BMRB Entry 15991

Name: Structure of the Core Binding Domain of sigma54
Published: 2009-05-29

Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15991

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of the Core Binding Domain of sigma54

Deposition date:

2008-10-19

Original release date:

2009-05-29

Authors:

Hong, Eunmi; Wemmer, David E.

Citation:

Citation: Hong, Eunmi; Douclaff, Michaeleen; Wemmer, David. "Structure of the RNA Polymerase Core-Binding Domain of sigma(54) Reveals a Likely Conformational Fracture Point." J. Mol. Biol. 390, 70-82 (2009).
PubMed: 19426742

Assembly members:

Assembly members:
Core_Binding_Domain, polymer, 130 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Aquifex aeolicus Taxonomy ID: 63363 Superkingdom: Bacteria Kingdom: not available Genus/species: Aquifex aeolicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21b3-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Core_Binding_Domain: YTPSELEELQQNIKLELEGK EQELALELLNYLNEKGFLSK SVEEISDVLRCSVEELEKVR QKVLRLEPLGVCSKDVWEFL ELQIEEIYPEEEEILKKALR DLKRGKKLKPEIKGKLSRLR LFPLSSSAEK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	419
15N chemical shifts	126
1H chemical shifts	975

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit 1	1

Entities:

Entity 1, subunit 1 130 residues - Formula weight is not available

1	TYR	THR	PRO	SER	GLU	LEU	GLU	GLU	LEU	GLN
2	GLN	ASN	ILE	LYS	LEU	GLU	LEU	GLU	GLY	LYS
3	GLU	GLN	GLU	LEU	ALA	LEU	GLU	LEU	LEU	ASN
4	TYR	LEU	ASN	GLU	LYS	GLY	PHE	LEU	SER	LYS
5	SER	VAL	GLU	GLU	ILE	SER	ASP	VAL	LEU	ARG
6	CYS	SER	VAL	GLU	GLU	LEU	GLU	LYS	VAL	ARG
7	GLN	LYS	VAL	LEU	ARG	LEU	GLU	PRO	LEU	GLY
8	VAL	CYS	SER	LYS	ASP	VAL	TRP	GLU	PHE	LEU
9	GLU	LEU	GLN	ILE	GLU	GLU	ILE	TYR	PRO	GLU
10	GLU	GLU	GLU	ILE	LEU	LYS	LYS	ALA	LEU	ARG
11	ASP	LEU	LYS	ARG	GLY	LYS	LYS	LEU	LYS	PRO
12	GLU	ILE	LYS	GLY	LYS	LEU	SER	ARG	LEU	ARG
13	LEU	PHE	PRO	LEU	SER	SER	SER	ALA	GLU	LYS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1

BMRB	15990
PDB	2K9L 2K9M
GB	AAC06814
REF	NP_213418 WP_010880356

BMRB Entry 15991

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	TYR	THR	PRO	SER	GLU	LEU	GLU	GLU	LEU	GLN
2	GLN	ASN	ILE	LYS	LEU	GLU	LEU	GLU	GLY	LYS
3	GLU	GLN	GLU	LEU	ALA	LEU	GLU	LEU	LEU	ASN
4	TYR	LEU	ASN	GLU	LYS	GLY	PHE	LEU	SER	LYS
5	SER	VAL	GLU	GLU	ILE	SER	ASP	VAL	LEU	ARG
6	CYS	SER	VAL	GLU	GLU	LEU	GLU	LYS	VAL	ARG
7	GLN	LYS	VAL	LEU	ARG	LEU	GLU	PRO	LEU	GLY
8	VAL	CYS	SER	LYS	ASP	VAL	TRP	GLU	PHE	LEU
9	GLU	LEU	GLN	ILE	GLU	GLU	ILE	TYR	PRO	GLU
10	GLU	GLU	GLU	ILE	LEU	LYS	LYS	ALA	LEU	ARG
11	ASP	LEU	LYS	ARG	GLY	LYS	LYS	LEU	LYS	PRO
12	GLU	ILE	LYS	GLY	LYS	LEU	SER	ARG	LEU	ARG
13	LEU	PHE	PRO	LEU	SER	SER	SER	ALA	GLU	LYS

1	TYR	THR	PRO	SER	GLU	LEU	GLU	GLU	LEU	GLN
2	GLN	ASN	ILE	LYS	LEU	GLU	LEU	GLU	GLY	LYS
3	GLU	GLN	GLU	LEU	ALA	LEU	GLU	LEU	LEU	ASN
4	TYR	LEU	ASN	GLU	LYS	GLY	PHE	LEU	SER	LYS
5	SER	VAL	GLU	GLU	ILE	SER	ASP	VAL	LEU	ARG
6	CYS	SER	VAL	GLU	GLU	LEU	GLU	LYS	VAL	ARG
7	GLN	LYS	VAL	LEU	ARG	LEU	GLU	PRO	LEU	GLY
8	VAL	CYS	SER	LYS	ASP	VAL	TRP	GLU	PHE	LEU
9	GLU	LEU	GLN	ILE	GLU	GLU	ILE	TYR	PRO	GLU
10	GLU	GLU	GLU	ILE	LEU	LYS	LYS	ALA	LEU	ARG
11	ASP	LEU	LYS	ARG	GLY	LYS	LYS	LEU	LYS	PRO
12	GLU	ILE	LYS	GLY	LYS	LEU	SER	ARG	LEU	ARG
13	LEU	PHE	PRO	LEU	SER	SER	SER	ALA	GLU	LYS

1	TYR	THR	PRO	SER	GLU	LEU	GLU	GLU	LEU	GLN
2	GLN	ASN	ILE	LYS	LEU	GLU	LEU	GLU	GLY	LYS
3	GLU	GLN	GLU	LEU	ALA	LEU	GLU	LEU	LEU	ASN
4	TYR	LEU	ASN	GLU	LYS	GLY	PHE	LEU	SER	LYS
5	SER	VAL	GLU	GLU	ILE	SER	ASP	VAL	LEU	ARG
6	CYS	SER	VAL	GLU	GLU	LEU	GLU	LYS	VAL	ARG
7	GLN	LYS	VAL	LEU	ARG	LEU	GLU	PRO	LEU	GLY
8	VAL	CYS	SER	LYS	ASP	VAL	TRP	GLU	PHE	LEU
9	GLU	LEU	GLN	ILE	GLU	GLU	ILE	TYR	PRO	GLU
10	GLU	GLU	GLU	ILE	LEU	LYS	LYS	ALA	LEU	ARG
11	ASP	LEU	LYS	ARG	GLY	LYS	LYS	LEU	LYS	PRO
12	GLU	ILE	LYS	GLY	LYS	LEU	SER	ARG	LEU	ARG
13	LEU	PHE	PRO	LEU	SER	SER	SER	ALA	GLU	LYS