data_50127 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 50127 _Entry.Title ; Influenza A virus M2 cytoplasmic tail at pH 5.5 ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2019-12-13 _Entry.Accession_date 2019-12-13 _Entry.Last_release_date 2019-12-13 _Entry.Original_release_date 2019-12-13 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.6.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Jolyon Claridge . K. . 0000-0003-2244-8642 50127 2 Jason Schnell . R. . 0000-0002-8204-7608 50127 stop_ loop_ _Entry_src.ID _Entry_src.Project_name _Entry_src.Organization_full_name _Entry_src.Organization_initials _Entry_src.Entry_ID 1 . 'Oxford University' . 50127 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 50127 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '13C chemical shifts' 97 50127 '15N chemical shifts' 36 50127 '1H chemical shifts' 36 50127 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2020-06-09 . original BMRB . 50127 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 50127 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID 32157574 _Citation.DOI . _Citation.Full_citation . _Citation.Title ; pH-dependent Secondary Structure Propensity of the Influenza A Virus M2 Cytoplasmic Tail ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Biomol. NMR Assignments' _Citation.Journal_name_full . _Citation.Journal_volume 14 _Citation.Journal_issue 1 _Citation.Journal_ASTM . _Citation.Journal_ISSN 1874-270X _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 157 _Citation.Page_last 161 _Citation.Year 2020 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Jolyon Claridge . K. . . 50127 1 2 Faiz Mohd-Kipli . . . . 50127 1 3 Andrei Florea . . . . 50127 1 4 Thomas Gate . . . . 50127 1 5 Jason Schnell . . . . 50127 1 stop_ loop_ _Citation_keyword.Keyword _Citation_keyword.Entry_ID _Citation_keyword.Citation_ID 'Influenza, matrix protein 2, M2' 50127 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 50127 _Assembly.ID 1 _Assembly.Name 'Influenza A virus matrix 2 protein' _Assembly.BMRB_code . _Assembly.Number_of_components 1 _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass 6740 _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'Influenza A virus matrix 2 protein' 1 $entity_1 . . yes native no no . . . 50127 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 50127 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID 1 _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; GPGSDRLFFKCIYRRFKYGL KRGPSTEGVPESMREEYQQE QQSAVDVDDGHFVNIELE ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq 40-97 _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 58 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment 'cytoplasmic tail' _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 yes Uniprot J9V8B5 . 'Matrix protein 2' . . . . . . . . . . . . . . 50127 1 stop_ loop_ _Entity_biological_function.Biological_function _Entity_biological_function.Entry_ID _Entity_biological_function.Entity_ID 'Several roles in the life cycle of Influenza A virus' 50127 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 40 GLY . 50127 1 2 41 PRO . 50127 1 3 42 GLY . 50127 1 4 43 SER . 50127 1 5 44 ASP . 50127 1 6 45 ARG . 50127 1 7 46 LEU . 50127 1 8 47 PHE . 50127 1 9 48 PHE . 50127 1 10 49 LYS . 50127 1 11 50 CYS . 50127 1 12 51 ILE . 50127 1 13 52 TYR . 50127 1 14 53 ARG . 50127 1 15 54 ARG . 50127 1 16 55 PHE . 50127 1 17 56 LYS . 50127 1 18 57 TYR . 50127 1 19 58 GLY . 50127 1 20 59 LEU . 50127 1 21 60 LYS . 50127 1 22 61 ARG . 50127 1 23 62 GLY . 50127 1 24 63 PRO . 50127 1 25 64 SER . 50127 1 26 65 THR . 50127 1 27 66 GLU . 50127 1 28 67 GLY . 50127 1 29 68 VAL . 50127 1 30 69 PRO . 50127 1 31 70 GLU . 50127 1 32 71 SER . 50127 1 33 72 MET . 50127 1 34 73 ARG . 50127 1 35 74 GLU . 50127 1 36 75 GLU . 50127 1 37 76 TYR . 50127 1 38 77 GLN . 50127 1 39 78 GLN . 50127 1 40 79 GLU . 50127 1 41 80 GLN . 50127 1 42 81 GLN . 50127 1 43 82 SER . 50127 1 44 83 ALA . 50127 1 45 84 VAL . 50127 1 46 85 ASP . 50127 1 47 86 VAL . 50127 1 48 87 ASP . 50127 1 49 88 ASP . 50127 1 50 89 GLY . 50127 1 51 90 HIS . 50127 1 52 91 PHE . 50127 1 53 92 VAL . 50127 1 54 93 ASN . 50127 1 55 94 ILE . 50127 1 56 95 GLU . 50127 1 57 96 LEU . 50127 1 58 97 GLU . 50127 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . GLY 1 1 50127 1 . PRO 2 2 50127 1 . GLY 3 3 50127 1 . SER 4 4 50127 1 . ASP 5 5 50127 1 . ARG 6 6 50127 1 . LEU 7 7 50127 1 . PHE 8 8 50127 1 . PHE 9 9 50127 1 . LYS 10 10 50127 1 . CYS 11 11 50127 1 . ILE 12 12 50127 1 . TYR 13 13 50127 1 . ARG 14 14 50127 1 . ARG 15 15 50127 1 . PHE 16 16 50127 1 . LYS 17 17 50127 1 . TYR 18 18 50127 1 . GLY 19 19 50127 1 . LEU 20 20 50127 1 . LYS 21 21 50127 1 . ARG 22 22 50127 1 . GLY 23 23 50127 1 . PRO 24 24 50127 1 . SER 25 25 50127 1 . THR 26 26 50127 1 . GLU 27 27 50127 1 . GLY 28 28 50127 1 . VAL 29 29 50127 1 . PRO 30 30 50127 1 . GLU 31 31 50127 1 . SER 32 32 50127 1 . MET 33 33 50127 1 . ARG 34 34 50127 1 . GLU 35 35 50127 1 . GLU 36 36 50127 1 . TYR 37 37 50127 1 . GLN 38 38 50127 1 . GLN 39 39 50127 1 . GLU 40 40 50127 1 . GLN 41 41 50127 1 . GLN 42 42 50127 1 . SER 43 43 50127 1 . ALA 44 44 50127 1 . VAL 45 45 50127 1 . ASP 46 46 50127 1 . VAL 47 47 50127 1 . ASP 48 48 50127 1 . ASP 49 49 50127 1 . GLY 50 50 50127 1 . HIS 51 51 50127 1 . PHE 52 52 50127 1 . VAL 53 53 50127 1 . ASN 54 54 50127 1 . ILE 55 55 50127 1 . GLU 56 56 50127 1 . LEU 57 57 50127 1 . GLU 58 58 50127 1 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 50127 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 11320 organism . 'Influenza A' 'Influenza A' . . Viruses . Alphainfluenzavirus 'Influenza A' H1N1 'England 2009 250' . . . . . . . . . 'matrix protein 2' . 50127 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 50127 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli 'BL21 pLysS' . . plasmid . . pMALc . . . 50127 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 50127 _Sample.ID 1 _Sample.Name . _Sample.Type solution _Sample.Sub_type . _Sample.Details 'M2 cytoplasmic tail; 20 mM NaPO4, 20 mM Na acetate, 137 mM NaCl, 2.7 mM KCl, and pH 5.5' _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '95% H2O/5% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'Influenza A virus matrix protein 2' '[U-99% 13C; U-99% 15N]' . . 1 $entity_1 . . 0.225 . . mM . . . . 50127 1 2 NaPO4 'natural abundance' . . . . . . 20 . . mM . . . . 50127 1 3 'Na acetate' 'natural abundance' . . . . . . 20 . . mM . . . . 50127 1 4 NaCl 'natural abundance' . . . . . . 137 . . mM . . . . 50127 1 5 KCl 'natural abundance' . . . . . . 2.7 . . mM . . . . 50127 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 50127 _Sample_condition_list.ID 1 _Sample_condition_list.Name . _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 0.180 . M 50127 1 pH 5.5 . pH 50127 1 pressure 1 . atm 50127 1 temperature 310 . K 50127 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 50127 _Software.ID 1 _Software.Type . _Software.Name NMRPipe _Software.Version 8.7 _Software.DOI . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . 50127 1 stop_ loop_ _Task.Software_module _Task.Task _Task.Entry_ID _Task.Software_ID . processing 50127 1 stop_ save_ save_software_2 _Software.Sf_category software _Software.Sf_framecode software_2 _Software.Entry_ID 50127 _Software.ID 2 _Software.Type . _Software.Name CcpNMR _Software.Version 2 _Software.DOI . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID CCPN . . 50127 2 stop_ loop_ _Task.Software_module _Task.Task _Task.Entry_ID _Task.Software_ID . 'chemical shift assignment' 50127 2 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 50127 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name . _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model Avance _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 50127 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '3D HNCO' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . 50127 1 2 '3D HNCA' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . 50127 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 50127 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name . _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID C 13 DSS 'methyl protons' . . . . ppm 0 internal indirect 0.251449530 . . . . . 50127 1 H 1 DSS 'methyl protons' . . . . ppm 0 internal direct 1 . . . . . 50127 1 N 15 DSS 'methyl protons' . . . . ppm 0 internal indirect 0.101329118 . . . . . 50127 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_2 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_2 _Assigned_chem_shift_list.Entry_ID 50127 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name . _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '3D HNCO' . . . 50127 1 2 '3D HNCA' . . . 50127 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 2 $software_2 . . 50127 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 19 19 GLY H H 1 8.1868 . . . . . . . . 58 G HN . 50127 1 2 . 1 . 1 19 19 GLY N N 15 110.396 . . . . . . . . 58 G N . 50127 1 3 . 1 . 1 20 20 LEU H H 1 8.037 . . . . . . . . 59 L HN . 50127 1 4 . 1 . 1 20 20 LEU N N 15 121.613 . . . . . . . . 59 L N . 50127 1 5 . 1 . 1 22 22 ARG C C 13 176.257 . . . . . . . . 61 R C . 50127 1 6 . 1 . 1 22 22 ARG CA C 13 55.986 . . . . . . . . 61 R CA . 50127 1 7 . 1 . 1 23 23 GLY H H 1 8.314 . . . . . . . . 62 G HN . 50127 1 8 . 1 . 1 23 23 GLY CA C 13 44.614 . . . . . . . . 62 G CA . 50127 1 9 . 1 . 1 23 23 GLY N N 15 110.663 . . . . . . . . 62 G N . 50127 1 10 . 1 . 1 24 24 PRO C C 13 177.298 . . . . . . . . 63 P C . 50127 1 11 . 1 . 1 24 24 PRO CA C 13 63.371 . . . . . . . . 63 P CA . 50127 1 12 . 1 . 1 25 25 SER H H 1 8.497 . . . . . . . . 64 S HN . 50127 1 13 . 1 . 1 25 25 SER C C 13 175.054 . . . . . . . . 64 S C . 50127 1 14 . 1 . 1 25 25 SER CA C 13 58.486 . . . . . . . . 64 S CA . 50127 1 15 . 1 . 1 25 25 SER N N 15 116.419 . . . . . . . . 64 S N . 50127 1 16 . 1 . 1 26 26 THR H H 1 8.205 . . . . . . . . 65 T HN . 50127 1 17 . 1 . 1 26 26 THR C C 13 174.582 . . . . . . . . 65 T C . 50127 1 18 . 1 . 1 26 26 THR CB C 13 69.560 . . . . . . . . 65 T CB . 50127 1 19 . 1 . 1 26 26 THR N N 15 116.006 . . . . . . . . 65 T N . 50127 1 20 . 1 . 1 27 27 GLU H H 1 8.358 . . . . . . . . 66 E HN . 50127 1 21 . 1 . 1 27 27 GLU C C 13 176.777 . . . . . . . . 66 E C . 50127 1 22 . 1 . 1 27 27 GLU CB C 13 30.264 . . . . . . . . 66 E CB . 50127 1 23 . 1 . 1 27 27 GLU N N 15 123.424 . . . . . . . . 66 E N . 50127 1 24 . 1 . 1 28 28 GLY H H 1 8.408 . . . . . . . . 67 G HN . 50127 1 25 . 1 . 1 28 28 GLY C C 13 173.782 . . . . . . . . 67 G C . 50127 1 26 . 1 . 1 28 28 GLY CA C 13 45.309 . . . . . . . . 67 G CA . 50127 1 27 . 1 . 1 28 28 GLY N N 15 110.525 . . . . . . . . 67 G N . 50127 1 28 . 1 . 1 29 29 VAL H H 1 7.919 . . . . . . . . 68 V HN . 50127 1 29 . 1 . 1 29 29 VAL CA C 13 59.899 . . . . . . . . 68 V CA . 50127 1 30 . 1 . 1 29 29 VAL N N 15 120.649 . . . . . . . . 68 V N . 50127 1 31 . 1 . 1 30 30 PRO C C 13 177.295 . . . . . . . . 69 P C . 50127 1 32 . 1 . 1 30 30 PRO CA C 13 63.434 . . . . . . . . 69 P CA . 50127 1 33 . 1 . 1 30 30 PRO CB C 13 32.135 . . . . . . . . 69 P CB . 50127 1 34 . 1 . 1 31 31 GLU H H 1 8.681 . . . . . . . . 70 E HN . 50127 1 35 . 1 . 1 31 31 GLU C C 13 177.052 . . . . . . . . 70 E C . 50127 1 36 . 1 . 1 31 31 GLU CA C 13 57.903 . . . . . . . . 70 E CA . 50127 1 37 . 1 . 1 31 31 GLU CB C 13 29.956 . . . . . . . . 70 E CB . 50127 1 38 . 1 . 1 31 31 GLU N N 15 122.001 . . . . . . . . 70 E N . 50127 1 39 . 1 . 1 32 32 SER H H 1 8.255 . . . . . . . . 71 S HN . 50127 1 40 . 1 . 1 32 32 SER C C 13 174.791 . . . . . . . . 71 S C . 50127 1 41 . 1 . 1 32 32 SER CA C 13 58.899 . . . . . . . . 71 S CA . 50127 1 42 . 1 . 1 32 32 SER CB C 13 63.574 . . . . . . . . 71 S CB . 50127 1 43 . 1 . 1 32 32 SER N N 15 115.412 . . . . . . . . 71 S N . 50127 1 44 . 1 . 1 33 33 MET H H 1 8.201 . . . . . . . . 72 M HN . 50127 1 45 . 1 . 1 33 33 MET C C 13 176.136 . . . . . . . . 72 M C . 50127 1 46 . 1 . 1 33 33 MET CA C 13 55.667 . . . . . . . . 72 M CA . 50127 1 47 . 1 . 1 33 33 MET N N 15 122.073 . . . . . . . . 72 M N . 50127 1 48 . 1 . 1 34 34 ARG H H 1 8.074 . . . . . . . . 73 R HN . 50127 1 49 . 1 . 1 34 34 ARG C C 13 176.509 . . . . . . . . 73 R C . 50127 1 50 . 1 . 1 34 34 ARG CA C 13 56.775 . . . . . . . . 73 R CA . 50127 1 51 . 1 . 1 34 34 ARG CB C 13 30.810 . . . . . . . . 73 R CB . 50127 1 52 . 1 . 1 34 34 ARG N N 15 122.069 . . . . . . . . 73 R N . 50127 1 53 . 1 . 1 35 35 GLU H H 1 8.473 . . . . . . . . 74 E HN . 50127 1 54 . 1 . 1 35 35 GLU C C 13 176.680 . . . . . . . . 74 E C . 50127 1 55 . 1 . 1 35 35 GLU CA C 13 57.079 . . . . . . . . 74 E CA . 50127 1 56 . 1 . 1 35 35 GLU CB C 13 30.016 . . . . . . . . 74 E CB . 50127 1 57 . 1 . 1 35 35 GLU N N 15 122.190 . . . . . . . . 74 E N . 50127 1 58 . 1 . 1 36 36 GLU H H 1 8.436 . . . . . . . . 75 E HN . 50127 1 59 . 1 . 1 36 36 GLU C C 13 176.384 . . . . . . . . 75 E C . 50127 1 60 . 1 . 1 36 36 GLU CA C 13 57.065 . . . . . . . . 75 E CA . 50127 1 61 . 1 . 1 36 36 GLU CB C 13 29.991 . . . . . . . . 75 E CB . 50127 1 62 . 1 . 1 36 36 GLU N N 15 121.541 . . . . . . . . 75 E N . 50127 1 63 . 1 . 1 37 37 TYR H H 1 8.105 . . . . . . . . 76 Y HN . 50127 1 64 . 1 . 1 37 37 TYR C C 13 175.884 . . . . . . . . 76 Y C . 50127 1 65 . 1 . 1 37 37 TYR CA C 13 58.115 . . . . . . . . 76 Y CA . 50127 1 66 . 1 . 1 37 37 TYR CB C 13 38.661 . . . . . . . . 76 Y CB . 50127 1 67 . 1 . 1 37 37 TYR N N 15 121.094 . . . . . . . . 76 Y N . 50127 1 68 . 1 . 1 38 38 GLN H H 1 8.180 . . . . . . . . 77 Q HN . 50127 1 69 . 1 . 1 38 38 GLN C C 13 175.837 . . . . . . . . 77 Q C . 50127 1 70 . 1 . 1 38 38 GLN CA C 13 56.064 . . . . . . . . 77 Q CA . 50127 1 71 . 1 . 1 38 38 GLN CB C 13 29.364 . . . . . . . . 77 Q CB . 50127 1 72 . 1 . 1 38 38 GLN N N 15 122.252 . . . . . . . . 77 Q N . 50127 1 73 . 1 . 1 39 39 GLN H H 1 8.294 . . . . . . . . 78 Q HN . 50127 1 74 . 1 . 1 39 39 GLN C C 13 176.227 . . . . . . . . 78 Q C . 50127 1 75 . 1 . 1 39 39 GLN CA C 13 56.297 . . . . . . . . 78 Q CA . 50127 1 76 . 1 . 1 39 39 GLN CB C 13 29.519 . . . . . . . . 78 Q CB . 50127 1 77 . 1 . 1 39 39 GLN N N 15 121.702 . . . . . . . . 78 Q N . 50127 1 78 . 1 . 1 40 40 GLU H H 1 8.460 . . . . . . . . 79 E HN . 50127 1 79 . 1 . 1 40 40 GLU C C 13 176.590 . . . . . . . . 79 E C . 50127 1 80 . 1 . 1 40 40 GLU CA C 13 56.900 . . . . . . . . 79 E CA . 50127 1 81 . 1 . 1 40 40 GLU CB C 13 30.120 . . . . . . . . 79 E CB . 50127 1 82 . 1 . 1 40 40 GLU N N 15 122.297 . . . . . . . . 79 E N . 50127 1 83 . 1 . 1 41 41 GLN H H 1 8.376 . . . . . . . . 80 Q HN . 50127 1 84 . 1 . 1 41 41 GLN C C 13 176.054 . . . . . . . . 80 Q C . 50127 1 85 . 1 . 1 41 41 GLN CA C 13 56.071 . . . . . . . . 80 Q CA . 50127 1 86 . 1 . 1 41 41 GLN CB C 13 29.504 . . . . . . . . 80 Q CB . 50127 1 87 . 1 . 1 41 41 GLN N N 15 121.555 . . . . . . . . 80 Q N . 50127 1 88 . 1 . 1 42 42 GLN H H 1 8.415 . . . . . . . . 81 Q HN . 50127 1 89 . 1 . 1 42 42 GLN C C 13 175.995 . . . . . . . . 81 Q C . 50127 1 90 . 1 . 1 42 42 GLN CA C 13 56.104 . . . . . . . . 81 Q CA . 50127 1 91 . 1 . 1 42 42 GLN CB C 13 29.569 . . . . . . . . 81 Q CB . 50127 1 92 . 1 . 1 42 42 GLN N N 15 122.057 . . . . . . . . 81 Q N . 50127 1 93 . 1 . 1 43 43 SER H H 1 8.353 . . . . . . . . 82 S HN . 50127 1 94 . 1 . 1 43 43 SER C C 13 174.127 . . . . . . . . 82 S C . 50127 1 95 . 1 . 1 43 43 SER CA C 13 58.350 . . . . . . . . 82 S CA . 50127 1 96 . 1 . 1 43 43 SER CB C 13 63.952 . . . . . . . . 82 S CB . 50127 1 97 . 1 . 1 43 43 SER N N 15 117.554 . . . . . . . . 82 S N . 50127 1 98 . 1 . 1 44 44 ALA H H 1 8.335 . . . . . . . . 83 A HN . 50127 1 99 . 1 . 1 44 44 ALA C C 13 177.490 . . . . . . . . 83 A C . 50127 1 100 . 1 . 1 44 44 ALA CA C 13 52.588 . . . . . . . . 83 A CA . 50127 1 101 . 1 . 1 44 44 ALA CB C 13 19.395 . . . . . . . . 83 A CB . 50127 1 102 . 1 . 1 44 44 ALA N N 15 126.702 . . . . . . . . 83 A N . 50127 1 103 . 1 . 1 45 45 VAL H H 1 8.006 . . . . . . . . 84 V HN . 50127 1 104 . 1 . 1 45 45 VAL C C 13 175.720 . . . . . . . . 84 V C . 50127 1 105 . 1 . 1 45 45 VAL CA C 13 62.142 . . . . . . . . 84 V CA . 50127 1 106 . 1 . 1 45 45 VAL CB C 13 32.962 . . . . . . . . 84 V CB . 50127 1 107 . 1 . 1 45 45 VAL N N 15 118.672 . . . . . . . . 84 V N . 50127 1 108 . 1 . 1 46 46 ASP H H 1 8.359 . . . . . . . . 85 D HN . 50127 1 109 . 1 . 1 46 46 ASP C C 13 176.049 . . . . . . . . 85 D C . 50127 1 110 . 1 . 1 46 46 ASP CA C 13 54.254 . . . . . . . . 85 D CA . 50127 1 111 . 1 . 1 46 46 ASP CB C 13 41.086 . . . . . . . . 85 D CB . 50127 1 112 . 1 . 1 46 46 ASP N N 15 124.243 . . . . . . . . 85 D N . 50127 1 113 . 1 . 1 47 47 VAL H H 1 8.038 . . . . . . . . 86 V HN . 50127 1 114 . 1 . 1 47 47 VAL C C 13 175.786 . . . . . . . . 86 V C . 50127 1 115 . 1 . 1 47 47 VAL CA C 13 62.235 . . . . . . . . 86 V CA . 50127 1 116 . 1 . 1 47 47 VAL CB C 13 32.984 . . . . . . . . 86 V CB . 50127 1 117 . 1 . 1 47 47 VAL N N 15 120.054 . . . . . . . . 86 V N . 50127 1 118 . 1 . 1 48 48 ASP H H 1 8.391 . . . . . . . . 87 D HN . 50127 1 119 . 1 . 1 48 48 ASP C C 13 175.962 . . . . . . . . 87 D C . 50127 1 120 . 1 . 1 48 48 ASP CA C 13 54.282 . . . . . . . . 87 D CA . 50127 1 121 . 1 . 1 48 48 ASP CB C 13 41.229 . . . . . . . . 87 D CB . 50127 1 122 . 1 . 1 48 48 ASP N N 15 124.056 . . . . . . . . 87 D N . 50127 1 123 . 1 . 1 49 49 ASP H H 1 8.268 . . . . . . . . 88 D HN . 50127 1 124 . 1 . 1 49 49 ASP C C 13 177.047 . . . . . . . . 88 D C . 50127 1 125 . 1 . 1 49 49 ASP CA C 13 54.455 . . . . . . . . 88 D CA . 50127 1 126 . 1 . 1 49 49 ASP CB C 13 40.972 . . . . . . . . 88 D CB . 50127 1 127 . 1 . 1 49 49 ASP N N 15 122.101 . . . . . . . . 88 D N . 50127 1 128 . 1 . 1 50 50 GLY H H 1 8.451 . . . . . . . . 89 G HN . 50127 1 129 . 1 . 1 50 50 GLY C C 13 174.441 . . . . . . . . 89 G C . 50127 1 130 . 1 . 1 50 50 GLY CA C 13 45.796 . . . . . . . . 89 G CA . 50127 1 131 . 1 . 1 50 50 GLY N N 15 109.293 . . . . . . . . 89 G N . 50127 1 132 . 1 . 1 51 51 HIS H H 1 8.195 . . . . . . . . 90 H HN . 50127 1 133 . 1 . 1 51 51 HIS C C 13 174.091 . . . . . . . . 90 H C . 50127 1 134 . 1 . 1 51 51 HIS CA C 13 55.683 . . . . . . . . 90 H CA . 50127 1 135 . 1 . 1 51 51 HIS CB C 13 28.629 . . . . . . . . 90 H CB . 50127 1 136 . 1 . 1 51 51 HIS N N 15 118.387 . . . . . . . . 90 H N . 50127 1 137 . 1 . 1 52 52 PHE H H 1 8.305 . . . . . . . . 91 F HN . 50127 1 138 . 1 . 1 52 52 PHE C C 13 175.395 . . . . . . . . 91 F C . 50127 1 139 . 1 . 1 52 52 PHE CA C 13 57.871 . . . . . . . . 91 F CA . 50127 1 140 . 1 . 1 52 52 PHE CB C 13 39.577 . . . . . . . . 91 F CB . 50127 1 141 . 1 . 1 52 52 PHE N N 15 121.855 . . . . . . . . 91 F N . 50127 1 142 . 1 . 1 53 53 VAL H H 1 7.996 . . . . . . . . 92 V HN . 50127 1 143 . 1 . 1 53 53 VAL C C 13 175.094 . . . . . . . . 92 V C . 50127 1 144 . 1 . 1 53 53 VAL CA C 13 62.030 . . . . . . . . 92 V CA . 50127 1 145 . 1 . 1 53 53 VAL CB C 13 33.177 . . . . . . . . 92 V CB . 50127 1 146 . 1 . 1 53 53 VAL N N 15 122.474 . . . . . . . . 92 V N . 50127 1 147 . 1 . 1 54 54 ASN H H 1 8.435 . . . . . . . . 93 N HN . 50127 1 148 . 1 . 1 54 54 ASN C C 13 174.919 . . . . . . . . 93 N C . 50127 1 149 . 1 . 1 54 54 ASN CA C 13 53.160 . . . . . . . . 93 N CA . 50127 1 150 . 1 . 1 54 54 ASN CB C 13 39.044 . . . . . . . . 93 N CB . 50127 1 151 . 1 . 1 54 54 ASN N N 15 123.307 . . . . . . . . 93 N N . 50127 1 152 . 1 . 1 55 55 ILE H H 1 8.092 . . . . . . . . 94 I HN . 50127 1 153 . 1 . 1 55 55 ILE C C 13 175.854 . . . . . . . . 94 I C . 50127 1 154 . 1 . 1 55 55 ILE CA C 13 61.293 . . . . . . . . 94 I CA . 50127 1 155 . 1 . 1 55 55 ILE CB C 13 38.944 . . . . . . . . 94 I CB . 50127 1 156 . 1 . 1 55 55 ILE N N 15 122.016 . . . . . . . . 94 I N . 50127 1 157 . 1 . 1 56 56 GLU H H 1 8.412 . . . . . . . . 95 E HN . 50127 1 158 . 1 . 1 56 56 GLU C C 13 176.013 . . . . . . . . 95 E C . 50127 1 159 . 1 . 1 56 56 GLU CA C 13 56.375 . . . . . . . . 95 E CA . 50127 1 160 . 1 . 1 56 56 GLU CB C 13 30.012 . . . . . . . . 95 E CB . 50127 1 161 . 1 . 1 56 56 GLU N N 15 124.919 . . . . . . . . 95 E N . 50127 1 162 . 1 . 1 57 57 LEU H H 1 8.203 . . . . . . . . 96 L HN . 50127 1 163 . 1 . 1 57 57 LEU C C 13 176.325 . . . . . . . . 96 L C . 50127 1 164 . 1 . 1 57 57 LEU CA C 13 55.169 . . . . . . . . 96 L CA . 50127 1 165 . 1 . 1 57 57 LEU CB C 13 42.607 . . . . . . . . 96 L CB . 50127 1 166 . 1 . 1 57 57 LEU N N 15 124.283 . . . . . . . . 96 L N . 50127 1 167 . 1 . 1 58 58 GLU H H 1 7.881 . . . . . . . . 97 E HN . 50127 1 168 . 1 . 1 58 58 GLU CA C 13 57.845 . . . . . . . . 97 E CA . 50127 1 169 . 1 . 1 58 58 GLU N N 15 126.733 . . . . . . . . 97 E N . 50127 1 stop_ save_