BMRB Entry 11569
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PDB ID: 2ruh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11569
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Nagata, Takashi; Shirakawa, Kie; Kobayashi, Naohiro; Shiheido, Hidekazu; Horisawa, Kenichi; Katahira, Masato; Doi, Nobuhide; Yanagawa, Hiroshi. "Structural Basis for Inhibition of the MDM2:p53 Interaction by an Optimized MDM2-Binding Peptide Selected with mRNA Display" Plos One 9, e109163-e109163 (2014).
PubMed: 25275651
Assembly members:
MIP-MDM2, polymer, 131 residues, 15031.517 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b
Entity Sequences (FASTA):
MIP-MDM2: MPRFWEYWLRLMEGGGENLY
FQGMSVPTDGAVTTSQIPAS
EQETLVRPKPLLLKLLKSVG
AQKDTYTMKEVLFYLGQYIM
TKRLYDEKQQHIVYCSNDLL
GDLFGVPSFSVKEHRKIYTM
MASMTGGQQMG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 575 |
| 15N chemical shifts | 115 |
| 1H chemical shifts | 863 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 131 residues - 15031.517 Da.
Residues -11Met to 1Glu corresponds to 0Met to 12Glu of MIP peptide, residues 2Gly to 10Gln represents TEV cleavage sequence, residues 11Gly to 108Met corresponds to MDM2, and residues M109 to 119Gly represents T7tag."
| 1 | MET | PRO | ARG | PHE | TRP | GLU | TYR | TRP | LEU | ARG | ||||
| 2 | LEU | MET | GLU | GLY | GLY | GLY | GLU | ASN | LEU | TYR | ||||
| 3 | PHE | GLN | GLY | MET | SER | VAL | PRO | THR | ASP | GLY | ||||
| 4 | ALA | VAL | THR | THR | SER | GLN | ILE | PRO | ALA | SER | ||||
| 5 | GLU | GLN | GLU | THR | LEU | VAL | ARG | PRO | LYS | PRO | ||||
| 6 | LEU | LEU | LEU | LYS | LEU | LEU | LYS | SER | VAL | GLY | ||||
| 7 | ALA | GLN | LYS | ASP | THR | TYR | THR | MET | LYS | GLU | ||||
| 8 | VAL | LEU | PHE | TYR | LEU | GLY | GLN | TYR | ILE | MET | ||||
| 9 | THR | LYS | ARG | LEU | TYR | ASP | GLU | LYS | GLN | GLN | ||||
| 10 | HIS | ILE | VAL | TYR | CYS | SER | ASN | ASP | LEU | LEU | ||||
| 11 | GLY | ASP | LEU | PHE | GLY | VAL | PRO | SER | PHE | SER | ||||
| 12 | VAL | LYS | GLU | HIS | ARG | LYS | ILE | TYR | THR | MET | ||||
| 13 | MET | ALA | SER | MET | THR | GLY | GLY | GLN | GLN | MET | ||||
| 14 | GLY |
Related Database Links:
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