BMRB Entry 16422

Name: Solution Structure and Dynamics of the N-terminal Cystosolic Domain of Rhomboid Intramembrane Protease from Pseudomonas aeruginosa: Insights into a Functional Role in Intramembrane Proteolysis
Published: 2010-03-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16422

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure and Dynamics of the N-terminal Cystosolic Domain of Rhomboid Intramembrane Protease from Pseudomonas aeruginosa: Insights into a Functional Role in Intramembrane Proteolysis

Deposition date:

2009-07-23

Original release date:

2010-03-08

Authors:

Del Rio, Armando; Dutta, Kaushik; Chavez, Jose; Ubarretxena-Belandia, Iban; Ghose, Ranajeet

Citation:

Citation: Del Rio, Armando; Dutta, Kaushik; Chavez, Jose; Ubarretxena-Belandia, Iban; Ghose, Ranajeet. "Solution Structure and Dynamics of the N-terminal Cystosolic Domain of Rhomboid Intramembrane Protease from Pseudomonas aeruginosa: Insights into a Functional Role in Intramembrane Proteolysis" J. Mol. Biol. 1, 109-122 (2007).
PubMed: 17059825

Assembly members:

Assembly members:
NRho, polymer, 70 residues, 407.568 Da.
C16PN, non-polymer, 407.568 Da.

Natural source:

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NRho: MSAVQVLKFPLSVDLAGFVG LLRRLNVPHRVSEESGQQVL WVPDERLAEQVRELYRRYPE GDPQATLEAA

Data sets:

binding_constants

Data type	Count
binding constants	8

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NRho	1
2	C16PN	2

Entities:

Entity 1, NRho 70 residues - 407.568 Da.

1	MET	SER	ALA	VAL	GLN	VAL	LEU	LYS	PHE	PRO
2	LEU	SER	VAL	ASP	LEU	ALA	GLY	PHE	VAL	GLY
3	LEU	LEU	ARG	ARG	LEU	ASN	VAL	PRO	HIS	ARG
4	VAL	SER	GLU	GLU	SER	GLY	GLN	GLN	VAL	LEU
5	TRP	VAL	PRO	ASP	GLU	ARG	LEU	ALA	GLU	GLN
6	VAL	ARG	GLU	LEU	TYR	ARG	ARG	TYR	PRO	GLU
7	GLY	ASP	PRO	GLN	ALA	THR	LEU	GLU	ALA	ALA

Entity 2, C16PN - C16PN - 407.568 Da.

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_4	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_5	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_6	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_7	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_8	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_9	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_10	isotropic	sample_conditions_1

PDB	2GQC
DBJ	BAK91039 BAP22307 BAP49934 BAQ38821 BAR66878
EMBL	CAW26702 CCQ87103 CDH70226 CDH76316 CDI91489
GB	AAG06474 ABJ12318 ABR81084 AEO74363 AFM64074
REF	NP_251776 WP_003091333 WP_003104987 WP_003110073 WP_003138531

BMRB Entry 16422

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	SER	ALA	VAL	GLN	VAL	LEU	LYS	PHE	PRO
2	LEU	SER	VAL	ASP	LEU	ALA	GLY	PHE	VAL	GLY
3	LEU	LEU	ARG	ARG	LEU	ASN	VAL	PRO	HIS	ARG
4	VAL	SER	GLU	GLU	SER	GLY	GLN	GLN	VAL	LEU
5	TRP	VAL	PRO	ASP	GLU	ARG	LEU	ALA	GLU	GLN
6	VAL	ARG	GLU	LEU	TYR	ARG	ARG	TYR	PRO	GLU
7	GLY	ASP	PRO	GLN	ALA	THR	LEU	GLU	ALA	ALA

1	MET	SER	ALA	VAL	GLN	VAL	LEU	LYS	PHE	PRO
2	LEU	SER	VAL	ASP	LEU	ALA	GLY	PHE	VAL	GLY
3	LEU	LEU	ARG	ARG	LEU	ASN	VAL	PRO	HIS	ARG
4	VAL	SER	GLU	GLU	SER	GLY	GLN	GLN	VAL	LEU
5	TRP	VAL	PRO	ASP	GLU	ARG	LEU	ALA	GLU	GLN
6	VAL	ARG	GLU	LEU	TYR	ARG	ARG	TYR	PRO	GLU
7	GLY	ASP	PRO	GLN	ALA	THR	LEU	GLU	ALA	ALA

1	MET	SER	ALA	VAL	GLN	VAL	LEU	LYS	PHE	PRO
2	LEU	SER	VAL	ASP	LEU	ALA	GLY	PHE	VAL	GLY
3	LEU	LEU	ARG	ARG	LEU	ASN	VAL	PRO	HIS	ARG
4	VAL	SER	GLU	GLU	SER	GLY	GLN	GLN	VAL	LEU
5	TRP	VAL	PRO	ASP	GLU	ARG	LEU	ALA	GLU	GLN
6	VAL	ARG	GLU	LEU	TYR	ARG	ARG	TYR	PRO	GLU
7	GLY	ASP	PRO	GLN	ALA	THR	LEU	GLU	ALA	ALA