BMRB Entry 5782
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5782
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Title: 1H chemical shift assignment for the truncated form of the human beta2-microglobulin lacking of the first 3 residues PubMed: 14660575
Deposition date: 2003-04-24 Original release date: 2004-02-12
Authors: Viglino, Paolo; Corazza, Alessandra; Pettirossi, Fabio; Verdone, Giuliana; Esposito, Gennaro
Citation: Corazza, Alessandra; Pettirossi, Fabio; Viglino, Paolo; Verdone, Giuliana; Garcia, Julian; Dumy, Pascal; Giorgetti, Sofia; Mangione, Palma; Andreola, Alessia; Stoppini, Monica; Bellotti, Vittorio; Esposito, Gennaro. "Properties of some variants of human beta2-microglobulin and amyloidogenesis" J. Biol. Chem. ., .-..
Assembly members:
beta2-microglobulin, polymer, 97 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
beta2-microglobulin: MTPKIQVYSRHPAENGKSNF
LNCYVSGFHPSDIEVDLLKN
GERIEKVEHSDLSFSKDWSF
YLLYYTEFTPTEKDEYACRV
NHVTLSQPKIVKWDRDM
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 716 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | DN3beta2-microglobulin | 1 |
Entities:
Entity 1, DN3beta2-microglobulin 97 residues - Formula weight is not available
1 | MET | THR | PRO | LYS | ILE | GLN | VAL | TYR | SER | ARG | ||||
2 | HIS | PRO | ALA | GLU | ASN | GLY | LYS | SER | ASN | PHE | ||||
3 | LEU | ASN | CYS | TYR | VAL | SER | GLY | PHE | HIS | PRO | ||||
4 | SER | ASP | ILE | GLU | VAL | ASP | LEU | LEU | LYS | ASN | ||||
5 | GLY | GLU | ARG | ILE | GLU | LYS | VAL | GLU | HIS | SER | ||||
6 | ASP | LEU | SER | PHE | SER | LYS | ASP | TRP | SER | PHE | ||||
7 | TYR | LEU | LEU | TYR | TYR | THR | GLU | PHE | THR | PRO | ||||
8 | THR | GLU | LYS | ASP | GLU | TYR | ALA | CYS | ARG | VAL | ||||
9 | ASN | HIS | VAL | THR | LEU | SER | GLN | PRO | LYS | ILE | ||||
10 | VAL | LYS | TRP | ASP | ARG | ASP | MET |
Samples:
sample_1: beta2-microglobulin 0.86 mM; sodium phosphate 70 mM; sodium chloride 100 mM; H2O 100%
sample_2: beta2-microglobulin 0.7 mM; sodium phosphate 70 mM; sodium chloride 100 mM; D2O 95%; H2O 5%
Ex-cond_1: pH: 6.5 na; temperature: 310 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H-1H TOCSY | not available | not available | not available |
1H-1H NOESY | not available | not available | not available |
1H-1H DQF-COSY | not available | not available | not available |
Software:
FELIX v2000 -
NMR spectrometers:
- Bruker Avance 500 MHz