BMRB Entry 6497
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6497
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Title: Magnetic Field Dependence of Nitrogen-Proton J Splittings in 15N-Enriched Human Ubiquitin Resulting from Relaxation Interference and Residual Dipolar Coupling
Deposition date: 2005-02-09 Original release date: 2021-04-29
Authors: Tjandra, Nico; Grzesiek, Stephan; Bax, Ad
Citation: Tjandra, Nico; Grzesiek, Stephan; Bax, Ad. "Magnetic Field Dependence of Nitrogen-Proton J Splittings in 15N-Enriched Human Ubiquitin Resulting from Relaxation Interference and Residual Dipolar Coupling" J. Am. Chem. Soc. 118, 6264-6272 (1996).
Assembly members:
Ubiquitin, polymer, 76 residues, 8579.94 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
Ubiquitin: MQIFVKTLTGKTITLEVEPS
DTIENVKAKIQDKEGIPPDQ
QRLIFAGKQLEDGRTLSDYN
IQKESTLHLVLRLRGG
| Data type | Count |
| coupling constants | 395 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ubiquitin | 1 |
Entities:
Entity 1, Ubiquitin 76 residues - 8579.94 Da.
| 1 | MET | GLN | ILE | PHE | VAL | LYS | THR | LEU | THR | GLY | ||||
| 2 | LYS | THR | ILE | THR | LEU | GLU | VAL | GLU | PRO | SER | ||||
| 3 | ASP | THR | ILE | GLU | ASN | VAL | LYS | ALA | LYS | ILE | ||||
| 4 | GLN | ASP | LYS | GLU | GLY | ILE | PRO | PRO | ASP | GLN | ||||
| 5 | GLN | ARG | LEU | ILE | PHE | ALA | GLY | LYS | GLN | LEU | ||||
| 6 | GLU | ASP | GLY | ARG | THR | LEU | SER | ASP | TYR | ASN | ||||
| 7 | ILE | GLN | LYS | GLU | SER | THR | LEU | HIS | LEU | VAL | ||||
| 8 | LEU | ARG | LEU | ARG | GLY | GLY |
Samples:
sample_1: Ubiquitin, [U-15N], 1.4 mM; NaCl 10 mM
conditions_1: pH: 4.7; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H15N HSQC | not available | not available | not available |
| 1JNH-modulated 2D HSQC | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker AMX 360 MHz
- Bruker DMX 500 MHz
- Bruker AMX 600 MHz
- Bruker DMX 750 MHz