BMRB Entry 11060

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PDB ID: 2rpb
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR11060
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Unusual Thermal Disassembly of the SPFH Domain Oligomer from Pyrococcus horikoshii

Deposition date:

2008-11-26

Original release date:

2010-01-11

Authors:

Kuwahara, Yohta; Unzai, Satoru; Nagata, Takashi; Hiroaki, Yoko; Yokoyama, Hideshi; Matsui, Ikuo; Ikegami, Takahisa; Fujiyoshi, Yoshinori; Hiroaki, Hidekazu

Citation:

Citation: Kuwahara, Yohta; Unzai, Satoru; Nagata, Takashi; Hiroaki, Yoko; Yokoyama, Hideshi; Matsui, Ikuo; Ikegami, Takahisa; Fujiyoshi, Yoshinori; Hiroaki, Hidekazu. "Unusual Thermal Disassembly of the SPFH Domain Oligomer from Pyrococcus horikoshii" Biophysical J. 97, 2034-2043 (2009).
PubMed: 19804735

Assembly members:

Assembly members:
SPFH domain, polymer, 113 residues, 12868.851 Da.

Natural source:

Natural source: Common Name: Pyrococcus horikoshii Taxonomy ID: 53953 Superkingdom: Archaea Kingdom: not available Genus/species: Pyrococcus horikoshii

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SPFH domain: GSDHVDLREHVIDVPPQEVI CKDNVVVTVDAVVYYQVIDP VKAVYNVSDFLMAIVKLAQT NLRAIIGEMELDETLSGRDI INARLREELDKITDRWGVKI TRVEIQRIDPPKD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	395
15N chemical shifts	115
1H chemical shifts	807

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SPFH domain	1

Entities:

Entity 1, SPFH domain 113 residues - 12868.851 Da.

Residues 62-65 represent cloning artifacts

1

GLY

SER

ASP

HIS

VAL

ASP

LEU

ARG

GLU

HIS

2

VAL

ILE

ASP

VAL

PRO

GLN

GLU

VAL

ILE

3

CYS

LYS

ASP

ASN

VAL

THR

VAL

ASP

4

ALA

VAL

TYR

GLN

VAL

ILE

ASP

PRO

5

VAL

LYS

ALA

VAL

TYR

ASN

VAL

SER

ASP

PHE

6

LEU

MET

ALA

ILE

VAL

LYS

LEU

ALA

GLN

THR

7

ASN

LEU

ARG

ALA

ILE

GLY

GLU

MET

GLU

8

LEU

ASP

GLU

THR

LEU

SER

GLY

ARG

ASP

ILE

9

ILE

ASN

ALA

ARG

LEU

ARG

GLU

LEU

ASP

10

LYS

ILE

THR

ASP

ARG

TRP

GLY

VAL

LYS

ILE

11

THR

ARG

VAL

GLU

ILE

GLN

ARG

ILE

ASP

PRO

12

PRO

LYS

ASP

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CC(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCC(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 13C-editted NOESY	sample_2	isotropic	sample_conditions_1
3D 15N-editted NOESY	sample_1	isotropic	sample_conditions_1

PDB	2RPB
DBJ	BAA29558
EMBL	CAB50468
GB	AEC52040
REF	WP_013748594 WP_048053138 WP_048147080
TPE	CCE71018

BMRB Entry 11060

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links: