BMRB Entry 16362

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16362

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Title:
1H, 13C and 15N NMR assignments of StnII-R29Q, a defective lipid binding mutant of the sea anemone actinoporin Sticholysin II.
Deposition date:
2009-06-24
Original release date:
2009-11-16
Authors:
Castrillo, Ines; Bruix, Marta; Santoro, Jorge
Citation:

Citation: Castrillo, Ines; Alegre-Cebollada, Jorge; Martinez-Del-Pozo, Alvaro; Gavilanes, Jose; Bruix, Marta. "(1)H, (13)C, and (15)N NMR assignments of StnII-R29Q, a defective lipid binding mutant of the sea anemone actinoporin Sticholysin II."  Biomol NMR Assign 3, 239-241 (2009).
PubMed: 19768581

Assembly members:

Assembly members:
R29Q_StnII, polymer, 175 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE60

Entity Sequences (FASTA):

Entity Sequences (FASTA):
R29Q_StnII: ALAGTIIAGASLTFQVLDKV LEELGKVSQKIAVGIDNESG GTWTALNAYFRSGTTDVILP EFVPNTKALLYSGRKDTGPV ATGAVAAFAYYMSSGNTLGV MFSVPFDYNWYSNWWDVKIY SGKRRADQGMYEDLYYGNPY RGDNGWHEKNLGYGLRMKGI MTSAGEAKMQIKISR

Data sets:
Data typeCount
13C chemical shifts412
15N chemical shifts178
1H chemical shifts1142

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer1

Entities:

Entity 1, monomer 175 residues - Formula weight is not available

1   ALALEUALAGLYTHRILEILEALAGLYALA
2   SERLEUTHRPHEGLNVALLEUASPLYSVAL
3   LEUGLUGLULEUGLYLYSVALSERGLNLYS
4   ILEALAVALGLYILEASPASNGLUSERGLY
5   GLYTHRTRPTHRALALEUASNALATYRPHE
6   ARGSERGLYTHRTHRASPVALILELEUPRO
7   GLUPHEVALPROASNTHRLYSALALEULEU
8   TYRSERGLYARGLYSASPTHRGLYPROVAL
9   ALATHRGLYALAVALALAALAPHEALATYR
10   TYRMETSERSERGLYASNTHRLEUGLYVAL
11   METPHESERVALPROPHEASPTYRASNTRP
12   TYRSERASNTRPTRPASPVALLYSILETYR
13   SERGLYLYSARGARGALAASPGLNGLYMET
14   TYRGLUASPLEUTYRTYRGLYASNPROTYR
15   ARGGLYASPASNGLYTRPHISGLULYSASN
16   LEUGLYTYRGLYLEUARGMETLYSGLYILE
17   METTHRSERALAGLYGLUALALYSMETGLN
18   ILELYSILESERARG

Related Database Links:

BMRB 16630
PDB 1GWY 1O71 1O72 2L2B 2L38
EMBL CAC20912
SP P07845
AlphaFold P07845

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SPARKY: Backbone or all simulated peaks