BMRB Entry 16355

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PDB ID: 2kk8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16355
MolProbity Validation Chart

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Title:
NMR Solution Structure of a Putative Uncharacterized Protein obtained from Arabidopsis thaliana: Northeast Structural Genomics Consortium target AR3449A
Deposition date:
2009-06-16
Original release date:
2012-02-22
Authors:
Mani, Rajeswari; Gurla, Swapna; Shastry, Ritu; Ciccosanti, Colleen; Foote, Erica; Jiang, Mei; Xiao, Rong; Nair, Rajesh; Everett, John; Huang, Yuanpeng; Acton, Tom; Rost, Burkhard; Montelione, Gaetano
Citation:

Citation: Mani, Rajeswari; Gurla, Swapna; Shastry, Ritu; Ciccosanti, Colleen; Foote, Erica; Jiang, Mei; Xiao, Rong; Nair, Rajesh; Everett, John; Huang, Yuanpeng; Acton, Tom; Rost, Burkhard; Montelione, Gaetano. "NMR Solution Structure of a Putative Uncharacterized Protein obtained from Arabidopsis thaliana: Northeast Structural Genomics Consortium Target AR3449A"  .

Assembly members:

Assembly members:
AR3449A, polymer, 84 residues, 9679.103 Da.

Natural source:

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21-23C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AR3449A: MGHHHHHHSHMKFLVENLNG SSFELEVDYRDTLLVVKQKI ERSQHIPVSKQTLIVDGIVI LREDLTVEQCQIVPTSDIQL EVSS

Data sets:
Data typeCount
13C chemical shifts342
15N chemical shifts81
1H chemical shifts552

Time Domain Data

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1AR3449A1

Entities:

Entity 1, AR3449A 84 residues - 9679.103 Da.

1-10 residues MGHHHHHHSH is a N-terminal tag

1   METGLYHISHISHISHISHISHISSERHIS
2   METLYSPHELEUVALGLUASNLEUASNGLY
3   SERSERPHEGLULEUGLUVALASPTYRARG
4   ASPTHRLEULEUVALVALLYSGLNLYSILE
5   GLUARGSERGLNHISILEPROVALSERLYS
6   GLNTHRLEUILEVALASPGLYILEVALILE
7   LEUARGGLUASPLEUTHRVALGLUGLNCYS
8   GLNILEVALPROTHRSERASPILEGLNLEU
9   GLUVALSERSER

Related Database Links:

PDB 2KK8
EMBL CAB81069
GB AEE82498
REF NP_192436

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