BMRB Entry 16697

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16697

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Title:
RRM domain of mRNA export adaptor REF2-I backbone assignment
Deposition date:
2010-01-29
Original release date:
2011-01-25
Authors:
Tunnicliffe, Richard; Golovanov, Alexander; Wilson, Stuart; Hautbergue, Guillaume
Citation:

Citation: Tunnicliffe, Richard; Hautbergue, Guillaume; Kalra, Priti; Jackson, Brian; Whitehouse, Adrian; Wilson, Stuart; Golovanov, Alexander. "Structural Basis for the Recognition of Cellular mRNA Export Factor REF by Herpes Viral Proteins HSV-1 ICP27 and HVS ORF57."  PLoS Pathog. 7, e1001244-. (2011).
PubMed: 21253573

Assembly members:

Assembly members:
REF_54-155, polymer, 124 residues, 13596.237 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
REF_54-155: MASMTGGQQMGRDPDKWQHD LFDSGCGGGEGVETGAKLLV SNLDFGVSDADIQELFAEFG TLKKAAVDYDRSGRSLGTAD VHFERRADALKAMKQYKGVP LDGRPMDIQLVASQIDLEHH HHHH

Data sets:
Data typeCount
13C chemical shifts325
15N chemical shifts113
1H chemical shifts113

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1REF_54-1551

Entities:

Entity 1, REF_54-155 124 residues - 13596.237 Da.

Construct contains the following non-REF sequences: N-terminal T7 tag: MASMTGGQQMGRDP (assigned residue numbers 40-53) C-terminal His tag: LEHHHHHH (assigned residue numbers 156-163)

1   METALASERMETTHRGLYGLYGLNGLNMET
2   GLYARGASPPROASPLYSTRPGLNHISASP
3   LEUPHEASPSERGLYCYSGLYGLYGLYGLU
4   GLYVALGLUTHRGLYALALYSLEULEUVAL
5   SERASNLEUASPPHEGLYVALSERASPALA
6   ASPILEGLNGLULEUPHEALAGLUPHEGLY
7   THRLEULYSLYSALAALAVALASPTYRASP
8   ARGSERGLYARGSERLEUGLYTHRALAASP
9   VALHISPHEGLUARGARGALAASPALALEU
10   LYSALAMETLYSGLNTYRLYSGLYVALPRO
11   LEUASPGLYARGPROMETASPILEGLNLEU
12   VALALASERGLNILEASPLEUGLUHISHIS
13   HISHISHISHIS

Related Database Links:

BMRB 16683 17693
PDB 2KT5 2YKA

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks