data_28026 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; TNPO1_328-381 ; _BMRB_accession_number 28026 _BMRB_flat_file_name bmr28026.str _Entry_type original _Submission_date 2019-10-04 _Accession_date 2019-10-04 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 bourgeois benjamin . . 2 madl tobias . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 45 "15N chemical shifts" 46 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-04-21 update BMRB 'update entry citation' 2020-04-03 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 28025 'human CIRBP 138-172' 28027 'human CIRBP 38-137' stop_ _Original_release_date 2019-10-04 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Nonclassical nuclear localization signals mediate nuclear import of CIRBP ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 32234784 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bourgeois Benjamin . . 2 Hutten Saskia . . 3 Gottschalk Benjamin . . 4 Hofweber Mario . . 5 Richter Gesa . . 6 Sternat Julia . . 7 Abou-Ajram Claudia . . 8 Gobl Christoph . . 9 Leitinger Gerd . . 10 Graier Wolfgang F. . 11 Dormann Dorothee . . 12 Madl Tobias . . stop_ _Journal_abbreviation 'Proc. Natl. Acad. Sci. U.S.A.' _Journal_name_full 'Proceedings of the National Academy of Sciences of the United States of America' _Journal_volume 117 _Journal_issue 15 _Journal_ISSN 1091-6490 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 8503 _Page_last 8514 _Year 2020 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name human_TNPO1_328-381 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label TNPO1_328-381 $TNPO1 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_TNPO1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common TNPO1 _Molecular_mass . _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 57 _Mol_residue_sequence ; GAMGDVEEDETIPDSEQDIR PRFHRSRTVAQQHDEDGIEE EDDDDDEIDDDDTISDW ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 325 GLY 2 326 ALA 3 327 MET 4 328 GLY 5 329 ASP 6 330 VAL 7 331 GLU 8 332 GLU 9 333 ASP 10 334 GLU 11 335 THR 12 336 ILE 13 337 PRO 14 338 ASP 15 339 SER 16 340 GLU 17 341 GLN 18 342 ASP 19 343 ILE 20 344 ARG 21 345 PRO 22 346 ARG 23 347 PHE 24 348 HIS 25 349 ARG 26 350 SER 27 351 ARG 28 352 THR 29 353 VAL 30 354 ALA 31 355 GLN 32 356 GLN 33 357 HIS 34 358 ASP 35 359 GLU 36 360 ASP 37 361 GLY 38 362 ILE 39 363 GLU 40 364 GLU 41 365 GLU 42 366 ASP 43 367 ASP 44 368 ASP 45 369 ASP 46 370 ASP 47 371 GLU 48 372 ILE 49 373 ASP 50 374 ASP 51 375 ASP 52 376 ASP 53 377 THR 54 378 ILE 55 379 SER 56 380 ASP 57 381 TRP stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $TNPO1 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $TNPO1 'recombinant technology' . Escherichia coli . pETM-11 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $TNPO1 500 uM '[U-99% 13C; U-99% 15N]' D2O 10 % 'natural abundance' TRIS 50 mM 'natural abundance' 'sodium chloride' 150 mM 'natural abundance' TCEP 2 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CCPN _Saveframe_category software _Name CCPN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . CCPN . . 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task collection 'peak picking' processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer GE _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCACB_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HN(CA)NNH_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CA)NNH' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.15 . M pH 6.7 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water H 1 protons ppm 4.708 na indirect . . . 1 'ammonium chloride' N 15 nitrogen ppm 117 na indirect . . . 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCACB' '3D HN(CA)NNH' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name TNPO1_328-381 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 328 4 GLY N N 110.288 0.079 . 2 329 5 ASP H H 8.174 0.005 . 3 329 5 ASP N N 120.811 0.014 . 4 330 6 VAL H H 7.987 0.005 . 5 330 6 VAL N N 119.449 0.025 . 6 331 7 GLU H H 8.428 0.002 . 7 331 7 GLU N N 124.771 0.006 . 8 332 8 GLU H H 8.356 0.005 . 9 332 8 GLU N N 122.231 0.010 . 10 334 10 GLU H H 8.366 0.005 . 11 334 10 GLU N N 121.973 0.007 . 12 335 11 THR H H 8.249 0.002 . 13 335 11 THR N N 116.440 0.058 . 14 336 12 ILE H H 8.197 0.003 . 15 336 12 ILE N N 125.861 0.015 . 16 338 14 ASP H H 8.399 0.004 . 17 338 14 ASP N N 120.995 0.011 . 18 339 15 SER H H 8.182 0.002 . 19 339 15 SER N N 115.517 0.031 . 20 340 16 GLU H H 8.358 0.001 . 21 340 16 GLU N N 122.203 0.029 . 22 341 17 GLN H H 8.070 0.003 . 23 341 17 GLN N N 119.748 0.007 . 24 342 18 ASP H H 8.225 0.003 . 25 342 18 ASP N N 121.099 0.055 . 26 343 19 ILE H H 7.910 0.003 . 27 343 19 ILE N N 120.265 0.005 . 28 344 20 ARG H H 8.185 0.003 . 29 344 20 ARG N N 125.572 0.020 . 30 346 22 ARG H H 8.269 0.003 . 31 346 22 ARG N N 120.806 0.046 . 32 347 23 PHE H H 8.111 0.002 . 33 347 23 PHE N N 120.482 0.003 . 34 349 25 ARG H H 8.292 0.006 . 35 349 25 ARG N N 123.000 0.009 . 36 350 26 SER H H 8.415 0.001 . 37 350 26 SER N N 117.426 0.010 . 38 351 27 ARG H H 8.392 0.005 . 39 351 27 ARG N N 123.124 0.018 . 40 352 28 THR H H 8.186 0.003 . 41 352 28 THR N N 115.508 0.027 . 42 353 29 VAL H H 8.053 0.003 . 43 353 29 VAL N N 122.200 0.025 . 44 354 30 ALA H H 8.262 0.002 . 45 354 30 ALA N N 126.923 0.009 . 46 355 31 GLN H H 8.171 0.002 . 47 355 31 GLN N N 119.197 0.002 . 48 356 32 GLN H H 8.285 0.005 . 49 356 32 GLN N N 121.146 0.012 . 50 357 33 HIS H H 8.409 0.006 . 51 357 33 HIS N N 119.779 0.019 . 52 358 34 ASP H H 8.362 0.004 . 53 358 34 ASP N N 121.913 0.037 . 54 359 35 GLU H H 8.457 0.002 . 55 359 35 GLU N N 120.775 0.040 . 56 360 36 ASP H H 8.237 0.003 . 57 360 36 ASP N N 120.560 0.018 . 58 361 37 GLY H H 8.181 0.001 . 59 361 37 GLY N N 109.012 0.019 . 60 362 38 ILE H H 7.895 0.002 . 61 362 38 ILE N N 119.934 0.037 . 62 363 39 GLU H H 8.455 0.002 . 63 363 39 GLU N N 125.353 0.013 . 64 364 40 GLU H H 8.357 0.003 . 65 364 40 GLU N N 122.566 0.010 . 66 365 41 GLU H H 8.400 0.002 . 67 365 41 GLU N N 122.285 0.004 . 68 366 42 ASP H H 8.361 0.005 . 69 366 42 ASP N N 122.146 0.032 . 70 371 47 GLU H H 8.225 0.002 . 71 371 47 GLU N N 121.118 0.028 . 72 372 48 ILE H H 8.080 0.008 . 73 372 48 ILE N N 122.234 0.037 . 74 373 49 ASP H H 8.365 0.004 . 75 373 49 ASP N N 125.339 0.020 . 76 374 50 ASP H H 8.246 0.003 . 77 374 50 ASP N N 121.867 0.060 . 78 375 51 ASP H H 8.263 0.010 . 79 375 51 ASP N N 120.876 0.024 . 80 376 52 ASP H H 8.280 0.002 . 81 376 52 ASP N N 121.395 0.019 . 82 377 53 THR H H 8.073 0.002 . 83 377 53 THR N N 114.462 0.018 . 84 378 54 ILE H H 7.989 0.003 . 85 378 54 ILE N N 123.423 0.032 . 86 379 55 SER H H 8.222 0.003 . 87 379 55 SER N N 120.063 0.033 . 88 380 56 ASP H H 8.034 0.002 . 89 380 56 ASP N N 122.797 0.080 . 90 381 57 TRP H H 7.498 0.002 . 91 381 57 TRP N N 125.456 0.021 . stop_ save_