BMRB Entry 15685

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15685

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Title:
Backbone and side chain 1H, 13C, and 15N Chemical Shift Assignments for the complex of Epidermal Growth Factor-like 2 domain from Microneme Protein 6 (Toxoplasma gondii) with the Galectin-like domain of Microneme protein 1
Deposition date:
2008-03-17
Original release date:
2008-10-03
Authors:
Sawmynaden, Kovilen; Saouros, Savvas; Marchant, Jan; Simpson, Peter; Matthews, Stephen
Citation:

Citation: Sawmynaden, Kovilen; Saouros, Savvas; Marchant, Jan; Simpson, Peter; Matthews, Stephen. "Complete NMR assignments for the second EGF domain of MIC6 from Toxoplasma gondii and re-assignment in complex with the galectin-like domain of MIC1"  Biomol. NMR Assignments 2, 187-189 (2008).
PubMed: 19636901

Assembly members:

Assembly members:
Epidermal_Growth_Factor-like_domain_2, polymer, 61 residues, 6362.06 Da.
Microneme_Protein_6, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Toxoplasma gondii   Taxonomy ID: 5811   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Toxoplasma gondii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-32 Xa/LIC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Epidermal_Growth_Factor-like_domain_2: MANFVQLSETPAACSSNPCG PEAAGTCKETNSGYICRCNQ GYRISLDGTGNVTCIVRQES G
Microneme_Protein_6: MKTEIHGDSTKATLEEGQQL TLTFISTKLDVAVGSCHSLV ANFLDGFLKFQTGSNSAFDV VEVEEPAGPAVLTIGLGHKG RLAVVLDYTRLNAALGSAAY VVEDSGCSSSEEVSFQGVGS GATLVVTTLGESPTAVSA

Data sets:
Data typeCount
13C chemical shifts764
15N chemical shifts180
1H chemical shifts1169

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Epidermal Growth Factor-like domain 21
2Galectin-like domain (MIC1)2

Entities:

Entity 1, Epidermal Growth Factor-like domain 2 61 residues - 6362.06 Da.

1   METALAASNPHEVALGLNLEUSERGLUTHR
2   PROALAALACYSSERSERASNPROCYSGLY
3   PROGLUALAALAGLYTHRCYSLYSGLUTHR
4   ASNSERGLYTYRILECYSARGCYSASNGLN
5   GLYTYRARGILESERLEUASPGLYTHRGLY
6   ASNVALTHRCYSILEVALARGGLNGLUSER
7   GLY

Entity 2, Galectin-like domain (MIC1) 138 residues - Formula weight is not available

1   METLYSTHRGLUILEHISGLYASPSERTHR
2   LYSALATHRLEUGLUGLUGLYGLNGLNLEU
3   THRLEUTHRPHEILESERTHRLYSLEUASP
4   VALALAVALGLYSERCYSHISSERLEUVAL
5   ALAASNPHELEUASPGLYPHELEULYSPHE
6   GLNTHRGLYSERASNSERALAPHEASPVAL
7   VALGLUVALGLUGLUPROALAGLYPROALA
8   VALLEUTHRILEGLYLEUGLYHISLYSGLY
9   ARGLEUALAVALVALLEUASPTYRTHRARG
10   LEUASNALAALALEUGLYSERALAALATYR
11   VALVALGLUASPSERGLYCYSSERSERSER
12   GLUGLUVALSERPHEGLNGLYVALGLYSER
13   GLYALATHRLEUVALVALTHRTHRLEUGLY
14   GLUSERPROTHRALAVALSERALA

Related Database Links:

BMRB 15682 15692 6376
PDB 2K2S 2K2T 2BVB
EMBL CAA96466
GB EPR59679 EPT28103 ESS33997 KFG30856 KFG41657
REF XP_002368531
SP O00834
AlphaFold O00834

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks