# 1 "/u/exp/stan/nmr/lists/pp/hccconhgpwg3d3.fp" ;hccconhgpwg3d3 ;avance-version (03/02/26) ;HCCCONH ;3D sequence with ; inverse correlation for triple resonance using ; dipsi2 and inept transfer steps ; ; F1(H) -> F2(Caliph.,t1 -> Ca) -> F2(C=O) -> F3(N,t2) -> F1(H,t3) ; ;on/off resonance Ca and C=O pulses using shaped pulse ;phase sensitive (t1) ;phase sensitive (t2) ;using constant time in t2 ;water suppression using watergate sequence ;(use parameterset HCCCONHGPWG3D3) ; ;G.T. Montelione, B.A. Lyons, S.D. Emerson & M. Tashiro, ; J. Am. Chem. Soc. 114, 10974-75 (1992) ;S. Grzesiek, J. Anglister & A. Bax, J. Magn. Reson. 101 B, 114-9 (1993) ;B.A. Lyons & G.T. Montelione, J. Magn. Reson. 101 B, 206-9 (1993) ;T.M. Logan, E.T. Olejniczak, R.X. Xu & S.W. Fesik, ; J. Biomol. NMR 3, 225-31 (1993) ;R.T. Clowes, W. Boucher, C.H. Hardman, P.J. Domaille & E.D. Laue, ; J. Biomol. NMR 3, 349-354 (1993) ;T. Carlomagno, M. Maurer, M. Sattler, M.G. Schwendinger, S.J. Glaser ; & C. Griesinger, J. Biomol. NMR 8, 161-170 (1996) prosol relations= # 1 "/u/exp/stan/nmr/lists/pp/Avance.incl" 1 ;Avance.incl ; ;avance-version (03/02/17) ;$Id: pulseprogram,v 1.1 2021/02/11 14:21:20 bmrbsvc Exp $ # 32 "/u/exp/stan/nmr/lists/pp/hccconhgpwg3d3.fp" 2 # 1 "/u/exp/stan/nmr/lists/pp/Grad.incl" 1 ;Grad.incl - include file for Gradient Spectroscopy ; ;avance-version (02/05/31) define list EA= ;$Id: pulseprogram,v 1.1 2021/02/11 14:21:20 bmrbsvc Exp $ # 33 "/u/exp/stan/nmr/lists/pp/hccconhgpwg3d3.fp" 2 # 1 "/u/exp/stan/nmr/lists/pp/Delay.incl" 1 ;Delay.incl - include file for commonly used delays ; ;version 00/02/07 ;general delays define delay DELTA define delay DELTA1 define delay DELTA2 define delay DELTA3 define delay DELTA4 define delay DELTA5 define delay DELTA6 define delay DELTA7 define delay DELTA8 define delay TAU define delay TAU1 define delay TAU2 define delay TAU3 define delay TAU4 define delay TAU5 ;delays for centering pulses define delay CEN_HN1 define delay CEN_HN2 define delay CEN_HN3 define delay CEN_HC1 define delay CEN_HC2 define delay CEN_HC3 define delay CEN_HC4 define delay CEN_HP1 define delay CEN_HP2 define delay CEN_CN1 define delay CEN_CN2 define delay CEN_CN3 define delay CEN_CN4 define delay CEN_CP1 define delay CEN_CP2 ;loop counters define loopcounter COUNTER define loopcounter SCALEF define loopcounter FACTOR1 define loopcounter FACTOR2 define loopcounter FACTOR3 ;$Id: pulseprogram,v 1.1 2021/02/11 14:21:20 bmrbsvc Exp $ # 34 "/u/exp/stan/nmr/lists/pp/hccconhgpwg3d3.fp" 2 "d0=3u" "d10=3u" "d11=30m" "d12=20u" "d13=4u" "d14=p14" "d3=1.1m" "d4=1.7m" "d21=3.6m" "d22=4.4m" "d23=12.4m" "d25=5.5m" "d26=2.3m" "in30=in10" "d30=d23" "l1=(d15/(p9*115.112))+0.5" "CEN_CN2=(p14-p22)/2" "DELTA1=d3+p2+d0*2+larger(p14,p22)-p14-4u" "DELTA2=d23+4u-d22" "DELTA3=d23-d10-p14" "DELTA4=d26-p16-d16-p11-12u" "TAU1=d23*2+p14-d25-p26-d13" "spoff2=0" "spoff3=0" "spoff5=bf2*((cnst21-cnst23)/1000000)" "spoff7=bf2*((cnst22-cnst21)/1000000)" "spoff8=0" "spoff9=0" aqseq 321 # 1 "mc_line 85 file /u/exp/stan/nmr/lists/pp/hccconhgpwg3d3.fp expanding definition part of mc command before ze" ; dimension 3 aq-mode (F2) States-TPPI (F1) States-TPPI F2->F1 define delay MCWRK define delay MCREST define loopcounter ST1CNT "ST1CNT = td1 / (2)" define loopcounter ST2CNT "ST2CNT = td2 / (2)" "MCWRK = 0.142857*d11" "MCREST = d11 - d11" # 85 "/u/exp/stan/nmr/lists/pp/hccconhgpwg3d3.fp" 1 ze # 1 "mc_line 85 file /u/exp/stan/nmr/lists/pp/hccconhgpwg3d3.fp expanding definition of mc command after ze" # 86 "/u/exp/stan/nmr/lists/pp/hccconhgpwg3d3.fp" d11 pl16:f3 # 1 "mc_line 87 file /u/exp/stan/nmr/lists/pp/hccconhgpwg3d3.fp expanding start label for mc command" 2 MCWRK do:f3 LBLSTS2, MCWRK * 2 LBLF2, MCWRK * 3 LBLSTS1, MCWRK LBLF1, MCREST # 88 "/u/exp/stan/nmr/lists/pp/hccconhgpwg3d3.fp" 3 d11 fq=cnst23(bf ppm):f2 d1 50u setnmr2|0 setnmr0|34|32|33 d12 pl1:f1 pl0:f2 pl3:f3 (p1 ph3):f1 d4 (center (p2 ph1):f1 (p14:sp3 ph1):f2 ) d4 (p1 ph2):f1 4u p16:gp1 d16 (p13:sp2 ph4):f2 d0 (center (p14:sp5 ph1):f2 (p22 ph1):f3 ) d3 (p2 ph1):f1 d0 (p14:sp3 ph1):f2 DELTA1 (p14:sp5 ph1):f2 4u (p13:sp8 ph2):f2 4u 4u pl15:f2 ;begin DIPSI2 7 (p9*3.556 ph23):f2 (p9*4.556 ph25):f2 (p9*3.222 ph23):f2 (p9*3.167 ph25):f2 (p9*0.333 ph23):f2 (p9*2.722 ph25):f2 (p9*4.167 ph23):f2 (p9*2.944 ph25):f2 (p9*4.111 ph23):f2 (p9*3.556 ph25):f2 (p9*4.556 ph23):f2 (p9*3.222 ph25):f2 (p9*3.167 ph23):f2 (p9*0.333 ph25):f2 (p9*2.722 ph23):f2 (p9*4.167 ph25):f2 (p9*2.944 ph23):f2 (p9*4.111 ph25):f2 (p9*3.556 ph25):f2 (p9*4.556 ph23):f2 (p9*3.222 ph25):f2 (p9*3.167 ph23):f2 (p9*0.333 ph25):f2 (p9*2.722 ph23):f2 (p9*4.167 ph25):f2 (p9*2.944 ph23):f2 (p9*4.111 ph25):f2 (p9*3.556 ph23):f2 (p9*4.556 ph25):f2 (p9*3.222 ph23):f2 (p9*3.167 ph25):f2 (p9*0.333 ph23):f2 (p9*2.722 ph25):f2 (p9*4.167 ph23):f2 (p9*2.944 ph25):f2 (p9*4.111 ph23):f2 lo to 7 times l1 ;end DIPSI2 d12 pl19:f1 pl0:f2 (p26 ph1):f1 d12 cpds1:f1 ph2 (p13:sp2 ph1):f2 4u (p14:sp5 ph1):f2 d21 (p24:sp9 ph1):f2 4u (p14:sp5 ph1):f2 d21 (p13:sp8 ph2):f2 4u d12 fq=cnst21(bf ppm):f2 (p13:sp2 ph5):f2 d22 (p14:sp7 ph1):f2 DELTA2 pl3:f3 (center (p14:sp3 ph1):f2 (p22 ph8):f3 ) d23 (p14:sp7 ph1):f2 4u (p13:sp8 ph1):f2 4u 4u do:f1 (p26 ph10):f1 4u p16:gp2 d16 (p26 ph2):f1 4u cpds1:f1 ph1 (p21 ph6):f3 (TAU1 d13 do p26 ph9):f1 (d10 p14:sp7 ph1 DELTA3 p14:sp3 ph1 d30):f2 (d10 d14 DELTA3 CEN_CN2 p22 ph1):f3 ; d10 ; (p14:sp7 ph1):f2 ; DELTA3 ; (center (p14:sp3 ph1):f2 (p22 ph1):f3 ) ; d30 ; d13 do:f1 ; (p26 ph9):f1 ; d25 (p21 ph1):f3 p16:gp3 d16 pl0:f1 (p11:sp1 ph7):f1 4u 4u pl1:f1 (p1 ph1) 4u p16:gp4 d16 DELTA4 pl0:f1 (p11:sp1 ph7):f1 4u 4u pl1:f1 (center (p2 ph1) (p22 ph1):f3 ) 4u pl0:f1 (p11:sp1 ph7):f1 4u DELTA4 p16:gp4 d16 pl16:f3 4u setnmr2^0 setnmr0^34^32^33 go=2 ph31 cpd3:f3 # 1 "mc_line 234 file /u/exp/stan/nmr/lists/pp/hccconhgpwg3d3.fp expanding mc command in line" MCWRK do:f3 wr #0 if #0 zd ip6 lo to LBLSTS2 times 2 MCWRK id10 MCWRK dd30 lo to LBLF2 times ST2CNT MCWRK rd10 MCWRK rd30 MCWRK ip4 lo to LBLSTS1 times 2 MCWRK id0 lo to LBLF1 times ST1CNT # 237 "/u/exp/stan/nmr/lists/pp/hccconhgpwg3d3.fp" exit ph1=0 ph2=1 ph3=0 0 0 0 0 0 0 0 2 2 2 2 2 2 2 2 ph4=0 0 2 2 ph5=0 0 0 0 2 2 2 2 ph6=0 2 ph7=2 ph8=0 ph9=3 ph10=2 ph23=0 ph25=2 ph31=0 2 2 0 2 0 0 2 2 0 0 2 0 2 2 0 ;pl0 : 120dB ;pl1 : f1 channel - power level for pulse (default) ;pl3 : f3 channel - power level for pulse (default) ;pl15: f2 channel - power level for TOCSY-spinlock ;pl16: f3 channel - power level for CPD/BB decoupling ;pl19: f1 channel - power level for CPD/BB decoupling ;sp1: f1 channel - shaped pulse 90 degree (H2O on resonance) ;sp2: f2 channel - shaped pulse 90 degree (C=O on resonance) ;sp3: f2 channel - shaped pulse 180 degree (C=O on resonance) ;sp5: f2 channel - shaped pulse 180 degree (C=O off resonance) ;sp7: f2 channel - shaped pulse 180 degree (Ca off resonance) ;sp8: f2 channel - shaped pulse 90 degree (on resonance) ; for time reversed pulse ;sp9: f2 channel - shaped pulse 180 degree (Ca on resonance) ; sp9 might require higher selectivity than sp3 ;p1 : f1 channel - 90 degree high power pulse ;p2 : f1 channel - 180 degree high power pulse ;p9 : f2 channel - 90 degree low power pulse ;p11: f1 channel - 90 degree shaped pulse [1 msec] ;p13: f2 channel - 90 degree shaped pulse ;p14: f2 channel - 180 degree shaped pulse ;p16: homospoil/gradient pulse [1 msec] ;p21: f3 channel - 90 degree high power pulse ;p22: f3 channel - 180 degree high power pulse ;p24: f2 channel - 180 degree shaped pulse (sp9) ;p26: f1 channel - 90 degree pulse at pl19 ;d0 : incremented delay (F1 in 3D) [3 usec] ;d1 : relaxation delay; 1-5 * T1 ;d3 : 1/(6J(CH) [1.1 msec] ;d4: 1/(4J(CH) [1.7 msec] ;d10: incremented delay (F2 in 3D) [3 usec] ;d11: delay for disk I/O [30 msec] ;d12: delay for power switching [20 usec] ;d13: short delay [4 usec] ;d15: TOCSY mixing time [12 msec] ;d16: delay for homospoil/gradient recovery ;d21: 1/(2J(CaCO)) [3.6 msec] ;d22: 1/(2J'(CaCO) [4.4 msec] ;d23: constant time delay T(N) = 1/(4J'(NCO) [12.4 msec] ;d25: 1/(2J'(NH)) [5.5 msec] ;d26: 1/(4J(NH)) [2.3 msec] ;d30: decremented delay (F2 in 3D) = d23-d25-d13-p26 ;cnst21: CO chemical shift (offset, in ppm) ;cnst22: Calpha chemical shift (offset, in ppm) ;cnst23: Caliphatic chemical shift (offset, in ppm) ;o2p: Caliphatic chemical shift (cnst23) ;l1: loop for DIPSI2 cycle: ((p6*115.112) * l1) = mixing time ;in0: 1/(2 * SW(Cali)) = DW(Cali) ;nd0: 2 ;in10: 1/(2 * SW(N)) = DW(N) ;nd10: 2 ;in30: = in10 ;NS: 16 * n ;DS: >= 16 ;td1: number of experiments in F1 ;td2: number of experiments in F2 td2 max = 2 * d30 / in30 ;FnMODE: States-TPPI (or TPPI) in F1 ;FnMODE: States-TPPI (or TPPI) in F2 ;cpds1: decoupling according to sequence defined by cpdprg1 ;cpd3: decoupling according to sequence defined by cpdprg3 ;pcpd1: f1 channel - 90 degree pulse for decoupling sequence ;pcpd3: f3 channel - 90 degree pulse for decoupling sequence ;use gradient ratio: gp 1 : gp 2 : gp 3 : gp 4 ; 50 : 40 : 60 : 30 ;for z-only gradients: ;gpz1: 50% ;gpz2: 40% ;gpz3: 60% ;gpz4: 30% ;use gradient files: ;gpnam1: SINE.100 ;gpnam2: SINE.100 ;gpnam3: SINE.100 ;gpnam4: SINE.100 ;$Id: pulseprogram,v 1.1 2021/02/11 14:21:20 bmrbsvc Exp $