H H 8.550 . 1 385 132 132 ARG CA C 56.892 . 1 386 132 132 ARG N N 122.297 . 1 387 133 133 GLY H H 8.486 . 1 388 133 133 GLY CA C 45.349 . 1 389 133 133 GLY N N 110.101 . 1 390 134 134 LEU H H 7.995 . 1 391 134 134 LEU CA C 54.998 . 1 392 134 134 LEU N N 121.471 . 1 393 135 135 LYS H H 8.338 . 1 394 135 135 LYS CA C 54.120 . 1 395 135 135 LYS N N 123.760 . 1 396 136 136 PRO CA C 62.961 . 1 397 137 137 LYS H H 8.436 . 1 398 137 137 LYS CA C 56.334 . 1 399 137 137 LYS N N 122.312 . 1 400 138 138 LYS H H 8.320 . 1 401 138 138 LYS CA C 55.933 . 1 402 138 138 LYS N N 123.188 . 1 403 139 139 ALA H H 8.431 . 1 404 139 139 ALA CA C 50.529 . 1 405 139 139 ALA N N 127.750 . 1 406 140 140 PRO CA C 63.028 . 1 407 141 141 THR H H 8.231 . 1 408 141 141 THR CA C 61.945 . 1 409 141 141 THR N N 115.037 . 1 410 142 142 ALA H H 8.344 . 1 411 142 142 ALA CA C 52.371 . 1 412 142 142 ALA N N 127.189 . 1 413 143 143 ARG H H 8.355 . 1 414 143 143 ARG CA C 56.054 . 1 415 143 143 ARG N N 121.297 . 1 416 144 144 LYS H H 8.478 . 1 417 144 144 LYS CA C 56.299 . 1 418 144 144 LYS N N 123.616 . 1 419 145 145 THR H H 8.305 . 1 420 145 145 THR CA C 61.756 . 1 421 145 145 THR N N 116.362 . 1 422 146 146 THR H H 8.296 . 1 423 146 146 THR CA C 61.710 . 1 424 146 146 THR N N 116.911 . 1 425 147 147 THR H H 8.243 . 1 426 147 147 THR CA C 61.995 . 1 427 147 147 THR N N 117.720 . 1 428 148 148 ARG H H 8.443 . 1 429 148 148 ARG CA C 55.981 . 1 430 148 148 ARG N N 124.546 . 1 431 149 149 ARG H H 8.469 . 1 432 149 149 ARG CA C 53.927 . 1 433 149 149 ARG N N 124.313 . 1 434 150 150 PRO CA C 63.326 . 1 435 151 151 LYS H H 8.458 . 1 436 151 151 LYS CA C 56.588 . 1 437 151 151 LYS N N 120.811 . 1 438 152 152 PRO CA C 63.135 . 1 439 153 153 THR H H 8.320 . 1 440 153 153 THR CA C 61.966 . 1 441 153 153 THR N N 115.435 . 1 442 154 154 ARG H H 8.320 . 1 443 154 154 ARG CA C 53.866 . 1 444 154 154 ARG N N 124.254 . 1 445 155 155 PRO CA C 63.046 . 1 446 156 156 ALA H H 8.480 . 1 447 156 156 ALA CA C 52.576 . 1 448 156 156 ALA N N 124.554 . 1 449 157 157 SER H H 8.356 . 1 450 157 157 SER CA C 58.201 . 1 451 157 157 SER N N 115.025 . 1 452 158 158 THR H H 8.185 . 1 453 158 158 THR CA C 61.958 . 1 454 158 158 THR N N 115.197 . 1 455 159 159 GLY H H 8.367 . 1 456 159 159 GLY CA C 45.320 . 1 457 159 159 GLY N N 111.000 . 1 458 160 160 VAL H H 7.976 . 1 459 160 160 VAL CA C 62.114 . 1 460 160 160 VAL N N 119.144 . 1 461 161 161 ALA H H 8.419 . 1 462 161 161 ALA CA C 52.394 . 1 463 161 161 ALA N N 127.929 . 1 464 162 162 GLY H H 8.178 . 1 465 162 162 GLY CA C 44.558 . 1 466 162 162 GLY N N 108.516 . 1 467 163 163 PRO CA C 63.452 . 1 468 164 164 SER H H 8.499 . 1 469 164 164 SER CA C 58.606 . 1 470 164 164 SER N N 115.933 . 1 471 165 165 SER H H 8.469 . 1 472 165 165 SER CA C 58.436 . 1 473 165 165 SER N N 117.769 . 1 474 166 166 SER H H 8.331 . 1 475 166 166 SER CA C 58.468 . 1 476 166 166 SER N N 117.639 . 1 477 167 167 LEU H H 8.199 . 1 478 167 167 LEU CA C 55.202 . 1 479 167 167 LEU N N 123.571 . 1 480 168 168 GLY H H 8.152 . 1 481 168 168 GLY CA C 44.619 . 1 482 168 168 GLY N N 109.182 . 1 483 169 169 PRO CA C 62.632 . 1 484 170 170 SER H H 8.676 . 1 485 170 170 SER CA C 58.620 . 1 486 170 170 SER N N 117.154 . 1 487 171 171 GLY H H 8.379 . 1 488 171 171 GLY CA C 45.494 . 1 489 171 171 GLY N N 110.741 . 1 490 172 172 SER H H 8.179 . 1 491 172 172 SER CA C 58.357 . 1 492 172 172 SER N N 115.589 . 1 493 173 173 ALA H H 8.398 . 1 494 173 173 ALA CA C 52.782 . 1 495 173 173 ALA N N 126.006 . 1 496 174 174 SER H H 8.250 . 1 497 174 174 SER CA C 58.335 . 1 498 174 174 SER N N 114.985 . 1 499 175 175 ALA H H 8.318 . 1 500 175 175 ALA CA C 52.944 . 1 501 175 175 ALA N N 125.838 . 1 502 176 176 GLY H H 8.299 . 1 503 176 176 GLY CA C 45.334 . 1 504 176 176 GLY N N 107.756 . 1 505 177 177 GLU H H 8.214 . 1 506 177 177 GLU CA C 56.604 . 1 507 177 177 GLU N N 120.481 . 1 508 178 178 LEU H H 8.319 . 1 509 178 178 LEU CA C 55.259 . 1 510 178 178 LEU N N 122.980 . 1 511 179 179 SER H H 8.310 . 1 512 179 179 SER CA C 58.387 . 1 513 179 179 SER N N 116.521 . 1 514 180 180 SER H H 8.023 . 1 515 180 180 SER CA C 58.741 . 1 516 180 180 SER N N 116.048 . 1 517 181 181 SER H H 8.059 . 1 518 181 181 SER CA C 58.989 . 1 519 181 181 SER N N 116.096 . 1 520 182 182 GLU H H 8.272 . 1 521 182 182 GLU CA C 56.889 . 1 522 182 182 GLU N N 123.024 . 1 523 183 183 PRO CA C 63.287 . 1 524 184 184 SER H H 8.451 . 1 525 184 184 SER CA C 58.320 . 1 526 184 184 SER N N 116.291 . 1 527 185 185 THR H H 8.160 . 1 528 185 185 THR CA C 59.843 . 1 529 185 185 THR N N 118.068 . 1 530 186 186 PRO CA C 63.251 . 1 531 187 187 ALA H H 8.398 . 1 532 187 187 ALA CA C 52.542 . 1 533 187 187 ALA N N 124.319 . 1 534 188 188 GLN H H 8.348 . 1 535 188 188 GLN CA C 55.552 . 1 536 188 188 GLN N N 119.529 . 1 537 189 189 THR H H 8.224 . 1 538 189 189 THR CA C 60.005 . 1 539 189 189 THR N N 118.631 . 1 540 190 190 PRO CA C 62.924 . 1 541 191 191 LEU H H 8.420 . 1 542 191 191 LEU CA C 54.350 . 1 543 191 191 LEU N N 123.001 . 1 stop_ save_