BMRB Entry 16683

Assembly:

Entities:

Entity 1, REF_54-155 124 residues - 13596.237 Da.

Construct contains the following non-REF sequences: N-terminal T7 tag: MASMTGGQQMGRDP (assigned residue numbers 40-53) C-terminal His tag: LEHHHHHH (assigned residue numbers 156-163)

Entity 2, ICP27_103-138 40 residues - 4268.970 Da.

The construct contains the following non-ICP27 sequence remaining from protease cleavage site at N-termini: GPLG (assigned residue numbers 99-102)

Samples:

sample_1: REF 54-155, [U-99% 13C; U-99% 15N], 1 ± 0.05 mM; ICP27 103-138 2 ± 0.05 mM; Na phosphate buffer 20 mM; NaCl 50 mM; L-Arg 50 mM; L-Glu 50 mM; 2-mercaptoethanol 50 mM; DTT 10 mM; EDTA 10 mM; H2O 90%; D2O 10%

sample_2: ICP27 103-138, [U-99% 13C; U-99% 15N], 1 ± 0.05 mM; REF 54-155 2 ± 0.05 mM; Na phosphate buffer 20 mM; NaCl 50 mM; L-Arg 50 mM; L-Glu 50 mM; 2-mercaptoethanol 50 mM; DTT 10 mM; EDTA 10 mM; H2O 90%; D2O 10%

sample_conditions: ionic strength: 50 mM; pH: 6.2; pressure: 1 atm; temperature: 303 K

Experiments:

Software:

CYANA v2.1, P.GUNTERT ET AL. - structure solution

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

• Bruker DRX 600 MHz
• Bruker Avance II+ 700 MHz
• Varian INOVA 800 MHz