BMRB Entry 15378

Title:
NMR structure of the S. aureus VraR DNA binding domain
Deposition date:
2007-07-13
Original release date:
2008-03-13
Authors:
Donaldson, Logan
Citation:

Citation: Donaldson, Logan. "The NMR Structure of the Staphylococcus aureus Response Regulator VraR DNA Binding Domain Reveals a Dynamic Relationship between It and Its Associated Receiver Domain"  Biochemistry 47, 3379-3388 (2008).
PubMed: 18293926

Assembly members:

Assembly members:
VraR, polymer, 93 residues, 10625.2 Da.

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
13C chemical shifts239
15N chemical shifts55
1H chemical shifts366

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1VraR1

Entities:

Entity 1, VraR 93 residues - 10625.2 Da.

The first 20 residues are a His6 affinity tag and thrombin protease recognition site derived from pET15b (Novagen). The next 6 residues are derived from the native protein but not included in the structure determination

1   GLYSERSERHISHISHISHISHISHISSER
2   SERGLYLEUVALPROARGGLYSERHISMET
3   LYSLYSARGALAGLULEUGLULEUTYRGLU
4   METLEUTHRGLUARGGLUMETGLUILELEU
5   LEULEUILEALALYSGLYTYRSERASNGLN
6   GLUILEALASERALASERHISILETHRILE
7   LYSTHRVALLYSTHRHISVALSERASNILE
8   LEUSERLYSLEUGLUVALGLNASPARGTHR
9   GLNALAVALILETYRALAPHEGLNHISASN
10   LEUILEGLN

Related Database Links:

GB 14247656 47208328 EUS58931
BMRB 11024
PDB 2RNJ

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks