********  PROTEIN ********

Name:  			ZNF42	
Organism: 		Homo sapiens
Gene:			ZNF42	
CESG ID:		GO.79132
Expression system:      Wheat germ cell-free system
PDB code:		2fi2
BMRB code:		69.57
Sample conditions:	

Sample #1:		2.0 mM [U-15N/13C]ZNF42
			50 mM NaCl
			20 mM sodium phosphate
			pH 7.4, 303 K

Sample #2:              1.7 mM 50% [U-15N/13C]ZNF42: 50% unlabeled ZNF42
                        50 mM NaCl
                        20 mM sodium phosphate
                        pH 7.4, 303 K

******** NMR EXPERIMENTS ********

Spectrometer:		Bruker DRX 600 MHz

HSQC:			15N HSQC, 13C aromatic HSQC
Triple resonance:	HNCO, HNCOCA, HNCA, HNCACB, HNCACO, CCONH, HCCH-TOCSY, HCCONH
NOESY:			3D 15N-NOESY, 3D 13C-NOESY aliphatic, 3D 13C-NOESY aromatic, 
			3D F1-13C-filtered F3-13C-edited NOESY (used sample #2)

--Each directory contains raw data, pulse sequences, acquisition parameters, and processing scripts

******** SOFTWARE ********

Collection:		XWINNMR
Processing:		NMRPipe
Analysis:		XEASY
Peak picking:		SPSCAN and XEASY
Backbone assignments:	GARANT
Structure calculation:	CYANA (CANDID used for automated NOE assignments)
Water refinement:	XPLOR-NIH

******** CONTACT ********

Brian F. Volkman:	bvolkman@biochem.mcw.edu