BMRB Entry 15706
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15706
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NMR-STAR v3 text file.
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Citation: Chen, Yu-Jen; Wu, Kuen-Phon; Kim, Seho; Falzon, Liliana; Inouye, Masayori; Baum, Jean. "Backbone NMR assignments of DFP-inhibited mature subtilisin E" Biomol. NMR Assignments 2, 131-133 (2008).
PubMed: 19636887
Assembly members:
Subtilisin_E, polymer, 275 residues, Formula weight is not available
DFP, non-polymer, Formula weight is not available
CA, non-polymer, Formula weight is not available
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a
Entity Sequences (FASTA):
Subtilisin_E: AQSFPYGISQIKAPALHSQG
YTGSNVKVAVIDSGIDSSHP
DLNVRGGASFVPSETNPYQD
GSSHGTHVAGTIAALNNSIG
VLGVAPSASLYAVKVLDSTG
SGQYSWIINGIEWAISNNMD
VINMSLGGPTGSTALKTVVD
KAVSSGIVVAAAAGNEGSSG
STSTVGYPAKYPSTIAVGAV
NSSNQRASFSSAGSELDVMA
PGVSIQSTLPGGTYGAYNGT
SMATPHVAGAAALILSKHPT
WTNAQVRDRLESTATYLGNS
FYYGKGLINVQAAAQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 776 |
| 15N chemical shifts | 252 |
| 1H chemical shifts | 252 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Subtilisin E | 1 |
| 2 | DFP | 2 |
| 3 | Calcium | 3 |
Entities:
Entity 1, Subtilisin E 275 residues - Formula weight is not available
| 1 | ALA | GLN | SER | PHE | PRO | TYR | GLY | ILE | SER | GLN | ||||
| 2 | ILE | LYS | ALA | PRO | ALA | LEU | HIS | SER | GLN | GLY | ||||
| 3 | TYR | THR | GLY | SER | ASN | VAL | LYS | VAL | ALA | VAL | ||||
| 4 | ILE | ASP | SER | GLY | ILE | ASP | SER | SER | HIS | PRO | ||||
| 5 | ASP | LEU | ASN | VAL | ARG | GLY | GLY | ALA | SER | PHE | ||||
| 6 | VAL | PRO | SER | GLU | THR | ASN | PRO | TYR | GLN | ASP | ||||
| 7 | GLY | SER | SER | HIS | GLY | THR | HIS | VAL | ALA | GLY | ||||
| 8 | THR | ILE | ALA | ALA | LEU | ASN | ASN | SER | ILE | GLY | ||||
| 9 | VAL | LEU | GLY | VAL | ALA | PRO | SER | ALA | SER | LEU | ||||
| 10 | TYR | ALA | VAL | LYS | VAL | LEU | ASP | SER | THR | GLY | ||||
| 11 | SER | GLY | GLN | TYR | SER | TRP | ILE | ILE | ASN | GLY | ||||
| 12 | ILE | GLU | TRP | ALA | ILE | SER | ASN | ASN | MET | ASP | ||||
| 13 | VAL | ILE | ASN | MET | SER | LEU | GLY | GLY | PRO | THR | ||||
| 14 | GLY | SER | THR | ALA | LEU | LYS | THR | VAL | VAL | ASP | ||||
| 15 | LYS | ALA | VAL | SER | SER | GLY | ILE | VAL | VAL | ALA | ||||
| 16 | ALA | ALA | ALA | GLY | ASN | GLU | GLY | SER | SER | GLY | ||||
| 17 | SER | THR | SER | THR | VAL | GLY | TYR | PRO | ALA | LYS | ||||
| 18 | TYR | PRO | SER | THR | ILE | ALA | VAL | GLY | ALA | VAL | ||||
| 19 | ASN | SER | SER | ASN | GLN | ARG | ALA | SER | PHE | SER | ||||
| 20 | SER | ALA | GLY | SER | GLU | LEU | ASP | VAL | MET | ALA | ||||
| 21 | PRO | GLY | VAL | SER | ILE | GLN | SER | THR | LEU | PRO | ||||
| 22 | GLY | GLY | THR | TYR | GLY | ALA | TYR | ASN | GLY | THR | ||||
| 23 | SER | MET | ALA | THR | PRO | HIS | VAL | ALA | GLY | ALA | ||||
| 24 | ALA | ALA | LEU | ILE | LEU | SER | LYS | HIS | PRO | THR | ||||
| 25 | TRP | THR | ASN | ALA | GLN | VAL | ARG | ASP | ARG | LEU | ||||
| 26 | GLU | SER | THR | ALA | THR | TYR | LEU | GLY | ASN | SER | ||||
| 27 | PHE | TYR | TYR | GLY | LYS | GLY | LEU | ILE | ASN | VAL | ||||
| 28 | GLN | ALA | ALA | ALA | GLN |
Entity 2, DFP - Formula weight is not available
| 1 | DFP |
Entity 3, Calcium - Formula weight is not available
| 1 | CA |
Related Database Links:
| PDB | 1MEE 1SCJ 3VYV 3WHI 4DWW |
| DBJ | BAA00186 BAA04989 BAI84580 BAM49945 BAM57214 |
| EMBL | CAA74536 CAB12870 CAC41625 CAE18180 CCG39575 |
| GB | AAA22247 AAA22742 AAB47045 AAC60424 AAK54130 |
| PRF | 1203267A 1408206A 1903156A |
| REF | NP_388911 WP_003233171 WP_003239459 WP_009966941 WP_010333625 |
| SP | P00783 P04189 P07518 P29142 P35835 |
| AlphaFold | P00783 P04189 P07518 P29142 P35835 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks