BMRB Entry 11010
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PDB ID: 2rmk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR11010
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Modha, Rakhee; Campbell, Louise; Nietlispach, Daniel; Buhecha, Heeran; Owen, Darerca; Mott, Helen. "The Rac1 polybasic region is required for interaction with its effector PRK1" J. Biol. Chem. 283, 1492-1500 (2008).
PubMed: 18006505
Assembly members:
Rac1, polymer, 192 residues, 21477.398 Da.
PRK1_HR1b, polymer, 81 residues, 9019.512 Da.
GCP, non-polymer, 521.208 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T
Entity Sequences (FASTA):
Rac1: MQAIKCVVVGDGAVGKTCLL
ISYTTNAFPGEYIPTVFDNY
SANVMVDGKPVNLGLWDTAG
LEDYDRLRPLSYPQTDVFLI
CFSLVSPASFENVRAKWYPE
VRHHCPNTPIILVGTKLDLR
DDKDTIEKLKEKKLTPITYP
QGLAMAKEIGAVKYLECSAL
TQRGLKTVFDEAIRAVLCPP
PVKKRKRKCLLL
PRK1_HR1b: GIPATNLSRVAGLEKQLAIE
LKVKQGAENMIQTYSNGSTK
DRKLLLTAQQMLQDSKTKID
IIRMQLRRALQADQLENQAA
P
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 970 |
| 15N chemical shifts | 248 |
| 1H chemical shifts | 1666 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rac1 | 1 |
| 2 | PRK1 HR1b | 2 |
| 3 | PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER | 3 |
Entities:
Entity 1, Rac1 192 residues - 21477.398 Da.
| 1 | MET | GLN | ALA | ILE | LYS | CYS | VAL | VAL | VAL | GLY | ||||
| 2 | ASP | GLY | ALA | VAL | GLY | LYS | THR | CYS | LEU | LEU | ||||
| 3 | ILE | SER | TYR | THR | THR | ASN | ALA | PHE | PRO | GLY | ||||
| 4 | GLU | TYR | ILE | PRO | THR | VAL | PHE | ASP | ASN | TYR | ||||
| 5 | SER | ALA | ASN | VAL | MET | VAL | ASP | GLY | LYS | PRO | ||||
| 6 | VAL | ASN | LEU | GLY | LEU | TRP | ASP | THR | ALA | GLY | ||||
| 7 | LEU | GLU | ASP | TYR | ASP | ARG | LEU | ARG | PRO | LEU | ||||
| 8 | SER | TYR | PRO | GLN | THR | ASP | VAL | PHE | LEU | ILE | ||||
| 9 | CYS | PHE | SER | LEU | VAL | SER | PRO | ALA | SER | PHE | ||||
| 10 | GLU | ASN | VAL | ARG | ALA | LYS | TRP | TYR | PRO | GLU | ||||
| 11 | VAL | ARG | HIS | HIS | CYS | PRO | ASN | THR | PRO | ILE | ||||
| 12 | ILE | LEU | VAL | GLY | THR | LYS | LEU | ASP | LEU | ARG | ||||
| 13 | ASP | ASP | LYS | ASP | THR | ILE | GLU | LYS | LEU | LYS | ||||
| 14 | GLU | LYS | LYS | LEU | THR | PRO | ILE | THR | TYR | PRO | ||||
| 15 | GLN | GLY | LEU | ALA | MET | ALA | LYS | GLU | ILE | GLY | ||||
| 16 | ALA | VAL | LYS | TYR | LEU | GLU | CYS | SER | ALA | LEU | ||||
| 17 | THR | GLN | ARG | GLY | LEU | LYS | THR | VAL | PHE | ASP | ||||
| 18 | GLU | ALA | ILE | ARG | ALA | VAL | LEU | CYS | PRO | PRO | ||||
| 19 | PRO | VAL | LYS | LYS | ARG | LYS | ARG | LYS | CYS | LEU | ||||
| 20 | LEU | LEU |
Entity 2, PRK1 HR1b 81 residues - 9019.512 Da.
Residues 119-121 represent a non-native affinity tag
| 1 | GLY | ILE | PRO | ALA | THR | ASN | LEU | SER | ARG | VAL | ||||
| 2 | ALA | GLY | LEU | GLU | LYS | GLN | LEU | ALA | ILE | GLU | ||||
| 3 | LEU | LYS | VAL | LYS | GLN | GLY | ALA | GLU | ASN | MET | ||||
| 4 | ILE | GLN | THR | TYR | SER | ASN | GLY | SER | THR | LYS | ||||
| 5 | ASP | ARG | LYS | LEU | LEU | LEU | THR | ALA | GLN | GLN | ||||
| 6 | MET | LEU | GLN | ASP | SER | LYS | THR | LYS | ILE | ASP | ||||
| 7 | ILE | ILE | ARG | MET | GLN | LEU | ARG | ARG | ALA | LEU | ||||
| 8 | GLN | ALA | ASP | GLN | LEU | GLU | ASN | GLN | ALA | ALA | ||||
| 9 | PRO |
Entity 3, PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER - C11 H18 N5 O13 P3 - 521.208 Da.
| 1 | GCP |
Related Database Links:
| SP | P63000 P62998 P62999 P63000 P63001 Q6RUV5 |
| PDB | 1E96 1FOE 1G4U 1HE1 1HH4 1I4D 1I4L 1I4T 1MH1 2FJU 2H7V 2NZ8 2P2L 2RMK 2VRW 2WKP 2WKQ 2WKR 2YIN 3B13 3BJI 3RYT 3SBD 3SBE 3SU8 3SUA 3TH5 4GZL 4GZM 4YON |
| DBJ | BAB25667 BAB26027 BAB69451 BAC16311 BAC28767 |
| EMBL | CAA39801 CAA40545 CAB53579 CAJ83626 CDQ72552 |
| GB | AAA36537 AAA36544 AAB22206 AAC18960 AAD50299 |
| REF | NP_001003274 NP_001004840 NP_001034907 NP_001084224 NP_001089332 |
| TPG | DAA15019 |
| SWS | Q16512 |
| AlphaFold | Q9BTB4 P62998 P62999 P63000 P63001 Q6RUV5 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks