BMRB Entry 16470
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16470
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Citation: Smith, Matthew; Hardy, W. Rod; Li, Guang-Yao; Goudreault, Marilyn; Hersch, Steven; Metalnikov, Pavel; Starostine, Andrei; Pawson, Tony; Ikura, Mitsuhiko. "The PTB domain of ShcA couples receptor activation to the cytoskeletal regulator IQGAP1." EMBO J. 29, 884-896 (2010).
PubMed: 20075861
Assembly members:
PTP-PEST_peptide, polymer, 14 residues, 1665.7 Da.
ShcA_PTB_domain, polymer, 191 residues, 20969 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b
Entity Sequences (FASTA):
PTP-PEST_peptide: PLSFTNPLHSDDWH
ShcA_PTB_domain: GQLGGEEWTRHGSFVNKPTR
GWLHPNDKVMGPGVSYLVRY
MGCVEVLQSMRALDFNTRTQ
VTREAISLVCEAVPGAKGAT
RRRKPCSRPLSSILGRSNLK
FAGMPITLTVSTSSLNLMAA
DCKQIIANHHMQSISFASGG
DPDTAEYVAYVAKDPVNQRA
CHILECPEGLAQDVISTIGQ
AFELRFKQYLR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 264 |
| 15N chemical shifts | 153 |
| 1H chemical shifts | 153 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | peptide ligand | 1 |
| 2 | binding domain | 2 |
Entities:
Entity 1, peptide ligand 14 residues - 1665.7 Da.
| 1 | PRO | LEU | SER | PHE | THR | ASN | PRO | LEU | HIS | SER | ||||
| 2 | ASP | ASP | TRP | HIS |
Entity 2, binding domain 191 residues - 20969 Da.
| 1 | GLY | GLN | LEU | GLY | GLY | GLU | GLU | TRP | THR | ARG | ||||
| 2 | HIS | GLY | SER | PHE | VAL | ASN | LYS | PRO | THR | ARG | ||||
| 3 | GLY | TRP | LEU | HIS | PRO | ASN | ASP | LYS | VAL | MET | ||||
| 4 | GLY | PRO | GLY | VAL | SER | TYR | LEU | VAL | ARG | TYR | ||||
| 5 | MET | GLY | CYS | VAL | GLU | VAL | LEU | GLN | SER | MET | ||||
| 6 | ARG | ALA | LEU | ASP | PHE | ASN | THR | ARG | THR | GLN | ||||
| 7 | VAL | THR | ARG | GLU | ALA | ILE | SER | LEU | VAL | CYS | ||||
| 8 | GLU | ALA | VAL | PRO | GLY | ALA | LYS | GLY | ALA | THR | ||||
| 9 | ARG | ARG | ARG | LYS | PRO | CYS | SER | ARG | PRO | LEU | ||||
| 10 | SER | SER | ILE | LEU | GLY | ARG | SER | ASN | LEU | LYS | ||||
| 11 | PHE | ALA | GLY | MET | PRO | ILE | THR | LEU | THR | VAL | ||||
| 12 | SER | THR | SER | SER | LEU | ASN | LEU | MET | ALA | ALA | ||||
| 13 | ASP | CYS | LYS | GLN | ILE | ILE | ALA | ASN | HIS | HIS | ||||
| 14 | MET | GLN | SER | ILE | SER | PHE | ALA | SER | GLY | GLY | ||||
| 15 | ASP | PRO | ASP | THR | ALA | GLU | TYR | VAL | ALA | TYR | ||||
| 16 | VAL | ALA | LYS | ASP | PRO | VAL | ASN | GLN | ARG | ALA | ||||
| 17 | CYS | HIS | ILE | LEU | GLU | CYS | PRO | GLU | GLY | LEU | ||||
| 18 | ALA | GLN | ASP | VAL | ILE | SER | THR | ILE | GLY | GLN | ||||
| 19 | ALA | PHE | GLU | LEU | ARG | PHE | LYS | GLN | TYR | LEU | ||||
| 20 | ARG |
Related Database Links:
| SWS | P35831 |
| BMRB | 17080 5566 |
| PDB | 1N3H 1OY2 1SHC 2L1C |
| DBJ | BAA74950 BAC33706 BAD92086 BAE33083 BAF84832 |
| EMBL | CAA48251 CAA70977 CAH92143 |
| GB | AAA91777 AAB49972 AAC52146 AAH14158 AAH33925 |
| REF | NP_001068773 NP_001106802 NP_001123512 NP_001123513 NP_001126253 |
| SP | P29353 P98083 Q0IIE2 Q5M824 Q5R7W7 |
| TPG | DAA31779 DAA31780 |
| AlphaFold | P29353 P98083 Q0IIE2 Q5M824 Q5R7W7 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks