BMRB Entry 16162
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16162
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Title: Beta1D Integrin Cytoplasmic Tail (Polyhistidine-Tagged) 1H and 15N Chemical Shift Assignments PubMed: 19798053
Deposition date: 2009-02-07 Original release date: 2009-10-07
Authors: Anthis, Nicholas; Wegener, Kate; Campbell, Iain
Citation: Anthis, Nicholas; Wegener, Kate; Ye, Feng; Kim, Chungho; Goult, Benjamin; Lowe, Edward; Vakonakis, Ioannis; Bate, Neil; Critchley, David; Ginsberg, Mark; Campbell, Iain. "The structure of an integrin/talin complex reveals the basis of inside-out signal transduction." EMBO J. 28, 3623-3632 (2009).
Assembly members:
beta1D_integrin_with_C-terminal_tag, polymer, 60 residues, 7251 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET30b
Entity Sequences (FASTA):
beta1D_integrin_with_C-terminal_tag: MKLLMIIHDRREFAKFEKEK
MNAKWDTQENPIYKSPINNF
KNPNYGRKAGLGSEHHHHHH
- assigned_chemical_shifts
Data type | Count |
15N chemical shifts | 118 |
1H chemical shifts | 392 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | beta1D integrin tail | 1 |
Entities:
Entity 1, beta1D integrin tail 60 residues - 7251 Da.
This is the cytoplasmic tail of the beta1D integrin tail. The first residue (M) and the last nine residues (GSEHHHHHH) are cloning artifacts.
1 | MET | LYS | LEU | LEU | MET | ILE | ILE | HIS | ASP | ARG | |
2 | ARG | GLU | PHE | ALA | LYS | PHE | GLU | LYS | GLU | LYS | |
3 | MET | ASN | ALA | LYS | TRP | ASP | THR | GLN | GLU | ASN | |
4 | PRO | ILE | TYR | LYS | SER | PRO | ILE | ASN | ASN | PHE | |
5 | LYS | ASN | PRO | ASN | TYR | GLY | ARG | LYS | ALA | GLY | |
6 | LEU | GLY | SER | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
pH_6.1: D2O 5%; sodium phosphate 50 mM; sodium chloride 100 mM; DTT 1 mM; DSS 0.1 mM; beta1D integrin with C-terminal tag, [U-15N], 0.05 mM; H2O 95%
pH_5.0: D2O 5%; sodium acetate 20 mM; sodium azide 0.02%; DSS 0.1 mM; beta1D integrin with C-terminal tag, [U-15N], 1 mM; D2O 5%
pH_6.1_conditions: ionic strength: 200 mM; pH: 6.1; pressure: 1 atm; temperature: 298 K
pH_5.0_conditions: ionic strength: 10 mM; pH: 5.0; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | pH_6.1 | isotropic | pH_6.1_conditions |
3D 1H-15N NOESY | pH_5.0 | isotropic | pH_5.0_conditions |
3D 1H-15N TOCSY | pH_5.0 | isotropic | pH_5.0_conditions |
2D 1H-15N HSQC | pH_5.0 | isotropic | pH_5.0_conditions |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - data analysis
Omega Spectrometer Operating Software vBeta 6.0.3b2, GE - collection
ANALYSIS, Boucher and Stevens - chemical shift assignment
NMR spectrometers:
- GE OMEGA 600 MHz
- GE OMEGA 500 MHz
Related Database Links:
BMRB | 16158 |
PDB | |
GB | AAA79833 AAA80243 AAA81366 AAA88821 AAI14108 |
PIR | S66396 |
REF | NP_001288146 NP_391988 XP_003364388 XP_004387928 XP_004401881 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts