BMRB Entry 15170

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15170

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Title:
Backbone assignments of DNA methyltransferase M.HhaI
Deposition date:
2007-03-09
Original release date:
2007-06-06
Authors:
Zhou, Hongjun; Shatz, Whitney; Purdy, Matthew; Fera, Nick; Dahlquist, Frederick; Reich, Norbert
Citation:

Citation: Zhou, Hongjun; Shatz, Whitney; Purdy, Matthew; Fera, Nick; Dahlquist, Frederick; Reich, Norbert. "Long-range structural and dynamical changes induced by cofactor binding in DNA methyltransferase M.HhaI."  Biochemistry 46, 7261-7268 (2007).
PubMed: 17523600

Assembly members:

Assembly members:
M.HhaI, polymer, 331 residues, Formula weight is not available
SAH, non-polymer, 384.411 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
M.HhaI: MIEIKDKQLTGLRFIDLFAG LGGFRLALESCGAECVYSNE WDKYAQEVYEMNFGEKPEGD ITQVNEKTIPDHDILCAGFP CQAFSISGKQKGFEDSRGTL FFDIARIVREKKPKVVFMEN VKNFASHDNGNTLEVVKNTM NELDYSFHAKVLNALDYGIP QKRERIYMICFRNDLNIQNF QFPKPFELNTFVKDLLLPDS EVEHLVIDRKDLVMTNQEIE QTTPKTVRLGIVGKGGQGER IYSTRGIAITLSAYGGGIFA KTGGYLVNGKTRKLHPRECA RVMGYPDSYKVHPSTSQAYK QFGNSVVINVLQYIAYNIGS SLNLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts483
15N chemical shifts242
1H chemical shifts242

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer1
2SAH2

Entities:

Entity 1, monomer 331 residues - Formula weight is not available

1   METILEGLUILELYSASPLYSGLNLEUTHR
2   GLYLEUARGPHEILEASPLEUPHEALAGLY
3   LEUGLYGLYPHEARGLEUALALEUGLUSER
4   CYSGLYALAGLUCYSVALTYRSERASNGLU
5   TRPASPLYSTYRALAGLNGLUVALTYRGLU
6   METASNPHEGLYGLULYSPROGLUGLYASP
7   ILETHRGLNVALASNGLULYSTHRILEPRO
8   ASPHISASPILELEUCYSALAGLYPHEPRO
9   CYSGLNALAPHESERILESERGLYLYSGLN
10   LYSGLYPHEGLUASPSERARGGLYTHRLEU
11   PHEPHEASPILEALAARGILEVALARGGLU
12   LYSLYSPROLYSVALVALPHEMETGLUASN
13   VALLYSASNPHEALASERHISASPASNGLY
14   ASNTHRLEUGLUVALVALLYSASNTHRMET
15   ASNGLULEUASPTYRSERPHEHISALALYS
16   VALLEUASNALALEUASPTYRGLYILEPRO
17   GLNLYSARGGLUARGILETYRMETILECYS
18   PHEARGASNASPLEUASNILEGLNASNPHE
19   GLNPHEPROLYSPROPHEGLULEUASNTHR
20   PHEVALLYSASPLEULEULEUPROASPSER
21   GLUVALGLUHISLEUVALILEASPARGLYS
22   ASPLEUVALMETTHRASNGLNGLUILEGLU
23   GLNTHRTHRPROLYSTHRVALARGLEUGLY
24   ILEVALGLYLYSGLYGLYGLNGLYGLUARG
25   ILETYRSERTHRARGGLYILEALAILETHR
26   LEUSERALATYRGLYGLYGLYILEPHEALA
27   LYSTHRGLYGLYTYRLEUVALASNGLYLYS
28   THRARGLYSLEUHISPROARGGLUCYSALA
29   ARGVALMETGLYTYRPROASPSERTYRLYS
30   VALHISPROSERTHRSERGLNALATYRLYS
31   GLNPHEGLYASNSERVALVALILEASNVAL
32   LEUGLNTYRILEALATYRASNILEGLYSER
33   SERLEUASNLEUGLUHISHISHISHISHIS
34   HIS

Entity 2, SAH - C14 H20 N6 O5 S - 384.411 Da.

1   SAH

Related Database Links:

BMRB 16395
PDB 10MH 1FJX 1HMY 1M0E 1MHT 1SKM 1SVU 2C7O 2C7P 2C7Q 2C7R 2HMY 2HR1 2I9K 2UYC 2UYH 2UZ4 2Z6A 2Z6Q 2Z6U 2ZCJ 3EEO 3MHT 4MHT 5MHT 6MHT 7MHT 8MHT 9MHT
GB AAA24989 EIJ69210
REF WP_005706946
SP P05102
AlphaFold P05102

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks