BMRB Entry 16321

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PDB ID: 2kjh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16321
MolProbity Validation Chart

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Title:
Backbone 1H, 13C and 15N resonance assignments for the E2 conjugating enzyme, UbcH8
Deposition date:
2009-05-28
Original release date:
2009-10-14
Authors:
Serniwka, Stephanie; Shaw, Gary
Citation:

Citation: Serniwka, Stephanie; Shaw, Gary. "The structure of the UbcH8-ubiquitin complex shows a unique ubiquitin interaction site"  Biochemistry 48, 12169-12179 (2009).
PubMed: 19928833

Assembly members:

Assembly members:
UbcH8, polymer, 152 residues, 17638 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UbcH8: MASMRVVKELEDLQKKPPPY LRNLSSDDANVLVWHALLLP DQPPYHLKAFNLRISFPPEY PFKPPMIKFTTKIYHPNVDE NGQICLPIISSENWKPSTKT SQVLEALNVLVNRPNIREPL RMDLADLLTQNPELFRKNAE EFTLRFGVDRPS

Data sets:
Data typeCount
13C chemical shifts460
15N chemical shifts140
1H chemical shifts446

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
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Assembly:

Entity Assembly IDEntity NameEntity ID
1UbcH81

Entities:

Entity 1, UbcH8 152 residues - 17638 Da.

1   METALASERMETARGVALVALLYSGLULEU
2   GLUASPLEUGLNLYSLYSPROPROPROTYR
3   LEUARGASNLEUSERSERASPASPALAASN
4   VALLEUVALTRPHISALALEULEULEUPRO
5   ASPGLNPROPROTYRHISLEULYSALAPHE
6   ASNLEUARGILESERPHEPROPROGLUTYR
7   PROPHELYSPROPROMETILELYSPHETHR
8   THRLYSILETYRHISPROASNVALASPGLU
9   ASNGLYGLNILECYSLEUPROILEILESER
10   SERGLUASNTRPLYSPROSERTHRLYSTHR
11   SERGLNVALLEUGLUALALEUASNVALLEU
12   VALASNARGPROASNILEARGGLUPROLEU
13   ARGMETASPLEUALAASPLEULEUTHRGLN
14   ASNPROGLULEUPHEARGLYSASNALAGLU
15   GLUPHETHRLEUARGPHEGLYVALASPARG
16   PROSER

Related Database Links:

SWS O14933
PDB 1WZV 1WZW 2KJH
DBJ BAG38109
EMBL CAB64566 CAG33407
GB AAB86433 AAD17525 AAH32491 ABM82217 ABM85401
REF NP_004214 NP_937826 XP_003318029 XP_003826475 XP_004051215
SP O14933
AlphaFold O14933

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks