data_50846 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 50846 _Entry.Title ; Imino resonance assignment of murine Ox40 mRNA 3'UTR ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2021-03-24 _Entry.Accession_date 2021-03-24 _Entry.Last_release_date 2021-03-24 _Entry.Original_release_date 2021-03-24 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details "Assignment of 1H and 15N imino resonances of four fragments of Ox40 mRNA 3'UTR that cover the full-length UTR." _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Jan-Niklas Tants . . . 0000-0002-2455-8135 50846 2 Andreas Schlundt . . . 0000-0003-2254-7560 50846 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 50846 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '15N chemical shifts' 33 50846 '1H chemical shifts' 35 50846 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 2 . . 2022-06-02 2021-03-24 update BMRB 'update entry citation' 50846 1 . . 2022-03-15 2021-03-24 original author 'original release' 50846 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 50846 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID 35357505 _Citation.DOI . _Citation.Full_citation . _Citation.Title ; NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Nucleic Acids Res.' _Citation.Journal_name_full . _Citation.Journal_volume 50 _Citation.Journal_issue 7 _Citation.Journal_ASTM . _Citation.Journal_ISSN 1362-4962 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 4083 _Citation.Page_last 4099 _Citation.Year 2022 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Jan-Niklas Tants J. N. . . 50846 1 2 'Lea Marie' Becker L. M. . . 50846 1 3 Francois McNicoll F. . . . 50846 1 4 Michaela Muller-McNicoll M. . . . 50846 1 5 Andreas Schlundt A. . . . 50846 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 50846 _Assembly.ID 1 _Assembly.Name "Ox40 3'UTR" _Assembly.BMRB_code . _Assembly.Number_of_components 1 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states yes _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass 50406 _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 "Ox40 mRNA 3'UTR" 1 $entity_1 . . yes native yes no . . . 50846 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 50846 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polyribonucleotide _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; GCAUUACUACAGGAGUGGAU UUUAUGGGGCACGGACAACC CAUAUCCUGAUGCCUGCCAG UACCCUCCACACCGUUCUAG GUGCUGGGCUGGCUCUGGGC UUUCCUAUGUAUGCUAUGCA UACUACCUGCCUGGUGGUGC UCCUAAUAAACAUGCUA ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states yes _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 157 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . G . 50846 1 2 . C . 50846 1 3 . A . 50846 1 4 . U . 50846 1 5 . U . 50846 1 6 . A . 50846 1 7 . C . 50846 1 8 . U . 50846 1 9 . A . 50846 1 10 . C . 50846 1 11 . A . 50846 1 12 . G . 50846 1 13 . G . 50846 1 14 . A . 50846 1 15 . G . 50846 1 16 . U . 50846 1 17 . G . 50846 1 18 . G . 50846 1 19 . A . 50846 1 20 . U . 50846 1 21 . U . 50846 1 22 . U . 50846 1 23 . U . 50846 1 24 . A . 50846 1 25 . U . 50846 1 26 . G . 50846 1 27 . G . 50846 1 28 . G . 50846 1 29 . G . 50846 1 30 . C . 50846 1 31 . A . 50846 1 32 . C . 50846 1 33 . G . 50846 1 34 . G . 50846 1 35 . A . 50846 1 36 . C . 50846 1 37 . A . 50846 1 38 . A . 50846 1 39 . C . 50846 1 40 . C . 50846 1 41 . C . 50846 1 42 . A . 50846 1 43 . U . 50846 1 44 . A . 50846 1 45 . U . 50846 1 46 . C . 50846 1 47 . C . 50846 1 48 . U . 50846 1 49 . G . 50846 1 50 . A . 50846 1 51 . U . 50846 1 52 . G . 50846 1 53 . C . 50846 1 54 . C . 50846 1 55 . U . 50846 1 56 . G . 50846 1 57 . C . 50846 1 58 . C . 50846 1 59 . A . 50846 1 60 . G . 50846 1 61 . U . 50846 1 62 . A . 50846 1 63 . C . 50846 1 64 . C . 50846 1 65 . C . 50846 1 66 . U . 50846 1 67 . C . 50846 1 68 . C . 50846 1 69 . A . 50846 1 70 . C . 50846 1 71 . A . 50846 1 72 . C . 50846 1 73 . C . 50846 1 74 . G . 50846 1 75 . U . 50846 1 76 . U . 50846 1 77 . C . 50846 1 78 . U . 50846 1 79 . A . 50846 1 80 . G . 50846 1 81 . G . 50846 1 82 . U . 50846 1 83 . G . 50846 1 84 . C . 50846 1 85 . U . 50846 1 86 . G . 50846 1 87 . G . 50846 1 88 . G . 50846 1 89 . C . 50846 1 90 . U . 50846 1 91 . G . 50846 1 92 . G . 50846 1 93 . C . 50846 1 94 . U . 50846 1 95 . C . 50846 1 96 . U . 50846 1 97 . G . 50846 1 98 . G . 50846 1 99 . G . 50846 1 100 . C . 50846 1 101 . U . 50846 1 102 . U . 50846 1 103 . U . 50846 1 104 . C . 50846 1 105 . C . 50846 1 106 . U . 50846 1 107 . A . 50846 1 108 . U . 50846 1 109 . G . 50846 1 110 . U . 50846 1 111 . A . 50846 1 112 . U . 50846 1 113 . G . 50846 1 114 . C . 50846 1 115 . U . 50846 1 116 . A . 50846 1 117 . U . 50846 1 118 . G . 50846 1 119 . C . 50846 1 120 . A . 50846 1 121 . U . 50846 1 122 . A . 50846 1 123 . C . 50846 1 124 . U . 50846 1 125 . A . 50846 1 126 . C . 50846 1 127 . C . 50846 1 128 . U . 50846 1 129 . G . 50846 1 130 . C . 50846 1 131 . C . 50846 1 132 . U . 50846 1 133 . G . 50846 1 134 . G . 50846 1 135 . U . 50846 1 136 . G . 50846 1 137 . G . 50846 1 138 . U . 50846 1 139 . G . 50846 1 140 . C . 50846 1 141 . U . 50846 1 142 . C . 50846 1 143 . C . 50846 1 144 . U . 50846 1 145 . A . 50846 1 146 . A . 50846 1 147 . U . 50846 1 148 . A . 50846 1 149 . A . 50846 1 150 . A . 50846 1 151 . C . 50846 1 152 . A . 50846 1 153 . U . 50846 1 154 . G . 50846 1 155 . C . 50846 1 156 . U . 50846 1 157 . A . 50846 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . G 1 1 50846 1 . C 2 2 50846 1 . A 3 3 50846 1 . U 4 4 50846 1 . U 5 5 50846 1 . A 6 6 50846 1 . C 7 7 50846 1 . U 8 8 50846 1 . A 9 9 50846 1 . C 10 10 50846 1 . A 11 11 50846 1 . G 12 12 50846 1 . G 13 13 50846 1 . A 14 14 50846 1 . G 15 15 50846 1 . U 16 16 50846 1 . G 17 17 50846 1 . G 18 18 50846 1 . A 19 19 50846 1 . U 20 20 50846 1 . U 21 21 50846 1 . U 22 22 50846 1 . U 23 23 50846 1 . A 24 24 50846 1 . U 25 25 50846 1 . G 26 26 50846 1 . G 27 27 50846 1 . G 28 28 50846 1 . G 29 29 50846 1 . C 30 30 50846 1 . A 31 31 50846 1 . C 32 32 50846 1 . G 33 33 50846 1 . G 34 34 50846 1 . A 35 35 50846 1 . C 36 36 50846 1 . A 37 37 50846 1 . A 38 38 50846 1 . C 39 39 50846 1 . C 40 40 50846 1 . C 41 41 50846 1 . A 42 42 50846 1 . U 43 43 50846 1 . A 44 44 50846 1 . U 45 45 50846 1 . C 46 46 50846 1 . C 47 47 50846 1 . U 48 48 50846 1 . G 49 49 50846 1 . A 50 50 50846 1 . U 51 51 50846 1 . G 52 52 50846 1 . C 53 53 50846 1 . C 54 54 50846 1 . U 55 55 50846 1 . G 56 56 50846 1 . C 57 57 50846 1 . C 58 58 50846 1 . A 59 59 50846 1 . G 60 60 50846 1 . U 61 61 50846 1 . A 62 62 50846 1 . C 63 63 50846 1 . C 64 64 50846 1 . C 65 65 50846 1 . U 66 66 50846 1 . C 67 67 50846 1 . C 68 68 50846 1 . A 69 69 50846 1 . C 70 70 50846 1 . A 71 71 50846 1 . C 72 72 50846 1 . C 73 73 50846 1 . G 74 74 50846 1 . U 75 75 50846 1 . U 76 76 50846 1 . C 77 77 50846 1 . U 78 78 50846 1 . A 79 79 50846 1 . G 80 80 50846 1 . G 81 81 50846 1 . U 82 82 50846 1 . G 83 83 50846 1 . C 84 84 50846 1 . U 85 85 50846 1 . G 86 86 50846 1 . G 87 87 50846 1 . G 88 88 50846 1 . C 89 89 50846 1 . U 90 90 50846 1 . G 91 91 50846 1 . G 92 92 50846 1 . C 93 93 50846 1 . U 94 94 50846 1 . C 95 95 50846 1 . U 96 96 50846 1 . G 97 97 50846 1 . G 98 98 50846 1 . G 99 99 50846 1 . C 100 100 50846 1 . U 101 101 50846 1 . U 102 102 50846 1 . U 103 103 50846 1 . C 104 104 50846 1 . C 105 105 50846 1 . U 106 106 50846 1 . A 107 107 50846 1 . U 108 108 50846 1 . G 109 109 50846 1 . U 110 110 50846 1 . A 111 111 50846 1 . U 112 112 50846 1 . G 113 113 50846 1 . C 114 114 50846 1 . U 115 115 50846 1 . A 116 116 50846 1 . U 117 117 50846 1 . G 118 118 50846 1 . C 119 119 50846 1 . A 120 120 50846 1 . U 121 121 50846 1 . A 122 122 50846 1 . C 123 123 50846 1 . U 124 124 50846 1 . A 125 125 50846 1 . C 126 126 50846 1 . C 127 127 50846 1 . U 128 128 50846 1 . G 129 129 50846 1 . C 130 130 50846 1 . C 131 131 50846 1 . U 132 132 50846 1 . G 133 133 50846 1 . G 134 134 50846 1 . U 135 135 50846 1 . G 136 136 50846 1 . G 137 137 50846 1 . U 138 138 50846 1 . G 139 139 50846 1 . C 140 140 50846 1 . U 141 141 50846 1 . C 142 142 50846 1 . C 143 143 50846 1 . U 144 144 50846 1 . A 145 145 50846 1 . A 146 146 50846 1 . U 147 147 50846 1 . A 148 148 50846 1 . A 149 149 50846 1 . A 150 150 50846 1 . C 151 151 50846 1 . A 152 152 50846 1 . U 153 153 50846 1 . G 154 154 50846 1 . C 155 155 50846 1 . U 156 156 50846 1 . A 157 157 50846 1 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 50846 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 10090 organism . 'Mus musculus' Mouse . . Eukaryota Metazoa Mus musculus . . . . . . . . . . . . . 50846 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 50846 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'enzymatic semisynthesis' . . . . . . . . . . . . . . . 'T7 in vitro transcription' 50846 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 50846 _Sample.ID 1 _Sample.Name 'Ox40 CDE' _Sample.Type solution _Sample.Sub_type . _Sample.Details 'residues 98-126' _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 "Ox40 3'UTR CDE" 'natural abundance' . . 1 $entity_1 . . 280 . . uM . . . . 50846 1 stop_ save_ save_sample_2 _Sample.Sf_category sample _Sample.Sf_framecode sample_2 _Sample.Entry_ID 50846 _Sample.ID 2 _Sample.Name 'Ox40 Terminus' _Sample.Type solution _Sample.Sub_type . _Sample.Details 'residues 1-13, uucg tetraloop, 126-140' _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 "Ox40 3'UTR Terminus" 'natural abundance' . . 1 $entity_1 . . 440 . . uM . . . . 50846 2 stop_ save_ save_sample_3 _Sample.Sf_category sample _Sample.Sf_framecode sample_3 _Sample.Entry_ID 50846 _Sample.ID 3 _Sample.Name 'Ox40 Bulge' _Sample.Type solution _Sample.Sub_type . _Sample.Details 'residues 17-51' _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 "Ox40 3'UTR Bulge" 'natural abundance' . . 1 $entity_1 . . 350 . . uM . . . . 50846 3 stop_ save_ save_sample_4 _Sample.Sf_category sample _Sample.Sf_framecode sample_4 _Sample.Entry_ID 50846 _Sample.ID 4 _Sample.Name 'Ox40 ADE' _Sample.Type solution _Sample.Sub_type . _Sample.Details 'residues 52-100' _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 "Ox40 3'UTR ADE" 'natural abundance' . . 1 $entity_1 . . 600 . . uM . . . . 50846 4 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 50846 _Sample_condition_list.ID 1 _Sample_condition_list.Name Ox40_NMRsecondarystructure _Sample_condition_list.Details 'NMR buffer: 50 mM KCl, 25 mM NaPi pH 7.0' loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 50 . mM 50846 1 pH 7.0 . pH 50846 1 pressure 1 . atm 50846 1 temperature 283 . K 50846 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 50846 _Software.ID 1 _Software.Type . _Software.Name TOPSPIN _Software.Version 4.0.6 _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID processing . 50846 1 stop_ save_ save_software_2 _Software.Sf_category software _Software.Sf_framecode software_2 _Software.Entry_ID 50846 _Software.ID 2 _Software.Type . _Software.Name NMRFAM-SPARKY _Software.Version 1.470 _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'chemical shift assignment' . 50846 2 'peak picking' . 50846 2 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 50846 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name '800 MHz' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'AVANCE III' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 800 save_ save_NMR_spectrometer_2 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_2 _NMR_spectrometer.Entry_ID 50846 _NMR_spectrometer.ID 2 _NMR_spectrometer.Name '600 MHz' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'AVANCE III' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ save_NMR_spectrometer_3 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_3 _NMR_spectrometer.Entry_ID 50846 _NMR_spectrometer.ID 3 _NMR_spectrometer.Name '700 MHz' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'AVANCE III' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 700 save_ save_NMR_spectrometer_4 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_4 _NMR_spectrometer.Entry_ID 50846 _NMR_spectrometer.ID 4 _NMR_spectrometer.Name '600 MHz 2' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'Avance II' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 50846 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '1D 1H' no . . . . . . . . . . . . 2 $sample_2 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50846 1 2 '2D 1H-1H NOESY' no . . . . . . . . . . . . 2 $sample_2 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50846 1 3 '2D 1H-15N SOFAST HMQC' no . . . . . . . . . . . . 2 $sample_2 isotropic . . 1 $sample_conditions_1 . . . 2 $NMR_spectrometer_2 . . . . . . . . . . . . . . . . . 50846 1 4 '1D 1H' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 4 $NMR_spectrometer_4 . . . . . . . . . . . . . . . . . 50846 1 5 '2D 1H-1H NOESY' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 4 $NMR_spectrometer_4 . . . . . . . . . . . . . . . . . 50846 1 6 '2D 1H-15N SOFAST HMQC' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 4 $NMR_spectrometer_4 . . . . . . . . . . . . . . . . . 50846 1 7 '1D 1H' no . . . . . . . . . . . . 3 $sample_3 isotropic . . 1 $sample_conditions_1 . . . 3 $NMR_spectrometer_3 . . . . . . . . . . . . . . . . . 50846 1 8 '2D 1H-1H NOESY' no . . . . . . . . . . . . 3 $sample_3 isotropic . . 1 $sample_conditions_1 . . . 3 $NMR_spectrometer_3 . . . . . . . . . . . . . . . . . 50846 1 9 '2D 1H-15N SOFAST HMQC' no . . . . . . . . . . . . 3 $sample_3 isotropic . . 1 $sample_conditions_1 . . . 3 $NMR_spectrometer_3 . . . . . . . . . . . . . . . . . 50846 1 10 '1D 1H' no . . . . . . . . . . . . 4 $sample_4 isotropic . . 1 $sample_conditions_1 . . . 2 $NMR_spectrometer_2 . . . . . . . . . . . . . . . . . 50846 1 11 '2D 1H-1H NOESY' no . . . . . . . . . . . . 4 $sample_4 isotropic . . 1 $sample_conditions_1 . . . 2 $NMR_spectrometer_2 . . . . . . . . . . . . . . . . . 50846 1 12 '2D 1H-15N SOFAST HMQC' no . . . . . . . . . . . . 4 $sample_4 isotropic . . 1 $sample_conditions_1 . . . 2 $NMR_spectrometer_2 . . . . . . . . . . . . . . . . . 50846 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 50846 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name Referencing_Ox40_UTRassignment _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID H 1 DSS 'methyl protons' . . . . ppm 0 internal direct 1 . . . . . 50846 1 N 15 DSS 'methyl protons' . . . . ppm 0 internal indirect 0.1013 . . . . . 50846 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_1 _Assigned_chem_shift_list.Entry_ID 50846 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name Chemicalshifts_Ox40mRNA3UTR _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 2 '2D 1H-1H NOESY' . . . 50846 1 3 '2D 1H-15N SOFAST HMQC' . . . 50846 1 5 '2D 1H-1H NOESY' . . . 50846 1 6 '2D 1H-15N SOFAST HMQC' . . . 50846 1 8 '2D 1H-1H NOESY' . . . 50846 1 9 '2D 1H-15N SOFAST HMQC' . . . 50846 1 11 '2D 1H-1H NOESY' . . . 50846 1 12 '2D 1H-15N SOFAST HMQC' . . . 50846 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 2 $software_2 . . 50846 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 1 1 G H1 H 1 13.143 0.003 . . . . . . . 1 G H1 . 50846 1 2 . 1 . 1 1 1 G N1 N 15 147.832 0.000 . . . . . . . 1 G N1 . 50846 1 3 . 1 . 1 4 4 U H3 H 1 11.792 0.001 . . . . . . . 4 U H3 . 50846 1 4 . 1 . 1 4 4 U N3 N 15 157.447 0.000 . . . . . . . 4 U N3 . 50846 1 5 . 1 . 1 5 5 U H3 H 1 12.006 0.003 . . . . . . . 5 U H3 . 50846 1 6 . 1 . 1 5 5 U N3 N 15 157.602 0.000 . . . . . . . 5 U N3 . 50846 1 7 . 1 . 1 8 8 U H3 H 1 11.477 0.000 . . . . . . . 8 U H3 . 50846 1 8 . 1 . 1 8 8 U N3 N 15 157.615 0.000 . . . . . . . 8 U N3 . 50846 1 9 . 1 . 1 12 12 G H1 H 1 12.825 0.003 . . . . . . . 12 G H1 . 50846 1 10 . 1 . 1 12 12 G N1 N 15 147.006 0.000 . . . . . . . 12 G N1 . 50846 1 11 . 1 . 1 13 13 G H1 H 1 13.740 0.007 . . . . . . . 13 G H1 . 50846 1 12 . 1 . 1 13 13 G N1 N 15 148.324 0.000 . . . . . . . 13 G N1 . 50846 1 13 . 1 . 1 26 26 G H1 H 1 11.968 0.001 . . . . . . . 26 G H1 . 50846 1 14 . 1 . 1 26 26 G N1 N 15 146.332 0.000 . . . . . . . 26 G N1 . 50846 1 15 . 1 . 1 27 27 G H1 H 1 12.793 0.002 . . . . . . . 27 G H1 . 50846 1 16 . 1 . 1 27 27 G N1 N 15 147.200 0.000 . . . . . . . 27 G N1 . 50846 1 17 . 1 . 1 28 28 G H1 H 1 12.982 0.010 . . . . . . . 28 G H1 . 50846 1 18 . 1 . 1 28 28 G N1 N 15 148.257 0.000 . . . . . . . 28 G N1 . 50846 1 19 . 1 . 1 52 52 G H1 H 1 13.404 0.004 . . . . . . . 52 G H1 . 50846 1 20 . 1 . 1 52 52 G N1 N 15 148.084 0.000 . . . . . . . 52 G N1 . 50846 1 21 . 1 . 1 55 55 U H3 H 1 11.349 0.003 . . . . . . . 55 U H3 . 50846 1 22 . 1 . 1 56 56 G H1 H 1 13.198 0.002 . . . . . . . 56 G H1 . 50846 1 23 . 1 . 1 56 56 G N1 N 15 148.422 0.000 . . . . . . . 56 G N1 . 50846 1 24 . 1 . 1 60 60 G H1 H 1 13.319 0.000 . . . . . . . 60 G H1 . 50846 1 25 . 1 . 1 60 60 G N1 N 15 148.248 0.000 . . . . . . . 60 G N1 . 50846 1 26 . 1 . 1 80 80 G H1 H 1 12.533 0.002 . . . . . . . 80 G H1 . 50846 1 27 . 1 . 1 80 80 G N1 N 15 146.942 0.000 . . . . . . . 80 G N1 . 50846 1 28 . 1 . 1 81 81 G H1 H 1 13.147 0.003 . . . . . . . 81 G H1 . 50846 1 29 . 1 . 1 81 81 G N1 N 15 147.956 0.000 . . . . . . . 81 G N1 . 50846 1 30 . 1 . 1 82 82 U H3 H 1 14.160 0.002 . . . . . . . 82 U H3 . 50846 1 31 . 1 . 1 90 90 U H3 H 1 13.760 0.002 . . . . . . . 90 U H3 . 50846 1 32 . 1 . 1 90 90 U N3 N 15 162.385 0.000 . . . . . . . 90 U N3 . 50846 1 33 . 1 . 1 91 91 G H1 H 1 11.956 0.001 . . . . . . . 91 G H1 . 50846 1 34 . 1 . 1 91 91 G N1 N 15 146.216 0.000 . . . . . . . 91 G N1 . 50846 1 35 . 1 . 1 92 92 G H1 H 1 13.305 0.002 . . . . . . . 92 G H1 . 50846 1 36 . 1 . 1 92 92 G N1 N 15 148.243 0.000 . . . . . . . 92 G N1 . 50846 1 37 . 1 . 1 98 98 G H1 H 1 12.626 0.004 . . . . . . . 98 G H1 . 50846 1 38 . 1 . 1 98 98 G N1 N 15 147.405 0.000 . . . . . . . 98 G N1 . 50846 1 39 . 1 . 1 99 99 G H1 H 1 12.710 0.006 . . . . . . . 99 G H1 . 50846 1 40 . 1 . 1 99 99 G N1 N 15 147.450 0.000 . . . . . . . 99 G N1 . 50846 1 41 . 1 . 1 109 109 G H1 H 1 13.175 0.005 . . . . . . . 109 G H1 . 50846 1 42 . 1 . 1 109 109 G N1 N 15 147.887 0.000 . . . . . . . 109 G N1 . 50846 1 43 . 1 . 1 110 110 U H3 H 1 13.388 0.007 . . . . . . . 110 U H3 . 50846 1 44 . 1 . 1 110 110 U N3 N 15 161.641 0.000 . . . . . . . 110 U N3 . 50846 1 45 . 1 . 1 112 112 U H3 H 1 13.540 0.003 . . . . . . . 112 U H3 . 50846 1 46 . 1 . 1 112 112 U N3 N 15 161.912 0.000 . . . . . . . 112 U N3 . 50846 1 47 . 1 . 1 113 113 G H1 H 1 12.519 0.005 . . . . . . . 113 G H1 . 50846 1 48 . 1 . 1 113 113 G N1 N 15 146.967 0.000 . . . . . . . 113 G N1 . 50846 1 49 . 1 . 1 118 118 G H1 H 1 12.929 0.004 . . . . . . . 118 G H1 . 50846 1 50 . 1 . 1 118 118 G N1 N 15 148.476 0.000 . . . . . . . 118 G N1 . 50846 1 51 . 1 . 1 121 121 U H3 H 1 13.313 0.004 . . . . . . . 121 U H3 . 50846 1 52 . 1 . 1 121 121 U N3 N 15 161.340 0.000 . . . . . . . 121 U N3 . 50846 1 53 . 1 . 1 128 128 U H3 H 1 13.751 0.007 . . . . . . . 128 U H3 . 50846 1 54 . 1 . 1 128 128 U N3 N 15 161.944 0.000 . . . . . . . 128 U N3 . 50846 1 55 . 1 . 1 129 129 G H1 H 1 12.413 0.002 . . . . . . . 129 G H1 . 50846 1 56 . 1 . 1 129 129 G N1 N 15 147.415 0.000 . . . . . . . 129 G N1 . 50846 1 57 . 1 . 1 134 134 G H1 H 1 13.226 0.003 . . . . . . . 134 G H1 . 50846 1 58 . 1 . 1 134 134 G N1 N 15 147.786 0.000 . . . . . . . 134 G N1 . 50846 1 59 . 1 . 1 135 135 U H3 H 1 13.718 0.011 . . . . . . . 135 U H3 . 50846 1 60 . 1 . 1 135 135 U N3 N 15 162.312 0.000 . . . . . . . 135 U N3 . 50846 1 61 . 1 . 1 136 136 G H1 H 1 10.117 0.003 . . . . . . . 136 G H1 . 50846 1 62 . 1 . 1 136 136 G N1 N 15 142.999 0.000 . . . . . . . 136 G N1 . 50846 1 63 . 1 . 1 137 137 G H1 H 1 11.764 0.010 . . . . . . . 137 G H1 . 50846 1 64 . 1 . 1 137 137 G N1 N 15 144.633 0.000 . . . . . . . 137 G N1 . 50846 1 65 . 1 . 1 138 138 U H3 H 1 13.772 0.004 . . . . . . . 138 U H3 . 50846 1 66 . 1 . 1 138 138 U N3 N 15 162.371 0.000 . . . . . . . 138 U N3 . 50846 1 67 . 1 . 1 139 139 G H1 H 1 12.632 0.002 . . . . . . . 139 G H1 . 50846 1 68 . 1 . 1 139 139 G N1 N 15 147.112 0.000 . . . . . . . 139 G N1 . 50846 1 stop_ save_