BMRB Entry 15525
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15525
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Citation: Fenwick, R Bryn; Prasannan, Sunil; Campbell, Louise; Evetts, Katrina; Nietlispach, Daniel; Owen, Darerca; Mott, Helen. "Resonance assignments for the RLIP76 Ral binding domain in its free form and in complex with the small G protein RalB" Biomol. NMR Assignments 2, 191-194 (2008).
PubMed: 19636902
Assembly members:
RLIP76, polymer, 56 residues, Formula weight is not available
RalB, polymer, 188 residues, Formula weight is not available
GNP, non-polymer, 522.196 Da.
MG, non-polymer, 24.305 Da.
HOH, non-polymer, 18.015 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 1007 |
| 15N chemical shifts | 250 |
| 1H chemical shifts | 1707 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RLIP76 | 1 |
| 2 | RalB | 2 |
| 3 | GMPPNP | 3 |
| 4 | Magnesium ion | 4 |
| 5 | BOUND WATER, 1 | 5 |
| 6 | BOUND WATER, 2 | 5 |
Entities:
Entity 1, RLIP76 56 residues - Formula weight is not available
The first two residues are a tag remnant. The protein starts at E393 and ends at A446
| 1 | GLY | SER | GLU | THR | GLN | ALA | GLY | ILE | LYS | GLU | ||||
| 2 | GLU | ILE | ARG | ARG | GLN | GLU | PHE | LEU | LEU | ASN | ||||
| 3 | SER | LEU | HIS | ARG | ASP | LEU | GLN | GLY | GLY | ILE | ||||
| 4 | LYS | ASP | LEU | SER | LYS | GLU | GLU | ARG | LEU | TRP | ||||
| 5 | GLU | VAL | GLN | ARG | ILE | LEU | THR | ALA | LEU | LYS | ||||
| 6 | ARG | LYS | LEU | ARG | GLU | ALA |
Entity 2, RalB 188 residues - Formula weight is not available
The first three residues are a tag remnant. The protein starts at M1 and ends at K185
| 1 | GLY | SER | HIS | MET | ALA | ALA | ASN | LYS | SER | LYS | ||||
| 2 | GLY | GLN | SER | SER | LEU | ALA | LEU | HIS | LYS | VAL | ||||
| 3 | ILE | MET | VAL | GLY | SER | GLY | GLY | VAL | GLY | LYS | ||||
| 4 | SER | ALA | LEU | THR | LEU | GLN | PHE | MET | TYR | ASP | ||||
| 5 | GLU | PHE | VAL | GLU | ASP | TYR | GLU | PRO | THR | LYS | ||||
| 6 | ALA | ASP | SER | TYR | ARG | LYS | LYS | VAL | VAL | LEU | ||||
| 7 | ASP | GLY | GLU | GLU | VAL | GLN | ILE | ASP | ILE | LEU | ||||
| 8 | ASP | THR | ALA | GLY | LEU | GLU | ASP | TYR | ALA | ALA | ||||
| 9 | ILE | ARG | ASP | ASN | TYR | PHE | ARG | SER | GLY | GLU | ||||
| 10 | GLY | PHE | LEU | LEU | VAL | PHE | SER | ILE | THR | GLU | ||||
| 11 | HIS | GLU | SER | PHE | THR | ALA | THR | ALA | GLU | PHE | ||||
| 12 | ARG | GLU | GLN | ILE | LEU | ARG | VAL | LYS | ALA | GLU | ||||
| 13 | GLU | ASP | LYS | ILE | PRO | LEU | LEU | VAL | VAL | GLY | ||||
| 14 | ASN | LYS | SER | ASP | LEU | GLU | GLU | ARG | ARG | GLN | ||||
| 15 | VAL | PRO | VAL | GLU | GLU | ALA | ARG | SER | LYS | ALA | ||||
| 16 | GLU | GLU | TRP | GLY | VAL | GLN | TYR | VAL | GLU | THR | ||||
| 17 | SER | ALA | LYS | THR | ARG | ALA | ASN | VAL | ASP | LYS | ||||
| 18 | VAL | PHE | PHE | ASP | LEU | MET | ARG | GLU | ILE | ARG | ||||
| 19 | THR | LYS | LYS | MET | SER | GLU | ASN | LYS |
Entity 3, GMPPNP - C10 H17 N6 O13 P3 - 522.196 Da.
| 1 | GNP |
Entity 4, Magnesium ion - Mg - 24.305 Da.
| 1 | MG |
Entity 5, BOUND WATER, 1 - H2 O - 18.015 Da.
| 1 | HOH |
Related Database Links:
| BMRB | 15524 15230 15803 |
| PDB | 2KWH 2KWI 2KE5 |
| GB | AAI45321 AAA60250 AAH18163 AAI46174 AAM12625 AAP35599 |
| REF | XP_004656898 NP_001091454 NP_001126995 NP_001244728 NP_002872 XP_001492315 |
| DBJ | BAE02438 BAG35360 BAG64302 BAJ20798 |
| EMBL | CAA33119 CAH93398 |
| SP | P11234 Q4R379 Q5R4B8 |
| TPG | DAA32595 |
| AlphaFold | P11234 Q4R379 Q5R4B8 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks