data_50257 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; hnRNPA2 1-189 bound to rA2RE11 ; _BMRB_accession_number 50257 _BMRB_flat_file_name bmr50257.str _Entry_type original _Submission_date 2020-05-01 _Accession_date 2020-05-01 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Ryan Veronica . . 2 Watters Scott . . 3 Naik Mandar . . 4 Fawzi Nicolas . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 164 "15N chemical shifts" 164 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-10-16 update BMRB 'update entry citation' 2020-08-20 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 50254 'hnRNPA2 1-189' 50255 'hnRNPA2 1-189 310K' stop_ _Original_release_date 2020-05-01 save_ ############################# # Citation for this entry # ############################# save_citations_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Weak binding to the A2RE RNA rigidifies hnRNPA2 RRMs and reduces liquid-liquid phase separation and aggregation ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 32870271 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Ryan Veronica . . 2 Watters Scott . . 3 Amaya Joshua . . 4 Khatiwada Balabhadra . . 5 Vendetti Vincenzo . . 6 Naik Mandar . . 7 Fawzi Nicolas . . stop_ _Journal_abbreviation 'Nucleic Acids Res' _Journal_name_full 'Nucleic acids research' _Journal_volume 48 _Journal_issue 18 _Journal_ISSN 1362-4962 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 10542 _Page_last 10554 _Year 2020 _Details . save_ ################################## # Molecular system description # ################################## save_assembly_1 _Saveframe_category molecular_system _Mol_system_name 'assembly 1' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'hnRNPA2 1-189' $entity_1 rA2RE11 $entity_2 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common entity_1 _Molecular_mass . _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 189 _Mol_residue_sequence ; MEREKEQFRKLFIGGLSFET TEESLRNYYEQWGKLTDCVV MRDPASKRSRGFGFVTFSSM AEVDAAMAARPHSIDGRVVE PKRAVAREESGKPGAHVTVK KLFVGGIKEDTEEHHLRDYF EEYGKIDTIEIITDRQSGKK RGFGFVTFDDHDPVDKIVLQ KYHTINGHNAEVRKALSRQE MQEVQSSRS ; loop_ _Residue_seq_code _Residue_label 1 MET 2 GLU 3 ARG 4 GLU 5 LYS 6 GLU 7 GLN 8 PHE 9 ARG 10 LYS 11 LEU 12 PHE 13 ILE 14 GLY 15 GLY 16 LEU 17 SER 18 PHE 19 GLU 20 THR 21 THR 22 GLU 23 GLU 24 SER 25 LEU 26 ARG 27 ASN 28 TYR 29 TYR 30 GLU 31 GLN 32 TRP 33 GLY 34 LYS 35 LEU 36 THR 37 ASP 38 CYS 39 VAL 40 VAL 41 MET 42 ARG 43 ASP 44 PRO 45 ALA 46 SER 47 LYS 48 ARG 49 SER 50 ARG 51 GLY 52 PHE 53 GLY 54 PHE 55 VAL 56 THR 57 PHE 58 SER 59 SER 60 MET 61 ALA 62 GLU 63 VAL 64 ASP 65 ALA 66 ALA 67 MET 68 ALA 69 ALA 70 ARG 71 PRO 72 HIS 73 SER 74 ILE 75 ASP 76 GLY 77 ARG 78 VAL 79 VAL 80 GLU 81 PRO 82 LYS 83 ARG 84 ALA 85 VAL 86 ALA 87 ARG 88 GLU 89 GLU 90 SER 91 GLY 92 LYS 93 PRO 94 GLY 95 ALA 96 HIS 97 VAL 98 THR 99 VAL 100 LYS 101 LYS 102 LEU 103 PHE 104 VAL 105 GLY 106 GLY 107 ILE 108 LYS 109 GLU 110 ASP 111 THR 112 GLU 113 GLU 114 HIS 115 HIS 116 LEU 117 ARG 118 ASP 119 TYR 120 PHE 121 GLU 122 GLU 123 TYR 124 GLY 125 LYS 126 ILE 127 ASP 128 THR 129 ILE 130 GLU 131 ILE 132 ILE 133 THR 134 ASP 135 ARG 136 GLN 137 SER 138 GLY 139 LYS 140 LYS 141 ARG 142 GLY 143 PHE 144 GLY 145 PHE 146 VAL 147 THR 148 PHE 149 ASP 150 ASP 151 HIS 152 ASP 153 PRO 154 VAL 155 ASP 156 LYS 157 ILE 158 VAL 159 LEU 160 GLN 161 LYS 162 TYR 163 HIS 164 THR 165 ILE 166 ASN 167 GLY 168 HIS 169 ASN 170 ALA 171 GLU 172 VAL 173 ARG 174 LYS 175 ALA 176 LEU 177 SER 178 ARG 179 GLN 180 GLU 181 MET 182 GLN 183 GLU 184 VAL 185 GLN 186 SER 187 SER 188 ARG 189 SER stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ save_entity_2 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common entity_2 _Molecular_mass . _Mol_thiol_state 'not present' _Details . _Residue_count 11 _Mol_residue_sequence ; GCCAAGGAGCC ; loop_ _Residue_seq_code _Residue_label 1 G 2 C 3 C 4 A 5 A 6 G 7 G 8 A 9 G 10 C 11 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source_1 _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 Human 9606 Eukaryota Metazoa Homo sapiens $entity_2 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name $entity_1 'recombinant technology' . . . . plasmid pJ411 $entity_2 'obtained from a vendor' . . . . plasmid . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 200 uM '[U-99% 15N]' $entity_2 600 uM 'natural abundance' D2O 10 % '[U-99% 2H]' NaCl 150 mM 'natural abundance' NaPi 20 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name NMRPipe _Version . loop_ _Task 'data analysis' stop_ _Details . save_ save_software_2 _Saveframe_category software _Name NMRFAM-SPARKY _Version . loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 850 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.8 . pH pressure 1 . atm temperature 310 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0 internal direct . . . 1 DSS N 15 'methyl protons' ppm 0 na indirect . . . . stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $software_1 $software_2 stop_ loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name 'hnRNPA2 1-189' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 6 6 GLU H H 9.723 0.00 1 2 6 6 GLU N N 129.304 0.00 1 3 7 7 GLN H H 8.552 0.00 1 4 7 7 GLN N N 116.057 0.00 1 5 8 8 PHE H H 7.626 0.00 1 6 8 8 PHE N N 116.402 0.00 1 7 9 9 ARG H H 7.815 0.00 1 8 9 9 ARG N N 117.066 0.00 1 9 10 10 LYS H H 7.090 0.00 1 10 10 10 LYS N N 120.769 0.00 1 11 11 11 LEU H H 9.520 0.00 1 12 11 11 LEU N N 126.002 0.00 1 13 12 12 PHE H H 9.229 0.00 1 14 12 12 PHE N N 123.934 0.00 1 15 13 13 ILE H H 8.625 0.00 1 16 13 13 ILE N N 126.395 0.00 1 17 14 14 GLY H H 7.922 0.00 1 18 14 14 GLY N N 112.639 0.00 1 19 15 15 GLY H H 8.522 0.00 1 20 15 15 GLY N N 107.558 0.00 1 21 16 16 LEU H H 7.541 0.00 1 22 16 16 LEU N N 115.764 0.00 1 23 17 17 SER H H 9.051 0.00 1 24 17 17 SER N N 115.068 0.00 1 25 18 18 PHE H H 9.006 0.00 1 26 18 18 PHE N N 128.047 0.00 1 27 19 19 GLU H H 8.257 0.00 1 28 19 19 GLU N N 115.882 0.00 1 29 20 20 THR H H 7.347 0.00 1 30 20 20 THR N N 118.541 0.00 1 31 21 21 THR H H 8.059 0.00 1 32 21 21 THR N N 119.024 0.00 1 33 22 22 GLU H H 9.938 0.00 1 34 22 22 GLU N N 120.891 0.00 1 35 23 23 GLU H H 8.227 0.00 1 36 23 23 GLU N N 117.819 0.00 1 37 24 24 SER H H 7.732 0.00 1 38 24 24 SER N N 116.269 0.00 1 39 25 25 LEU H H 8.279 0.00 1 40 25 25 LEU N N 124.152 0.00 1 41 26 26 ARG H H 8.366 0.00 1 42 26 26 ARG N N 119.772 0.00 1 43 27 27 ASN H H 8.178 0.00 1 44 27 27 ASN N N 114.831 0.00 1 45 28 28 TYR H H 7.692 0.00 1 46 28 28 TYR N N 117.489 0.00 1 47 29 29 TYR H H 8.276 0.00 1 48 29 29 TYR N N 116.097 0.00 1 49 30 30 GLU H H 8.401 0.00 1 50 30 30 GLU N N 117.742 0.00 1 51 31 31 GLN H H 6.928 0.00 1 52 31 31 GLN N N 116.996 0.00 1 53 32 32 TRP H H 7.603 0.00 1 54 32 32 TRP N N 115.957 0.00 1 55 33 33 GLY H H 7.332 0.00 1 56 33 33 GLY N N 105.233 0.00 1 57 34 34 LYS H H 8.328 0.00 1 58 34 34 LYS N N 119.816 0.00 1 59 35 35 LEU H H 8.958 0.00 1 60 35 35 LEU N N 124.316 0.00 1 61 36 36 THR H H 9.019 0.00 1 62 36 36 THR N N 115.008 0.00 1 63 37 37 ASP H H 7.567 0.00 1 64 37 37 ASP N N 119.025 0.00 1 65 38 38 CYS H H 8.332 0.00 1 66 38 38 CYS N N 122.592 0.00 1 67 39 39 VAL H H 8.795 0.00 1 68 39 39 VAL N N 127.198 0.00 1 69 40 40 VAL H H 8.217 0.00 1 70 40 40 VAL N N 125.411 0.00 1 71 41 41 MET H H 8.400 0.00 1 72 41 41 MET N N 126.300 0.00 1 73 42 42 ARG H H 8.283 0.00 1 74 42 42 ARG N N 121.063 0.00 1 75 43 43 ASP H H 8.677 0.00 1 76 43 43 ASP N N 124.786 0.00 1 77 45 45 ALA H H 8.183 0.00 1 78 45 45 ALA N N 119.506 0.00 1 79 46 46 SER H H 8.122 0.00 1 80 46 46 SER N N 111.968 0.00 1 81 47 47 LYS H H 8.228 0.00 1 82 47 47 LYS N N 116.603 0.00 1 83 48 48 ARG H H 7.918 0.00 1 84 48 48 ARG N N 120.450 0.00 1 85 49 49 SER H H 8.779 0.00 1 86 49 49 SER N N 116.788 0.00 1 87 50 50 ARG H H 8.993 0.00 1 88 50 50 ARG N N 123.105 0.00 1 89 51 51 GLY H H 9.432 0.00 1 90 51 51 GLY N N 107.545 0.00 1 91 52 52 PHE H H 7.117 0.00 1 92 52 52 PHE N N 112.646 0.00 1 93 53 53 GLY H H 8.431 0.00 1 94 53 53 GLY N N 106.920 0.00 1 95 54 54 PHE H H 9.047 0.00 1 96 54 54 PHE N N 115.382 0.00 1 97 55 55 VAL H H 8.745 0.00 1 98 55 55 VAL N N 114.536 0.00 1 99 56 56 THR H H 8.100 0.00 1 100 56 56 THR N N 120.074 0.00 1 101 57 57 PHE H H 8.860 0.00 1 102 57 57 PHE N N 125.586 0.00 1 103 58 58 SER H H 9.180 0.00 1 104 58 58 SER N N 116.923 0.00 1 105 59 59 SER H H 8.130 0.00 1 106 59 59 SER N N 114.375 0.00 1 107 60 60 MET H H 9.490 0.00 1 108 60 60 MET N N 123.979 0.00 1 109 61 61 ALA H H 8.403 0.00 1 110 61 61 ALA N N 121.218 0.00 1 111 62 62 GLU H H 7.344 0.00 1 112 62 62 GLU N N 118.980 0.00 1 113 63 63 VAL H H 6.713 0.00 1 114 63 63 VAL N N 117.834 0.00 1 115 64 64 ASP H H 8.099 0.00 1 116 64 64 ASP N N 118.747 0.00 1 117 65 65 ALA H H 7.665 0.00 1 118 65 65 ALA N N 123.717 0.00 1 119 66 66 ALA H H 7.747 0.00 1 120 66 66 ALA N N 120.847 0.00 1 121 67 67 MET H H 8.560 0.00 1 122 67 67 MET N N 115.743 0.00 1 123 68 68 ALA H H 7.782 0.00 1 124 68 68 ALA N N 123.061 0.00 1 125 69 69 ALA H H 7.039 0.00 1 126 69 69 ALA N N 120.877 0.00 1 127 70 70 ARG H H 6.558 0.00 1 128 70 70 ARG N N 118.581 0.00 1 129 72 72 HIS H H 9.467 0.00 1 130 72 72 HIS N N 124.049 0.00 1 131 73 73 SER H H 7.521 0.00 1 132 73 73 SER N N 116.537 0.00 1 133 74 74 ILE H H 8.395 0.00 1 134 74 74 ILE N N 123.061 0.00 1 135 75 75 ASP H H 9.315 0.00 1 136 75 75 ASP N N 127.435 0.00 1 137 76 76 GLY H H 8.503 0.00 1 138 76 76 GLY N N 102.365 0.00 1 139 77 77 ARG H H 7.504 0.00 1 140 77 77 ARG N N 119.830 0.00 1 141 78 78 VAL H H 8.373 0.00 1 142 78 78 VAL N N 124.022 0.00 1 143 79 79 VAL H H 8.143 0.00 1 144 79 79 VAL N N 122.993 0.00 1 145 80 80 GLU H H 8.548 0.00 1 146 80 80 GLU N N 119.609 0.00 1 147 82 82 LYS H H 9.556 0.00 1 148 82 82 LYS N N 120.518 0.00 1 149 84 84 ALA H H 8.186 0.00 1 150 84 84 ALA N N 125.360 0.00 1 151 85 85 VAL H H 7.736 0.00 1 152 85 85 VAL N N 115.846 0.00 1 153 86 86 ALA H H 8.252 0.00 1 154 86 86 ALA N N 126.827 0.00 1 155 87 87 ARG H H 8.339 0.00 1 156 87 87 ARG N N 121.015 0.00 1 157 88 88 GLU H H 8.207 0.00 1 158 88 88 GLU N N 120.896 0.00 1 159 89 89 GLU H H 8.311 0.00 1 160 89 89 GLU N N 121.521 0.00 1 161 90 90 SER H H 8.155 0.00 1 162 90 90 SER N N 116.074 0.00 1 163 91 91 GLY H H 8.505 0.00 1 164 91 91 GLY N N 110.778 0.00 1 165 92 92 LYS H H 7.861 0.00 1 166 92 92 LYS N N 121.577 0.00 1 167 94 94 GLY H H 8.490 0.00 1 168 94 94 GLY N N 109.467 0.00 1 169 95 95 ALA H H 7.909 0.00 1 170 95 95 ALA N N 122.938 0.00 1 171 96 96 HIS H H 8.261 0.00 1 172 96 96 HIS N N 117.143 0.00 1 173 97 97 VAL H H 7.679 0.00 1 174 97 97 VAL N N 121.069 0.00 1 175 98 98 THR H H 8.354 0.00 1 176 98 98 THR N N 119.986 0.00 1 177 99 99 VAL H H 8.499 0.00 1 178 99 99 VAL N N 121.679 0.00 1 179 100 100 LYS H H 8.565 0.00 1 180 100 100 LYS N N 120.363 0.00 1 181 102 102 LEU H H 9.392 0.00 1 182 102 102 LEU N N 127.491 0.00 1 183 103 103 PHE H H 9.240 0.00 1 184 103 103 PHE N N 126.776 0.00 1 185 104 104 VAL H H 8.012 0.00 1 186 104 104 VAL N N 127.284 0.00 1 187 105 105 GLY H H 9.163 0.00 1 188 105 105 GLY N N 113.044 0.00 1 189 106 106 GLY H H 8.449 0.00 1 190 106 106 GLY N N 106.903 0.00 1 191 110 110 ASP H H 8.229 0.00 1 192 110 110 ASP N N 117.046 0.00 1 193 112 112 GLU H H 11.279 0.00 1 194 112 112 GLU N N 130.210 0.00 1 195 113 113 GLU H H 9.453 0.00 1 196 113 113 GLU N N 121.933 0.00 1 197 114 114 HIS H H 8.164 0.00 1 198 114 114 HIS N N 113.490 0.00 1 199 115 115 HIS H H 6.595 0.00 1 200 115 115 HIS N N 118.097 0.00 1 201 116 116 LEU H H 7.176 0.00 1 202 116 116 LEU N N 116.642 0.00 1 203 117 117 ARG H H 8.631 0.00 1 204 117 117 ARG N N 121.852 0.00 1 205 118 118 ASP H H 8.355 0.00 1 206 118 118 ASP N N 116.124 0.00 1 207 119 119 TYR H H 7.325 0.00 1 208 119 119 TYR N N 116.425 0.00 1 209 120 120 PHE H H 8.580 0.00 1 210 120 120 PHE N N 112.634 0.00 1 211 121 121 GLU H H 8.790 0.00 1 212 121 121 GLU N N 121.306 0.00 1 213 122 122 GLU H H 7.272 0.00 1 214 122 122 GLU N N 115.772 0.00 1 215 123 123 TYR H H 8.137 0.00 1 216 123 123 TYR N N 115.344 0.00 1 217 124 124 GLY H H 7.587 0.00 1 218 124 124 GLY N N 105.601 0.00 1 219 125 125 LYS H H 8.331 0.00 1 220 125 125 LYS N N 118.433 0.00 1 221 126 126 ILE H H 8.746 0.00 1 222 126 126 ILE N N 128.165 0.00 1 223 127 127 ASP H H 9.148 0.00 1 224 127 127 ASP N N 128.595 0.00 1 225 128 128 THR H H 7.404 0.00 1 226 128 128 THR N N 108.747 0.00 1 227 129 129 ILE H H 8.436 0.00 1 228 129 129 ILE N N 123.004 0.00 1 229 130 130 GLU H H 8.974 0.00 1 230 130 130 GLU N N 126.889 0.00 1 231 131 131 ILE H H 8.872 0.00 1 232 131 131 ILE N N 126.186 0.00 1 233 132 132 ILE H H 7.782 0.00 1 234 132 132 ILE N N 127.793 0.00 1 235 133 133 THR H H 8.386 0.00 1 236 133 133 THR N N 119.764 0.00 1 237 134 134 ASP H H 8.827 0.00 1 238 134 134 ASP N N 123.552 0.00 1 239 135 135 ARG H H 8.978 0.00 1 240 135 135 ARG N N 127.794 0.00 1 241 136 136 GLN H H 8.460 0.00 1 242 136 136 GLN N N 117.189 0.00 1 243 137 137 SER H H 8.267 0.00 1 244 137 137 SER N N 112.427 0.00 1 245 138 138 GLY H H 8.287 0.00 1 246 138 138 GLY N N 112.418 0.00 1 247 139 139 LYS H H 7.759 0.00 1 248 139 139 LYS N N 119.902 0.00 1 249 140 140 LYS H H 8.937 0.00 1 250 140 140 LYS N N 124.459 0.00 1 251 142 142 GLY H H 9.131 0.00 1 252 142 142 GLY N N 109.279 0.00 1 253 143 143 PHE H H 7.337 0.00 1 254 143 143 PHE N N 113.592 0.00 1 255 144 144 GLY H H 8.670 0.00 1 256 144 144 GLY N N 106.404 0.00 1 257 145 145 PHE H H 8.970 0.00 1 258 145 145 PHE N N 113.498 0.00 1 259 147 147 THR H H 8.940 0.00 1 260 147 147 THR N N 123.077 0.00 1 261 148 148 PHE H H 9.082 0.00 1 262 148 148 PHE N N 127.224 0.00 1 263 149 149 ASP H H 8.235 0.00 1 264 149 149 ASP N N 118.803 0.00 1 265 150 150 ASP H H 7.940 0.00 1 266 150 150 ASP N N 117.654 0.00 1 267 151 151 HIS H H 8.003 0.00 1 268 151 151 HIS N N 116.320 0.00 1 269 152 152 ASP H H 7.909 0.00 1 270 152 152 ASP N N 122.645 0.00 1 271 155 155 ASP H H 7.545 0.00 1 272 155 155 ASP N N 117.893 0.00 1 273 157 157 ILE H H 8.370 0.00 1 274 157 157 ILE N N 121.146 0.00 1 275 158 158 VAL H H 7.969 0.00 1 276 158 158 VAL N N 112.297 0.00 1 277 159 159 LEU H H 7.445 0.00 1 278 159 159 LEU N N 121.545 0.00 1 279 160 160 GLN H H 7.723 0.00 1 280 160 160 GLN N N 118.281 0.00 1 281 163 163 HIS H H 8.931 0.00 1 282 163 163 HIS N N 123.385 0.00 1 283 164 164 THR H H 8.802 0.00 1 284 164 164 THR N N 121.799 0.00 1 285 165 165 ILE H H 8.404 0.00 1 286 165 165 ILE N N 125.557 0.00 1 287 166 166 ASN H H 9.559 0.00 1 288 166 166 ASN N N 127.103 0.00 1 289 167 167 GLY H H 8.183 0.00 1 290 167 167 GLY N N 100.384 0.00 1 291 168 168 HIS H H 7.739 0.00 1 292 168 168 HIS N N 119.040 0.00 1 293 170 170 ALA H H 8.817 0.00 1 294 170 170 ALA N N 131.157 0.00 1 295 171 171 GLU H H 8.136 0.00 1 296 171 171 GLU N N 122.501 0.00 1 297 172 172 VAL H H 8.509 0.00 1 298 172 172 VAL N N 118.884 0.00 1 299 173 173 ARG H H 8.747 0.00 1 300 173 173 ARG N N 118.420 0.00 1 301 174 174 LYS H H 8.958 0.00 1 302 174 174 LYS N N 121.713 0.00 1 303 175 175 ALA H H 8.354 0.00 1 304 175 175 ALA N N 126.822 0.00 1 305 176 176 LEU H H 7.963 0.00 1 306 176 176 LEU N N 122.611 0.00 1 307 177 177 SER H H 8.615 0.00 1 308 177 177 SER N N 117.175 0.00 1 309 179 179 GLN H H 8.416 0.00 1 310 179 179 GLN N N 120.517 0.00 1 311 180 180 GLU H H 8.431 0.00 1 312 180 180 GLU N N 121.799 0.00 1 313 182 182 GLN H H 8.441 0.00 1 314 182 182 GLN N N 122.225 0.00 1 315 183 183 GLU H H 8.598 0.00 1 316 183 183 GLU N N 121.305 0.00 1 317 184 184 VAL H H 8.033 0.00 1 318 184 184 VAL N N 120.442 0.00 1 319 185 185 GLN H H 8.335 0.00 1 320 185 185 GLN N N 123.336 0.00 1 321 186 186 SER H H 8.257 0.00 1 322 186 186 SER N N 116.955 0.00 1 323 187 187 SER H H 8.253 0.00 1 324 187 187 SER N N 117.888 0.00 1 325 188 188 ARG H H 8.272 0.00 1 326 188 188 ARG N N 123.002 0.00 1 327 189 189 SER H H 7.926 0.00 1 328 189 189 SER N N 122.585 0.00 1 stop_ save_