data_28001 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Structural basis for client recognition and activity of hsp40 chaperones ; _BMRB_accession_number 28001 _BMRB_flat_file_name bmr28001.str _Entry_type original _Submission_date 2019-08-16 _Accession_date 2019-08-16 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Jiang Yajun . . 2 Rossi Paolo . . 3 Kalodimos Charalampos G. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 127 "15N chemical shifts" 127 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2019-11-07 original BMRB . stop_ loop_ _Related_BMRB_accession_number _Relationship 27998 'DnaJB1 SBD' 27999 'Sis1 SBD' 28000 'Ydj1 SBD' stop_ _Original_release_date 2019-08-19 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Structural basis for client recognition and activity of Hsp40 chaperones ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 31604242 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Jiang Yajun . . 2 Rossi Paolo . . 3 Kalodimos Charalampos G. . stop_ _Journal_abbreviation Science _Journal_volume 365 _Journal_issue 6459 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 1313 _Page_last 1319 _Year 2019 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name CbpA_SBD _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label CbpA_SBD $CbpA_SBD stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . loop_ _Biological_function Chaperone stop_ _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_CbpA_SBD _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common CbpA_SBD _Molecular_mass . _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 164 _Mol_residue_sequence ; TRGHDIEIEVAVFLEETLTE HKRTISYNLPVYNAFGMIEQ EIPKTLNVKIPAGVGNGQRI RLKGQGTPGENGGPNGDLWL VIHIAPHPLFDIVGQDLEIV VPVSPWEAALGAKVTVPTLK ESILLTIPPGSQAGQRLRVK GKGLVSKKQTGDLYAVLKIV MPPK ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 115 THR 2 116 ARG 3 117 GLY 4 118 HIS 5 119 ASP 6 120 ILE 7 121 GLU 8 122 ILE 9 123 GLU 10 124 VAL 11 125 ALA 12 126 VAL 13 127 PHE 14 128 LEU 15 129 GLU 16 130 GLU 17 131 THR 18 132 LEU 19 133 THR 20 134 GLU 21 135 HIS 22 136 LYS 23 137 ARG 24 138 THR 25 139 ILE 26 140 SER 27 141 TYR 28 142 ASN 29 143 LEU 30 144 PRO 31 145 VAL 32 146 TYR 33 147 ASN 34 148 ALA 35 149 PHE 36 150 GLY 37 151 MET 38 152 ILE 39 153 GLU 40 154 GLN 41 155 GLU 42 156 ILE 43 157 PRO 44 158 LYS 45 159 THR 46 160 LEU 47 161 ASN 48 162 VAL 49 163 LYS 50 164 ILE 51 165 PRO 52 166 ALA 53 167 GLY 54 168 VAL 55 169 GLY 56 170 ASN 57 171 GLY 58 172 GLN 59 173 ARG 60 174 ILE 61 175 ARG 62 176 LEU 63 177 LYS 64 178 GLY 65 179 GLN 66 180 GLY 67 181 THR 68 182 PRO 69 183 GLY 70 184 GLU 71 185 ASN 72 186 GLY 73 187 GLY 74 188 PRO 75 189 ASN 76 190 GLY 77 191 ASP 78 192 LEU 79 193 TRP 80 194 LEU 81 195 VAL 82 196 ILE 83 197 HIS 84 198 ILE 85 199 ALA 86 200 PRO 87 201 HIS 88 202 PRO 89 203 LEU 90 204 PHE 91 205 ASP 92 206 ILE 93 207 VAL 94 208 GLY 95 209 GLN 96 210 ASP 97 211 LEU 98 212 GLU 99 213 ILE 100 214 VAL 101 215 VAL 102 216 PRO 103 217 VAL 104 218 SER 105 219 PRO 106 220 TRP 107 221 GLU 108 222 ALA 109 223 ALA 110 224 LEU 111 225 GLY 112 226 ALA 113 227 LYS 114 228 VAL 115 229 THR 116 230 VAL 117 231 PRO 118 232 THR 119 233 LEU 120 234 LYS 121 235 GLU 122 236 SER 123 237 ILE 124 238 LEU 125 239 LEU 126 240 THR 127 241 ILE 128 242 PRO 129 243 PRO 130 244 GLY 131 245 SER 132 246 GLN 133 247 ALA 134 248 GLY 135 249 GLN 136 250 ARG 137 251 LEU 138 252 ARG 139 253 VAL 140 254 LYS 141 255 GLY 142 256 LYS 143 257 GLY 144 258 LEU 145 259 VAL 146 260 SER 147 261 LYS 148 262 LYS 149 263 GLN 150 264 THR 151 265 GLY 152 266 ASP 153 267 LEU 154 268 TYR 155 269 ALA 156 270 VAL 157 271 LEU 158 272 LYS 159 273 ILE 160 274 VAL 161 275 MET 162 276 PRO 163 277 PRO 164 278 LYS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $CbpA_SBD 'E. coli' 562 Bacteria . Escherichia coli stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $CbpA_SBD 'recombinant technology' . Escherichia coli . pet16b stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $CbpA_SBD 300 uM '[U-13C; U-15N; U-2H]' 'potassium phosphate' 20 mM 'natural abundance' 'potassium chloride' 75 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_PINE _Saveframe_category software _Name PINE _Version . loop_ _Vendor _Address _Electronic_address 'Bahrami, Markley, Assadi, and Eghbalnia' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCACB_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HNCO_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HNCA_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_1H-15N_NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ save_3D_HN(CO)CACB_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CACB' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.1 . M pH 7 . pH pressure 1 . atm temperature 283 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name CbpA_SBD _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 115 1 THR H H 7.990 0.00 . 2 115 1 THR N N 116.310 0.00 . 3 116 2 ARG H H 8.460 0.00 . 4 116 2 ARG N N 124.619 0.00 . 5 117 3 GLY H H 8.558 0.00 . 6 117 3 GLY N N 111.044 0.00 . 7 118 4 HIS H H 8.462 0.00 . 8 118 4 HIS N N 114.400 0.00 . 9 119 5 ASP H H 9.104 0.00 . 10 119 5 ASP N N 124.096 0.00 . 11 120 6 ILE H H 8.747 0.00 . 12 120 6 ILE N N 120.420 0.00 . 13 121 7 GLU H H 8.806 0.00 . 14 121 7 GLU N N 125.369 0.00 . 15 122 8 ILE H H 9.070 0.00 . 16 122 8 ILE N N 123.641 0.00 . 17 123 9 GLU H H 8.616 0.00 . 18 123 9 GLU N N 125.268 0.00 . 19 124 10 VAL H H 8.507 0.00 . 20 124 10 VAL N N 127.873 0.00 . 21 125 11 ALA H H 7.457 0.00 . 22 125 11 ALA N N 116.759 0.00 . 23 126 12 VAL H H 8.312 0.00 . 24 126 12 VAL N N 122.700 0.00 . 25 127 13 PHE H H 8.474 0.00 . 26 127 13 PHE N N 122.095 0.00 . 27 128 14 LEU H H 9.663 0.00 . 28 128 14 LEU N N 127.614 0.00 . 29 134 20 GLU H H 8.906 0.00 . 30 134 20 GLU N N 130.988 0.00 . 31 135 21 HIS H H 8.881 0.00 . 32 135 21 HIS N N 117.520 0.00 . 33 136 22 LYS H H 8.868 0.00 . 34 136 22 LYS N N 124.734 0.00 . 35 137 23 ARG H H 8.820 0.00 . 36 137 23 ARG N N 120.843 0.00 . 37 138 24 THR H H 8.937 0.00 . 38 138 24 THR N N 120.419 0.00 . 39 139 25 ILE H H 9.387 0.00 . 40 139 25 ILE N N 126.274 0.00 . 41 140 26 SER H H 8.604 0.00 . 42 140 26 SER N N 119.592 0.00 . 43 141 27 TYR H H 8.611 0.00 . 44 141 27 TYR N N 121.275 0.00 . 45 142 28 ASN H H 8.628 0.00 . 46 142 28 ASN N N 116.850 0.00 . 47 143 29 LEU H H 9.341 0.00 . 48 143 29 LEU N N 127.276 0.00 . 49 145 31 VAL H H 8.531 0.00 . 50 145 31 VAL N N 125.792 0.00 . 51 146 32 TYR H H 8.935 0.00 . 52 146 32 TYR N N 126.421 0.00 . 53 147 33 ASN H H 8.972 0.00 . 54 147 33 ASN N N 119.418 0.00 . 55 148 34 ALA H H 8.489 0.00 . 56 148 34 ALA N N 119.966 0.00 . 57 150 36 GLY H H 8.194 0.00 . 58 150 36 GLY N N 107.185 0.00 . 59 152 38 ILE H H 8.864 0.00 . 60 152 38 ILE N N 130.391 0.00 . 61 153 39 GLU H H 9.118 0.00 . 62 153 39 GLU N N 128.653 0.00 . 63 154 40 GLN H H 7.601 0.00 . 64 154 40 GLN N N 115.428 0.00 . 65 155 41 GLU H H 8.529 0.00 . 66 155 41 GLU N N 118.887 0.00 . 67 156 42 ILE H H 9.058 0.00 . 68 156 42 ILE N N 124.356 0.00 . 69 158 44 LYS H H 8.914 0.00 . 70 158 44 LYS N N 124.008 0.00 . 71 159 45 THR H H 8.049 0.00 . 72 159 45 THR N N 112.646 0.00 . 73 160 46 LEU H H 8.650 0.00 . 74 160 46 LEU N N 124.375 0.00 . 75 162 48 VAL H H 8.849 0.00 . 76 162 48 VAL N N 119.411 0.00 . 77 163 49 LYS H H 8.347 0.00 . 78 163 49 LYS N N 123.026 0.00 . 79 164 50 ILE H H 9.009 0.00 . 80 164 50 ILE N N 128.586 0.00 . 81 167 53 GLY H H 8.970 0.00 . 82 167 53 GLY N N 111.369 0.00 . 83 168 54 VAL H H 7.742 0.00 . 84 168 54 VAL N N 121.309 0.00 . 85 169 55 GLY H H 7.890 0.00 . 86 169 55 GLY N N 106.781 0.00 . 87 170 56 ASN H H 8.675 0.00 . 88 170 56 ASN N N 116.612 0.00 . 89 171 57 GLY H H 9.271 0.00 . 90 171 57 GLY N N 116.486 0.00 . 91 172 58 GLN H H 7.761 0.00 . 92 172 58 GLN N N 122.215 0.00 . 93 173 59 ARG H H 8.521 0.00 . 94 173 59 ARG N N 123.439 0.00 . 95 174 60 ILE H H 9.698 0.00 . 96 174 60 ILE N N 126.261 0.00 . 97 175 61 ARG H H 8.654 0.00 . 98 175 61 ARG N N 128.834 0.00 . 99 176 62 LEU H H 9.413 0.00 . 100 176 62 LEU N N 131.379 0.00 . 101 177 63 LYS H H 8.481 0.00 . 102 177 63 LYS N N 125.930 0.00 . 103 178 64 GLY H H 9.745 0.00 . 104 178 64 GLY N N 113.737 0.00 . 105 179 65 GLN H H 6.761 0.00 . 106 179 65 GLN N N 114.301 0.00 . 107 180 66 GLY H H 7.938 0.00 . 108 180 66 GLY N N 107.800 0.00 . 109 183 69 GLY H H 8.050 0.00 . 110 183 69 GLY N N 109.460 0.00 . 111 184 70 GLU H H 8.180 0.00 . 112 184 70 GLU N N 117.065 0.00 . 113 185 71 ASN H H 9.597 0.00 . 114 185 71 ASN N N 120.151 0.00 . 115 186 72 GLY H H 8.575 0.00 . 116 186 72 GLY N N 103.831 0.00 . 117 187 73 GLY H H 7.602 0.00 . 118 187 73 GLY N N 107.842 0.00 . 119 189 75 ASN H H 8.681 0.00 . 120 189 75 ASN N N 118.625 0.00 . 121 190 76 GLY H H 8.307 0.00 . 122 190 76 GLY N N 103.128 0.00 . 123 191 77 ASP H H 9.083 0.00 . 124 191 77 ASP N N 118.294 0.00 . 125 192 78 LEU H H 7.452 0.00 . 126 192 78 LEU N N 121.139 0.00 . 127 193 79 TRP H H 9.393 0.00 . 128 193 79 TRP N N 125.843 0.00 . 129 194 80 LEU H H 9.393 0.00 . 130 194 80 LEU N N 122.290 0.00 . 131 195 81 VAL H H 8.931 0.00 . 132 195 81 VAL N N 123.600 0.00 . 133 196 82 ILE H H 9.093 0.00 . 134 196 82 ILE N N 117.881 0.00 . 135 197 83 HIS H H 9.238 0.00 . 136 197 83 HIS N N 118.738 0.00 . 137 198 84 ILE H H 8.592 0.00 . 138 198 84 ILE N N 122.487 0.00 . 139 204 90 PHE H H 8.481 0.00 . 140 204 90 PHE N N 127.764 0.00 . 141 205 91 ASP H H 8.348 0.00 . 142 205 91 ASP N N 120.384 0.00 . 143 206 92 ILE H H 8.775 0.00 . 144 206 92 ILE N N 123.298 0.00 . 145 207 93 VAL H H 8.364 0.00 . 146 207 93 VAL N N 120.849 0.00 . 147 208 94 GLY H H 8.599 0.00 . 148 208 94 GLY N N 111.413 0.00 . 149 209 95 GLN H H 7.813 0.00 . 150 209 95 GLN N N 118.777 0.00 . 151 210 96 ASP H H 8.635 0.00 . 152 210 96 ASP N N 122.731 0.00 . 153 211 97 LEU H H 8.579 0.00 . 154 211 97 LEU N N 122.538 0.00 . 155 212 98 GLU H H 8.540 0.00 . 156 212 98 GLU N N 124.797 0.00 . 157 213 99 ILE H H 8.767 0.00 . 158 213 99 ILE N N 124.136 0.00 . 159 214 100 VAL H H 9.054 0.00 . 160 214 100 VAL N N 119.709 0.00 . 161 215 101 VAL H H 9.011 0.00 . 162 215 101 VAL N N 127.839 0.00 . 163 217 103 VAL H H 8.584 0.00 . 164 217 103 VAL N N 130.352 0.00 . 165 218 104 SER H H 8.098 0.00 . 166 218 104 SER N N 119.027 0.00 . 167 220 106 TRP H H 9.630 0.00 . 168 220 106 TRP N N 130.432 0.00 . 169 221 107 GLU H H 8.700 0.00 . 170 221 107 GLU N N 128.193 0.00 . 171 222 108 ALA H H 8.600 0.00 . 172 222 108 ALA N N 121.650 0.00 . 173 223 109 ALA H H 8.595 0.00 . 174 223 109 ALA N N 133.424 0.00 . 175 226 112 ALA H H 8.177 0.00 . 176 226 112 ALA N N 118.868 0.00 . 177 227 113 LYS H H 8.388 0.00 . 178 227 113 LYS N N 119.315 0.00 . 179 228 114 VAL H H 8.950 0.00 . 180 228 114 VAL N N 126.593 0.00 . 181 229 115 THR H H 7.665 0.00 . 182 229 115 THR N N 105.266 0.00 . 183 230 116 VAL H H 7.214 0.00 . 184 230 116 VAL N N 122.491 0.00 . 185 233 119 LEU H H 8.214 0.00 . 186 233 119 LEU N N 118.793 0.00 . 187 234 120 LYS H H 8.644 0.00 . 188 234 120 LYS N N 121.014 0.00 . 189 235 121 GLU H H 8.643 0.00 . 190 235 121 GLU N N 123.405 0.00 . 191 236 122 SER H H 8.777 0.00 . 192 236 122 SER N N 119.320 0.00 . 193 237 123 ILE H H 9.411 0.00 . 194 237 123 ILE N N 128.950 0.00 . 195 238 124 LEU H H 9.404 0.00 . 196 238 124 LEU N N 129.949 0.00 . 197 239 125 LEU H H 7.067 0.00 . 198 239 125 LEU N N 118.666 0.00 . 199 240 126 THR H H 7.353 0.00 . 200 240 126 THR N N 106.063 0.00 . 201 241 127 ILE H H 8.658 0.00 . 202 241 127 ILE N N 121.125 0.00 . 203 245 131 SER H H 8.984 0.00 . 204 245 131 SER N N 122.269 0.00 . 205 246 132 GLN H H 8.801 0.00 . 206 246 132 GLN N N 120.801 0.00 . 207 247 133 ALA H H 8.507 0.00 . 208 247 133 ALA N N 120.771 0.00 . 209 248 134 GLY H H 9.300 0.00 . 210 248 134 GLY N N 109.703 0.00 . 211 249 135 GLN H H 8.563 0.00 . 212 249 135 GLN N N 117.242 0.00 . 213 250 136 ARG H H 8.306 0.00 . 214 250 136 ARG N N 120.665 0.00 . 215 251 137 LEU H H 8.971 0.00 . 216 251 137 LEU N N 124.408 0.00 . 217 252 138 ARG H H 8.804 0.00 . 218 252 138 ARG N N 124.127 0.00 . 219 253 139 VAL H H 8.691 0.00 . 220 253 139 VAL N N 122.760 0.00 . 221 254 140 LYS H H 9.077 0.00 . 222 254 140 LYS N N 123.021 0.00 . 223 255 141 GLY H H 9.685 0.00 . 224 255 141 GLY N N 111.439 0.00 . 225 261 147 LYS H H 9.060 0.00 . 226 261 147 LYS N N 117.305 0.00 . 227 262 148 LYS H H 8.197 0.00 . 228 262 148 LYS N N 115.185 0.00 . 229 263 149 GLN H H 7.653 0.00 . 230 263 149 GLN N N 116.873 0.00 . 231 264 150 THR H H 8.666 0.00 . 232 264 150 THR N N 119.641 0.00 . 233 265 151 GLY H H 7.544 0.00 . 234 265 151 GLY N N 107.264 0.00 . 235 266 152 ASP H H 9.441 0.00 . 236 266 152 ASP N N 118.051 0.00 . 237 267 153 LEU H H 9.563 0.00 . 238 267 153 LEU N N 131.731 0.00 . 239 268 154 TYR H H 9.629 0.00 . 240 268 154 TYR N N 123.575 0.00 . 241 269 155 ALA H H 9.083 0.00 . 242 269 155 ALA N N 126.597 0.00 . 243 270 156 VAL H H 9.236 0.00 . 244 270 156 VAL N N 124.873 0.00 . 245 271 157 LEU H H 8.125 0.00 . 246 271 157 LEU N N 117.582 0.00 . 247 272 158 LYS H H 8.459 0.00 . 248 272 158 LYS N N 126.068 0.00 . 249 273 159 ILE H H 8.865 0.00 . 250 273 159 ILE N N 129.307 0.00 . 251 274 160 VAL H H 8.646 0.00 . 252 274 160 VAL N N 126.267 0.00 . 253 275 161 MET H H 8.357 0.00 . 254 275 161 MET N N 115.956 0.00 . stop_ save_