BMRB Entry 15722

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15722

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Title:
1H, 13C and 15N Resonance Assignments of Ca2+ bound collagen-binding domain derived from a clostridial collagenase
Deposition date:
2008-04-08
Original release date:
2008-08-08
Authors:
Philominathan, Sagaya T. Leena; Matsushita, Osamu; Jordan, J. Brad; Sakon, Joshua
Citation:

Citation: Philominathan, Sagaya Theresa Leena; Matsushita, Osamu; Jordan, John Brad; Sakon, Joshua. "1H, 13C and 15N resonance assignments of Ca2+ bound collagen-binding domain derived from a clostridial collagenase"  Biomol. NMR Assignments 2, 127-129 (2008).
PubMed: 19636886

Assembly members:

Assembly members:
Collagen_binding_domain_derived_from_Clostridium_Histolyticum, polymer, 122 residues, 13786 Da.
CA, non-polymer, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus histolyticus   Taxonomy ID: 1498   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Clostridium histolyticum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Collagen_binding_domain_derived_from_Clostridium_Histolyticum: GSPGIPGNEKLKEKENNDSS DKATVIPNFNTTMQGSLLGD DSRDYYSFEVKEEGEVNIEL DKKDEFGVTWTLHPESNIND RITYGQVDGNKVSNKVKLRP GKYYLLVYKYSGSGNYELRV NK

Data sets:
Data typeCount
13C chemical shifts420
15N chemical shifts105
1H chemical shifts593

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Collagen binding domain derived from Clostridium Histolyticum1
2Calcium ion, 2+2

Entities:

Entity 1, Collagen binding domain derived from Clostridium Histolyticum 122 residues - 13786 Da.

Residues 887-892 are introduced as clonging artifact

1   GLYSERPROGLYILEPROGLYASNGLULYS
2   LEULYSGLULYSGLUASNASNASPSERSER
3   ASPLYSALATHRVALILEPROASNPHEASN
4   THRTHRMETGLNGLYSERLEULEUGLYASP
5   ASPSERARGASPTYRTYRSERPHEGLUVAL
6   LYSGLUGLUGLYGLUVALASNILEGLULEU
7   ASPLYSLYSASPGLUPHEGLYVALTHRTRP
8   THRLEUHISPROGLUSERASNILEASNASP
9   ARGILETHRTYRGLYGLNVALASPGLYASN
10   LYSVALSERASNLYSVALLYSLEUARGPRO
11   GLYLYSTYRTYRLEULEUVALTYRLYSTYR
12   SERGLYSERGLYASNTYRGLULEUARGVAL
13   ASNLYS

Entity 2, Calcium ion, 2+ - Formula weight is not available

1   CA

Related Database Links:

PDB 1NQD 1NQJ 2O8O 4HPK

Download HSQC peak lists in one of the following formats:
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