BMRB Entry 11523

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PDB ID: 2rt3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11523
MolProbity Validation Chart

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Title:
Solution structure of the second RRM domain of Nrd1
Deposition date:
2013-04-16
Original release date:
2014-04-14
Authors:
Kobayashi, Ayaho; Kanaba, Teppei; Mishima, Masaki
Citation:

Citation: Kobayashi, Ayaho; Kanaba, Teppei; Satoh, Ryosuke; Fujiwara, Toshinobu; Ito, Yutaka; Sugiura, Reiko; Mishima, Masaki. "Structure of the second RRM domain of Nrd1, a fission yeast MAPK target RNA binding protein, and implication for its RNA recognition and regulation"  Biochem. Biophys. Res. Commun. 437, 12-17 (2013).
PubMed: 23770370

Assembly members:

Assembly members:
Nrd1_RRM2, polymer, 97 residues, 10793.440 Da.

Natural source:

Natural source:   Common Name: fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-49b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nrd1_RRM2: NSASNSSVLLAVQQSGACRN VFLGNLPNGITEDEIREDLE PFGPIDQIKIVTERNIAFVH FLNIAAAIKAVQELPLNPKW SKRRIYYGRDRCAVGLK

Data sets:
Data typeCount
13C chemical shifts410
15N chemical shifts93
1H chemical shifts652

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Nrd1_RRM21

Entities:

Entity 1, Nrd1_RRM2 97 residues - 10793.440 Da.

1   ASNSERALASERASNSERSERVALLEULEU
2   ALAVALGLNGLNSERGLYALACYSARGASN
3   VALPHELEUGLYASNLEUPROASNGLYILE
4   THRGLUASPGLUILEARGGLUASPLEUGLU
5   PROPHEGLYPROILEASPGLNILELYSILE
6   VALTHRGLUARGASNILEALAPHEVALHIS
7   PHELEUASNILEALAALAALAILELYSALA
8   VALGLNGLULEUPROLEUASNPROLYSTRP
9   SERLYSARGARGILETYRTYRGLYARGASP
10   ARGCYSALAVALGLYLEULYS

Related Database Links:

PDB 2RT3

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