BMRB Entry 15965

Click here to enlarge.

PDB ID: 2k8x
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15965
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Structural Transitions of the N Terminus of a Short Non-Muscle Tropomyosin upon Binding to the C Terminus in Solution
Deposition date:
2008-09-25
Original release date:
2009-04-02
Authors:
Greenfield, Norma; Kotylanskaya, Lucy; Hitchcock-DeGregori, Sarah
Citation:

Citation: Greenfield, Norma; Kotlyanskaya, Lucy; Hitchcock-DeGregori, Sarah. "Structure of the N Terminus of a Nonmuscle alpha-Tropomyosin in Complex with the C Terminus: Implications for Actin Binding"  Biochemistry 48, 1272-1283 (2009).
PubMed: 19170537

Assembly members:

Assembly members:
TM1b(1-19)Zip, polymer, 38 residues, 4331.956 Da.
TM9d(252-284), polymer, 37 residues, 4115.06 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pPROEX HTa

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TM1b(1-19)Zip: GAGSSSLEAVRRKIRSLQEQ NYHLENEVARLKKLVGER
TM9d(252-284): GCGGSIDDLEEKVAHAKEEN LSMHQMLDQTLLELNNM

Data sets:
Data typeCount
13C chemical shifts123
15N chemical shifts41
1H chemical shifts273

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_22
2entity_11

Entities:

Entity 2, entity_2 37 residues - 4115.06 Da.

Residues 252-284 are encoded by exon 8 and 9d or rat alpha tropomyosin (TPM1 gene). Residues 248-251, GCGG were added to allow disulfide cross linking.

1   GLYCYSGLYGLYSERILEASPASPLEUGLU
2   GLULYSVALALAHISALALYSGLUGLUASN
3   LEUSERMETHISGLNMETLEUASPGLNTHR
4   LEULEUGLULEUASNASNMET

Entity 1, entity_1 38 residues - 4331.956 Da.

Residue 0 is a glycine group replacing a native acetyl group residue 1-19 are the first 19 residues of a rat non-muscle low molecular weight tropomyosin encoded by exon 1b (TPM1 gene). 0 is a glycine residue that substitutes for the native acetyl group. Residues 20-37 are the last 18 residues of the yeast transcription factor GCN4.

1   GLYALAGLYSERSERSERLEUGLUALAVAL
2   ARGARGLYSILEARGSERLEUGLNGLUGLN
3   ASNTYRHISLEUGLUASNGLUVALALAARG
4   LEULYSLYSLEUVALGLYGLUARG

Related Database Links:

PDB 1IHQ 2K8X 3AZD 3MUD
DBJ BAD92278 BAG11063 BAH12832 BAH13458 BAB22620 BAD18535 BAE88136 BAH13458
GB AAA18098 AAA18099 AAA42253 AAH50473 AAH53545 AAA18097 AAA18098 AAA18099 AAA36771 AAA40483
REF NP_001018008 NP_001029245 NP_001029246 NP_001029247 NP_001157724 NP_001018004 NP_001018006 NP_001018007 NP_001018020 NP_001029241
EMBL CAA03930 CAA04505 CAA26258 CAA30930 CAB43309
PIR A22165
PRF 1105305A

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks