# 1 "/u/exp/stan/nmr/lists/pp/invietf3gpsi" # 1 "/u/exp/stan/nmr/lists/pp/invietf3gpsi" ;invif3etgpsi ;avance-version (00/04/28) ;HSQC ;2D H-1/X correlation via double inept transfer ; using sensitivity improvement ;phase sensitive using Echo/Antiecho-TPPI gradient selection ;with decoupling during acquisition ;using trim pulses in inept transfer ;using f3 - channel ;A.G. Palmer III, J. Cavanagh, P.E. Wright & M. Rance, J. Magn. ; Reson. 93, 151-170 (1991) ;L.E. Kay, P. Keifer & T. Saarinen, J. Am. Chem. Soc. 114, ; 10663-5 (1992) ;J. Schleucher, M. Schwendinger, M. Sattler, P. Schmidt, O. Schedletzky, ; S.J. Glaser, O.W. Sorensen & C. Griesinger, J. Biomol. NMR 4, ; 301-306 (1994) # 1 "/u/exp/stan/nmr/lists/pp/Avance.incl" 1 ;Avance.incl ; ;version 00/07/27 ;switch between high and low stage oh H amplifier ;use 2H channel for lock or pulse (lockswitch) ;allow for 2H decoupling (lockswitch) ;turn lock-hold on/off (BSMS) ;switch between 1H or 19F output (H amplifier) ;select output for 19F (amplifier) ;homospoil on/off (BSMS) ;for Q-switch probes ;for mixing probe ;gating pulse for RX22, ADC and HPPR ;not active ;$Id: pulseprogram,v 1.1 2021/02/10 22:31:52 bmrbsvc Exp $ # 20 "/u/exp/stan/nmr/lists/pp/invietf3gpsi" 2 # 1 "/u/exp/stan/nmr/lists/pp/Grad.incl" 1 ;Grad.incl - include file for Gradient Spectroscopy ; ;avance-version (00/07/27) ;gradient pulse for gs-syntax ;blank/unblank gradient amplifier and turn lock-hold on/off ;blank/unblank gradient amplifier ;for RCB board (BSMS) define list EA= ;$Id: pulseprogram,v 1.1 2021/02/10 22:31:52 bmrbsvc Exp $ # 21 "/u/exp/stan/nmr/lists/pp/invietf3gpsi" 2 # 1 "/u/exp/stan/nmr/lists/pp/Delay.incl" 1 ;Delay.incl - include file for commonly used delays ; ;version 00/02/07 ;general delays define delay DELTA define delay DELTA1 define delay DELTA2 define delay DELTA3 define delay DELTA4 define delay DELTA5 define delay DELTA6 define delay DELTA7 define delay DELTA8 define delay TAU define delay TAU1 define delay TAU2 define delay TAU3 define delay TAU4 define delay TAU5 ;delays for centering pulses define delay CEN_HN1 define delay CEN_HN2 define delay CEN_HN3 define delay CEN_HC1 define delay CEN_HC2 define delay CEN_HC3 define delay CEN_HC4 define delay CEN_HP1 define delay CEN_HP2 define delay CEN_CN1 define delay CEN_CN2 define delay CEN_CN3 define delay CEN_CN4 define delay CEN_CP1 define delay CEN_CP2 ;loop counters define loopcounter COUNTER define loopcounter SCALEF define loopcounter FACTOR1 define loopcounter FACTOR2 define loopcounter FACTOR3 ;$Id: pulseprogram,v 1.1 2021/02/10 22:31:52 bmrbsvc Exp $ # 22 "/u/exp/stan/nmr/lists/pp/invietf3gpsi" 2 "p2=p1*2" "p22=p21*2" "d0=3u" "d11=30m" "d13=4u" "d26=1s/(cnst4*4)" "DELTA=p16+d16+p2+d0*2" "DELTA1=d13+p16+d16+4u" "CEN_HN1=(p21-p1)/2" "CEN_HN2=(p22-p2)/2" # 1 "mc_line 46 file /u/exp/stan/nmr/lists/pp/invietf3gpsi expanding definition part of mc command before ze" ; dimension 2 aq-mode (F2) undefined (F1) Echo-Antiecho define delay MCWRK define delay MCREST define loopcounter ST1CNT "ST1CNT = td1 / ( 2 ) " "MCWRK = 0.166667 * d1" "MCREST = d1 - d1" # 46 "/u/exp/stan/nmr/lists/pp/invietf3gpsi" 1 ze d11 pl16:f3 # 1 "mc_line 48 file /u/exp/stan/nmr/lists/pp/invietf3gpsi expanding start label for mc command" 2 MCWRK*2 do:f3 LBLSTS1, MCWRK*4 LBLF1, MCREST # 49 "/u/exp/stan/nmr/lists/pp/invietf3gpsi" 3 (p1 ph1) d26 pl3:f3 (CEN_HN2 p2 ph1) (p22 ph6):f3 d26 setnmr2|0 setnmr0|34|32|33 p28 ph1 d13 (p1 ph2) 3u p16:gp1 d16 (p21 ph3):f3 d0 p2 ph7 d0 p16:gp2*EA d16 (p22 ph4):f3 DELTA (CEN_HN1 p1 ph1) (p21 ph4):f3 d24 (CEN_HN2 p2 ph1) (p22 ph1):f3 d24 (CEN_HN1 p1 ph2) (p21 ph5):f3 d26 (CEN_HN2 p2 ph1) (p22 ph1):f3 d26 (p1 ph1) DELTA1 (p2 ph1) d13 p16:gp3 d16 pl16:f3 4u setnmr2^0 setnmr0^34^32^33 go=2 ph31 cpd3:f3 # 1 "mc_line 83 file /u/exp/stan/nmr/lists/pp/invietf3gpsi expanding mc command in line " MCWRK do:f3 wr #0 if #0 zd igrad EA MCWRK ip5*2 lo to LBLSTS1 times 2 MCWRK id0 MCWRK ip3*2 MCWRK ip6*2 MCWRK ip31*2 lo to LBLF1 times ST1CNT # 84 "/u/exp/stan/nmr/lists/pp/invietf3gpsi" exit ph1=0 ph2=1 ph3=0 2 ph4=0 0 2 2 ph5=1 1 3 3 ph6=0 ph7=0 0 2 2 ph31=0 2 2 0 ;pl1 : f1 channel - power level for pulse (default) ;pl3 : f3 channel - power level for pulse (default) ;pl16: f3 channel - power level for CPD/BB decoupling ;p1 : f1 channel - 90 degree high power pulse ;p2 : f1 channel - 180 degree high power pulse ;p16: homospoil/gradient pulse [1 msec] ;p21: f3 channel - 90 degree high power pulse ;p22: f3 channel - 180 degree high power pulse ;p28: f1 channel - trim pulse [1 msec] ;d0 : incremented delay (2D) [3 usec] ;d1 : relaxation delay; 1-5 * T1 ;d11: delay for disk I/O [30 msec] ;d13: short delay [4 usec] ;d16: delay for homospoil/gradient recovery ;d24: 1/(4J)YH for YH ; 1/(8J)YH for all multiplicities ;d26: 1/(4J(YH)) ;cnst4: = J(YH) ;in0: 1/(2 * SW(X)) = DW(X) ;nd0: 2 ;NS: 1 * n ;DS: >= 16 ;td1: number of experiments ;FnMODE: echo-antiecho ;cpd3: decoupling according to sequence defined by cpdprg3 ;pcpd3: f3 channel - 90 degree pulse for decoupling sequence ;use gradient ratio: gp 1 : gp 2 : gp 3 ; 50 : 80 : 20.1 for C-13 ; 50 : 80 : 8.1 for N-15 ;for z-only gradients: ;gpz1: 50% ;gpz2: 80% ;gpz3: 20.1% for C-13, 8.1% for N-15 ;use gradient files: ;gpnam1: SINE.100 ;gpnam2: SINE.100 ;gpnam3: SINE.100 ;$Id: pulseprogram,v 1.1 2021/02/10 22:31:52 bmrbsvc Exp $