BMRB Entry 11115
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PDB ID: 2rr4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR11115
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: He, Fahu; Umehara, Takashi; Saito, Kohei; Harada, Takushi; Watanabe, Satoru; Yabuki, Takashi; Kigawa, Takanori; Takahashi, Mari; Kuwasako, Kanako; Tsuda, Kengo; Matsuda, Takayoshi; Aoki, Masaaki; Seki, Eiko; Kobayashi, Naohiro; Guntert, Peter; Yokoyama, Shigeyuki; Muto, Yutaka. "Structural insight into the zinc finger CW domain as a histone modification reader" Structure 18, 1127-1139 (2010).
PubMed: 20826339
Assembly members:
zf-CW domain, polymer, 69 residues, 7648.395 Da.
H3K4me3 peptide, polymer, 10 residues, 1192.423 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: E. coli - cell free Vector: P060116-12
Entity Sequences (FASTA):
zf-CW domain: GSSGSSGEISGFGQCLVWVQ
CSFPNCGKWRRLCGNIDPSV
LPDNWSCDQNTDVQYNRCDI
PEETWTGLE
H3K4me3 peptide: ARTXQTARKS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 272 |
| 15N chemical shifts | 75 |
| 1H chemical shifts | 496 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | zf-CW domain | 1 |
| 2 | H3K4me3 peptide | 2 |
| 3 | ZINC ION | 3 |
Entities:
Entity 1, zf-CW domain 69 residues - 7648.395 Da.
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLU | ILE | SER | ||||
| 2 | GLY | PHE | GLY | GLN | CYS | LEU | VAL | TRP | VAL | GLN | ||||
| 3 | CYS | SER | PHE | PRO | ASN | CYS | GLY | LYS | TRP | ARG | ||||
| 4 | ARG | LEU | CYS | GLY | ASN | ILE | ASP | PRO | SER | VAL | ||||
| 5 | LEU | PRO | ASP | ASN | TRP | SER | CYS | ASP | GLN | ASN | ||||
| 6 | THR | ASP | VAL | GLN | TYR | ASN | ARG | CYS | ASP | ILE | ||||
| 7 | PRO | GLU | GLU | THR | TRP | THR | GLY | LEU | GLU |
Entity 2, H3K4me3 peptide 10 residues - 1192.423 Da.
contains N-TRIMETHYLLYSINE, instead of 4K
| 1 | ALA | ARG | THR | M3L | GLN | THR | ALA | ARG | LYS | SER |
Entity 3, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Related Database Links:
| BMRB | 11358 11359 11360 11361 11362 |
| PDB | 2E61 2RR4 |
| DBJ | BAA91424 BAC04028 BAG62414 BAG63489 |
| EMBL | CAB66669 |
| GB | AAH02725 AIC60275 EAW76538 EAW76539 EAW76540 |
| REF | NP_001244937 NP_060454 XP_002803268 XP_003318693 XP_003318694 |
| SP | Q9H0M4 |
| AlphaFold | Q9H0M4 Q93081 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated peaks