data_50234 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 50234 _Entry.Title ; 15N-spin relaxation data for TRBP2-dsRBD1 dynamics in presence of D10-RNA ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2020-04-14 _Entry.Accession_date 2020-04-14 _Entry.Last_release_date 2020-04-22 _Entry.Original_release_date 2020-04-22 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details '15N-spin relaxation data for TRBP2-dsRBD1 dynamics in presence of D10-RNA' _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Harshad Paithankar . . . 0000-0003-0693-9417 50234 2 Jeetender Chugh . . . . 50234 stop_ loop_ _Entry_src.ID _Entry_src.Project_name _Entry_src.Organization_full_name _Entry_src.Organization_initials _Entry_src.Entry_ID 1 . 'Indian Institute of Science, Education, and Research' . 50234 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID heteronucl_NOEs 1 50234 heteronucl_T1_relaxation 1 50234 heteronucl_T2_relaxation 1 50234 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID 'T1 relaxation values' 58 50234 'T2 relaxation values' 58 50234 'heteronuclear NOE values' 58 50234 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2022-02-16 . original BMRB . 50234 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 27262 'Chemical shift assignment for the protein TRBP2-dsRBD1' 50234 BMRB 50233 'Model-free analysis of 15N spin-relaxation data for TRBP2-dsRBD1' 50234 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 50234 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID 35134335 _Citation.DOI 10.1016/j.bpj.2022.02.005 _Citation.Full_citation . _Citation.Title ; Relay of intrinsic microsecond timescale conformational dynamics observed in an RNA-binding domain of TRBP upon enthalpy-driven binding of dsRNA ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Biophys. J.' _Citation.Journal_name_full . _Citation.Journal_volume . _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first . _Citation.Page_last . _Citation.Year 2022 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Harshad Paithankar H. . . . 50234 1 2 'Guneet Singh' Tarang G. S. . . 50234 1 3 Firdousi Parvez F. . . . 50234 1 4 Aniket Marathe A. . . . 50234 1 5 Manali Joshi M. . . . 50234 1 6 Jeetender Chugh J. . . . 50234 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 50234 _Assembly.ID 1 _Assembly.Name 'TRBP2-dsRBD1:D10-RNA complex' _Assembly.BMRB_code . _Assembly.Number_of_components 3 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states yes _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange yes _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details 'D10-RNA is an RNA duplex with 10 base-pairs.' _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 TRBP2-dsRBD1 1 $entity_1 . . yes native no no . . . 50234 1 2 'D10-RNA, chain 1' 2 $entity_2 . . no native no no . . . 50234 1 3 'D10-RNA, chain 2' 3 $entity_3 . . no native no no . . . 50234 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 50234 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; GGAMSEEEQGSGTTTGCGLP SIEQMLAANPGKTPISLLQE YGTRIGKTPVYDLLKAEGQA HQPNFTFRVTVGDTSCTGQG PSKKAAKHKAAEVALKHLKG GSMLEPAL ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details 'N-terminal residues GGA are non-native to the protein and are left after the cleavage of the fusion tag using protease.' _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 108 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state unknown _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_biological_function.Biological_function _Entity_biological_function.Entry_ID _Entity_biological_function.Entity_ID 'dsRNA-binding protein' 50234 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . GLY . 50234 1 2 . GLY . 50234 1 3 . ALA . 50234 1 4 . MET . 50234 1 5 . SER . 50234 1 6 . GLU . 50234 1 7 . GLU . 50234 1 8 . GLU . 50234 1 9 . GLN . 50234 1 10 . GLY . 50234 1 11 . SER . 50234 1 12 . GLY . 50234 1 13 . THR . 50234 1 14 . THR . 50234 1 15 . THR . 50234 1 16 . GLY . 50234 1 17 . CYS . 50234 1 18 . GLY . 50234 1 19 . LEU . 50234 1 20 . PRO . 50234 1 21 . SER . 50234 1 22 . ILE . 50234 1 23 . GLU . 50234 1 24 . GLN . 50234 1 25 . MET . 50234 1 26 . LEU . 50234 1 27 . ALA . 50234 1 28 . ALA . 50234 1 29 . ASN . 50234 1 30 . PRO . 50234 1 31 . GLY . 50234 1 32 . LYS . 50234 1 33 . THR . 50234 1 34 . PRO . 50234 1 35 . ILE . 50234 1 36 . SER . 50234 1 37 . LEU . 50234 1 38 . LEU . 50234 1 39 . GLN . 50234 1 40 . GLU . 50234 1 41 . TYR . 50234 1 42 . GLY . 50234 1 43 . THR . 50234 1 44 . ARG . 50234 1 45 . ILE . 50234 1 46 . GLY . 50234 1 47 . LYS . 50234 1 48 . THR . 50234 1 49 . PRO . 50234 1 50 . VAL . 50234 1 51 . TYR . 50234 1 52 . ASP . 50234 1 53 . LEU . 50234 1 54 . LEU . 50234 1 55 . LYS . 50234 1 56 . ALA . 50234 1 57 . GLU . 50234 1 58 . GLY . 50234 1 59 . GLN . 50234 1 60 . ALA . 50234 1 61 . HIS . 50234 1 62 . GLN . 50234 1 63 . PRO . 50234 1 64 . ASN . 50234 1 65 . PHE . 50234 1 66 . THR . 50234 1 67 . PHE . 50234 1 68 . ARG . 50234 1 69 . VAL . 50234 1 70 . THR . 50234 1 71 . VAL . 50234 1 72 . GLY . 50234 1 73 . ASP . 50234 1 74 . THR . 50234 1 75 . SER . 50234 1 76 . CYS . 50234 1 77 . THR . 50234 1 78 . GLY . 50234 1 79 . GLN . 50234 1 80 . GLY . 50234 1 81 . PRO . 50234 1 82 . SER . 50234 1 83 . LYS . 50234 1 84 . LYS . 50234 1 85 . ALA . 50234 1 86 . ALA . 50234 1 87 . LYS . 50234 1 88 . HIS . 50234 1 89 . LYS . 50234 1 90 . ALA . 50234 1 91 . ALA . 50234 1 92 . GLU . 50234 1 93 . VAL . 50234 1 94 . ALA . 50234 1 95 . LEU . 50234 1 96 . LYS . 50234 1 97 . HIS . 50234 1 98 . LEU . 50234 1 99 . LYS . 50234 1 100 . GLY . 50234 1 101 . GLY . 50234 1 102 . SER . 50234 1 103 . MET . 50234 1 104 . LEU . 50234 1 105 . GLU . 50234 1 106 . PRO . 50234 1 107 . ALA . 50234 1 108 . LEU . 50234 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . GLY 1 1 50234 1 . GLY 2 2 50234 1 . ALA 3 3 50234 1 . MET 4 4 50234 1 . SER 5 5 50234 1 . GLU 6 6 50234 1 . GLU 7 7 50234 1 . GLU 8 8 50234 1 . GLN 9 9 50234 1 . GLY 10 10 50234 1 . SER 11 11 50234 1 . GLY 12 12 50234 1 . THR 13 13 50234 1 . THR 14 14 50234 1 . THR 15 15 50234 1 . GLY 16 16 50234 1 . CYS 17 17 50234 1 . GLY 18 18 50234 1 . LEU 19 19 50234 1 . PRO 20 20 50234 1 . SER 21 21 50234 1 . ILE 22 22 50234 1 . GLU 23 23 50234 1 . GLN 24 24 50234 1 . MET 25 25 50234 1 . LEU 26 26 50234 1 . ALA 27 27 50234 1 . ALA 28 28 50234 1 . ASN 29 29 50234 1 . PRO 30 30 50234 1 . GLY 31 31 50234 1 . LYS 32 32 50234 1 . THR 33 33 50234 1 . PRO 34 34 50234 1 . ILE 35 35 50234 1 . SER 36 36 50234 1 . LEU 37 37 50234 1 . LEU 38 38 50234 1 . GLN 39 39 50234 1 . GLU 40 40 50234 1 . TYR 41 41 50234 1 . GLY 42 42 50234 1 . THR 43 43 50234 1 . ARG 44 44 50234 1 . ILE 45 45 50234 1 . GLY 46 46 50234 1 . LYS 47 47 50234 1 . THR 48 48 50234 1 . PRO 49 49 50234 1 . VAL 50 50 50234 1 . TYR 51 51 50234 1 . ASP 52 52 50234 1 . LEU 53 53 50234 1 . LEU 54 54 50234 1 . LYS 55 55 50234 1 . ALA 56 56 50234 1 . GLU 57 57 50234 1 . GLY 58 58 50234 1 . GLN 59 59 50234 1 . ALA 60 60 50234 1 . HIS 61 61 50234 1 . GLN 62 62 50234 1 . PRO 63 63 50234 1 . ASN 64 64 50234 1 . PHE 65 65 50234 1 . THR 66 66 50234 1 . PHE 67 67 50234 1 . ARG 68 68 50234 1 . VAL 69 69 50234 1 . THR 70 70 50234 1 . VAL 71 71 50234 1 . GLY 72 72 50234 1 . ASP 73 73 50234 1 . THR 74 74 50234 1 . SER 75 75 50234 1 . CYS 76 76 50234 1 . THR 77 77 50234 1 . GLY 78 78 50234 1 . GLN 79 79 50234 1 . GLY 80 80 50234 1 . PRO 81 81 50234 1 . SER 82 82 50234 1 . LYS 83 83 50234 1 . LYS 84 84 50234 1 . ALA 85 85 50234 1 . ALA 86 86 50234 1 . LYS 87 87 50234 1 . HIS 88 88 50234 1 . LYS 89 89 50234 1 . ALA 90 90 50234 1 . ALA 91 91 50234 1 . GLU 92 92 50234 1 . VAL 93 93 50234 1 . ALA 94 94 50234 1 . LEU 95 95 50234 1 . LYS 96 96 50234 1 . HIS 97 97 50234 1 . LEU 98 98 50234 1 . LYS 99 99 50234 1 . GLY 100 100 50234 1 . GLY 101 101 50234 1 . SER 102 102 50234 1 . MET 103 103 50234 1 . LEU 104 104 50234 1 . GLU 105 105 50234 1 . PRO 106 106 50234 1 . ALA 107 107 50234 1 . LEU 108 108 50234 1 stop_ save_ save_entity_2 _Entity.Sf_category entity _Entity.Sf_framecode entity_2 _Entity.Entry_ID 50234 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name entity_2 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polyribonucleotide _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; UUAUAAAUGC ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details 5'-UUAUAAAUGC-3' _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 10 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID 2 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details ; The two strands with the sequence mentioned above are annealed by heating at 95 C for 10 min followed by cooling on ice for 10 min before titrating it with protein. ; _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_biological_function.Biological_function _Entity_biological_function.Entry_ID _Entity_biological_function.Entity_ID 'dsRNA substrate for TRBP2-dsRBD1' 50234 2 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . U . 50234 2 2 . U . 50234 2 3 . A . 50234 2 4 . U . 50234 2 5 . A . 50234 2 6 . A . 50234 2 7 . A . 50234 2 8 . U . 50234 2 9 . G . 50234 2 10 . C . 50234 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . U 1 1 50234 2 . U 2 2 50234 2 . A 3 3 50234 2 . U 4 4 50234 2 . A 5 5 50234 2 . A 6 6 50234 2 . A 7 7 50234 2 . U 8 8 50234 2 . G 9 9 50234 2 . C 10 10 50234 2 stop_ save_ save_entity_3 _Entity.Sf_category entity _Entity.Sf_framecode entity_3 _Entity.Entry_ID 50234 _Entity.ID 3 _Entity.BMRB_code . _Entity.Name entity_3 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polyribonucleotide _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; GCAUUUAUGA ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details 5'-GCAUUUAUGA-3' _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 10 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID 3 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details ; The two strands with the sequence mentioned above are annealed by heating at 95 C for 10 min followed by cooling on ice for 10 min before titrating it with protein. ; _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_biological_function.Biological_function _Entity_biological_function.Entry_ID _Entity_biological_function.Entity_ID 'dsRNA substrate for TRBP2-dsRBD1' 50234 3 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . G . 50234 3 2 . C . 50234 3 3 . A . 50234 3 4 . U . 50234 3 5 . U . 50234 3 6 . U . 50234 3 7 . A . 50234 3 8 . U . 50234 3 9 . G . 50234 3 10 . A . 50234 3 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . G 1 1 50234 3 . C 2 2 50234 3 . A 3 3 50234 3 . U 4 4 50234 3 . U 5 5 50234 3 . U 6 6 50234 3 . A 7 7 50234 3 . U 8 8 50234 3 . G 9 9 50234 3 . A 10 10 50234 3 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 50234 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 50234 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 50234 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli BL21(DE3) . . plasmid . . pSV272 . . . 50234 1 2 2 $entity_2 . 'obtained from a vendor' . . . . . . . . . plasmid . . . . . . 50234 1 3 3 $entity_3 . 'obtained from a vendor' . . . . . . . . . plasmid . . . . . . 50234 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 50234 _Sample.ID 1 _Sample.Name TRBP2-dsRBD1 _Sample.Type solution _Sample.Sub_type . _Sample.Details ; Protein in 0.5 equivalents of D10-RNA Buffer Conditions 10mM Sodium phosphate, 10mM NaCl, 1mM EDTA, 1mM DTT pH 6.4 ; _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 TRBP2-dsRBD1 '[U-100% 15N]' . . 1 $entity_1 . . 200 . . uM . . . . 50234 1 2 'D10-RNA, chain 1' 'natural abundance' . . 2 $entity_2 . . 100 . . uM . . . . 50234 1 3 'D10-RNA, chain 2' 'natural abundance' . . 3 $entity_3 . . 100 . . uM . . . . 50234 1 4 D2O [U-2H] . . . . . . 10 . . % . . . . 50234 1 5 'sodium phosphate' 'natural abundance' . . . . . . 10 . . mM . . . . 50234 1 6 'sodium chloride' 'natural abundance' . . . . . . 10 . . mM . . . . 50234 1 7 EDTA 'natural abundance' . . . . . . 1 . . mM . . . . 50234 1 8 DTT 'natural abundance' . . . . . . 1 . . mM . . . . 50234 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 50234 _Sample_condition_list.ID 1 _Sample_condition_list.Name Sample_conditions_1 _Sample_condition_list.Details ; Buffer 10mM Sodium phosphate, 10mM NaCl, 1mM EDTA, 1mM DTT pH 6.4 298 K ; loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 100 . mM 50234 1 pH 6.4 . pH 50234 1 pressure 1 . atm 50234 1 temperature 298 . K 50234 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 50234 _Software.ID 1 _Software.Type . _Software.Name TOPSPIN _Software.Version 3.5 _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID collection . 50234 1 stop_ save_ save_software_2 _Software.Sf_category software _Software.Sf_framecode software_2 _Software.Entry_ID 50234 _Software.ID 2 _Software.Type . _Software.Name NMRPipe _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID processing . 50234 2 stop_ save_ save_software_3 _Software.Sf_category software _Software.Sf_framecode software_3 _Software.Entry_ID 50234 _Software.ID 3 _Software.Type . _Software.Name NMRFAM-SPARKY _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'peak picking' . 50234 3 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 50234 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name '600MHz NMR spectrometer' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model Avance _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 50234 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 'T1/R1 relaxation' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50234 1 2 'T2/R2 relaxation' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50234 1 3 '1H-15N heteronoe' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50234 1 stop_ save_ ############################## # Heteronuclear NOE values # ############################## save_heteronucl_NOEs_1 _Heteronucl_NOE_list.Sf_category heteronucl_NOEs _Heteronucl_NOE_list.Sf_framecode heteronucl_NOEs_1 _Heteronucl_NOE_list.Entry_ID 50234 _Heteronucl_NOE_list.ID 1 _Heteronucl_NOE_list.Name NOE-600 _Heteronucl_NOE_list.Sample_condition_list_ID 1 _Heteronucl_NOE_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_NOE_list.Spectrometer_frequency_1H 600.4021885 _Heteronucl_NOE_list.Heteronuclear_NOE_val_type 'peak height' _Heteronucl_NOE_list.NOE_ref_val 0 _Heteronucl_NOE_list.NOE_ref_description . _Heteronucl_NOE_list.Details . _Heteronucl_NOE_list.Text_data_format . _Heteronucl_NOE_list.Text_data . loop_ _Heteronucl_NOE_experiment.Experiment_ID _Heteronucl_NOE_experiment.Experiment_name _Heteronucl_NOE_experiment.Sample_ID _Heteronucl_NOE_experiment.Sample_label _Heteronucl_NOE_experiment.Sample_state _Heteronucl_NOE_experiment.Entry_ID _Heteronucl_NOE_experiment.Heteronucl_NOE_list_ID 3 '1H-15N heteronoe' . . . 50234 1 stop_ loop_ _Heteronucl_NOE_software.Software_ID _Heteronucl_NOE_software.Software_label _Heteronucl_NOE_software.Method_ID _Heteronucl_NOE_software.Method_label _Heteronucl_NOE_software.Entry_ID _Heteronucl_NOE_software.Heteronucl_NOE_list_ID 1 $software_1 . . 50234 1 2 $software_2 . . 50234 1 3 $software_3 . . 50234 1 stop_ loop_ _Heteronucl_NOE.ID _Heteronucl_NOE.Assembly_atom_ID_1 _Heteronucl_NOE.Entity_assembly_ID_1 _Heteronucl_NOE.Entity_ID_1 _Heteronucl_NOE.Comp_index_ID_1 _Heteronucl_NOE.Seq_ID_1 _Heteronucl_NOE.Comp_ID_1 _Heteronucl_NOE.Atom_ID_1 _Heteronucl_NOE.Atom_type_1 _Heteronucl_NOE.Atom_isotope_number_1 _Heteronucl_NOE.Assembly_atom_ID_2 _Heteronucl_NOE.Entity_assembly_ID_2 _Heteronucl_NOE.Entity_ID_2 _Heteronucl_NOE.Comp_index_ID_2 _Heteronucl_NOE.Seq_ID_2 _Heteronucl_NOE.Comp_ID_2 _Heteronucl_NOE.Atom_ID_2 _Heteronucl_NOE.Atom_type_2 _Heteronucl_NOE.Atom_isotope_number_2 _Heteronucl_NOE.Val _Heteronucl_NOE.Val_err _Heteronucl_NOE.Resonance_ID_1 _Heteronucl_NOE.Resonance_ID_2 _Heteronucl_NOE.Auth_entity_assembly_ID_1 _Heteronucl_NOE.Auth_seq_ID_1 _Heteronucl_NOE.Auth_comp_ID_1 _Heteronucl_NOE.Auth_atom_ID_1 _Heteronucl_NOE.Auth_entity_assembly_ID_2 _Heteronucl_NOE.Auth_seq_ID_2 _Heteronucl_NOE.Auth_comp_ID_2 _Heteronucl_NOE.Auth_atom_ID_2 _Heteronucl_NOE.Entry_ID _Heteronucl_NOE.Heteronucl_NOE_list_ID 1 . 1 1 5 5 SER N N 15 . 1 1 5 5 SER H H 1 -0.64948 0.10724 . . . . . . . . . . 50234 1 2 . 1 1 6 6 GLU N N 15 . 1 1 6 6 GLU H H 1 -0.11926 0.10724 . . . . . . . . . . 50234 1 3 . 1 1 8 8 GLU N N 15 . 1 1 8 8 GLU H H 1 -0.06454 0.10724 . . . . . . . . . . 50234 1 4 . 1 1 11 11 SER N N 15 . 1 1 11 11 SER H H 1 -0.07812 0.10724 . . . . . . . . . . 50234 1 5 . 1 1 12 12 GLY N N 15 . 1 1 12 12 GLY H H 1 -0.14523 0.10724 . . . . . . . . . . 50234 1 6 . 1 1 13 13 THR N N 15 . 1 1 13 13 THR H H 1 -0.2865 0.10724 . . . . . . . . . . 50234 1 7 . 1 1 14 14 THR N N 15 . 1 1 14 14 THR H H 1 -0.14249 0.10724 . . . . . . . . . . 50234 1 8 . 1 1 15 15 THR N N 15 . 1 1 15 15 THR H H 1 -0.07796 0.10724 . . . . . . . . . . 50234 1 9 . 1 1 16 16 GLY N N 15 . 1 1 16 16 GLY H H 1 -0.1112 0.10724 . . . . . . . . . . 50234 1 10 . 1 1 19 19 LEU N N 15 . 1 1 19 19 LEU H H 1 0.03271 0.10724 . . . . . . . . . . 50234 1 11 . 1 1 21 21 SER N N 15 . 1 1 21 21 SER H H 1 0.34385 0.10724 . . . . . . . . . . 50234 1 12 . 1 1 27 27 ALA N N 15 . 1 1 27 27 ALA H H 1 0.39246 0.10724 . . . . . . . . . . 50234 1 13 . 1 1 28 28 ALA N N 15 . 1 1 28 28 ALA H H 1 0.52968 0.10724 . . . . . . . . . . 50234 1 14 . 1 1 29 29 ASN N N 15 . 1 1 29 29 ASN H H 1 0.39757 0.10724 . . . . . . . . . . 50234 1 15 . 1 1 31 31 GLY N N 15 . 1 1 31 31 GLY H H 1 0.45342 0.10724 . . . . . . . . . . 50234 1 16 . 1 1 33 33 THR N N 15 . 1 1 33 33 THR H H 1 0.56608 0.10724 . . . . . . . . . . 50234 1 17 . 1 1 36 36 SER N N 15 . 1 1 36 36 SER H H 1 0.75078 0.10724 . . . . . . . . . . 50234 1 18 . 1 1 37 37 LEU N N 15 . 1 1 37 37 LEU H H 1 0.63626 0.10724 . . . . . . . . . . 50234 1 19 . 1 1 39 39 GLN N N 15 . 1 1 39 39 GLN H H 1 0.76312 0.10724 . . . . . . . . . . 50234 1 20 . 1 1 42 42 GLY N N 15 . 1 1 42 42 GLY H H 1 0.82017 0.10724 . . . . . . . . . . 50234 1 21 . 1 1 44 44 ARG N N 15 . 1 1 44 44 ARG H H 1 0.71436 0.10724 . . . . . . . . . . 50234 1 22 . 1 1 45 45 ILE N N 15 . 1 1 45 45 ILE H H 1 0.74263 0.10724 . . . . . . . . . . 50234 1 23 . 1 1 46 46 GLY N N 15 . 1 1 46 46 GLY H H 1 0.71112 0.10724 . . . . . . . . . . 50234 1 24 . 1 1 47 47 LYS N N 15 . 1 1 47 47 LYS H H 1 0.33313 0.10724 . . . . . . . . . . 50234 1 25 . 1 1 51 51 TYR N N 15 . 1 1 51 51 TYR H H 1 0.90597 0.10724 . . . . . . . . . . 50234 1 26 . 1 1 52 52 ASP N N 15 . 1 1 52 52 ASP H H 1 0.7924 0.10724 . . . . . . . . . . 50234 1 27 . 1 1 53 53 LEU N N 15 . 1 1 53 53 LEU H H 1 0.65401 0.10724 . . . . . . . . . . 50234 1 28 . 1 1 54 54 LEU N N 15 . 1 1 54 54 LEU H H 1 0.76219 0.10724 . . . . . . . . . . 50234 1 29 . 1 1 56 56 ALA N N 15 . 1 1 56 56 ALA H H 1 0.50195 0.10724 . . . . . . . . . . 50234 1 30 . 1 1 57 57 GLU N N 15 . 1 1 57 57 GLU H H 1 0.41002 0.10724 . . . . . . . . . . 50234 1 31 . 1 1 59 59 GLN N N 15 . 1 1 59 59 GLN H H 1 0.31922 0.10724 . . . . . . . . . . 50234 1 32 . 1 1 64 64 ASN N N 15 . 1 1 64 64 ASN H H 1 0.54613 0.10724 . . . . . . . . . . 50234 1 33 . 1 1 65 65 PHE N N 15 . 1 1 65 65 PHE H H 1 0.72533 0.10724 . . . . . . . . . . 50234 1 34 . 1 1 66 66 THR N N 15 . 1 1 66 66 THR H H 1 0.74309 0.10724 . . . . . . . . . . 50234 1 35 . 1 1 67 67 PHE N N 15 . 1 1 67 67 PHE H H 1 0.75407 0.10724 . . . . . . . . . . 50234 1 36 . 1 1 68 68 ARG N N 15 . 1 1 68 68 ARG H H 1 1.10429 0.10724 . . . . . . . . . . 50234 1 37 . 1 1 69 69 VAL N N 15 . 1 1 69 69 VAL H H 1 0.83501 0.10724 . . . . . . . . . . 50234 1 38 . 1 1 70 70 THR N N 15 . 1 1 70 70 THR H H 1 0.50558 0.10724 . . . . . . . . . . 50234 1 39 . 1 1 71 71 VAL N N 15 . 1 1 71 71 VAL H H 1 0.87486 0.10724 . . . . . . . . . . 50234 1 40 . 1 1 72 72 GLY N N 15 . 1 1 72 72 GLY H H 1 0.79329 0.10724 . . . . . . . . . . 50234 1 41 . 1 1 73 73 ASP N N 15 . 1 1 73 73 ASP H H 1 0.671 0.10724 . . . . . . . . . . 50234 1 42 . 1 1 74 74 THR N N 15 . 1 1 74 74 THR H H 1 0.65668 0.10724 . . . . . . . . . . 50234 1 43 . 1 1 76 76 CYS N N 15 . 1 1 76 76 CYS H H 1 0.71288 0.10724 . . . . . . . . . . 50234 1 44 . 1 1 78 78 GLY N N 15 . 1 1 78 78 GLY H H 1 0.7181 0.10724 . . . . . . . . . . 50234 1 45 . 1 1 80 80 GLY N N 15 . 1 1 80 80 GLY H H 1 0.83564 0.10724 . . . . . . . . . . 50234 1 46 . 1 1 83 83 LYS N N 15 . 1 1 83 83 LYS H H 1 0.65251 0.10724 . . . . . . . . . . 50234 1 47 . 1 1 85 85 ALA N N 15 . 1 1 85 85 ALA H H 1 0.7342 0.10724 . . . . . . . . . . 50234 1 48 . 1 1 91 91 ALA N N 15 . 1 1 91 91 ALA H H 1 0.50116 0.10724 . . . . . . . . . . 50234 1 49 . 1 1 92 92 GLU N N 15 . 1 1 92 92 GLU H H 1 0.59554 0.10724 . . . . . . . . . . 50234 1 50 . 1 1 93 93 VAL N N 15 . 1 1 93 93 VAL H H 1 -0.4737 0.10724 . . . . . . . . . . 50234 1 51 . 1 1 97 97 HIS N N 15 . 1 1 97 97 HIS H H 1 0.48351 0.10724 . . . . . . . . . . 50234 1 52 . 1 1 98 98 LEU N N 15 . 1 1 98 98 LEU H H 1 0.07196 0.10724 . . . . . . . . . . 50234 1 53 . 1 1 100 100 GLY N N 15 . 1 1 100 100 GLY H H 1 0.49339 0.10724 . . . . . . . . . . 50234 1 54 . 1 1 101 101 GLY N N 15 . 1 1 101 101 GLY H H 1 0.46051 0.10724 . . . . . . . . . . 50234 1 55 . 1 1 102 102 SER N N 15 . 1 1 102 102 SER H H 1 0.40185 0.10724 . . . . . . . . . . 50234 1 56 . 1 1 104 104 LEU N N 15 . 1 1 104 104 LEU H H 1 -0.20548 0.10724 . . . . . . . . . . 50234 1 57 . 1 1 107 107 ALA N N 15 . 1 1 107 107 ALA H H 1 -0.67374 0.10724 . . . . . . . . . . 50234 1 58 . 1 1 108 108 LEU N N 15 . 1 1 108 108 LEU H H 1 -0.69055 0.10724 . . . . . . . . . . 50234 1 stop_ save_ ######################################## # Heteronuclear T1 relaxation values # ######################################## save_heteronucl_T1_relaxation_1 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode heteronucl_T1_relaxation_1 _Heteronucl_T1_list.Entry_ID 50234 _Heteronucl_T1_list.ID 1 _Heteronucl_T1_list.Name R1-600 _Heteronucl_T1_list.Sample_condition_list_ID 1 _Heteronucl_T1_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T1_list.Spectrometer_frequency_1H 600.4021885 _Heteronucl_T1_list.T1_coherence_type Iz _Heteronucl_T1_list.T1_val_units s-1 _Heteronucl_T1_list.Details . _Heteronucl_T1_list.Text_data_format . _Heteronucl_T1_list.Text_data . loop_ _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_name _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Sample_state _Heteronucl_T1_experiment.Entry_ID _Heteronucl_T1_experiment.Heteronucl_T1_list_ID 1 'T1/R1 relaxation' . . . 50234 1 stop_ loop_ _Heteronucl_T1_software.Software_ID _Heteronucl_T1_software.Software_label _Heteronucl_T1_software.Method_ID _Heteronucl_T1_software.Method_label _Heteronucl_T1_software.Entry_ID _Heteronucl_T1_software.Heteronucl_T1_list_ID 1 $software_1 . . 50234 1 2 $software_2 . . 50234 1 3 $software_3 . . 50234 1 stop_ loop_ _T1.ID _T1.Assembly_atom_ID _T1.Entity_assembly_ID _T1.Entity_ID _T1.Comp_index_ID _T1.Seq_ID _T1.Comp_ID _T1.Atom_ID _T1.Atom_type _T1.Atom_isotope_number _T1.Val _T1.Val_err _T1.Resonance_ID _T1.Auth_entity_assembly_ID _T1.Auth_seq_ID _T1.Auth_comp_ID _T1.Auth_atom_ID _T1.Entry_ID _T1.Heteronucl_T1_list_ID 1 . 1 1 5 5 SER N N 15 1.35254 0.06394 . . . . . 50234 1 2 . 1 1 6 6 GLU N N 15 1.53108 0.04458 . . . . . 50234 1 3 . 1 1 8 8 GLU N N 15 1.55705 0.02712 . . . . . 50234 1 4 . 1 1 11 11 SER N N 15 1.46139 0.0743 . . . . . 50234 1 5 . 1 1 12 12 GLY N N 15 1.4811 0.06771 . . . . . 50234 1 6 . 1 1 13 13 THR N N 15 1.49104 0.07339 . . . . . 50234 1 7 . 1 1 14 14 THR N N 15 1.52311 0.07361 . . . . . 50234 1 8 . 1 1 15 15 THR N N 15 1.54918 0.07619 . . . . . 50234 1 9 . 1 1 16 16 GLY N N 15 1.52424 0.07232 . . . . . 50234 1 10 . 1 1 19 19 LEU N N 15 1.47241 0.03779 . . . . . 50234 1 11 . 1 1 21 21 SER N N 15 1.58188 0.04766 . . . . . 50234 1 12 . 1 1 27 27 ALA N N 15 1.50095 0.0363 . . . . . 50234 1 13 . 1 1 28 28 ALA N N 15 1.47363 0.0535 . . . . . 50234 1 14 . 1 1 29 29 ASN N N 15 1.40771 0.04885 . . . . . 50234 1 15 . 1 1 31 31 GLY N N 15 1.40433 0.05805 . . . . . 50234 1 16 . 1 1 33 33 THR N N 15 1.34182 0.03509 . . . . . 50234 1 17 . 1 1 36 36 SER N N 15 1.38126 0.03103 . . . . . 50234 1 18 . 1 1 37 37 LEU N N 15 1.26505 0.04845 . . . . . 50234 1 19 . 1 1 39 39 GLN N N 15 1.41314 0.04282 . . . . . 50234 1 20 . 1 1 42 42 GLY N N 15 1.31463 0.03629 . . . . . 50234 1 21 . 1 1 44 44 ARG N N 15 1.33413 0.03177 . . . . . 50234 1 22 . 1 1 45 45 ILE N N 15 1.2286 0.04182 . . . . . 50234 1 23 . 1 1 46 46 GLY N N 15 1.38252 0.04472 . . . . . 50234 1 24 . 1 1 47 47 LYS N N 15 1.5712 0.03164 . . . . . 50234 1 25 . 1 1 51 51 TYR N N 15 1.29668 0.03415 . . . . . 50234 1 26 . 1 1 52 52 ASP N N 15 1.28117 0.0383 . . . . . 50234 1 27 . 1 1 53 53 LEU N N 15 1.35183 0.0387 . . . . . 50234 1 28 . 1 1 54 54 LEU N N 15 1.32112 0.05763 . . . . . 50234 1 29 . 1 1 56 56 ALA N N 15 1.33665 0.0385 . . . . . 50234 1 30 . 1 1 57 57 GLU N N 15 1.32171 0.04039 . . . . . 50234 1 31 . 1 1 59 59 GLN N N 15 1.45646 0.06098 . . . . . 50234 1 32 . 1 1 64 64 ASN N N 15 1.32544 0.03434 . . . . . 50234 1 33 . 1 1 65 65 PHE N N 15 1.23591 0.04152 . . . . . 50234 1 34 . 1 1 66 66 THR N N 15 1.24011 0.03103 . . . . . 50234 1 35 . 1 1 67 67 PHE N N 15 1.36333 0.03943 . . . . . 50234 1 36 . 1 1 68 68 ARG N N 15 1.33993 0.0547 . . . . . 50234 1 37 . 1 1 69 69 VAL N N 15 1.37763 0.09203 . . . . . 50234 1 38 . 1 1 70 70 THR N N 15 1.38221 0.04527 . . . . . 50234 1 39 . 1 1 71 71 VAL N N 15 1.29548 0.09437 . . . . . 50234 1 40 . 1 1 72 72 GLY N N 15 1.33606 0.04235 . . . . . 50234 1 41 . 1 1 73 73 ASP N N 15 1.24795 0.05555 . . . . . 50234 1 42 . 1 1 74 74 THR N N 15 1.32421 0.05067 . . . . . 50234 1 43 . 1 1 76 76 CYS N N 15 1.36354 0.04156 . . . . . 50234 1 44 . 1 1 78 78 GLY N N 15 1.30099 0.03867 . . . . . 50234 1 45 . 1 1 80 80 GLY N N 15 1.31634 0.01559 . . . . . 50234 1 46 . 1 1 83 83 LYS N N 15 1.55134 0.05371 . . . . . 50234 1 47 . 1 1 85 85 ALA N N 15 1.31923 0.0667 . . . . . 50234 1 48 . 1 1 91 91 ALA N N 15 1.37174 0.0726 . . . . . 50234 1 49 . 1 1 92 92 GLU N N 15 1.23176 0.03046 . . . . . 50234 1 50 . 1 1 93 93 VAL N N 15 1.43155 0.05881 . . . . . 50234 1 51 . 1 1 97 97 HIS N N 15 1.55786 0.04367 . . . . . 50234 1 52 . 1 1 98 98 LEU N N 15 1.60455 0.0388 . . . . . 50234 1 53 . 1 1 100 100 GLY N N 15 1.51597 0.03841 . . . . . 50234 1 54 . 1 1 101 101 GLY N N 15 1.63344 0.06512 . . . . . 50234 1 55 . 1 1 102 102 SER N N 15 1.57869 0.07199 . . . . . 50234 1 56 . 1 1 104 104 LEU N N 15 1.60321 0.03286 . . . . . 50234 1 57 . 1 1 107 107 ALA N N 15 1.30939 0.02692 . . . . . 50234 1 58 . 1 1 108 108 LEU N N 15 0.97581 0.00686 . . . . . 50234 1 stop_ save_ ######################################## # Heteronuclear T2 relaxation values # ######################################## save_heteronucl_T2_relaxation_1 _Heteronucl_T2_list.Sf_category heteronucl_T2_relaxation _Heteronucl_T2_list.Sf_framecode heteronucl_T2_relaxation_1 _Heteronucl_T2_list.Entry_ID 50234 _Heteronucl_T2_list.ID 1 _Heteronucl_T2_list.Name R2-600 _Heteronucl_T2_list.Sample_condition_list_ID 1 _Heteronucl_T2_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T2_list.Temp_calibration_method 'no calibration applied' _Heteronucl_T2_list.Temp_control_method 'single scan interleaving' _Heteronucl_T2_list.Spectrometer_frequency_1H 600.4021885 _Heteronucl_T2_list.T2_coherence_type I(+,-) _Heteronucl_T2_list.T2_val_units s-1 _Heteronucl_T2_list.Rex_units . _Heteronucl_T2_list.Details . _Heteronucl_T2_list.Text_data_format . _Heteronucl_T2_list.Text_data . loop_ _Heteronucl_T2_experiment.Experiment_ID _Heteronucl_T2_experiment.Experiment_name _Heteronucl_T2_experiment.Sample_ID _Heteronucl_T2_experiment.Sample_label _Heteronucl_T2_experiment.Sample_state _Heteronucl_T2_experiment.Entry_ID _Heteronucl_T2_experiment.Heteronucl_T2_list_ID 2 'T2/R2 relaxation' . . . 50234 1 stop_ loop_ _Heteronucl_T2_software.Software_ID _Heteronucl_T2_software.Software_label _Heteronucl_T2_software.Method_ID _Heteronucl_T2_software.Method_label _Heteronucl_T2_software.Entry_ID _Heteronucl_T2_software.Heteronucl_T2_list_ID 1 $software_1 . . 50234 1 2 $software_2 . . 50234 1 3 $software_3 . . 50234 1 stop_ loop_ _T2.ID _T2.Assembly_atom_ID _T2.Entity_assembly_ID _T2.Entity_ID _T2.Comp_index_ID _T2.Seq_ID _T2.Comp_ID _T2.Atom_ID _T2.Atom_type _T2.Atom_isotope_number _T2.T2_val _T2.T2_val_err _T2.Rex_val _T2.Rex_err _T2.Resonance_ID _T2.Auth_entity_assembly_ID _T2.Auth_seq_ID _T2.Auth_comp_ID _T2.Auth_atom_ID _T2.Entry_ID _T2.Heteronucl_T2_list_ID 1 . 1 1 5 5 SER N N 15 1.74480 0.20988 . . . . . . . 50234 1 2 . 1 1 6 6 GLU N N 15 1.95965 0.20875 . . . . . . . 50234 1 3 . 1 1 8 8 GLU N N 15 2.14699 0.20311 . . . . . . . 50234 1 4 . 1 1 11 11 SER N N 15 2.12644 0.28258 . . . . . . . 50234 1 5 . 1 1 12 12 GLY N N 15 2.07268 0.28561 . . . . . . . 50234 1 6 . 1 1 13 13 THR N N 15 2.10188 0.24780 . . . . . . . 50234 1 7 . 1 1 14 14 THR N N 15 2.47827 0.33643 . . . . . . . 50234 1 8 . 1 1 15 15 THR N N 15 2.50521 0.30880 . . . . . . . 50234 1 9 . 1 1 16 16 GLY N N 15 2.49669 0.28523 . . . . . . . 50234 1 10 . 1 1 19 19 LEU N N 15 3.70077 0.29869 . . . . . . . 50234 1 11 . 1 1 21 21 SER N N 15 6.17225 0.64926 . . . . . . . 50234 1 12 . 1 1 27 27 ALA N N 15 7.39557 0.71688 . . . . . . . 50234 1 13 . 1 1 28 28 ALA N N 15 8.82112 0.86492 . . . . . . . 50234 1 14 . 1 1 29 29 ASN N N 15 6.87714 0.68072 . . . . . . . 50234 1 15 . 1 1 31 31 GLY N N 15 6.22731 0.81961 . . . . . . . 50234 1 16 . 1 1 33 33 THR N N 15 8.76133 0.80633 . . . . . . . 50234 1 17 . 1 1 36 36 SER N N 15 11.04404 1.15027 . . . . . . . 50234 1 18 . 1 1 37 37 LEU N N 15 11.14659 1.19139 . . . . . . . 50234 1 19 . 1 1 39 39 GLN N N 15 9.87293 1.10731 . . . . . . . 50234 1 20 . 1 1 42 42 GLY N N 15 12.81152 1.36917 . . . . . . . 50234 1 21 . 1 1 44 44 ARG N N 15 10.55686 0.94856 . . . . . . . 50234 1 22 . 1 1 45 45 ILE N N 15 10.78864 1.24174 . . . . . . . 50234 1 23 . 1 1 46 46 GLY N N 15 9.54457 0.88676 . . . . . . . 50234 1 24 . 1 1 47 47 LYS N N 15 5.10902 0.52385 . . . . . . . 50234 1 25 . 1 1 51 51 TYR N N 15 10.67188 1.01707 . . . . . . . 50234 1 26 . 1 1 52 52 ASP N N 15 9.27643 0.92030 . . . . . . . 50234 1 27 . 1 1 53 53 LEU N N 15 9.93967 1.20446 . . . . . . . 50234 1 28 . 1 1 54 54 LEU N N 15 10.79863 1.41971 . . . . . . . 50234 1 29 . 1 1 56 56 ALA N N 15 8.52870 0.87346 . . . . . . . 50234 1 30 . 1 1 57 57 GLU N N 15 6.01576 0.59761 . . . . . . . 50234 1 31 . 1 1 59 59 GLN N N 15 5.13607 0.48199 . . . . . . . 50234 1 32 . 1 1 64 64 ASN N N 15 8.15471 0.77532 . . . . . . . 50234 1 33 . 1 1 65 65 PHE N N 15 9.91685 1.04606 . . . . . . . 50234 1 34 . 1 1 66 66 THR N N 15 9.89490 1.14201 . . . . . . . 50234 1 35 . 1 1 67 67 PHE N N 15 10.04049 0.97343 . . . . . . . 50234 1 36 . 1 1 68 68 ARG N N 15 9.63678 0.96134 . . . . . . . 50234 1 37 . 1 1 69 69 VAL N N 15 10.51986 1.31248 . . . . . . . 50234 1 38 . 1 1 70 70 THR N N 15 8.97613 0.92863 . . . . . . . 50234 1 39 . 1 1 71 71 VAL N N 15 9.92778 0.97040 . . . . . . . 50234 1 40 . 1 1 72 72 GLY N N 15 9.82237 1.02440 . . . . . . . 50234 1 41 . 1 1 73 73 ASP N N 15 10.15966 1.04511 . . . . . . . 50234 1 42 . 1 1 74 74 THR N N 15 9.44014 0.93584 . . . . . . . 50234 1 43 . 1 1 76 76 CYS N N 15 9.30729 1.03672 . . . . . . . 50234 1 44 . 1 1 78 78 GLY N N 15 12.31316 1.37173 . . . . . . . 50234 1 45 . 1 1 80 80 GLY N N 15 12.15888 1.31272 . . . . . . . 50234 1 46 . 1 1 83 83 LYS N N 15 7.82043 0.90340 . . . . . . . 50234 1 47 . 1 1 85 85 ALA N N 15 11.22303 1.31290 . . . . . . . 50234 1 48 . 1 1 91 91 ALA N N 15 14.06307 1.49234 . . . . . . . 50234 1 49 . 1 1 92 92 GLU N N 15 12.58844 1.43749 . . . . . . . 50234 1 50 . 1 1 93 93 VAL N N 15 9.88012 1.36166 . . . . . . . 50234 1 51 . 1 1 97 97 HIS N N 15 7.45741 0.75789 . . . . . . . 50234 1 52 . 1 1 98 98 LEU N N 15 2.99494 0.34275 . . . . . . . 50234 1 53 . 1 1 100 100 GLY N N 15 9.21907 0.98778 . . . . . . . 50234 1 54 . 1 1 101 101 GLY N N 15 6.18464 0.59931 . . . . . . . 50234 1 55 . 1 1 102 102 SER N N 15 5.00296 0.52276 . . . . . . . 50234 1 56 . 1 1 104 104 LEU N N 15 4.06987 0.40867 . . . . . . . 50234 1 57 . 1 1 107 107 ALA N N 15 1.96287 0.18738 . . . . . . . 50234 1 58 . 1 1 108 108 LEU N N 15 2.25384 0.18876 . . . . . . . 50234 1 stop_ save_