BMRB Entry 1991
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR1991
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Title: Determinants of Protein Hyperthermostability: Purification and Amino Acid Sequence of Rubredoxin from the Hyperthermophilic Archaebacterium Pyrococcus furiosus and Secondary Structure of the Zinc Adduct by NMR
Deposition date: 1995-07-31 Original release date: 1995-07-31
Authors: Blake, Paul; Park, Jae-Bum; Bryant, Frank; Aono, Shigetoshi; Magnuson, Jon; Eccleston, Eric; Howard, James; Summers, Michael; Adams, Michael
Citation: Blake, Paul; Park, Jae-Bum; Bryant, Frank; Aono, Shigetoshi; Magnuson, Jon; Eccleston, Eric; Howard, James; Summers, Michael; Adams, Michael. "Determinants of Protein Hyperthermostability: Purification and Amino Acid Sequence of Rubredoxin from the Hyperthermophilic Archaebacterium Pyrococcus furiosus and Secondary Structure of the Zinc Adduct by NMR" Biochemistry 30, 10885-10895 (1991).
Assembly members:
rubredoxin, polymer, 53 residues, Formula weight is not available
Natural source: Common Name: hyperthermophylic archaebacter Taxonomy ID: 2261 Superkingdom: Archaea Kingdom: not available Genus/species: Pyrococcus furiosus
Experimental source: Production method: not available
Entity Sequences (FASTA):
rubredoxin: AKWVCKICGYIYDEDAGDPD
NGISPGTKFEELPDDWVCPI
CGAPKSEFEKLED
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 286 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | rubredoxin | 1 |
Entities:
Entity 1, rubredoxin 53 residues - Formula weight is not available
| 1 | ALA | LYS | TRP | VAL | CYS | LYS | ILE | CYS | GLY | TYR | ||||
| 2 | ILE | TYR | ASP | GLU | ASP | ALA | GLY | ASP | PRO | ASP | ||||
| 3 | ASN | GLY | ILE | SER | PRO | GLY | THR | LYS | PHE | GLU | ||||
| 4 | GLU | LEU | PRO | ASP | ASP | TRP | VAL | CYS | PRO | ILE | ||||
| 5 | CYS | GLY | ALA | PRO | LYS | SER | GLU | PHE | GLU | LYS | ||||
| 6 | LEU | GLU | ASP |
Samples:
sample_one:
sample_condition_set_one: pH: 6.3 na; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_condition_set_one |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz