BMRB Entry 15755

Name: NMR Solution structure of the C-terminal domain (T94-Y172) of the human centrin 2 in complex with a repeat sequence of human Sfi1 (R641-T660)
Published: 2009-11-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15755

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Solution structure of the C-terminal domain (T94-Y172) of the human centrin 2 in complex with a repeat sequence of human Sfi1 (R641-T660)

Deposition date:

2008-05-06

Original release date:

2009-11-20

Authors:

Martinez Sanz, Juan; Assairi, Liliane; Blouquit, Yves; Duchambon, Patricia; Mouawad, Liliane; Craescu, Constantin T.

Citation:

Citation: Martinez-Sanz, Juan; Kateb, Fatiha; Assairi, Liliane; Blouquit, Yves; Bodenhausen, Geoffrey; Abergel, Daniel; Mouawad, Liliane; Craescu, Constantin. "Structure, dynamics and thermodynamics of the human centrin 2/hSfi1 complex." J. Mol. Biol. 395, 191-204 (2010).
PubMed: 19857500

Assembly members:

Assembly members:
C-HsCen2, polymer, 79 residues, 9234.394 Da.
R17-HsSfi1, polymer, 20 residues, 2381.722 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET24A(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-HsCen2: TQKMSEKDTKEEILKAFKLF DDDETGKISFKNLKRVAKEL GENLTDEELQEMIDEADRDG DGEVSEQEFLRIMKKTSLY
R17-HsSfi1: RADLHHQHSVLHRALQAWVT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	76
15N chemical shifts	75
1H chemical shifts	508

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C-HsCen2	1
2	R17-HsSfi1	2

Entities:

Entity 1, C-HsCen2 79 residues - 9234.394 Da.

1

THR

GLN

LYS

MET

SER

GLU

LYS

ASP

THR

LYS

2

GLU

ILE

LEU

LYS

ALA

PHE

LYS

LEU

PHE

3

ASP

GLU

THR

GLY

LYS

ILE

SER

PHE

4

LYS

ASN

LEU

LYS

ARG

VAL

ALA

LYS

GLU

LEU

5

GLY

GLU

ASN

LEU

THR

ASP

GLU

LEU

GLN

6

GLU

MET

ILE

ASP

GLU

ALA

ASP

ARG

ASP

GLY

7

ASP

GLY

GLU

VAL

SER

GLU

GLN

GLU

PHE

LEU

8

ARG

ILE

MET

LYS

THR

SER

LEU

TYR

Entity 2, R17-HsSfi1 20 residues - 2381.722 Da.

1		ARG	ALA	ASP	LEU	HIS	HIS	GLN	HIS	SER	VAL
2		LEU	HIS	ARG	ALA	LEU	GLN	ALA	TRP	VAL	THR

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_3	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_3	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_3	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HN(CO)CA	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_4	isotropic	sample_conditions_1

PDB	1M39 2A4J 2K2I 2K2I
GB	EPY78459 ERE65656 AAH21576 AAI10815 AAI29927 AAI29946 EAW59980
DBJ	BAA25468 BAF85097 BAG11202 BAH11642 BAH13949
REF	NP_001007468 NP_001245254 NP_001245255 NP_001245256 NP_055590
SP	A8K8P3
AlphaFold	A8K8P3

BMRB Entry 15755

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1		ARG	ALA	ASP	LEU	HIS	HIS	GLN	HIS	SER	VAL
2		LEU	HIS	ARG	ALA	LEU	GLN	ALA	TRP	VAL	THR

1		ARG	ALA	ASP	LEU	HIS	HIS	GLN	HIS	SER	VAL
2		LEU	HIS	ARG	ALA	LEU	GLN	ALA	TRP	VAL	THR

1		ARG	ALA	ASP	LEU	HIS	HIS	GLN	HIS	SER	VAL
2		LEU	HIS	ARG	ALA	LEU	GLN	ALA	TRP	VAL	THR