BMRB Entry 15945

Chem Shift validation: LACS
BMRB Entry DOI: doi:10.13018/BMR15945

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Title:
MDM2 N-terminal domain
Deposition date:
2008-09-08
Original release date:
2008-10-13
Authors:
Riedinger, Christiane; Mcdonnell, James
Citation:

Citation: Riedinger, Christiane; Endicott, Jane; Kemp, Stuart; Smyth, Lynette; Watson, Anna; Valeur, Eric; Golding, Bernard; Griffin, Roger; Hardcastle, Ian; Noble, Martin; McDonnell, James. "Analysis of chemical shift changes reveals the binding modes of isoindolinone inhibitors of the MDM2-p53 interaction"  J. Am. Chem. Soc. 130, 16038-16044 (2008).
PubMed: 18959403

Assembly members:

Assembly members:
human_mdm2_N-terminal_domain, polymer, 114 residues, 12931.8 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX 6P1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human_mdm2_N-terminal_domain: GPLGSSQIPASEQETLVRPK PLLLKLLKSVGAQKDTYTMK EVLFYLGQYIMTKRLYDEKQ QHIVYCSNDLLGDLFGVPSF SVKEHRKIYTMIYRNLVVVN QQESSDSGTSVSEN

Data sets:
Data typeCount
13C chemical shifts203
15N chemical shifts101
1H chemical shifts193

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminal domain, p53 binding domain1

Entities:

Entity 1, N-terminal domain, p53 binding domain 114 residues - 12931.8 Da.

residues 12-16 correspond to the remnant of a GST-tag

1   GLYPROLEUGLYSERSERGLNILEPROALA
2   SERGLUGLNGLUTHRLEUVALARGPROLYS
3   PROLEULEULEULYSLEULEULYSSERVAL
4   GLYALAGLNLYSASPTHRTYRTHRMETLYS
5   GLUVALLEUPHETYRLEUGLYGLNTYRILE
6   METTHRLYSARGLEUTYRASPGLULYSGLN
7   GLNHISILEVALTYRCYSSERASNASPLEU
8   LEUGLYASPLEUPHEGLYVALPROSERPHE
9   SERVALLYSGLUHISARGLYSILETYRTHR
10   METILETYRARGASNLEUVALVALVALASN
11   GLNGLNGLUSERSERASPSERGLYTHRSER
12   VALSERGLUASN

Related Database Links:

PDB 1RV1 1T4E 1T4F 1YCR 2AXI 2GV2 2M86 3EQS 3G03 3IUX 3IWY 3JZK 3JZR 3JZS 3LBK 3LBL 3LNJ 3LNZ 3TJ2 3TPX 3TU1 3V3B 3VBG 3VZV 3W69 4DIJ 4ERE 4ERF 4HFZ 4HG7 4JV7 4JV9 4JVE 4JVR 4JWR 4MDN 4MDQ 4OAS 4OBA 4OCC 4OGV 4OQ3 4QO4 4QOC 4ZYC 4ZYF 4ZYI
REF XP_006719462 XP_012911529

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