BMRB Entry 4439
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4439
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Title: Hyperfine Cys Proton Signals from Oxidized Human Ferredoxin
Deposition date: 1999-10-15 Original release date: 2000-01-12
Authors: Xia, Bin; Jenk, Daniel; LeMaster, David; Westler, William; Markley, John
Citation: Xia, Bin; Jenk, Daniel; LeMaster, David; Westler, William; Markley, John. "Electron-Nuclear Interactions in two Prototypical [2Fe-2S] Proteins: Selective (Chiral) Deuteration and Analysis of 1H and 2H NMR Signals from the Alpha and Beta Hydrogens of Cysteinyl Residues that Ligate the Iron in the Active Sites of Human Ferredoxin and Anabaena 7120 Vegetative Ferredoxin" Arch. Biochem. Biophys. ., .-..
Assembly members:
Human Ferredoxin, polymer, 124 residues, Formula weight is not available
FE2/S2 (INORGANIC) CLUSTER, non-polymer, 175.820 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Human Ferredoxin: SSSEDKITVHFINRDGETLT
TKGKVGDSLLDVVVENNLDI
DGFGACEGTLACSTCHLIFE
DHIYEKLDAITDEENDMLDL
AYGLTDRSRLGCQICLTKSM
DNMTVRVPETVADARQSIDV
GKTS
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 12 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Human Ferredoxin | 1 |
2 | FES | 2 |
Entities:
Entity 1, Human Ferredoxin 124 residues - Formula weight is not available
1 | SER | SER | SER | GLU | ASP | LYS | ILE | THR | VAL | HIS | ||||
2 | PHE | ILE | ASN | ARG | ASP | GLY | GLU | THR | LEU | THR | ||||
3 | THR | LYS | GLY | LYS | VAL | GLY | ASP | SER | LEU | LEU | ||||
4 | ASP | VAL | VAL | VAL | GLU | ASN | ASN | LEU | ASP | ILE | ||||
5 | ASP | GLY | PHE | GLY | ALA | CYS | GLU | GLY | THR | LEU | ||||
6 | ALA | CYS | SER | THR | CYS | HIS | LEU | ILE | PHE | GLU | ||||
7 | ASP | HIS | ILE | TYR | GLU | LYS | LEU | ASP | ALA | ILE | ||||
8 | THR | ASP | GLU | GLU | ASN | ASP | MET | LEU | ASP | LEU | ||||
9 | ALA | TYR | GLY | LEU | THR | ASP | ARG | SER | ARG | LEU | ||||
10 | GLY | CYS | GLN | ILE | CYS | LEU | THR | LYS | SER | MET | ||||
11 | ASP | ASN | MET | THR | VAL | ARG | VAL | PRO | GLU | THR | ||||
12 | VAL | ALA | ASP | ALA | ARG | GLN | SER | ILE | ASP | VAL | ||||
13 | GLY | LYS | THR | SER |
Entity 2, FES - Fe2 S2 - 175.820 Da.
1 | FES |
Samples:
HuFd_oxidized: Human Ferredoxin2 3 mM
sample_conditions: pH*: 7.2 na; temperature: 293 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
not available | HuFd_oxidized | not available | sample_conditions |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 400 MHz