data_28025 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; human_CIRBP_138-172 ; _BMRB_accession_number 28025 _BMRB_flat_file_name bmr28025.str _Entry_type original _Submission_date 2019-10-04 _Accession_date 2019-10-04 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bourgeois Benjamin . . 2 Madl Tobias . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 35 "15N chemical shifts" 35 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-04-21 update BMRB 'update entry citation' 2020-04-03 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 28026 'human TNPO1 328-381' 28027 'human CIRBP 38-137' stop_ _Original_release_date 2019-10-04 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Nonclassical nuclear localization signals mediate nuclear import of CIRBP ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 32234784 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bourgeois Benjamin . . 2 Hutten Saskia . . 3 Gottschalk Benjamin . . 4 Hofweber Mario . . 5 Richter Gesa . . 6 Sternat Julia . . 7 Abou-Ajram Claudia . . 8 Gobl Christoph . . 9 Leitinger Gerd . . 10 Graier Wolfgang F. . 11 Dormann Dorothee . . 12 Madl Tobias . . stop_ _Journal_abbreviation 'Proc. Natl. Acad. Sci. U.S.A.' _Journal_name_full 'Proceedings of the National Academy of Sciences of the United States of America' _Journal_volume 117 _Journal_issue 15 _Journal_ISSN 1091-6490 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 8503 _Page_last 8514 _Year 2020 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name human_CIRBP_138-172 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label CIRBP_138-172 $CIRBP stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_CIRBP _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common CIRBP _Molecular_mass . _Mol_thiol_state unknown _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 38 _Mol_residue_sequence ; GAMSRDYYSSRSQSGGYSDR SSGGSYRDSYDSYATHNE ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 135 GLY 2 136 ALA 3 137 MET 4 138 SER 5 139 ARG 6 140 ASP 7 141 TYR 8 142 TYR 9 143 SER 10 144 SER 11 145 ARG 12 146 SER 13 147 GLN 14 148 SER 15 149 GLY 16 150 GLY 17 151 TYR 18 152 SER 19 153 ASP 20 154 ARG 21 155 SER 22 156 SER 23 157 GLY 24 158 GLY 25 159 SER 26 160 TYR 27 161 ARG 28 162 ASP 29 163 SER 30 164 TYR 31 165 ASP 32 166 SER 33 167 TYR 34 168 ALA 35 169 THR 36 170 HIS 37 171 ASN 38 172 GLU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Gene_mnemonic _Details $CIRBP Human 9606 Eukaryota Metazoa Homo sapiens CIRBP 'fragment 138-172' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $CIRBP 'recombinant technology' . Escherichia coli . pETM-11 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $CIRBP 500 uM '[U-99% 13C; U-99% 15N]' D2O 10 % 'natural abundance' TRIS 50 mM 'natural abundance' 'sodium chloride' 150 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CCPN _Saveframe_category software _Name CCPN _Version . loop_ _Vendor _Address _Electronic_address CCPN . . stop_ loop_ _Task collection 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCACB_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HNCANNH_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCANNH' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.15 . M pH 6.7 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water H 1 protons ppm 4.673 na indirect . . . 1 '[15N] ammonium chloride' N 15 nitrogen ppm 117 na indirect . . . 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCACB' '3D HNCANNH' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name CIRBP_138-172 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 138 4 SER H H 8.11645 0.0014 1 2 138 4 SER N N 115.67378 0.02084 1 3 139 5 ARG H H 8.34229 3.54E-04 1 4 139 5 ARG N N 122.4270 0.02569 1 5 140 6 ASP H H 8.14288 2.54E-05 1 6 140 6 ASP N N 120.5893 0.09324 1 7 141 7 TYR H H 7.93879 6.09E-04 1 8 141 7 TYR N N 120.70369 0.12127 1 9 142 8 TYR H H 7.96283 0.0169 1 10 142 8 TYR N N 120.403 0.00914 1 11 143 9 SER H H 116.95709 0.32777 1 12 143 9 SER N N 8.00193 0.0165 1 13 144 10 SER H H 8.14418 8.80E-04 1 14 144 10 SER N N 117.1842 0.32341 1 15 145 11 ARG H H 8.10878 0.02509 1 16 145 11 ARG N N 122.26617 0.00133 1 17 146 12 SER H H 8.12392 0.02277 1 18 146 12 SER N N 116.20576 0.01433 1 19 147 13 GLN H H 8.2978 6.30E-04 1 20 147 13 GLN N N 122.00058 0.00375 1 21 148 14 SER H H 8.23283 4.58E-04 1 22 148 14 SER N N 116.2579 0.00578 1 23 149 15 GLY H H 8.30943 4.61E-04 1 24 149 15 GLY N N 110.74106 0.00723 1 25 150 16 GLY H H 8.12519 6.54E-04 1 26 150 16 GLY N N 108.49657 0.01149 1 27 151 17 TYR H H 8.05821 0.04838 1 28 151 17 TYR N N 120.07194 0.00933 1 29 152 18 SER H H 8.08104 0.04171 1 30 152 18 SER N N 117.30174 0.0083 1 31 153 19 ASP H H 8.18269 0.03745 1 32 153 19 ASP N N 122.29548 0.01203 1 33 154 20 ARG H H 8.19104 0.01122 1 34 154 20 ARG N N 121.15632 0.00513 1 35 155 21 SER H H 8.2468 0.04199 1 36 155 21 SER N N 116.48391 0.01382 1 37 156 22 SER H H 8.2613 0.03328 1 38 156 22 SER N N 117.35983 0.00473 1 39 157 23 GLY H H 8.28444 0.03275 1 40 157 23 GLY N N 110.50417 0.00595 1 41 158 24 GLY H H 8.13935 0.01211 1 42 158 24 GLY N N 108.61015 0.01291 1 43 159 25 SER H H 8.12782 0.01236 1 44 159 25 SER N N 115.59862 0.01733 1 45 160 26 TYR H H 8.14809 0.01076 1 46 160 26 TYR N N 122.4321 0.1276 1 47 161 27 ARG H H 7.966 4.14E-04 1 48 161 27 ARG N N 122.52438 0.03233 1 49 162 28 ASP H H 8.13791 3.70E-04 1 50 162 28 ASP N N 121.04119 0.00341 1 51 163 29 SER H H 8.0088 7.71E-04 1 52 163 29 SER N N 115.10864 0.01644 1 53 164 30 TYR H H 8.12212 0.01194 1 54 164 30 TYR N N 121.99722 0.02129 1 55 165 31 ASP H H 8.10498 2.20E-04 1 56 165 31 ASP N N 121.41955 0.00315 1 57 166 32 SER H H 8.03127 0.00285 1 58 166 32 SER N N 116.24957 0.01524 1 59 167 33 TYR H H 8.03077 0.00285 1 60 167 33 TYR N N 121.44032 0.00578 1 61 168 34 ALA H H 7.87468 0.01556 1 62 168 34 ALA N N 124.34292 0.04546 1 63 169 35 THR H H 7.88779 0.02448 1 64 169 35 THR N N 112.51834 0.02261 1 65 170 36 HIS H H 8.31063 0.01887 1 66 170 36 HIS N N 120.65221 0.08828 1 67 171 37 ASN H H 8.39358 0.01625 1 68 171 37 ASN N N 120.95473 0.02519 1 69 172 38 GLU H H 7.97736 0.00757 1 70 172 38 GLU N N 126.09981 0.00464 1 stop_ save_