BMRB Entry 16176
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PDB ID: 2kfd
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16176
MolProbity Validation Chart
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Citation: Bonet, Roman; Ruiz, Lidia; Morales, Begona; Macias, Maria. "Solution structure of the fourth FF domain of yeast Prp40 splicing factor." Proteins 77, 1000-1003 (2009).
PubMed: 19722265
Assembly members:
yeast_Prp40_FF4_domain, polymer, 69 residues, 7900.010 Da.
Natural source: Common Name: yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM30
Entity Sequences (FASTA):
yeast_Prp40_FF4_domain: GAMGNERRILEQKKHYFWLL
LQRTYTKTGKPKPSTWDLAS
KELGESLEYKALGDEDNIRR
QIFEDFKPE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 249 |
| 15N chemical shifts | 61 |
| 1H chemical shifts | 497 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | yeast_Prp40_FF4_domain | 1 |
Entities:
Entity 1, yeast_Prp40_FF4_domain 69 residues - 7900.010 Da.
FF4 domain correspond to residues 488-552 of Prp40.Residues 1-4 in the chem.shift list remain from the cloning after cleavage of the tag
| 1 | GLY | ALA | MET | GLY | ASN | GLU | ARG | ARG | ILE | LEU | ||||
| 2 | GLU | GLN | LYS | LYS | HIS | TYR | PHE | TRP | LEU | LEU | ||||
| 3 | LEU | GLN | ARG | THR | TYR | THR | LYS | THR | GLY | LYS | ||||
| 4 | PRO | LYS | PRO | SER | THR | TRP | ASP | LEU | ALA | SER | ||||
| 5 | LYS | GLU | LEU | GLY | GLU | SER | LEU | GLU | TYR | LYS | ||||
| 6 | ALA | LEU | GLY | ASP | GLU | ASP | ASN | ILE | ARG | ARG | ||||
| 7 | GLN | ILE | PHE | GLU | ASP | PHE | LYS | PRO | GLU |
Related Database Links:
| PDB | 2KFD |
| DBJ | GAA24712 |
| EMBL | CAA81847 CAY81071 |
| GB | AAB24902 AHY76231 AJP40025 AJS30223 AJS30524 |
| REF | NP_012913 |
| SP | P33203 |
| TPG | DAA09144 |
| AlphaFold | P33203 |
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