BMRB Entry 16430

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16430

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Title:
1H, 13C, 15N backbone resonance assginments of Nep1 from Methanococcus jannaschii
Deposition date:
2009-07-29
Original release date:
2009-09-08
Authors:
Wurm, Philip; Duchardt, Elke; Meyer, Britta; Leal, Belinda; Koetter, Peter; Entian, Karl-Dieter
Citation:

Citation: Wurm, Philip; Duchardt, Elke; Meyer, Britta; Leal, Belinda; Koetter, Peter; Entian, Karl-Dieter; Woehnert, Jens. "Backbone resonance assignments of the 48 kDa dimeric putative rRNA-methyltransferase Nep1 from Methanocaldococcus jannaschii"  Biomol. NMR Assignments 3, 251-254 (2009).
PubMed: 19779849

Assembly members:

Assembly members:
Nep1_chain, polymer, 205 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Methanococcus jannaschii   Taxonomy ID: 2190   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanococcus jannaschii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nep1_chain: MTYNIILAKSALELIPEEIK NKIRKSRVYKYDILDSNYHY KAMEKLKDKEMRGRPDIIHI SLLNILDSPINHEKKLNIYI HTYDDKVLKINPETRLPRNY FRFLGVMEKVLKGERNHLIK MEEKTLEDLLNEINAKKIAI MTKTGKLTHPKLLKEYDTFI IGGFPYGKLKINKEKVFGDI KEISIYNKGLMAWTVCGIIC YSLSF

Data sets:
Data typeCount
13C chemical shifts1198
15N chemical shifts390
1H chemical shifts390

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer 11
2monomer 21

Entities:

Entity 1, monomer 1 205 residues - Formula weight is not available

1   METTHRTYRASNILEILELEUALALYSSER
2   ALALEUGLULEUILEPROGLUGLUILELYS
3   ASNLYSILEARGLYSSERARGVALTYRLYS
4   TYRASPILELEUASPSERASNTYRHISTYR
5   LYSALAMETGLULYSLEULYSASPLYSGLU
6   METARGGLYARGPROASPILEILEHISILE
7   SERLEULEUASNILELEUASPSERPROILE
8   ASNHISGLULYSLYSLEUASNILETYRILE
9   HISTHRTYRASPASPLYSVALLEULYSILE
10   ASNPROGLUTHRARGLEUPROARGASNTYR
11   PHEARGPHELEUGLYVALMETGLULYSVAL
12   LEULYSGLYGLUARGASNHISLEUILELYS
13   METGLUGLULYSTHRLEUGLUASPLEULEU
14   ASNGLUILEASNALALYSLYSILEALAILE
15   METTHRLYSTHRGLYLYSLEUTHRHISPRO
16   LYSLEULEULYSGLUTYRASPTHRPHEILE
17   ILEGLYGLYPHEPROTYRGLYLYSLEULYS
18   ILEASNLYSGLULYSVALPHEGLYASPILE
19   LYSGLUILESERILETYRASNLYSGLYLEU
20   METALATRPTHRVALCYSGLYILEILECYS
21   TYRSERLEUSERPHE

Related Database Links:

PDB 3BBD 3BBE 3BBH
GB AAB98551
SP Q57977
AlphaFold Q57977

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks