BMRB Entry 16628

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16628

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sequence-specific 1H, 13C and 15N resonance assignments of the nucleotide-free wildtype cyclic nucleotide binding domain from a cyclic nucleotide-activated K+ channel

Deposition date:

2009-12-06

Original release date:

2010-07-19

Authors:

Schuenke, Sven; Lecher, Justin; Stoldt, Matthias; Willbold, Dieter

Citation:

Citation: Schunke, Sven; Lecher, Justin; Stoldt, Matthias; Kaupp, U. Benjamin; Willbold, Dieter. "Resonance assignments of the nucleotide-free wildtype MloK1 cyclic nucleotide-binding domain." Biomol. NMR Assignments 4, 147-150 (2010).
PubMed: 20449776

Assembly members:

Assembly members:
MloK1_CNBD, polymer, 142 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mesorhizobium loti Taxonomy ID: 381 Superkingdom: Bacteria Kingdom: not available Genus/species: Mesorhizobium loti

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MloK1_CNBD: GSQEVRRGDFVRNWQLVAAV PLFQKLGPAVLVEIVRALRA RTVPAGAVICRIGEPGDRMF FVVEGSVSVATPNPVELGPG AFFGEMALISGEPRSATVSA ATTVSLLSLHSADFQMLCSS SPEIAEIFRKTALERRGAAA SA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	540
15N chemical shifts	134
1H chemical shifts	930

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MloK1 CNBD monomer	1

Entities:

Entity 1, MloK1 CNBD monomer 142 residues - Formula weight is not available

Residues 214-215 represent the cleavage hang over of an affinity GST-tag

1

GLY

SER

GLN

GLU

VAL

ARG

GLY

ASP

PHE

2

VAL

ARG

ASN

TRP

GLN

LEU

VAL

ALA

VAL

3

PRO

LEU

PHE

GLN

LYS

LEU

GLY

PRO

ALA

VAL

4

LEU

VAL

GLU

ILE

VAL

ARG

ALA

LEU

ARG

ALA

5

ARG

THR

VAL

PRO

ALA

GLY

ALA

VAL

ILE

CYS

6

ARG

ILE

GLY

GLU

PRO

GLY

ASP

ARG

MET

PHE

7

PHE

VAL

GLU

GLY

SER

VAL

SER

VAL

ALA

8

THR

PRO

ASN

PRO

VAL

GLU

LEU

GLY

PRO

GLY

9

ALA

PHE

GLY

GLU

MET

ALA

LEU

ILE

SER

10

GLY

GLU

PRO

ARG

SER

ALA

THR

VAL

SER

ALA

11

ALA

THR

VAL

SER

LEU

SER

LEU

HIS

12

SER

ALA

ASP

PHE

GLN

MET

LEU

CYS

SER

13

SER

PRO

GLU

ILE

ALA

GLU

ILE

PHE

ARG

LYS

14

THR

ALA

LEU

GLU

ARG

GLY

ALA

15

SER

ALA

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HNHA	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HCCH-COSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1

BMRB	15249 18024
PDB	1U12 1VP6 2K0G 2KXL 2ZD9 3BEH 3CL1 3CLP 3CO2 4CHV 4CHW 4MUV
DBJ	BAB50178
REF	WP_010911524 WP_032931689
SP	Q98GN8
AlphaFold	Q98GN8

BMRB Entry 16628

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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