BMRB Entry 16588
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16588
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Citation: Retzlaff, Marco; Hagn, Franz; Mitschke, Lars; Hessling, Martin; Gugel, Frederik; Kessler, Horst; Richter, Klaus; Buchner, Johannes. "Asymmetric Activation of the Hsp90 Dimer by Its Cochaperone Aha1." Mol. Cell 37, 344-354 (2010).
PubMed: 20159554
Assembly members:
Aha1, polymer, 198 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 385 |
| 15N chemical shifts | 122 |
| 1H chemical shifts | 122 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Aha1 C-terminal domain | 1 |
Entities:
Entity 1, Aha1 C-terminal domain 198 residues - Formula weight is not available
Resiudes 1-4 represent an artificial peptide sequence after His6 tag removal, residues 5-198 represent Aha1 residues 157-350
| 1 | GLY | SER | HIS | MET | PRO | GLU | SER | GLN | VAL | LYS | ||||
| 2 | SER | ASN | TYR | THR | ARG | GLY | ASN | GLN | LYS | SER | ||||
| 3 | SER | PHE | THR | GLU | ILE | LYS | ASP | SER | ALA | SER | ||||
| 4 | LYS | PRO | LYS | LYS | ASN | ALA | LEU | PRO | SER | SER | ||||
| 5 | THR | SER | THR | SER | ALA | PRO | VAL | SER | SER | THR | ||||
| 6 | ASN | LYS | VAL | PRO | GLN | ASN | GLY | SER | GLY | ASN | ||||
| 7 | SER | THR | SER | ILE | TYR | LEU | GLU | PRO | THR | PHE | ||||
| 8 | ASN | VAL | PRO | SER | SER | GLU | LEU | TYR | GLU | THR | ||||
| 9 | PHE | LEU | ASP | LYS | GLN | ARG | ILE | LEU | ALA | TRP | ||||
| 10 | THR | ARG | SER | ALA | GLN | PHE | PHE | ASN | SER | GLY | ||||
| 11 | PRO | LYS | LEU | GLU | THR | LYS | GLU | LYS | PHE | GLU | ||||
| 12 | LEU | PHE | GLY | GLY | ASN | VAL | ILE | SER | GLU | LEU | ||||
| 13 | VAL | SER | CYS | GLU | LYS | ASP | LYS | LYS | LEU | VAL | ||||
| 14 | PHE | HIS | TRP | LYS | LEU | LYS | ASP | TRP | SER | ALA | ||||
| 15 | PRO | PHE | ASN | SER | THR | ILE | GLU | MET | THR | PHE | ||||
| 16 | HIS | GLU | SER | GLN | GLU | PHE | HIS | GLU | THR | LYS | ||||
| 17 | LEU | GLN | VAL | LYS | TRP | THR | GLY | ILE | PRO | VAL | ||||
| 18 | GLY | GLU | GLU | ASP | ARG | VAL | ARG | ALA | ASN | PHE | ||||
| 19 | GLU | GLU | TYR | TYR | VAL | ARG | SER | ILE | LYS | LEU | ||||
| 20 | THR | PHE | GLY | PHE | GLY | ALA | VAL | LEU |
Related Database Links:
| DBJ | GAA22442 |
| EMBL | CAA92357 CAA92365 CAY78716 |
| GB | AAA73862 AAS56020 AHY75197 AJP37925 AJU58047 |
| REF | NP_010500 |
| SP | Q12449 |
| TPG | DAA12058 |
| AlphaFold | Q12449 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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