data_27973 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solid-state NMR Chemical Shift Assignments for p150Glued(1-191) of Dynactin ; _BMRB_accession_number 27973 _BMRB_flat_file_name bmr27973.str _Entry_type original _Submission_date 2019-07-12 _Accession_date 2019-07-12 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'Resonance assignments for residues 1-191 in p150Glued subunit of dynactin' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Guo Changmiao . . 2 Polenova Tatyana . . 3 Williams John C. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "13C chemical shifts" 296 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-02-05 update BMRB 'update entry citation' 2019-08-15 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 17937 'Solid-state chemical shift assignments for CAP-Gly domain' 17938 'Solution chemical shift assignments for CAP-Gly domain (p150Glued 19-107)' 19025 'Solid-state chemical shift assignments for CAP-Gly Domain at 19.9 T' 25005 'MAS Structure of CAP-Gly Domain in complex with microtubules' 27968 'Solution NMR Backbone Resonance Assignments for p150Glued(1-191) of Dynactin' stop_ _Original_release_date 2019-07-15 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Conformational Flexibility of p150Glued(1-191) Subunit of Dynactin Assembled with Microtubules ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 31445682 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Guo Changmiao . . 2 Williams John C. . 3 Polenova Tatyana . . stop_ _Journal_abbreviation 'Biophys. J.' _Journal_volume 117 _Journal_issue 5 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 938 _Page_last 949 _Year 2019 _Details . loop_ _Keyword 'magic-angle spinning NMR' microtubule p150Glued 'solution NMR' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'p150Glued(1-191) in complex with microtubules' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'p150Glued(1-191) Subunit of Dynactin' $p150Glued(1-191) stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_p150Glued(1-191) _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common p150Glued(1-191) _Molecular_mass . _Mol_thiol_state 'all free' loop_ _Biological_function 'Assist dynein motility along microtubules' 'Microtubule-binding domains of dynactin' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 191 _Mol_residue_sequence ; MAQSKRHMYNRTPSGSRMST EASARPLRVGSRVEVIGKGH RGTVAYVGATLFATGKWVGV ILDEAKGKNDGTVQGRKYFT CDEGHGIFVRQSQIQVFEDG ADTTSPETPDSSASKILKRE GADAAAKTSKLRGLKPKKAP TARKTTTRRPKPTRPASTGV AGPSSSLGPSGSASAGELSS SEPSTPAQTPL ; loop_ _Residue_seq_code _Residue_label 1 MET 2 ALA 3 GLN 4 SER 5 LYS 6 ARG 7 HIS 8 MET 9 TYR 10 ASN 11 ARG 12 THR 13 PRO 14 SER 15 GLY 16 SER 17 ARG 18 MET 19 SER 20 THR 21 GLU 22 ALA 23 SER 24 ALA 25 ARG 26 PRO 27 LEU 28 ARG 29 VAL 30 GLY 31 SER 32 ARG 33 VAL 34 GLU 35 VAL 36 ILE 37 GLY 38 LYS 39 GLY 40 HIS 41 ARG 42 GLY 43 THR 44 VAL 45 ALA 46 TYR 47 VAL 48 GLY 49 ALA 50 THR 51 LEU 52 PHE 53 ALA 54 THR 55 GLY 56 LYS 57 TRP 58 VAL 59 GLY 60 VAL 61 ILE 62 LEU 63 ASP 64 GLU 65 ALA 66 LYS 67 GLY 68 LYS 69 ASN 70 ASP 71 GLY 72 THR 73 VAL 74 GLN 75 GLY 76 ARG 77 LYS 78 TYR 79 PHE 80 THR 81 CYS 82 ASP 83 GLU 84 GLY 85 HIS 86 GLY 87 ILE 88 PHE 89 VAL 90 ARG 91 GLN 92 SER 93 GLN 94 ILE 95 GLN 96 VAL 97 PHE 98 GLU 99 ASP 100 GLY 101 ALA 102 ASP 103 THR 104 THR 105 SER 106 PRO 107 GLU 108 THR 109 PRO 110 ASP 111 SER 112 SER 113 ALA 114 SER 115 LYS 116 ILE 117 LEU 118 LYS 119 ARG 120 GLU 121 GLY 122 ALA 123 ASP 124 ALA 125 ALA 126 ALA 127 LYS 128 THR 129 SER 130 LYS 131 LEU 132 ARG 133 GLY 134 LEU 135 LYS 136 PRO 137 LYS 138 LYS 139 ALA 140 PRO 141 THR 142 ALA 143 ARG 144 LYS 145 THR 146 THR 147 THR 148 ARG 149 ARG 150 PRO 151 LYS 152 PRO 153 THR 154 ARG 155 PRO 156 ALA 157 SER 158 THR 159 GLY 160 VAL 161 ALA 162 GLY 163 PRO 164 SER 165 SER 166 SER 167 LEU 168 GLY 169 PRO 170 SER 171 GLY 172 SER 173 ALA 174 SER 175 ALA 176 GLY 177 GLU 178 LEU 179 SER 180 SER 181 SER 182 GLU 183 PRO 184 SER 185 THR 186 PRO 187 ALA 188 GLN 189 THR 190 PRO 191 LEU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $p150Glued(1-191) Rat 10116 Eukaryota Metazoa Rattus norvegicus stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $p150Glued(1-191) 'recombinant technology' . Escherichia coli BL21(DE3) pET28b stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_complexes _Saveframe_category sample _Sample_type solid-state _Details 'The microtubules is obtained from a vendor; purified from Bovine brain.' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $p150Glued(1-191) 450 uM '[U-100% 13C; U-100% 15N]' microtubules 540 uM 'natural abundance' PIPES 80 mM 'natural abundance' MgCl2 1 mM 'natural abundance' EGTA 1 mM 'natural abundance' stop_ save_ save_sample_microtubules _Saveframe_category sample _Sample_type solid-state _Details 'Polymerized microtubules' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling microtubules 540 uM 'natural abundance' PIPES 80 mM 'natural abundance' MgCl2 1 mM 'natural abundance' EGTA 1 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_VNMR _Saveframe_category software _Name VNMR _Version . loop_ _Vendor _Address _Electronic_address Varian . . stop_ loop_ _Task collection stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model 'Uniform NMR System' _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_13C-13C_CORD_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 13C-13C CORD' _Sample_label $sample_complexes save_ save_2D_NCA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NCA' _Sample_label $sample_complexes save_ save_2D_NCO_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NCO' _Sample_label $sample_complexes save_ save_1H-31P_CPMAS_4 _Saveframe_category NMR_applied_experiment _Experiment_name '1H-31P CPMAS' _Sample_label $sample_microtubules save_ save_31P_direct_polarization_MAS_NMR_5 _Saveframe_category NMR_applied_experiment _Experiment_name '31P direct polarization MAS NMR' _Sample_label $sample_microtubules save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details 'BRB 80 Buffer , 80 mM PIPES, 1 mM MgCl2, 1mM EGTA, pH 6.8' loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.17 . M pH 6.8 . pH pressure 1 . atm temperature 273 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.8 . pH pressure 1 . atm temperature 273 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 DSS P 31 'methyl protons' ppm 0.00 na indirect . . . 0.404808636 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_13_and_15N_chemical_shifts_of_p150Glued(1-191)_assembled_on_microtubules _Saveframe_category assigned_chemical_shifts _Details ; The 31P chemical shifts of microtubules: 1 GTP - OPA -8.1 2 GTP - OPB -18.0 3 GTP - OPG -6.2 4 GDP - OPA -10.3 5 GDP - OPB -5.1 6 Pi - OP 1.7 ; loop_ _Experiment_label '2D 13C-13C CORD' '2D NCA' '2D NCO' stop_ loop_ _Sample_label $sample_complexes stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'p150Glued(1-191) Subunit of Dynactin' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 11 11 ARG CA C 57.45 . 1 2 11 11 ARG CB C 30.98 . 1 3 11 11 ARG CG C 28.12 . 1 4 12 12 THR CA C 65.29 . 1 5 12 12 THR CB C 70.38 . 1 6 14 14 SER CA C 58.79 . 9 7 14 14 SER CB C 65.22 . 9 8 17 17 ARG CA C 53.73 . 1 9 17 17 ARG CB C 34.15 . 1 10 20 20 THR CA C 64.88 . 1 11 20 20 THR CB C 69.06 . 1 12 20 20 THR CG2 C 22.81 . 1 13 21 21 GLU CA C 57.47 . 1 14 21 21 GLU CB C 29.51 . 1 15 21 21 GLU CG C 34.21 . 1 16 24 24 ALA CA C 53.1 . 1 17 24 24 ALA CB C 19.86 . 1 18 26 26 PRO CB C 32.78 . 1 19 26 26 PRO CD C 50.65 . 1 20 27 27 LEU CA C 55.03 . 1 21 27 27 LEU CB C 41.43 . 1 22 28 28 ARG CA C 53.86 . 1 23 28 28 ARG CB C 30.99 . 1 24 29 29 VAL CB C 31.98 . 1 25 29 29 VAL CG1 C 22.74 . 1 26 29 29 VAL CG2 C 21.35 . 1 27 31 31 SER CA C 61.76 . 1 28 31 31 SER CB C 63.83 . 1 29 33 33 VAL CA C 58.01 . 1 30 33 33 VAL CB C 38.19 . 1 31 33 33 VAL CG1 C 23.84 . 1 32 34 34 GLU CA C 53.5 . 1 33 34 34 GLU CB C 32.43 . 1 34 35 35 VAL CA C 63.57 . 1 35 35 35 VAL CB C 32.95 . 1 36 35 35 VAL CG1 C 22.42 . 1 37 36 36 ILE CA C 64.02 . 1 38 36 36 ILE CB C 38.97 . 1 39 36 36 ILE CG1 C 29.28 . 1 40 36 36 ILE CG2 C 17.37 . 1 41 36 36 ILE CD1 C 14.5 . 1 42 38 38 LYS CA C 55.46 . 1 43 38 38 LYS CB C 31.1 . 1 44 40 40 HIS CA C 55.16 . 1 45 40 40 HIS CB C 33.19 . 1 46 41 41 ARG CA C 55.17 . 1 47 41 41 ARG CB C 31.89 . 1 48 43 43 THR CA C 60.45 . 1 49 43 43 THR CB C 71.24 . 1 50 43 43 THR CG2 C 22.48 . 1 51 44 44 VAL CA C 65.07 . 1 52 44 44 VAL CB C 31.19 . 1 53 44 44 VAL CG2 C 22.67 . 1 54 45 45 ALA CA C 51.73 . 1 55 45 45 ALA CB C 22.03 . 1 56 46 46 TYR CA C 58.77 . 1 57 46 46 TYR CB C 42.78 . 1 58 47 47 VAL CA C 61.94 . 1 59 47 47 VAL CB C 35.28 . 1 60 47 47 VAL CG1 C 22.1 . 1 61 49 49 ALA CA C 51.89 . 1 62 49 49 ALA CB C 19.96 . 1 63 50 50 THR CA C 59.36 . 1 64 50 50 THR CB C 72.34 . 1 65 50 50 THR CG2 C 22.41 . 1 66 51 51 LEU CA C 55.01 . 1 67 51 51 LEU CG C 27.25 . 1 68 52 52 PHE CA C 55.92 . 1 69 52 52 PHE CB C 38.62 . 1 70 54 54 THR CA C 62.55 . 1 71 54 54 THR CB C 70.59 . 1 72 54 54 THR CG2 C 22.28 . 1 73 56 56 LYS CA C 57.48 . 1 74 56 56 LYS CB C 33.78 . 1 75 56 56 LYS CG C 25.38 . 1 76 56 56 LYS CE C 41.13 . 1 77 57 57 TRP CA C 57.22 . 1 78 57 57 TRP CB C 33.73 . 1 79 58 58 VAL CA C 61.13 . 1 80 58 58 VAL CB C 33.19 . 1 81 60 60 VAL CA C 60.35 . 1 82 60 60 VAL CB C 34.77 . 1 83 60 60 VAL CG1 C 22.17 . 1 84 60 60 VAL CG2 C 21.88 . 1 85 61 61 ILE CA C 60.31 . 1 86 61 61 ILE CG1 C 28.64 . 1 87 61 61 ILE CG2 C 18.24 . 1 88 61 61 ILE CD1 C 15.34 . 1 89 62 62 LEU CA C 55.56 . 1 90 62 62 LEU CB C 41.6 . 1 91 62 62 LEU CG C 27.08 . 1 92 62 62 LEU CD1 C 22.47 . 1 93 62 62 LEU CD2 C 22.47 . 1 94 64 64 GLU CA C 54.12 . 1 95 64 64 GLU CB C 32.71 . 1 96 65 65 ALA CA C 51.33 . 1 97 65 65 ALA CB C 16.19 . 1 98 66 66 LYS CA C 53.77 . 1 99 66 66 LYS CB C 34.01 . 1 100 66 66 LYS CG C 26.16 . 1 101 66 66 LYS CD C 28.26 . 1 102 66 66 LYS CE C 42.8 . 1 103 68 68 LYS CA C 56.53 . 1 104 68 68 LYS CB C 37.05 . 1 105 68 68 LYS CG C 25.42 . 1 106 68 68 LYS CD C 29.54 . 1 107 68 68 LYS CE C 42.76 . 1 108 69 69 ASN CA C 53.38 . 1 109 69 69 ASN CB C 40.4 . 1 110 70 70 ASP CA C 52.79 . 1 111 70 70 ASP CB C 43.27 . 1 112 72 72 THR CA C 61.86 . 1 113 72 72 THR CB C 69.05 . 1 114 73 73 VAL CA C 61.48 . 1 115 73 73 VAL CB C 36.46 . 1 116 73 73 VAL CG2 C 22.07 . 1 117 74 74 GLN CA C 57.36 . 1 118 74 74 GLN CB C 26.4 . 1 119 77 77 LYS CA C 56.52 . 1 120 77 77 LYS CG C 24.41 . 1 121 78 78 TYR CA C 60.78 . 1 122 78 78 TYR CB C 36.52 . 1 123 79 79 PHE CA C 56.04 . 1 124 79 79 PHE CB C 39.33 . 1 125 80 80 THR CA C 62.79 . 1 126 80 80 THR CB C 71.97 . 1 127 80 80 THR CG2 C 22.06 . 1 128 82 82 ASP CA C 53.95 . 1 129 82 82 ASP CB C 41.02 . 1 130 83 83 GLU CA C 58.48 . 1 131 83 83 GLU CB C 29.6 . 1 132 83 83 GLU CG C 36.57 . 1 133 85 85 HIS CA C 56.78 . 1 134 85 85 HIS CB C 30.06 . 1 135 87 87 ILE CB C 43.76 . 1 136 87 87 ILE CG1 C 27.24 . 1 137 87 87 ILE CG2 C 18.51 . 1 138 87 87 ILE CD1 C 14.91 . 1 139 88 88 PHE CA C 57.95 . 1 140 88 88 PHE CB C 42.17 . 1 141 89 89 VAL CA C 58.77 . 1 142 89 89 VAL CB C 36.91 . 1 143 89 89 VAL CG1 C 23.76 . 1 144 89 89 VAL CG2 C 19.2 . 1 145 90 90 ARG CA C 53.37 . 1 146 90 90 ARG CD C 42.31 . 1 147 91 91 GLN CA C 59.79 . 1 148 91 91 GLN CB C 28.01 . 1 149 91 91 GLN CG C 33.69 . 1 150 92 92 SER CA C 60.32 . 1 151 92 92 SER CB C 62.46 . 1 152 93 93 GLN CA C 56.54 . 1 153 93 93 GLN CB C 31.23 . 1 154 93 93 GLN CG C 36.61 . 1 155 94 94 ILE CG1 C 25.64 . 1 156 94 94 ILE CD1 C 15.12 . 1 157 95 95 GLN CA C 53.19 . 1 158 95 95 GLN CG C 34.07 . 1 159 96 96 VAL CA C 62.68 . 1 160 96 96 VAL CB C 32.84 . 1 161 97 97 PHE CA C 56.03 . 1 162 97 97 PHE CB C 42.07 . 1 163 101 101 ALA CA C 52.78 . 9 164 101 101 ALA CB C 19.75 . 9 165 103 103 THR CA C 61.64 . 1 166 103 103 THR CB C 71.28 . 1 167 104 104 THR CA C 64.17 . 1 168 104 104 THR CB C 69.41 . 1 169 105 105 SER CA C 56.63 . 1 170 105 105 SER CB C 62.86 . 1 171 107 107 GLU CA C 55.74 . 1 172 107 107 GLU CG C 36.31 . 1 173 108 108 THR CA C 59.11 . 1 174 108 108 THR CB C 73.56 . 1 175 110 110 ASP CA C 54.1 . 1 176 110 110 ASP CB C 42.09 . 1 177 111 111 SER CA C 59.34 . 1 178 111 111 SER CB C 64.54 . 1 179 112 112 SER CA C 60.9 . 1 180 112 112 SER CB C 65.1 . 1 181 113 113 ALA CA C 53.27 . 9 182 113 113 ALA CB C 20.01 . 9 183 114 114 SER CA C 59.65 . 1 184 114 114 SER CB C 63.61 . 1 185 115 115 LYS CA C 56.98 . 9 186 115 115 LYS CB C 33.06 . 9 187 115 115 LYS CG C 21.02 . 9 188 115 115 LYS CE C 41.21 . 9 189 116 116 ILE CA C 61.49 . 1 190 116 116 ILE CG1 C 27.99 . 1 191 116 116 ILE CG2 C 18.51 . 1 192 116 116 ILE CD1 C 13.94 . 1 193 117 117 LEU CA C 55.53 . 1 194 117 117 LEU CB C 43.03 . 1 195 118 118 LYS CA C 56.98 . 9 196 118 118 LYS CB C 33.06 . 9 197 118 118 LYS CG C 21.02 . 9 198 118 118 LYS CE C 41.21 . 9 199 119 119 ARG CA C 55.22 . 1 200 119 119 ARG CB C 35.15 . 1 201 122 122 ALA CA C 53.27 . 9 202 122 122 ALA CB C 20.01 . 9 203 124 124 ALA CA C 53.44 . 1 204 124 124 ALA CB C 19.94 . 1 205 125 125 ALA CA C 53.09 . 9 206 125 125 ALA CB C 15.63 . 9 207 126 126 ALA CA C 53.09 . 9 208 126 126 ALA CB C 15.63 . 9 209 127 127 LYS CA C 56 . 1 210 127 127 LYS CB C 33.09 . 1 211 127 127 LYS CD C 30.27 . 1 212 128 128 THR CA C 60.64 . 9 213 128 128 THR CB C 69.82 . 9 214 128 128 THR CG2 C 21.77 . 9 215 129 129 SER CA C 57.87 . 1 216 129 129 SER CB C 64.58 . 1 217 130 130 LYS CA C 58.27 . 1 218 130 130 LYS CB C 32.01 . 1 219 130 130 LYS CD C 28.61 . 1 220 131 131 LEU CA C 56.15 . 1 221 131 131 LEU CB C 41.86 . 1 222 132 132 ARG CA C 56.57 . 1 223 132 132 ARG CB C 27.54 . 1 224 134 134 LEU CA C 54.71 . 1 225 134 134 LEU CB C 44.13 . 1 226 135 135 LYS CA C 54.57 . 1 227 135 135 LYS CB C 33.83 . 1 228 138 138 LYS CA C 55.92 . 1 229 138 138 LYS CB C 35.26 . 1 230 139 139 ALA CA C 50.67 . 1 231 139 139 ALA CB C 19.04 . 1 232 141 141 THR CA C 62.12 . 1 233 141 141 THR CB C 67.73 . 1 234 142 142 ALA CA C 49.39 . 1 235 142 142 ALA CB C 15.27 . 1 236 144 144 LYS CA C 56.73 . 1 237 144 144 LYS CE C 42.59 . 1 238 145 145 THR CA C 62.56 . 9 239 145 145 THR CB C 69.84 . 9 240 145 145 THR CG2 C 22.56 . 9 241 146 146 THR CA C 61.55 . 1 242 146 146 THR CB C 70.39 . 1 243 146 146 THR CG2 C 22.55 . 1 244 147 147 THR CA C 63.34 . 1 245 147 147 THR CB C 70.37 . 1 246 149 149 ARG CA C 53.88 . 9 247 149 149 ARG CG C 26.52 . 9 248 151 151 LYS CA C 56.57 . 9 249 151 151 LYS CB C 33.25 . 9 250 153 153 THR CA C 63.48 . 1 251 153 153 THR CB C 71.05 . 1 252 153 153 THR CG2 C 20.78 . 1 253 154 154 ARG CA C 53.88 . 9 254 154 154 ARG CG C 26.52 . 9 255 156 156 ALA CA C 52.78 . 9 256 156 156 ALA CB C 19.75 . 9 257 157 157 SER CA C 57.74 . 1 258 157 157 SER CB C 67.21 . 1 259 158 158 THR CA C 63.21 . 1 260 158 158 THR CB C 68.61 . 1 261 161 161 ALA CA C 52.18 . 9 262 161 161 ALA CB C 20.19 . 9 263 165 165 SER CA C 58.52 . 9 264 165 165 SER CB C 64.09 . 9 265 166 166 SER CA C 58.52 . 9 266 166 166 SER CB C 64.09 . 9 267 167 167 LEU CA C 55.17 . 9 268 167 167 LEU CB C 46.4 . 9 269 170 170 SER CA C 58.79 . 9 270 170 170 SER CB C 65.22 . 9 271 172 172 SER CA C 58.23 . 1 272 172 172 SER CB C 66.21 . 1 273 173 173 ALA CA C 52.78 . 9 274 173 173 ALA CB C 19.75 . 9 275 174 174 SER CA C 57.58 . 1 276 174 174 SER CB C 65.87 . 1 277 175 175 ALA CA C 52.78 . 9 278 175 175 ALA CB C 19.75 . 9 279 178 178 LEU CA C 55.17 . 9 280 178 178 LEU CB C 46.4 . 9 281 180 180 SER CA C 58.77 . 1 282 180 180 SER CB C 61.9 . 1 283 181 181 SER CA C 58.78 . 1 284 181 181 SER CB C 62.36 . 1 285 182 182 GLU CA C 56.86 . 1 286 182 182 GLU CG C 35.78 . 1 287 184 184 SER CA C 57.83 . 1 288 184 184 SER CB C 63.33 . 1 289 185 185 THR CA C 60.17 . 1 290 185 185 THR CB C 74.12 . 1 291 187 187 ALA CA C 52.18 . 9 292 187 187 ALA CB C 20.19 . 9 293 189 189 THR CA C 60.27 . 1 294 189 189 THR CB C 68.57 . 1 295 191 191 LEU CA C 54.33 . 1 296 191 191 LEU CB C 42.23 . 1 stop_ save_