BMRB Entry 11508
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PDB ID: 2rsy
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11508
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Tanaka, Hiroaki; Akagi, Ken-ichi; Oneyama, Chitose; Tanaka, Masakazu; Sasaki, Yuichi; Kanou, Takashi; Lee, Young-Ho; Yokogawa, Daisuke; Debenecker, Marc-Werner; Nakagawa, Atsushi; Okada, Masato; Ikegami, Takahisa. "A new SH2 domain binding mode of C-terminal Src kianse to Cbp/PAG"" .
Assembly members:
Csk_SH2, polymer, 99 residues, 11473.218 Da.
Cbp_phosphopeptide, polymer, 38 residues, 4263.602 Da.
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukayota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6p-1
Entity Sequences (FASTA):
Csk_SH2: GPLGSMPWFHGKITREQAER
LLYPPETGLFLVRESTNYPG
DYTLCVSCEGKVEHYRIMYH
ASKLSIDEEVYFENLMQLVE
HYTTDADGLCTRLIKPKVM
Cbp_phosphopeptide: GPLGSKRFSSLSYKSREEDP
TLTEEEISAMXSSVNKPG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 581 |
| 15N chemical shifts | 137 |
| 1H chemical shifts | 887 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 2 |
Entities:
Entity 1, entity_1 99 residues - 11473.218 Da.
Residues 1-5 represent a non native affinity tag
| 1 | GLY | PRO | LEU | GLY | SER | MET | PRO | TRP | PHE | HIS | ||||
| 2 | GLY | LYS | ILE | THR | ARG | GLU | GLN | ALA | GLU | ARG | ||||
| 3 | LEU | LEU | TYR | PRO | PRO | GLU | THR | GLY | LEU | PHE | ||||
| 4 | LEU | VAL | ARG | GLU | SER | THR | ASN | TYR | PRO | GLY | ||||
| 5 | ASP | TYR | THR | LEU | CYS | VAL | SER | CYS | GLU | GLY | ||||
| 6 | LYS | VAL | GLU | HIS | TYR | ARG | ILE | MET | TYR | HIS | ||||
| 7 | ALA | SER | LYS | LEU | SER | ILE | ASP | GLU | GLU | VAL | ||||
| 8 | TYR | PHE | GLU | ASN | LEU | MET | GLN | LEU | VAL | GLU | ||||
| 9 | HIS | TYR | THR | THR | ASP | ALA | ASP | GLY | LEU | CYS | ||||
| 10 | THR | ARG | LEU | ILE | LYS | PRO | LYS | VAL | MET |
Entity 2, entity_2 38 residues - 4263.602 Da.
Residues 1-5 represent a non-native affinity tag
| 1 | GLY | PRO | LEU | GLY | SER | LYS | ARG | PHE | SER | SER | ||||
| 2 | LEU | SER | TYR | LYS | SER | ARG | GLU | GLU | ASP | PRO | ||||
| 3 | THR | LEU | THR | GLU | GLU | GLU | ILE | SER | ALA | MET | ||||
| 4 | PTR | SER | SER | VAL | ASN | LYS | PRO | GLY |
Related Database Links:
| UNP | P32577 Q9JM80 |
| PDB | 1K9A 2RSY |
| DBJ | BAE23297 BAE33565 BAE42047 BAG60052 BAA95413 BAC30545 BAC40842 BAE33539 BAE42829 |
| EMBL | CAA41484 |
| GB | AAA18766 AAH18394 AAH52006 AAH98863 AAX90624 AAG44565 AAI45329 AAI45762 AAI45764 EDL05179 |
| PRF | 1709363A |
| REF | NP_001025210 NP_001291690 NP_031809 XP_003463668 XP_004628790 NP_001181960 NP_071589 NP_444412 XP_005066844 XP_006530170 |
| SP | P32577 P41241 Q3U1F9 Q9JM80 |
| AlphaFold | Q4G003 P32577 P41241 Q9JM80 Q3U1F9 Q9JM80 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks